Understand the Functions of Scaffold Proteins in Cell Signaling by a Mesoscopic Simulation Method.

abstract：:Microtubules are cytoskeletal filaments whose self-assembly occurs by abrupt switching between states of roughly constant growth and shrinkage, a process known as dynamic instability. Understanding the mechanism of dynamic instability offers potential for controlling microtubule-dependent cellular processes such as ne...

journal_title：Biophysical journal

authors： Odde DJ,Cassimeris L,Buettner HM

更新日期：1995-09-01 00:00:00

abstract：:The release of GDP from GTPases signals the initiation of a GTPase cycle, where the association of GTP triggers conformational changes promoting binding of downstream effector molecules. Studies have implicated the nucleotide-binding G5 loop to be involved in the GDP release mechanism. For example, biophysical studies...

journal_title：Biophysical journal

authors： Guilfoyle AP,Deshpande CN,Font Sadurni J,Ash MR,Tourle S,Schenk G,Maher MJ,Jormakka M

更新日期：2014-12-16 00:00:00

abstract：:We have explored the electromechanical properties of DNA on a nanometer-length scale using an electric field to force single molecules through synthetic nanopores in ultrathin silicon nitride membranes. At low electric fields, E < 200 mV/10 nm, we observed that single-stranded DNA can permeate pores with a diameter >/...

journal_title：Biophysical journal

authors： Heng JB,Aksimentiev A,Ho C,Marks P,Grinkova YV,Sligar S,Schulten K,Timp G

更新日期：2006-02-01 00:00:00

abstract：:A variety of amphiphilic helical peptides have been shown to exhibit a transition from adsorbing parallel to a membrane surface at low concentrations to inserting perpendicularly into the membrane at high concentrations. Furthermore, this transition has been correlated to the peptides' cytolytic activities. X-ray lame...

journal_title：Biophysical journal

authors： Wu Y,He K,Ludtke SJ,Huang HW

更新日期：1995-06-01 00:00:00

abstract：:A voltage clamp study has been performed in the presynaptic terminal of the squid stellate ganglion. After blockage of the voltage-dependent sodium and potassium conductances, an inward calcium current is demonstrated. Given a step-depolarization pulse, this voltage- and time-dependent conductance has an S-shaped onse...

journal_title：Biophysical journal

authors： Llinás R,Steinberg IZ,Walton K

更新日期：1981-03-01 00:00:00

abstract：:Stationary and nonstationary correlation-frequency analysis of heterodyne laser light scattering were utilized to make automated, on-line, objective measurements of tracheal ciliary beat frequency (CBF) in intact, anesthetized canines. The stationary correlation-frequency analysis laser light-scattering technique was ...

journal_title：Biophysical journal

authors： Chandra T,Yeates DB,Miller IF,Wong LB

更新日期：1994-03-01 00:00:00

abstract：:From the study of the kinetics of the fluorescence self-quenching of pyrene in multilamellar vesicles of 1,2-dimyristoyl-sn-glycero-3-phosphocholine (DMPC) in the fluid phase we obtain the molecular diffusion parameters, diffusion coefficients, and activation energies for lateral diffusion for three probes: 1-palmitoy...

journal_title：Biophysical journal

authors： Martins J,Melo E

更新日期：2001-02-01 00:00:00

abstract：:Molecular motors are thought to generate force and directional motion via nonequilibrium switching between energy surfaces. Because all enzymes can undergo such switching, we hypothesized that the ability to generate rotary motion and torque is not unique to highly adapted biological motor proteins but is instead a co...

journal_title：Biophysical journal

authors： Slochower DR,Gilson MK

更新日期：2018-05-08 00:00:00

abstract：:Using homonuclear (1)H NOESY spectra, with chemical shifts, (3)JH(N)H(α) scalar couplings, residual dipolar couplings, and (1)H-(15)N NOEs, we have optimized and validated the conformational ensembles of the amyloid-β 1-40 (Aβ40) and amyloid-β 1-42 (Aβ42) peptides generated by molecular dynamics simulations. We find t...

journal_title：Biophysical journal

authors： Ball KA,Phillips AH,Wemmer DE,Head-Gordon T

更新日期：2013-06-18 00:00:00

abstract：:The distribution of low concentrations of ganglioside GM1 in L-alpha-dipalmitoylphosphatidylcholine (DPPC) and DPPC/cholesterol monolayers supported on mica has been studied using atomic force microscopy (AFM). The monolayers studied correspond to a pure gel phase and a mixture of liquid-expanded (LE) and liquid-conde...

journal_title：Biophysical journal

authors： Yuan C,Johnston LJ

更新日期：2000-11-01 00:00:00

abstract：:The diffusion of Ca as it converges to the external mouth of a Ca channel is examined. Diffusional limitation on Ca ions entering Ca channels during current flow, cause local extracellular Ca depletions. Such extracellular Ca depletions have been reported in cardiac muscle. The cardiac sarcolemma has a large number of...

journal_title：Biophysical journal

authors： Bers DM,Peskoff A

更新日期：1991-03-01 00:00:00

abstract：:The sarcoplasmic reticulum channel (ryanodine receptor) from cardiac myocytes was reconstituted into planar lipid bilayers consisting of 1-palmitoyl-2-oleoyl-phosphatidylethanolamine (POPE) and 1-palmitoyl-2-oleoyl-phosphatidylcholine (POPC) in varying ratios. The channel activity parameters, i.e., open probability an...

journal_title：Biophysical journal

authors： Cannon B,Hermansson M,Györke S,Somerharju P,Virtanen JA,Cheng KH

更新日期：2003-08-01 00:00:00

abstract：:Binding of a small molecule to a macromolecular target reduces its conformational freedom, resulting in a negative entropy change that opposes the binding. The goal of this study is to estimate the configurational entropy change of two minor-groove-binding ligands, netropsin and distamycin, upon binding to the DNA dup...

journal_title：Biophysical journal

authors： Dolenc J,Baron R,Oostenbrink C,Koller J,van Gunsteren WF

更新日期：2006-08-15 00:00:00

abstract：:We have examined the morphology and osmotic properties of large unilamellar vesicles (LUVs) prepared by extrusion. Contrary to expectations, we observe by cryo-electron microscopy that such vesicles, under isoosmotic conditions, are non-spherical. This morphology appears to be a consequence of vesicle passage through ...

journal_title：Biophysical journal

authors： Mui BL,Cullis PR,Evans EA,Madden TD

更新日期：1993-02-01 00:00:00

abstract：:Ion channels enable diffusion of ions down physiological electrochemical gradients. Modulation of ion permeation is crucial for the physiological functioning of cells, and misregulation of ion channels is linked to a myriad of channelopathies. The ion permeation mechanism in the transient receptor potential (TRP) ion ...

journal_title：Biophysical journal

authors： Jorgensen C,Furini S,Domene C

更新日期：2016-09-20 00:00:00

abstract：:The role of distance-dependent anisotropic reactivity and molecular geometry in the vicinity of localized reaction centers in influencing the rate of bimolecular diffusion-controlled reactions is analyzed in detail, both analytically and numerically. The effect of local molecular shape is considered within the model o...

journal_title：Biophysical journal

authors： Shushin AI,Barzykin AV

更新日期：2001-12-01 00:00:00

abstract：:Membranes of the lens of the eye of mammals have two particular characteristics, high concentrations of sphingomyelin, and dihydrosphingomyelin and cholesterol. We have studied the miscibility of cholesterol with both egg sphingomyelin and with dihydrosphingomyelin made by hydrogenation of egg sphingomyelin. At a chol...

journal_title：Biophysical journal

authors： Epand RM

更新日期：2003-05-01 00:00:00

abstract：:In adherent and motile neutrophils NAD(P)H concentration, flavoprotein redox potential, and production of reactive oxygen species and nitric oxide, are all periodic and exhibit defined phase relationships to an underlying metabolic oscillation of approximately 20 s. Utilizing fluorescence microscopy, we have shown in ...

journal_title：Biophysical journal

authors： Rosenspire AJ,Kindzelskii AL,Simon BJ,Petty HR

更新日期：2005-05-01 00:00:00

abstract：:In most studied microbial rhodopsins two conserved carboxylic acid residues (the homologs of Asp-85 and Asp-212 in bacteriorhodopsin) and an arginine residue (the homolog of Arg-82) form a complex counterion to the protonated retinylidene Schiff base, and neutralization of the negatively charged carboxylates causes re...

journal_title：Biophysical journal

authors： Li H,Govorunova EG,Sineshchekov OA,Spudich JL

更新日期：2014-04-15 00:00:00

abstract：:Closing linear poly(ethylene glycol) (PEG) into a circular "crown" dramatically changes its dynamics in the alpha-hemolysin channel. In the electrically neutral crown ether (C2H4O)6, six ethylene oxide monomers are linked into a circle that gives the molecule ion-complexing capacity and increases its rigidity. As with...

journal_title：Biophysical journal

authors： Bezrukov SM,Krasilnikov OV,Yuldasheva LN,Berezhkovskii AM,Rodrigues CG

更新日期：2004-11-01 00:00:00

abstract：:The reported rates of thermal 13-cis to all-trans isomerization of the protonated Schiff base of retinal (PSBR) in solution and in bacteriorhodopsin (BR) are shown to be correlated with the red shift in the absorption maximum of the chromophore, though the linear fit is different for BR and for a model PSBR in solutio...

journal_title：Biophysical journal

authors： Milder SJ

更新日期：1991-08-01 00:00:00

abstract：:Fluorescence lifetimes of dimeric rabbit muscle creatine kinase specifically dansylated at both active sites and the homologous monomeric lobster muscle arginine kinase singly dansylated were determined using phase-modulation methods with global analysis of overdetermined data sets. For both proteins, the data is adeq...

journal_title：Biophysical journal

authors： Grossman SH

更新日期：1991-03-01 00:00:00

abstract：:O-acetylserine sulfhydrylase, a homo-dimeric enzyme from Salmonella typhimurium, covalently binds one pyridoxal 5'-phosphate molecule per subunit as a fluorescent coenzyme. Different tautomers of the Schiff base between the coenzyme and lysine 41 generate structured absorption and fluorescence spectra upon one-photon ...

journal_title：Biophysical journal

authors： Chirico G,Bettati S,Mozzarelli A,Chen Y,Müller JD,Gratton E

更新日期：2001-04-01 00:00:00

abstract：:Human apolipoprotein E (apoE) is a 299-amino-acid protein with a molecular weight of 34 kDa. The difference between the apoE3 and apoE4 isoforms is a single residue substitution involving a Cys-Arg replacement at residue 112. ApoE4 is positively associated with atherosclerosis and late-onset and sporadic Alzheimer's d...

journal_title：Biophysical journal

authors： Chou CY,Lin YL,Huang YC,Sheu SY,Lin TH,Tsay HJ,Chang GG,Shiao MS

更新日期：2005-01-01 00:00:00

abstract：:The Ca(2+)-permeable cation channel TRPA1 acts as an ionotropic receptor for various pungent compounds and as a noxious cold sensor in sensory neurons. It is unclear what proportion of the TRPA1-mediated current is carried by Ca(2+) ions and how the permeation pathway changes during stimulation. Here, based on the rel...

journal_title：Biophysical journal

authors： Karashima Y,Prenen J,Talavera K,Janssens A,Voets T,Nilius B

更新日期：2010-03-03 00:00:00

abstract：:The following arguments are presented for the observation that curves relating free Ca2+ and force development of thin filament regulated myofilaments of skinned muscle fibers have Hill coefficient (n) greater than 4, which is the number of Ca2+ binding sites on troponin: Activation of the myofilaments is a process re...

journal_title：Biophysical journal

authors： Shiner JS,Solaro RJ

更新日期：1984-10-01 00:00:00

abstract：:The outer membrane (OM) of Gram-negative bacteria is composed of lipopolysaccharide (LPS) in the outer leaflet and phospholipids in the inner leaflet. The outer membrane protein H (OprH) of Pseudomonas aeruginosa provides an increased stability to the OMs by directly interacting with LPS. Here we report the influence ...

journal_title：Biophysical journal

authors： Lee J,Patel DS,Kucharska I,Tamm LK,Im W

更新日期：2017-01-24 00:00:00

abstract：:A detailed analysis is made of the model system of two parallel cylindrical polyelectrolytes which contain ionizable groups on their surfaces and are immersed in an ionic bathing medium. The interaction between the cylinders is examined by considering the interplay between repulsive electrostatic forces and attractive...

journal_title：Biophysical journal

authors： Brenner SL,McQuarrie DA

更新日期：1973-04-01 00:00:00

abstract：:Platinum-containing molecules are widely used as anticancer drugs. These molecules exert cytotoxic effects by binding to DNA through various mechanisms. The binding between DNA and platinum-based drugs hinders the opening of DNA, and therefore, DNA duplication and transcription are severely hampered. Overall, impeding...

journal_title：Biophysical journal

authors： Salerno D,Beretta GL,Zanchetta G,Brioschi S,Cristofalo M,Missana N,Nardo L,Cassina V,Tempestini A,Giovannoni R,Cerrito MG,Zaffaroni N,Bellini T,Mantegazza F

更新日期：2016-05-24 00:00:00

abstract：:We used optical tweezers to analyze the effect of jasplakinolide and cyclodextrin on the force exerted by lamellipodia from developing growth cones (GCs) of isolated dorsal root ganglia (DRG) neurons. We found that 25 nM of jasplakinolide, which is known to inhibit actin filament turnover, reduced both the maximal exe...

journal_title：Biophysical journal

authors： Amin L,Ercolini E,Shahapure R,Migliorini E,Torre V

更新日期：2012-06-06 00:00:00