Energy transfer and charge separation kinetics in photosystem I: Part 1: Picosecond transient absorption and fluorescence study of cyanobacterial photosystem I particles.

abstract：:A challenge in the understanding of creatine kinase (CK) fluxes reflected by NMR magnetization transfer in the perfused rat heart is the choice of a kinetic model of analysis. The complexity of the energetic pathways, due to the presence of adenosine triphosphate (ATP)-inorganic phosphate (Pi) exchange, of metabolite ...

journal_title：Biophysical journal

authors： Joubert F,Hoerter JA,Mazet JL

更新日期：2001-12-01 00:00:00

abstract：:Adenylate kinase, an enzyme that catalyzes the phosphoryl transfer between ATP and AMP, can interconvert between the open and catalytically potent (closed) forms even without binding ligands. Several aspects of the enzyme elasticity and internal dynamics are analyzed here by atomistic molecular dynamics simulations co...

journal_title：Biophysical journal

authors： Pontiggia F,Zen A,Micheletti C

更新日期：2008-12-15 00:00:00

abstract：:We present a method for calculating the configurational-dependent diffusion coefficient of a globular protein as a function of the global folding process. Using a coarse-grained structure-based model, we determined the diffusion coefficient, in reaction coordinate space, as a function of the fraction of native contact...

journal_title：Biophysical journal

authors： Oliveira RJ,Whitford PC,Chahine J,Wang J,Onuchic JN,Leite VB

更新日期：2010-07-21 00:00:00

abstract：:A complete mechanistic understanding of membrane-localized processes in vesicular transport, such as membrane budding and fission, requires their reconstitution with biochemically-defined components from a biochemically-defined substrate. Supported bilayers formed by vesicle fusion represent an attractive substrate fo...

journal_title：Biophysical journal

authors： Pucadyil TJ,Schmid SL

更新日期：2010-07-21 00:00:00

abstract：:Dendrites of cerebellar Purkinje cells (PCs) respond to brief excitations from parallel fibers with lasting plateau depolarizations. It is unknown whether these plateaus are local events that boost the synaptic signals or they propagate to the soma and directly take part in setting the cell firing dynamics. To address...

journal_title：Biophysical journal

authors： Genet S,Sabarly L,Guigon E,Berry H,Delord B

更新日期：2010-07-21 00:00:00

abstract：:In cardiac tissue, during partial blockade of the membrane sodium channels, or at high frequencies of excitation, inexcitable obstacles with sharp edges may destabilize the propagation of electrical excitation waves, causing the formation of self-sustained vortices and turbulent cardiac electrical activity. The format...

journal_title：Biophysical journal

authors： Cabo C,Pertsov AM,Davidenko JM,Baxter WT,Gray RA,Jalife J

更新日期：1996-03-01 00:00:00

abstract：:The S4 segments of voltage-gated sodium channels are important parts of the voltage-sensing elements of these proteins. Furthermore, the addition of the isolated S4 polypeptide to planar lipid bilayers results in stepwise increases of ion conductivity. In order to gain insight into the mechanisms of pore formation by ...

journal_title：Biophysical journal

authors： Mattila K,Kinder R,Bechinger B

更新日期：1999-10-01 00:00:00

abstract：:Thermodynamic parameters for the binding of the monovalent cations, Li+, Na+, K+, Rb+, Cs+, NH4+, TI+, and Ag+, to gramicidin A and for the binding of TI+ to gramicidin C, incorporated into lysophosphatidylcholine, have been determined using a combination of TI-205 nuclear magnetic resonance spectroscopy and competiti...

journal_title：Biophysical journal

authors： Hinton JF,Fernandez JQ,Shungu DC,Whaley WL,Koeppe RE 2nd,Millett FS

更新日期：1988-09-01 00:00:00

abstract：:An experimental method and analysis are introduced which provide direct quantitation of the strength of adhesive contact for large agglutinin-bonded regions between macroscopically smooth membrane capsules (e.g., red blood cells). The approach yields intrinsic properties for separation of adherent regions independent ...

journal_title：Biophysical journal

authors： Berk D,Evans E

更新日期：1991-04-01 00:00:00

abstract：:In rat mesenteric arteries, smooth muscle cells exhibit intercellular calcium waves in response to local phenylephrine stimulation. These waves have a velocity of approximately 20 cells/s and a range of approximately 80 cells. We analyze these waves in a theoretical model of a population of coupled smooth muscle cells...

journal_title：Biophysical journal

authors： Koenigsberger M,Seppey D,Bény JL,Meister JJ

更新日期：2010-07-21 00:00:00

abstract：:Hormones are released from neuroendocrine cells by passing through an exocytotic pore that forms after vesicle and plasma membrane fusion. An elegant way to study this process at the single-vesicle level is to use styryl dyes, which stain not only the membrane, but also the matrix of individual vesicles in some neuroe...

journal_title：Biophysical journal

authors： Stenovec M,Poberaj I,Kreft M,Zorec R

更新日期：2005-04-01 00:00:00

abstract：:The intracellular and interstitial potentials associated with each cell or fiber in multicellular preparations carrying a uniformly propagating wave are important for characterizing the electrophysiological behavior of the preparation and in particular, for evaluating the source contributed by each fiber. The aforemen...

journal_title：Biophysical journal

authors： Henriquez CS,Trayanova N,Plonsey R

更新日期：1988-06-01 00:00:00

abstract：:In the last decades, it has been demonstrated that many animal species orient in the Earth magnetic field. One of the best-studied examples is the use of the geomagnetic field by migratory birds for orientation and navigation. However, the biophysical mechanism underlying animal magnetoreception is still not understoo...

journal_title：Biophysical journal

authors： Solov'yov IA,Schulten K

更新日期：2009-06-17 00:00:00

abstract：:A kinetic model of the effect of agonist and anesthetics on ligand-gated ion channels, developed in earlier work, is further refined and used to predict traces observed in fast-perfusion electrophysiological studies on recombinant GABAA receptors under a wide range of agonist and/or anesthetic concentrations. The mode...

journal_title：Biophysical journal

authors： Lee DK,Albershardt DJ,Cantor RS

更新日期：2015-03-10 00:00:00

abstract：:Hair cells of the inner ear can power spontaneous oscillations of their mechanosensory hair bundle, resulting in amplification of weak inputs near the characteristic frequency of oscillation. Recently, dynamic force measurements have revealed that delayed gating of the mechanosensitive ion channels responsible for mec...

journal_title：Biophysical journal

authors： Barral J,Jülicher F,Martin P

更新日期：2018-01-23 00:00:00

abstract：:Plexins are single-pass transmembrane receptors that bind the axon guidance molecules semaphorins. Single-pass transmembrane proteins are an important class of receptors that display a wide variety of activation mechanisms, often involving ligand-dependent dimerization or conformational changes. Resolving the activati...

journal_title：Biophysical journal

authors： Marita M,Wang Y,Kaliszewski MJ,Skinner KC,Comar WD,Shi X,Dasari P,Zhang X,Smith AW

更新日期：2015-11-03 00:00:00

abstract：:The conformation of the polypeptide melittin in lipid membranes as determined by Raman spectroscopy is a bent alpha-helix formed by the mainly hydrophobic residues 1-21, and a nonhelical COOH-terminal segment of the hydrophilic residues 22-26. Fluorescence quenching experiments on residue Trp19 reveal that all COOH-te...

journal_title：Biophysical journal

authors： Vogel H,Jähnig F

更新日期：1986-10-01 00:00:00

abstract：:We introduce an intrinsically multiplexed and easy to implement method to apply an external force to a biomolecule and thus probe its interaction with a second biomolecule or, more generally, its environment (for example, the cell membrane). We take advantage of the hydrodynamic interaction with a controlled fluid flo...

journal_title：Biophysical journal

authors： Türkcan S,Richly MU,Bouzigues CI,Allain JM,Alexandrou A

更新日期：2013-07-02 00:00:00

abstract：:Catch bonds are characterized by average lifetimes that initially increase with increasing tensile force. Recently, they have been implicated in T cell activation, where small numbers of antigenic receptor-ligand bonds at a cell-cell interface can stimulate a T cell. Here, we use computational methods to investigate s...

journal_title：Biophysical journal

authors： Pullen RH 3rd,Abel SM

更新日期：2017-07-11 00:00:00

abstract：:Detection of the transition between the two myosin isoforms α- and β-myosin in living cardiomyocytes is essential for understanding cardiac physiology and pathology. In this study, the differences in symmetry of polarization spectra obtained from α- and β-myosin in various mammalian ventricles and propylthiouracil-tre...

journal_title：Biophysical journal

authors： Yuan C,Zhao X,Wang Z,Borg TK,Ye T,Khalpey ZI,Runyan RB,Shao Y,Gao BZ

更新日期：2020-03-10 00:00:00

abstract：:The endoplasmic reticulum (ER) in plant cells forms a highly dynamic network of complex geometry. ER network morphology and dynamics are influenced by a number of biophysical processes, including filament/tubule tension, viscous forces, Brownian diffusion, and interactions with many other organelles and cytoskeletal e...

journal_title：Biophysical journal

authors： Lin C,White RR,Sparkes I,Ashwin P

更新日期：2017-07-11 00:00:00

abstract：:Combining an azobenzene chromophore with the bis-cysteinyl active-site sequence of the protein disulfide isomerase (PDI) we constructed a simple but promising model for allosteric conformational rearrangements. Paralleling cellular signaling events, an external trigger, here absorption of a photon, leads to a structur...

journal_title：Biophysical journal

authors： Löweneck M,Milbradt AG,Root C,Satzger H,Zinth W,Moroder L,Renner C

更新日期：2006-03-15 00:00:00

abstract：:Electrical bursting oscillations of mammalian pancreatic beta-cells are synchronous among cells within an islet. While electrical coupling among cells via gap junctions has been demonstrated, its extent and topology are unclear. The beta-cells also share an extracellular compartment in which oscillations of K+ concent...

journal_title：Biophysical journal

authors： Stokes CL,Rinzel J

更新日期：1993-08-01 00:00:00

abstract：:Skeletal muscle contraction is a canonical example of motor-driven force generation. Despite the long history of research in this topic, a mechanistic explanation of the collective myosin force generation is lacking. We present a theoretical model of muscle contraction based on the conformational movements of individu...

journal_title：Biophysical journal

authors： Lan G,Sun SX

更新日期：2005-06-01 00:00:00

abstract：:13C alpha chemical shifts and site-specific unfolding curves are reported for 12 sites on a 33-residue, GCN4-like leucine zipper peptide (GCN4-lzK), ranging over most of the chain and sampling most heptad positions. Data were derived from NMR spectra of nine synthetic, isosequential peptides bearing 99% 13C alpha at s...

journal_title：Biophysical journal

authors： Holtzer ME,Lovett EG,d'Avignon DA,Holtzer A

更新日期：1997-08-01 00:00:00

abstract：:The motility of Amoeba proteus was examined using the technique of passive particle tracking microrheology, with the aid of newly developed particle tracking software, a fast digital camera, and an optical microscope. We tracked large numbers of endogeneous particles in the amoebae, which displayed subdiffusive motion...

journal_title：Biophysical journal

authors： Rogers SS,Waigh TA,Lu JR

更新日期：2008-04-15 00:00:00

abstract：:Dithioation of DNA phosphate is known to enhance binding affinities, at least for some proteins. We mechanistically characterized this phenomenon for the Antennapedia homeodomain-DNA complex by integrated use of fluorescence, isothermal titration calorimetry, NMR spectroscopy, and x-ray crystallography. By fluorescenc...

journal_title：Biophysical journal

authors： Zandarashvili L,Nguyen D,Anderson KM,White MA,Gorenstein DG,Iwahara J

更新日期：2015-09-01 00:00:00

abstract：:Cellular differentiation proceeds along complicated pathways, even when it is induced by extracellular signaling molecules. One of the major reasons for this complexity is the highly multidimensional internal dynamics of cells, which sometimes causes apparently stochastic responses in individual cells to extracellular...

journal_title：Biophysical journal

authors： Morita S,Takanezawa S,Hiroshima M,Mitsui T,Ozaki Y,Sako Y

更新日期：2014-11-18 00:00:00

abstract：:Nonlinear optical Stokes ellipsometric (NOSE) microscopy was demonstrated for the analysis of collagen-rich biological tissues. NOSE is based on polarization-dependent second harmonic generation imaging. NOSE was used to access the molecular-level distribution of collagen fibril orientation relative to the local fiber...

journal_title：Biophysical journal

authors： Dow XY,DeWalt EL,Sullivan SZ,Schmitt PD,Ulcickas JRW,Simpson GJ

更新日期：2016-10-04 00:00:00

abstract：:Cytoplasmic free Mg(2+) concentration ([Mg(2+)]i) was measured in rat ventricular myocytes with a fluorescent indicator furaptra (mag-fura-2) introduced by AM-loading. By incubation of the cells in a high-K(+) (Ca(2+)- and Mg(2+)-free) solution, [Mg(2+)]i decreased from ? 0.9 mM to 0.2 to 0.5 mM. The lowered [Mg(2+)]i...

journal_title：Biophysical journal

authors： Tashiro M,Inoue H,Konishi M

更新日期：2014-11-04 00:00:00