Abstract:
:Nonlinear optical Stokes ellipsometric (NOSE) microscopy was demonstrated for the analysis of collagen-rich biological tissues. NOSE is based on polarization-dependent second harmonic generation imaging. NOSE was used to access the molecular-level distribution of collagen fibril orientation relative to the local fiber axis at every position within the field of view. Fibril tilt-angle distribution was investigated by combining the NOSE measurements with ab initio calculations of the predicted molecular nonlinear optical response of a single collagen triple helix. The results were compared with results obtained previously by scanning electron microscopy, nuclear magnetic resonance imaging, and electron tomography. These results were enabled by first measuring the laboratory-frame Jones nonlinear susceptibility tensor, then extending to the local-frame tensor through pixel-by-pixel corrections based on local orientation.
journal_name
Biophys Jjournal_title
Biophysical journalauthors
Dow XY,DeWalt EL,Sullivan SZ,Schmitt PD,Ulcickas JRW,Simpson GJdoi
10.1016/j.bpj.2016.05.055subject
Has Abstractpub_date
2016-10-04 00:00:00pages
1361-1374issue
7eissn
0006-3495issn
1542-0086pii
S0006-3495(16)30700-7journal_volume
111pub_type
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