Thick-Filament Extensibility in Intact Skeletal Muscle.

abstract：:Reverberating spontaneous synchronized brain activity is believed to play an important role in neural information processing. Whether and how external stimuli can influence this spontaneous activity is poorly understood. Because periodic synchronized network activity is also prominent in in vitro neuronal cultures, we...

journal_title：Biophysical journal

authors： Vajda I,van Pelt J,Wolters P,Chiappalone M,Martinoia S,van Someren E,van Ooyen A

更新日期：2008-06-01 00:00:00

abstract：:The dynamics of protein distribution in endocytic membranes are relevant for many cellular processes, such as protein sorting, organelle and membrane microdomain biogenesis, protein-protein interactions, receptor function, and signal transduction. We have developed an assay based on Fluorescence Resonance Energy Micro...

journal_title：Biophysical journal

authors： Wallrabe H,Elangovan M,Burchard A,Periasamy A,Barroso M

更新日期：2003-07-01 00:00:00

abstract：:A dynamic model of smooth muscle contraction is presented and is compared with the mechanical properties of vascular smooth muscle in the rat portal vein. The model is based on the sliding filament theory and the assumption that force is produced by cross-bridges extending from the myosin to the actin filaments. Thus,...

journal_title：Biophysical journal

authors： Gestrelius S,Borgström P

更新日期：1986-07-01 00:00:00

abstract：:Experimental approaches such as fiber diffraction and cryo-electron microscopy reconstruction have defined regulatory positions of tropomyosin on actin but have not, as yet, succeeded at determining key atomic-level contacts between these proteins or fully substantiated the dynamics of their interactions at a structur...

journal_title：Biophysical journal

authors： Pavadai E,Lehman W,Rynkiewicz MJ

更新日期：2020-07-07 00:00:00

abstract：:The results of variable dielectric coefficient Poisson-Boltzmann calculations of the counter-ion concentration in the vicinity of an all-atom model of the B-form of DNA are presented with an emphasis on the importance of spatial variations in the dielectric properties of the solvent, particularly at the macro-ion-solv...

journal_title：Biophysical journal

authors： Pack GR,Garrett GA,Wong L,Lamm G

更新日期：1993-10-01 00:00:00

abstract：:The binding mechanism of sweet proteins to their receptor, a G-protein-coupled receptor, is not supported by direct structural information. In principle, the key groups responsible for biological activity (glucophores) can be localized on a small structural unit (sweet finger) or spread on a larger surface area. A rec...

journal_title：Biophysical journal

authors： De Simone A,Spadaccini R,Temussi PA,Fraternali F

更新日期：2006-05-01 00:00:00

abstract：:The barbiturate pentobarbital binds to gamma-aminobutyric acid type A (GABA(A)) receptors, and this interaction plays an important role in the anesthetic action of this drug. Depending on its concentration, pentobarbital can potentiate (approximately 10-100 microM), activate (approximately 100-800 microM), or block (a...

journal_title：Biophysical journal

authors： Muroi Y,Theusch CM,Czajkowski C,Jackson MB

更新日期：2009-01-01 00:00:00

abstract：:The processes of cell growth and budding of the yeast cells Saccharomyces cerevisiae, which were gently immobilized on 3% agar and submerged in culture medium, were successfully imaged with an atomic force microscope for 6-7 h. Similar experiments on chemically fixed cells did not detect any appreciable change in thei...

journal_title：Biophysical journal

authors： Gad M,Ikai A

更新日期：1995-12-01 00:00:00

abstract：:Several investigators have demonstrated that under conditions where little or no gas exchange occurs across the alveolar capillary membrane the PCO(2) is higher in the alveolus than in the mixed venous blood, whereas there are no PO(2) differences. Gurtner et al. have explained the DeltaPCO(2) by a model in which H(+)...

journal_title：Biophysical journal

authors： Gurtner GH

更新日期：1972-06-01 00:00:00

abstract：:Prion diseases are infectious fatal neurodegenerative diseases including Creutzfeldt-Jakob disease in humans and bovine spongiform encephalopathy in cattle. The misfolding and conversion of cellular PrP in such mammals into pathogenic PrP is believed to be the key procedure. Rabbits are among the few mammalian species...

journal_title：Biophysical journal

authors： Zhou Z,Yan X,Pan K,Chen J,Xie ZS,Xiao GF,Yang FQ,Liang Y

更新日期：2011-09-21 00:00:00

abstract：:Toward quantitative description of protein aggregation, we develop a computationally efficient method to evaluate the potential of mean force between two folded protein molecules that allows for complete sampling of their mutual orientation. Our model is valid at moderate ionic strengths and accounts for the actual ch...

journal_title：Biophysical journal

authors： Chan HY,Lankevich V,Vekilov PG,Lubchenko V

更新日期：2012-04-18 00:00:00

abstract：:The molecular motor protein myosin VI moves toward the minus-end of actin filaments with a step size of 30-36 nm. Such large step size either drastically limits the degree of complex formation between dimer subunits to leave enough length for the lever arms, or requires an extension of the lever arms' crystallographic...

journal_title：Biophysical journal

authors： Liu Y,Hsin J,Kim H,Selvin PR,Schulten K

更新日期：2011-06-22 00:00:00

abstract：:The functional organization of prokaryotic cell membranes, which is essential for many cellular processes, has been challenging to analyze due to the small size and nonflat geometry of bacterial cells. Here, we use single-molecule fluorescence microscopy and three-dimensional quantitative analyses in live Escherichia ...

journal_title：Biophysical journal

authors： Oswald F,Varadarajan A,Lill H,Peterman EJ,Bollen YJ

更新日期：2016-03-08 00:00:00

abstract：:Experimental variables of optical tweezers instrumentation that affect RNA folding/unfolding kinetics were investigated. A model RNA hairpin, P5ab, was attached to two micron-sized beads through hybrid RNA/DNA handles; one bead was trapped by dual-beam lasers and the other was held by a micropipette. Several experimen...

journal_title：Biophysical journal

authors： Wen JD,Manosas M,Li PT,Smith SB,Bustamante C,Ritort F,Tinoco I Jr

更新日期：2007-05-01 00:00:00

abstract：:The glucose transporters (GLUT/SLC2A) are members of the major facilitator superfamily. Here, we generated a three-dimensional model for Glut1 using a two-step strategy: 1), GlpT structure as an initial homology template and 2), evolutionary homology using glucose-6-phosphate translocase as a template. The resulting s...

journal_title：Biophysical journal

authors： Salas-Burgos A,Iserovich P,Zuniga F,Vera JC,Fischbarg J

更新日期：2004-11-01 00:00:00

abstract：:A general analytical description of the equilibrium and reaction kinetics of DNA multiplex hybridization has been developed. In this approach, multiplex hybridization is considered to be a competitive multichannel reaction process: a system wherein many species can react both specifically and nonspecifically with one ...

journal_title：Biophysical journal

authors： Horne MT,Fish DJ,Benight AS

更新日期：2006-12-01 00:00:00

abstract：:The protonation state and intracellular distribution of ellipticine were investigated in single human mammary T47D cells by confocal laser microspectrofluorimetry. In the cell nucleus, only the protonated form of ellipticine was detected as a direct consequence of its apparent pK increase upon DNA binding. Both proton...

journal_title：Biophysical journal

authors： Sureau F,Moreau F,Millot JM,Manfait M,Allard B,Aubard J,Schwaller MA

更新日期：1993-11-01 00:00:00

abstract：:The cytoplasmic surface of the BR (initial) state of bacteriorhodopsin is characterized by a cluster of three carboxylates that function as a proton-collecting antenna. Systematic replacement of most of the surface carboxylates indicated that the cluster is made of D104, E161, and E234 (Checover, S., Y. Marantz, E. Na...

journal_title：Biophysical journal

authors： Nachliel E,Gutman M,Tittor J,Oesterhelt D

更新日期：2002-07-01 00:00:00

abstract：:Fourier transform infrared (FTIR) spectroscopy was used to monitor secondary structural changes associated with binding of bovine prothrombin and prothrombin fragment 1 to acidic lipid membranes. Prothrombin and prothrombin fragment 1 were examined under four different conditions: in the presence of (a) Na2EDTA, (b) 5...

journal_title：Biophysical journal

authors： Wu JR,Lentz BR

更新日期：1991-07-01 00:00:00

abstract：:Molecular dynamics simulations of the tripeptide Ala-Phe-Ala-O-tert-butyl interacting with dimyristoylphosphatidylcholine lipid bilayers have been carried out. The lipid and aqueous environments of the peptide, the alkyl chain order, and the lipid and peptide dynamics have been investigated with use of density profile...

journal_title：Biophysical journal

authors： Damodaran KV,Merz KM Jr,Gaber BP

更新日期：1995-10-01 00:00:00

abstract：:The theory of the electromagnetic blood flow measuring technique is extended from the well known case (conductive liquid flowing through an insulating tube) to more realistic situations. First the conductivity of the vessel is taken into account, and the electric potentials in both liquid and vessel wall are calculate...

journal_title：Biophysical journal

authors： GESSNER U

更新日期：1961-11-01 00:00:00

abstract：:The mechanical properties of cross-linked microtubule bundles were measured from outer pillar cells isolated from the mammalian inner ear. Measurements were made using a three-point bending test and were incorporated into a mathematical model designed to distinguish between the stiffness contributions from microtubule...

journal_title：Biophysical journal

authors： Tolomeo JA,Holley MC

更新日期：1997-10-01 00:00:00

abstract：:Alkaline sucrose sedimentation studies of DNA from mouse lymphoma cells (L5178Y) treated with caffeine have demonstrated the following effects. Caffeine (at a concentration of 1.6 mM) does not introduce strand breaks into preformed DNA nor does it inhibit the rejoining of gamma-ray-induced strand breaks. Although it d...

journal_title：Biophysical journal

authors： Lehmann AR

更新日期：1972-10-01 00:00:00

abstract：:An analytical electromechanical model of a spherical cell exposed to an alternating electric field was used to calculate shear stress generated in the cellular membrane. Shape deformation of Neurospora crassa (slime) spheroplasts was measured. Statistical analysis permitted empirical evaluation of creep of the cellula...

journal_title：Biophysical journal

authors： Poznański J,Pawłowski P,Fikus M

更新日期：1992-03-01 00:00:00

abstract：:Nonphotochemical quenching is the protective mechanism against overexcitation of photosystem II, triggered by excess ΔpH in photosynthetic membranes. The light-harvesting complexes (LHCs), the de-epoxidation of violaxanthin to zeaxanthin, and the photosystem II subunit S (PsbS) work in synergy for an optimized multile...

journal_title：Biophysical journal

authors： Daskalakis V,Papadatos S

更新日期：2017-12-05 00:00:00

abstract：:In diseases of the brain, the distribution and properties of ion channels display deviations from healthy control subjects. We studied three cases of ion channel alteration related to epileptogenesis. The first case of ion channel alteration represents an enhanced sodium current, the second case addresses the downregu...

journal_title：Biophysical journal

authors： Tigerholm J,Fransén E

更新日期：2011-10-19 00:00:00

abstract：:Crawling cells have characteristic shapes that are a function of their cell types. How their different shapes are determined is an interesting question. Fish epithelial keratocytes are an ideal material for investigating cell shape determination, because they maintain a nearly constant fan shape during their crawling ...

journal_title：Biophysical journal

authors： Nakata T,Okimura C,Mizuno T,Iwadate Y

更新日期：2016-01-19 00:00:00

abstract：:Intracellular passive Ca2+, buffering was measured in voltage-clamped rat ventricular myocytes. Cells were loaded with indo-1 (K+ salt) to an estimated cytosolic concentration of 44 +/- 5 microM (Mean +/- SEM, n = 5), and accessible cell volume was estimated to be 24.5 +/- 3.6 pl. Ca2+ transport by the sarcoplasmic re...

journal_title：Biophysical journal

authors： Berlin JR,Bassani JW,Bers DM

更新日期：1994-10-01 00:00:00

abstract：:In rat mesenteric arteries, smooth muscle cells exhibit intercellular calcium waves in response to local phenylephrine stimulation. These waves have a velocity of approximately 20 cells/s and a range of approximately 80 cells. We analyze these waves in a theoretical model of a population of coupled smooth muscle cells...

journal_title：Biophysical journal

authors： Koenigsberger M,Seppey D,Bény JL,Meister JJ

更新日期：2010-07-21 00:00:00

abstract：:We propose a mechanism for the formation of membrane oscillations and traveling waves, which arise due to the coupling between the actin cytoskeleton and the calcium flux through the membrane. In our model, the fluid cell membrane has a mobile but constant population of proteins with a convex spontaneous curvature, wh...

journal_title：Biophysical journal

authors： Veksler A,Gov NS

更新日期：2009-09-16 00:00:00