Protein logic: a statistical mechanical study of signal integration at the single-molecule level.

abstract：:Endophilins are SH3- and BAR domain-containing proteins implicated in membrane remodeling and vesicle formation. Endophilins A1 and A2 promote the budding of endocytic vesicles from the plasma membrane, whereas endophilin B1 has been implicated in vesicle budding from intracellular organelles, including the trans-Golg...

journal_title：Biophysical journal

authors： Li J,Barylko B,Eichorst JP,Mueller JD,Albanesi JP,Chen Y

更新日期：2016-08-09 00:00:00

abstract：:Wiener kernels have been measured for the light growth response of a number of mutants of Phycomyces which show abnormal phototropism (mad mutants). Representative mutants were chosen from the six complementation groups (madA to madF) associated with the light response pathway. One group, madA, associated with the inp...

journal_title：Biophysical journal

authors： Lipson ED

更新日期：1975-10-01 00:00:00

abstract：:To study why pancreatic beta-cells prefer to burst as a multi-cellular complex, we have formulated a stochastic model for bursting clusters of excitable cells. Our model incorporated a delayed rectifier K+ channel, a fast voltage-gated Ca2+ channel, and a slow Cai-blockable Ca2+ channel. The fraction of ATP-sensitive ...

journal_title：Biophysical journal

authors： Chay TR,Kang HS

更新日期：1988-09-01 00:00:00

abstract：:We used the nonlinear Poisson-Boltzmann equation to calculate electrostatic potentials in the aqueous phase adjacent to model phospholipid bilayers containing mixtures of zwitterionic lipids (phosphatidylcholine) and acidic lipids (phosphatidylserine or phosphatidylglycerol). The aqueous phase (relative permittivity, ...

journal_title：Biophysical journal

authors： Peitzsch RM,Eisenberg M,Sharp KA,McLaughlin S

更新日期：1995-03-01 00:00:00

abstract：:Hyaluronan (HA) is a highly hydrated polyanion, which is a network-forming and space-filling component in the extracellular matrix of animal tissues. Confocal fluorescence recovery after photobleaching (confocal-FRAP) was used to investigate intramolecular hydrogen bonding and electrostatic interactions in hyaluronan ...

journal_title：Biophysical journal

authors： Gribbon P,Heng BC,Hardingham TE

更新日期：1999-10-01 00:00:00

abstract：:Three-dimensional structures were determined for three crystal forms of the antigen binding fragment (Fab) of anti-fluorescein antibody 4-4-20 in complex with fluorescein. These included 1) a triclinic (P1) form crystallized in 47% (v/v) 2-methyl-2,4-pentanediol (MPD); 2) a triclinic (P1) form crystallized in 16% (w/v...

journal_title：Biophysical journal

authors： Herron JN,Terry AH,Johnston S,He XM,Guddat LW,Voss EW Jr,Edmundson AB

更新日期：1994-12-01 00:00:00

abstract：:The electrophoretic behavior of defined DNA and RNA oligonucleotide duplexes from 10 to 20 bp in length has been investigated as a function of salt conditions, gel concentration, and temperature. The RNA oligomers migrated much more slowly than the DNA oligomers of the same sequence under all conditions. From sediment...

journal_title：Biophysical journal

authors： Bonifacio GF,Brown T,Conn GL,Lane AN

更新日期：1997-09-01 00:00:00

abstract：:Molecular sensors based on intramolecular Förster resonance energy transfer (FRET) have become versatile tools to monitor regulatory molecules in living tissue. However, their use is often compromised by low signal strength and excessive noise. We analyzed signal/noise (SNR) aspects of spectral FRET analysis methods, ...

journal_title：Biophysical journal

authors： Woehler A,Wlodarczyk J,Neher E

更新日期：2010-10-06 00:00:00

abstract：:A simple method of generating electric field-induced concentration gradients in planar supported bilayers has been developed. Gradients of charged, fluorescently labeled probes were visualized by epifluorescence microscopy and could be observed at field strengths as low as 1 V/cm. Steady-state concentration gradients ...

journal_title：Biophysical journal

authors： Groves JT,Boxer SG

更新日期：1995-11-01 00:00:00

abstract：:The photoelectron quantum yield spectrum of bacteriochlorophyll aGg (Bchl a ) from Rhodospirillum rubrum was determined in order to evaluate the possibility of mapping photoreceptor distribution and organization in bacterial chromatophores. The quantum yield is on the order of 1 X 10(-3) electrons/incident photon at 1...

journal_title：Biophysical journal

authors： Barnes RB,Amend J,Sistrom WR,Griffith OH

更新日期：1978-03-01 00:00:00

abstract：:Concentrated solutions of bovine beta-lactoglobulin were studied using osmotic stress and rheological techniques. At pH 6.0 and 8.0, the osmotic pressure was largely independent of NaCl concentration and could be described by a hard sphere equation of state. At pH 5.1, close to the isoelectric point, the osmotic press...

journal_title：Biophysical journal

authors： Parker R,Noel TR,Brownsey GJ,Laos K,Ring SG

更新日期：2005-08-01 00:00:00

abstract：:The goal of this paper is to examine the origins and relative importance of primary and secondary sources of electric and magnetic fields for excitable tissue. It is shown that for axonal and cardiac tissue a comparison of the relative field strength from both primary and secondary sources shows only the latter to be ...

journal_title：Biophysical journal

authors： Plonsey R

更新日期：1982-09-01 00:00:00

abstract：:The barbiturate pentobarbital binds to gamma-aminobutyric acid type A (GABA(A)) receptors, and this interaction plays an important role in the anesthetic action of this drug. Depending on its concentration, pentobarbital can potentiate (approximately 10-100 microM), activate (approximately 100-800 microM), or block (a...

journal_title：Biophysical journal

authors： Muroi Y,Theusch CM,Czajkowski C,Jackson MB

更新日期：2009-01-01 00:00:00

abstract：:The diffusive motion of DNA-containing chromatin in live cells and isolated nuclei is investigated using a two-photon standing wave fluorescence photobleaching experiment with 100 nm spatial resolution. The chromatin is labeled using the minor groove binding dye Hoechst 33342. In live cells, the mean diffusion rate is...

journal_title：Biophysical journal

authors： Davis SK,Bardeen CJ

更新日期：2004-01-01 00:00:00

abstract：:The polar interface of membranes containing phosphatidylglycerol or cholesterol was studied by (2)H nuclear magnetic resonance (NMR) as a function of membrane hydration. The membranes were macroscopically aligned and hydrated with deuterium oxide. Water uptake and membrane annealing was achieved under NMR control, usi...

journal_title：Biophysical journal

authors： Kurze V,Steinbauer B,Huber T,Beyer K

更新日期：2000-05-01 00:00:00

abstract：:Human posttranslationally modified N-ras oncogenes are known to be implicated in numerous human cancers. Here, we applied a combination of experimental and computational techniques to determine structural and dynamical details of the lipid chain modifications of an N-ras heptapeptide in 1,2-dimyristoyl-sn-glycero-3-ph...

journal_title：Biophysical journal

authors： Vogel A,Tan KT,Waldmann H,Feller SE,Brown MF,Huster D

更新日期：2007-10-15 00:00:00

abstract：:The role of desolvation in protein binding kinetics is investigated using Brownian dynamics simulations in complexes in which the electrostatic interactions are relatively weak. We find that partial desolvation, modeled by a short-range atomic contact potential, is not only a major contributor to the binding free ener...

journal_title：Biophysical journal

authors： Camacho CJ,Kimura SR,DeLisi C,Vajda S

更新日期：2000-03-01 00:00:00

abstract：:In the first issue, on the first page of the Biophysical Journal in 1960, Cole and Moore provided the first confirmation of the Hodgkin and Huxley formulation of the sodium and potassium conductances that underlie the action potential. In addition, working with the squid giant axon, Cole and Moore noted that strong hy...

journal_title：Biophysical journal

authors： Hoshi T,Armstrong CM

更新日期：2015-10-06 00:00:00

abstract：:The results of variable dielectric coefficient Poisson-Boltzmann calculations of the counter-ion concentration in the vicinity of an all-atom model of the B-form of DNA are presented with an emphasis on the importance of spatial variations in the dielectric properties of the solvent, particularly at the macro-ion-solv...

journal_title：Biophysical journal

authors： Pack GR,Garrett GA,Wong L,Lamm G

更新日期：1993-10-01 00:00:00

abstract：:We describe a model that relates the maximum shortening velocity of a muscle fiber, Vm, to the kinetics of the dissociation of a myosin head from actin. At Vm, the positive work exerted by cross-bridges attached in the powerstroke must be balanced by cross-bridges that have been carried by movement of the filaments in...

journal_title：Biophysical journal

authors： Cooke R,White H,Pate E

更新日期：1994-03-01 00:00:00

abstract：:In voltage-clamped frog skeletal muscle fibers, Ca(2+) release rises rapidly to a peak, then decays to a nearly steady state. The voltage dependence of the ratio of amplitudes of these two phases (p/s) shows a maximum at low voltages and declines with further depolarization. The peak phase has been attributed to a com...

journal_title：Biophysical journal

authors： Brum G,Piriz N,DeArmas R,Rios E,Stern M,Pizarro G

更新日期：2003-07-01 00:00:00

abstract：:With a view to determine the configuration and regularity of plectonemically supercoiled DNA, we have measured the small angle neutron scattering from pUC18 plasmid in saline solutions. Furthermore, we have derived the mathematical expression for the single chain scattering function (form factor) of a superhelical str...

journal_title：Biophysical journal

authors： Zakharova SS,Jesse W,Backendorf C,Egelhaaf SU,Lapp A,van der Maarel JR

更新日期：2002-08-01 00:00:00

abstract：:We have studied the kinetics of N epsilon-fluorescein isothiocyanate-lysine-23 cobra alpha-toxin (FITC-toxin) binding to the membrane-associated acetylcholine receptor from the Torpedo californica electric organ. The fluorescent toxin not only enabled us to monitor the binding reaction continuously but also to examine...

journal_title：Biophysical journal

authors： Cheung AT,Johnson DA,Taylor P

更新日期：1984-02-01 00:00:00

abstract：:The endoplasmic reticulum (ER) in plant cells forms a highly dynamic network of complex geometry. ER network morphology and dynamics are influenced by a number of biophysical processes, including filament/tubule tension, viscous forces, Brownian diffusion, and interactions with many other organelles and cytoskeletal e...

journal_title：Biophysical journal

authors： Lin C,White RR,Sparkes I,Ashwin P

更新日期：2017-07-11 00:00:00

abstract：:Unsaturated phospholipid is the membrane component that is essential to the dynamic environment needed for biomembrane function. The dependence of the chain-melting transition temperature, T(t), of phospholipid bilayer membranes on the position, n(u), of the cis double bond in the glycerophospholipid sn-2 chain can be...

journal_title：Biophysical journal

authors： Marsh D

更新日期：1999-08-01 00:00:00

abstract：:We describe the use of asymmetric Ru-ligand complexes as a new class of luminescent probes that can be used to measure rotational motions of proteins. These complexes are known to display luminescent lifetimes ranging from 10 to 4000 ns. In this report, we show that the asymmetric complex Ru(bpy)2(dcbpy) (PF6)2 displa...

journal_title：Biophysical journal

authors： Terpetschnig E,Szmacinski H,Malak H,Lakowicz JR

更新日期：1995-01-01 00:00:00

abstract：:Glucagon solutions at pH 2.0 were subjected to mechanical agitation at 37 degrees C in the presence of a hydrophobic surface to explore the details of aggregation and fiber formation. High-resolution intermittent-contact atomic force microscopy performed in solution revealed the presence of aggregates after 0.5 h; how...

journal_title：Biophysical journal

authors： De Jong KL,Incledon B,Yip CM,DeFelippis MR

更新日期：2006-09-01 00:00:00

abstract：:Phosphatidylinositol-4,5-bisphosphate (PIP2) is an important signaling lipid in eukaryotic cell plasma membranes, playing an essential role in diverse cellular processes. The headgroup of PIP2 is highly negatively charged, and this lipid displays a high critical micellar concentration compared to housekeeping phosphol...

journal_title：Biophysical journal

authors： Shukla S,Jin R,Robustelli J,Zimmerman ZE,Baumgart T

更新日期：2019-09-03 00:00:00

abstract：:Methods are described for discrimination of models of the gating kinetics and permeation of single ionic channels. Both maximum likelihood and regression procedures are discussed. In simple situations, where models are nested, standard hypothesis tests can be used. More commonly, however, non-nested models are of inte...

journal_title：Biophysical journal

authors： Horn R

更新日期：1987-02-01 00:00:00

abstract：:To understand better the effect of electrostatics on the rigidity of the DNA double helix, we define DNA*, the null isomer of DNA, as the hypothetical structure that would result from DNA if its phosphate groups were not ionized. For the purposes of theoretical analysis, we model DNA* as identical to ordinary DNA but ...

journal_title：Biophysical journal

authors： Manning GS

更新日期：2006-11-15 00:00:00