Sequential modification of membrane currents with classical conditioning.

abstract：:Limited proteolysis of RNase-A yields a short N-terminal S-peptide segment and the larger S-protein. Binding of S-peptide to S-protein results in the formation of an enzymatically active RNase-S protein. S-peptide undergoes a transition from intrinsic disorder to an ordered helical state upon association with S-protei...

journal_title：Biophysical journal

authors： Luitz MP,Bomblies R,Zacharias M

更新日期：2017-10-03 00:00:00

abstract：:We continue in this paper the presentation of theoretical and experimental methods for the joint refinement of neutron and x-ray lamellar diffraction data for the analysis of fluid (L alpha phase) bilayer structure (Wiener, M. C., and S. H. White. 1991 a, b, c. Biophys. J. 59:162-173 and 174-185; Biochemistry. 30:6997...

journal_title：Biophysical journal

authors： Wiener MC,White SH

更新日期：1992-02-01 00:00:00

abstract：:The plasma membrane (PM) serves as the functional interface between a cell and its environment, hosting extracellular signal transduction and nutrient transport among a variety of other processes. To support this extensive functionality, PMs are organized into lateral domains, including ordered, lipid-driven assemblie...

journal_title：Biophysical journal

authors： Levental KR,Lorent JH,Lin X,Skinkle AD,Surma MA,Stockenbojer EA,Gorfe AA,Levental I

更新日期：2016-04-26 00:00:00

abstract：:Efficient and rapid immune response upon challenge by an infectious agent is vital to host defense. The encounter of leukocytes (white blood cells of the immune system) with their targets is the first step in this response. Analysis of the kinetics of this process is essential not only to understanding dynamic behavio...

journal_title：Biophysical journal

authors： Charnick SB,Lauffenburger DA

更新日期：1990-05-01 00:00:00

abstract：:Monolayers of a functional pulmonary surfactant (PS) can reach very low surface tensions well below their equilibrium value. The mechanism by which PS monolayers reach such low surface tensions and maintain film stability remains unknown. As shown previously by fluorescence microscopy, phospholipid phase transition an...

journal_title：Biophysical journal

authors： Zuo YY,Keating E,Zhao L,Tadayyon SM,Veldhuizen RA,Petersen NO,Possmayer F

更新日期：2008-05-01 00:00:00

abstract：:The effects of lipid chain packing and permeant size and shape on permeability across lipid bilayers have been investigated in gel and liquid crystalline dipalmitoylphosphatidylcholine (DPPC) bilayers by a combined NMR line-broadening/dynamic light scattering method using seven short-chain monocarboxylic acids (formic...

journal_title：Biophysical journal

authors： Xiang TX,Anderson BD

更新日期：1998-12-01 00:00:00

abstract：:The distribution of a selection of cationic fluorescent dyes can be used to measure the membrane potential of individual cells with a microfluorometer. The essential attributes of these dyes include membrane permeability, low membrane binding, spectral properties which are insensitive to environment, and, of course, s...

journal_title：Biophysical journal

authors： Ehrenberg B,Montana V,Wei MD,Wuskell JP,Loew LM

更新日期：1988-05-01 00:00:00

abstract：:Hydrophobic interactions between a bilayer and its embedded membrane proteins couple protein conformational changes to changes in the packing of the surrounding lipids. The energetic cost of a protein conformational change therefore includes a contribution from the associated bilayer deformation energy (DeltaGdef0), w...

journal_title：Biophysical journal

authors： Lundbaek JA,Andersen OS

更新日期：1999-02-01 00:00:00

abstract：:Ion channels enable diffusion of ions down physiological electrochemical gradients. Modulation of ion permeation is crucial for the physiological functioning of cells, and misregulation of ion channels is linked to a myriad of channelopathies. The ion permeation mechanism in the transient receptor potential (TRP) ion ...

journal_title：Biophysical journal

authors： Jorgensen C,Furini S,Domene C

更新日期：2016-09-20 00:00:00

abstract：:Numerous membrane-transport proteins are major drug targets, and therefore a key ingredient in pharmaceutical development is the availability of reliable, efficient tools for membrane transport characterization and inhibition. Here, we present the use of evanescent-wave sensing for screening of membrane-protein-mediat...

journal_title：Biophysical journal

authors： Brändén M,Tabaei SR,Fischer G,Neutze R,Höök F

更新日期：2010-07-07 00:00:00

abstract：:We have used fluorescence-quenching measurements to characterize the partitioning of a variety of indolyl-labeled phospho- and sphingolipids between gel or liquid-ordered and liquid-disordered lipid domains in several types of lipid bilayers where such domains coexist. In both cholesterol-free and cholesterol-containi...

journal_title：Biophysical journal

authors： Wang TY,Silvius JR

更新日期：2003-01-01 00:00:00

abstract：:The kinetics of aggregation of alpha-synuclein are usually studied by turbidity or Thio-T fluorescence. Here we follow the disappearance of monomers and the formation of early oligomers using fluorescence correlation spectroscopy. Alexa488-labeled A140C-synuclein was used as a fluorescent probe in trace amounts in the...

journal_title：Biophysical journal

authors： Nath S,Meuvis J,Hendrix J,Carl SA,Engelborghs Y

更新日期：2010-04-07 00:00:00

abstract：:Bovine brain phosphatidylserine (BBPS) vesicles were prepared with traces of dioleoylglycerol (18:1, 18:1 DAG) or hexadecane (HD) to determine the influence of changes in headgroup or acyl chain packing on divalent cation-induced lipid mixing rates. A stopped-flow apparatus was used to combine vesicles with 3 mM Ca2+ ...

journal_title：Biophysical journal

authors： Walter A,Yeagle PL,Siegel DP

更新日期：1994-02-01 00:00:00

abstract：:We study the effect of ergosterol on the physical properties of 1-[(2)H(31)]palmitoyl-2-oleoyl-sn-glycero-3-phosphoethanolamine (POPE) multibilayers using deuterium nuclear magnetic resonance. NMR spectra were taken as a function of temperature and ergosterol concentration up to 70 mol %. The spectral first moments sh...

journal_title：Biophysical journal

authors： Hsueh YW,Weng CJ,Chen MT,Thewalt J,Zuckermann M

更新日期：2010-04-07 00:00:00

abstract：:We have identified two kinetically distinct modes of block, by lidocaine, of cardiac sodium channels, activated by batrachotoxin and incorporated into planar lipid bilayers. Here, we analyze the slow blocking mode which appears as a series of nonconducting events that increase in frequency and duration with increasing...

journal_title：Biophysical journal

authors： Zamponi GW,Doyle DD,French RJ

更新日期：1993-07-01 00:00:00

abstract：:The structural changes in the photoreaction cycle of bacteriorhodopsin, a light-driven proton pump, was investigated at a resolution of 7 angstroms by a time-resolved x-ray diffraction experiment utilizing synchrotron x rays from an undulator of SPring-8. The x-ray diffraction measurement system, used in coupling with...

journal_title：Biophysical journal

authors： Oka T,Inoue K,Kataoka M,Yagi N

更新日期：2005-01-01 00:00:00

abstract：:A pulsed source neutron spectrometer has been used to measure vibrational spectra (20-4000 cm-1) of dry and hydrated type I collagen fibers, and of two model polypeptides, polyproline II and (prolyl-prolyl-glycine)10, at temperatures of 30 and 120 K. the collagen spectra provide the first high resolution neutron views...

journal_title：Biophysical journal

authors： Middendorf HD,Hayward RL,Parker SF,Bradshaw J,Miller A

更新日期：1995-08-01 00:00:00

abstract：:Because of their remarkable ability to depress the freezing point of aqueous solutions, antifreeze proteins (AFPs) play a critical role in helping many organisms survive subzero temperatures. The beta-helical insect AFP structures solved to date, consisting of multiple repeating circular loops or coils, are perhaps th...

journal_title：Biophysical journal

authors： Liu K,Jia Z,Chen G,Tung C,Liu R

更新日期：2005-02-01 00:00:00

abstract：:Bistability is a major mechanism for cellular decision making and usually results from positive feedback in biochemical control systems. Here we show theoretically that bistability between unbound and bound states of adhesion clusters results from positive feedback mediated by structural rather than biochemical proces...

journal_title：Biophysical journal

authors： Erdmann T,Schwarz US

更新日期：2006-09-15 00:00:00

abstract：:In the last decades, it has been demonstrated that many animal species orient in the Earth magnetic field. One of the best-studied examples is the use of the geomagnetic field by migratory birds for orientation and navigation. However, the biophysical mechanism underlying animal magnetoreception is still not understoo...

journal_title：Biophysical journal

authors： Solov'yov IA,Schulten K

更新日期：2009-06-17 00:00:00

abstract：:We study the stochastic dynamics of growth and shrinkage of single actin filaments taking into account insertion, removal, and ATP hydrolysis of subunits either according to the vectorial mechanism or to the random mechanism. In a previous work, we developed a model for a single actin or microtubule filament where hyd...

journal_title：Biophysical journal

authors： Ranjith P,Mallick K,Joanny JF,Lacoste D

更新日期：2010-04-21 00:00:00

abstract：:The electrophoretic behavior of defined DNA and RNA oligonucleotide duplexes from 10 to 20 bp in length has been investigated as a function of salt conditions, gel concentration, and temperature. The RNA oligomers migrated much more slowly than the DNA oligomers of the same sequence under all conditions. From sediment...

journal_title：Biophysical journal

authors： Bonifacio GF,Brown T,Conn GL,Lane AN

更新日期：1997-09-01 00:00:00

abstract：:Measurements of transepithelial electrical impedance of continuously short-circuited A6 epithelia were made at audio frequencies (0.244 Hz to 10.45 kHz) to investigate the time course and extent to which prostaglandin E(2) (PGE(2)) modulates Cl(-) transport and apical membrane capacitance in this cell-cultured model e...

journal_title：Biophysical journal

authors： Păunescu TG,Helman SI

更新日期：2001-08-01 00:00:00

abstract：:Direct spectroscopic measurements of rotational motions of proteins and large protein segments are crucial to understanding the molecular dynamics of protein function. Fluorescent probes and spin labels attached to proteins have proved to be powerful tools in the study of large-scale protein motions. Fluorescence depo...

journal_title：Biophysical journal

authors： Thomas DD

更新日期：1978-11-01 00:00:00

abstract：:Recent development of titratable coions has paved the way for realizing all-atom molecular dynamics at constant pH. To further improve physical realism, here we describe a technique in which proton titration of the solute is directly coupled to the interconversion between water and hydroxide or hydronium. We test the ...

journal_title：Biophysical journal

authors： Chen W,Wallace JA,Yue Z,Shen JK

更新日期：2013-08-20 00:00:00

abstract：:An approach is described using fluorescence resonance energy transfer (FRET) to detect inhomogeneity in lipid organization, on distance scales of the order of tens of nanometers or greater, in lipid bilayers. This approach compares the efficiency of energy transfer between two matched fluorescent lipid donors, differi...

journal_title：Biophysical journal

authors： Silvius JR

更新日期：2003-08-01 00:00:00

abstract：:We present elastic and inelastic incoherent neutron scattering data from a series of trehalose glasses diluted with glycerol. A strong correlation with recently published protein stability data in the same series of glasses illustrates that the dynamics at Q >or= 0.71 A(-1) and omega > 200 MHz are important to stabili...

journal_title：Biophysical journal

authors： Cicerone MT,Soles CL

更新日期：2004-06-01 00:00:00

abstract：:A triple mutant of sperm whale myoglobin (Mb) [Leu(B10) --> Tyr, His(E7) --> Gln, and Thr(E10) --> Arg, called Mb-YQR], investigated by stopped-flow, laser photolysis, crystallography, and molecular dynamics (MD) simulations, proved to be quite unusual. Rebinding of photodissociated NO, O2, and CO from within the prot...

journal_title：Biophysical journal

authors： Brunori M,Cutruzzolà F,Savino C,Travaglini-Allocatelli C,Vallone B,Gibson QH

更新日期：1999-03-01 00:00:00

abstract：:The cytoskeletal activity of motile or adherent cells is frequently seen to induce detectable displacements of sufficiently compliant substrata. The physics of this phenomenon is discussed in terms of the classical theory of small-strain, plane-stress elasticity. The main results of such analysis is a transform expres...

journal_title：Biophysical journal

authors： Dembo M,Oliver T,Ishihara A,Jacobson K

更新日期：1996-04-01 00:00:00

abstract：:Steady-state and time-resolved fluorescence spectroscopy was used to follow the local and global changes in structure and dynamics during chemical and thermal denaturation of unlabeled human serum albumin (HSA) and HSA with an acrylodan moiety bound to Cys34. Acrylodan fluorescence was monitored to obtain information ...

journal_title：Biophysical journal

authors： Flora K,Brennan JD,Baker GA,Doody MA,Bright FV

更新日期：1998-08-01 00:00:00