Vortex shedding as a precursor of turbulent electrical activity in cardiac muscle.

abstract：:The detection of the 1H NMR signal of myoglobin (Mb) in tissue opens an opportunity to examine its cellular diffusion property, which is central to its purported role in facilitating oxygen transport. In perfused myocardium the field-dependent transverse relaxation analysis of the deoxy Mb proximal histidyl NdeltaH in...

journal_title：Biophysical journal

authors： Wang D,Kreutzer U,Chung Y,Jue T

更新日期：1997-11-01 00:00:00

abstract：:Subdenaturing concentrations of guanidine hydrochloride (GdnHCl) stabilize proteins. For ferrocytochrome c the stabilization is detected at subglobal level with no measured change in global stability. These deductions are made by comparing observed rates of thermally driven ferrocytochrome cHCO reactions with global u...

journal_title：Biophysical journal

authors： Kumar R,Prabhu NP,Yadaiah M,Bhuyan AK

更新日期：2004-10-01 00:00:00

abstract：:A detailed experimental description of molecular dynamics requires an accurate description of the global correlation time. For the gramicidin cation-selective channel the local dynamics of the polypeptide backbone are thought to play a very significant role in the functional process of this channel which occurs on the...

journal_title：Biophysical journal

authors： Lee KC,Hu W,Cross TA

更新日期：1993-09-01 00:00:00

abstract：:Chlamydomonas exhibits a sequence of a photoreceptor current and two flagellar currents upon stimulation with bright green flashes. The currents are thought to be a prerequisite for the well-known photophobic responses. In the preceding paper, we analyzed the kinetics of these currents and their dependence on extracel...

journal_title：Biophysical journal

authors： Nonnengässer C,Holland EM,Harz H,Hegemann P

更新日期：1996-02-01 00:00:00

abstract：:One of the disadvantages of the sucrose-gap method for measuring membrane potentials with extracellular electrodes is a membrane hyperpolarization of the order of 30 to 60 mv, as compared with the resting potential obtained with intracellular microelectrodes in the absence of a sucrose-gap. In the present study the co...

journal_title：Biophysical journal

authors： Blaustein MP,Goldman DE

更新日期：1966-07-01 00:00:00

abstract：:Laurdan fluorescence, novel spectral fitting, and dynamic light scattering were combined to determine lateral lipid organization in mixed lipid membranes of the oxidized lipid, 1-palmitoyl-2-glutaryl-sn-glycero-3-phosphocholine (PGPC), and each of the three bilayer lipids, 1,2-dipalmitoyl-sn-glycero-3-phosphocholine (...

journal_title：Biophysical journal

authors： Ranganathan R,Alshammri I,Peric M

更新日期：2020-04-21 00:00:00

abstract：:Fibrin polymerizes to produce branching fibers forming a three-dimensional network, which has been difficult to visualize by conventional microscopy. Three-dimensional images of whole clots at high resolution were obtained from stereo-pair intermediate-voltage electron micrographs. Computer software was developed to p...

journal_title：Biophysical journal

authors： Baradet TC,Haselgrove JC,Weisel JW

更新日期：1995-04-01 00:00:00

abstract：:Phenotypic plasticity is posed to be a vital trait of cancer cells such as circulating tumor cells, allowing them to undergo reversible or irreversible switching between phenotypic states important for tumorigenesis and metastasis. While irreversible phenotypic switching can be detected by studying the genome, reversi...

journal_title：Biophysical journal

authors： Aw Yong KM,Sun Y,Merajver SD,Fu J

更新日期：2017-03-28 00:00:00

abstract：:We present a single virion method to determine absolute distributions of copy number in the protein composition of viruses and apply it to herpes simplex virus type 1. Using two-color coincidence fluorescence spectroscopy, we determine the virion-to-virion variability in copy numbers of fluorescently labeled tegument ...

journal_title：Biophysical journal

authors： Clarke RW,Monnier N,Li H,Zhou D,Browne H,Klenerman D

更新日期：2007-08-15 00:00:00

abstract：:The conformation of single laminin molecules adsorbed on synthetic substrates is directly observed making use of the phase magnitude in tapping mode atomic force microscopy (AFM). With AFM, it is not possible to differentiate the proteins on the substrate if use is made of the height signal, since the roughness of the...

journal_title：Biophysical journal

authors： Rodríguez Hernández JC,Salmerón Sánchez M,Soria JM,Gómez Ribelles JL,Monleón Pradas M

更新日期：2007-07-01 00:00:00

abstract：:Structures of truncated versions of the influenza A virus M2 proton channel have been determined recently by x-ray crystallography in the open conformation of the channel, and by NMR in the closed state. The structures differ in the position of the bound inhibitors. The x-ray structure shows a single amantadine molecu...

journal_title：Biophysical journal

authors： Chuang GY,Kozakov D,Brenke R,Beglov D,Guarnieri F,Vajda S

更新日期：2009-11-18 00:00:00

abstract：:The last decade established that the dynamic properties of the phosphoproteome are central to function and its modulation. The temporal dimension of phosphorylation effects remains nonetheless poorly understood, particularly for intrinsically disordered proteins. Osteopontin, selected for this study due to its key rol...

journal_title：Biophysical journal

authors： Lenton S,Grimaldo M,Roosen-Runge F,Schreiber F,Nylander T,Clegg R,Holt C,Härtlein M,García Sakai V,Seydel T,Marujo Teixeira SC

更新日期：2017-04-25 00:00:00

abstract：:We measured the viscoelastic properties of the cytoplasm of J774 macrophages with a recently developed microrheometer. Ferromagnetic beads (1.3 microm in diameter) were used to determine the local viscoelastic moduli. Step-force pulses were applied to the magnetic beads and the displacement was observed by single part...

journal_title：Biophysical journal

authors： Bausch AR,Möller W,Sackmann E

更新日期：1999-01-01 00:00:00

abstract：:Pure Michaelis-Menten enzymes have been studied (i.e., enzymes with a hyperbolic (S, V) behavior in a well-stirred solution). When such enzymes are associated with a structure in vitro, even in the simplest conceivable form (immobilization in a homogeneous gel), they can produce enzymic or transport reactions with man...

journal_title：Biophysical journal

authors： Vincent JC,Thellier M

更新日期：1983-01-01 00:00:00

abstract：:Fluorescence fluctuation spectroscopy has become an important measurement tool for investigating molecular dynamics, molecular interactions, and chemical kinetics in biological systems. Although the basic theory of fluctuation spectroscopy is well established, it is not widely recognized that saturation of the fluores...

journal_title：Biophysical journal

authors： Nagy A,Wu J,Berland KM

更新日期：2005-09-01 00:00:00

abstract：:The kinetic properties of the three taxonomic A substates of sperm whale carbonmonoxy myoglobin in 75% glycerol/buffer are studied by flash photolysis with monitoring in the infrared stretch bands of bound CO at nu(A0) approximately 1967 cm-1, nu(A1) approximately 1947 cm-1, and nu(A3) approximately 1929 cm-1 between ...

journal_title：Biophysical journal

authors： Johnson JB,Lamb DC,Frauenfelder H,Müller JD,McMahon B,Nienhaus GU,Young RD

更新日期：1996-09-01 00:00:00

abstract：:The mechanism of leakage induced by surface active peptides is not yet fully understood. To gain insight into the molecular events underlying this process, the leakage induced by the peptide pardaxin from phosphatidylcholine/ phosphatidylserine/cholesterol large unilamellar vesicles was studied by monitoring the rate ...

journal_title：Biophysical journal

authors： Rapaport D,Peled R,Nir S,Shai Y

更新日期：1996-06-01 00:00:00

abstract：:The extracellular matrix contains components with remarkable mechanical properties, including fibronectin (Fn) fibers with extensibilities of >700% strain. We utilized what we consider a novel technique to quantify the extent of molecular unfolding that contributes to Fn fiber extension, and we compared this behavior ...

journal_title：Biophysical journal

authors： Bradshaw MJ,Smith ML

更新日期：2011-10-05 00:00:00

abstract：:We examined the effect of temperature (12-40°C) on local and global Ca2+ signals mediated by inositol trisphosphate receptor/channels (IP3R) in human neuroblastoma (SH-SY5Y) cells. The amplitudes and spatial spread of local signals arising from single IP3R (blips) and clusters of IP3R (puffs) showed little temperature...

journal_title：Biophysical journal

authors： Dickinson GD,Parker I

更新日期：2013-01-22 00:00:00

abstract：:Nef is an HIV-1 accessory protein that directly contributes to AIDS progression. Nef is myristoylated on the N-terminus, associates with membranes, and may undergo a transition from a solution conformation to a membrane-associated conformation. It has been hypothesized that conformational rearrangement enables membran...

journal_title：Biophysical journal

authors： Kent MS,Murton JK,Sasaki DY,Satija S,Akgun B,Nanda H,Curtis JE,Majewski J,Morgan CR,Engen JR

更新日期：2010-09-22 00:00:00

abstract：:An understanding of the structure-dynamics relationship is essential for understanding how a protein works. Prior research has shown that the activity of a protein correlates with intradomain dynamics occurring at picosecond to millisecond timescales. However, the correlation between interdomain dynamics and the funct...

journal_title：Biophysical journal

authors： Kumar L,Nash A,Harms C,Planas-Iglesias J,Wright D,Klein-Seetharaman J,Sarkar SK

更新日期：2020-07-21 00:00:00

abstract：:We use fluorescence correlation spectroscopy and fluorescence recovery after photobleaching to study vesicle dynamics inside the synapses of cultured hippocampal neurons labeled with the fluorescent vesicle marker FM 1-43. These studies show that when the cell is electrically at rest, only a small population of vesicl...

journal_title：Biophysical journal

authors： Shtrahman M,Yeung C,Nauen DW,Bi GQ,Wu XL

更新日期：2005-11-01 00:00:00

abstract：:This work describes the first cell-based model of tumor-induced angiogenesis. At the extracellular level, the model describes diffusion, uptake, and decay of tumor-secreted pro-angiogenic factor. At the cellular level, the model uses the cellular Potts model based on system-energy reduction to describe endothelial cel...

journal_title：Biophysical journal

authors： Bauer AL,Jackson TL,Jiang Y

更新日期：2007-05-01 00:00:00

abstract：:Reconstruction of genome-scale metabolic networks is now possible using multiple different data types. Constraint-based modeling is an approach to interrogate capabilities of reconstructed networks by constraining possible cellular behavior through the imposition of physicochemical laws. As a result, a steady-state fl...

journal_title：Biophysical journal

authors： Price ND,Schellenberger J,Palsson BO

更新日期：2004-10-01 00:00:00

abstract：:Green flagellates possess rhodopsin-like photoreceptors involved in control of their behavior via generation of photocurrents across the plasma membrane. Chlamydomonas mutants blocked in retinal biosynthesis are "blind," but they can be rescued by the addition of exogenous retinoids. Photosignaling by chlamyrhodopsin ...

journal_title：Biophysical journal

authors： Govorunova EG,Sineshchekov OA,Gärtner W,Chunaev AS,Hegemann P

更新日期：2001-11-01 00:00:00

abstract：:Recent studies suggest that phosphoinositide kinases may participate in intracellular trafficking or exocytotic events. Because both of these events ultimately require fusion of biological membranes, the susceptibility of membranes containing polyphosphoinositides (PPIs) to divalent cation-induced fusion was investiga...

journal_title：Biophysical journal

authors： Summers SA,Guebert BA,Shanahan MF

更新日期：1996-12-01 00:00:00

abstract：:Using polarography and suitable mathematical analysis, the oxygen permeability of an individual layer of a bilayered tissue may be determined if the oxygen consumption and permeability of the other layer are known. Results obtained on rabbit cornea indicate that the permeability of the epithelium is about four times t...

journal_title：Biophysical journal

authors： Freeman RD,Fatt I

更新日期：1972-03-01 00:00:00

abstract：:Employing nonequilibrium molecular dynamics simulations, a comprehensive computational study of the photoinduced conformational dynamics of a photoswitchable bicyclic azobenzene octapeptide is presented. The calculation of time-dependent probability distributions along various global and local reaction coordinates rev...

journal_title：Biophysical journal

authors： Nguyen PH,Gorbunov RD,Stock G

更新日期：2006-08-15 00:00:00

abstract：:X-ray diffraction has been applied in measuring the helical pitch of the gramicidin channel in oriented bilayers of dilauroylphosphatidylcholine (DLPC) and dimyristoylphosphatidylcholine (DMPC) at a polypeptide concentration of 9.1 mol %. The diffraction data show the helical pitch of gramicidin to be 4.7 +/- 0.2 A in...

journal_title：Biophysical journal

authors： Katsaras J,Prosser RS,Stinson RH,Davis JH

更新日期：1992-03-01 00:00:00

abstract：:Mislocalization and aggregation of the huntingtin protein are related to Huntington's disease. Its first exon-more specifically the first 17 amino acids (Htt17)-is crucial for the physiological and pathological functions of huntingtin. It regulates huntingtin's activity through posttranslational modifications and serv...

journal_title：Biophysical journal

authors： Côté S,Binette V,Salnikov ES,Bechinger B,Mousseau N

更新日期：2015-03-10 00:00:00