Abstract:
:Subdenaturing concentrations of guanidine hydrochloride (GdnHCl) stabilize proteins. For ferrocytochrome c the stabilization is detected at subglobal level with no measured change in global stability. These deductions are made by comparing observed rates of thermally driven ferrocytochrome cHCO reactions with global unfolding rates of ferrocytochrome c measured by stopped flow and NMR hydrogen exchange in the presence of a wide range of GdnHCl concentrations at pH 7, 22 degrees C.
journal_name
Biophys Jjournal_title
Biophysical journalauthors
Kumar R,Prabhu NP,Yadaiah M,Bhuyan AKdoi
10.1529/biophysj.104.044701subject
Has Abstractpub_date
2004-10-01 00:00:00pages
2656-62issue
4eissn
0006-3495issn
1542-0086pii
S0006-3495(04)73736-4journal_volume
87pub_type
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