Probing vesicle dynamics in single hippocampal synapses.


:We use fluorescence correlation spectroscopy and fluorescence recovery after photobleaching to study vesicle dynamics inside the synapses of cultured hippocampal neurons labeled with the fluorescent vesicle marker FM 1-43. These studies show that when the cell is electrically at rest, only a small population of vesicles is mobile, taking seconds to traverse the synapse. Applying the phosphatase inhibitor okadaic acid causes vesicles to diffuse freely, moving 30 times faster than vesicles in control synapses. These results suggest that vesicles move sluggishly due to binding to elements of the synaptic cytomatrix and that this binding is altered by phosphorylation. Motivated by these results, a model is constructed consisting of diffusing vesicles that bind reversibly to the cytomatrix. This stick-and-diffuse model accounts for the fluorescence correlation spectroscopy and fluorescence recovery after photobleaching data, and also predicts the well-known exponential refilling of the readily releasable pool. Our measurements suggest that the movement of vesicles to the active zone is the rate-limiting step in this process.


Biophys J


Biophysical journal


Shtrahman M,Yeung C,Nauen DW,Bi GQ,Wu XL




Has Abstract


2005-11-01 00:00:00














  • Permeability, phase-boundary potential and conductance in a cholinergic channel without constant field.

    abstract::A potassium-selective, chemically excitable channel, whose characteristics cannot be accurately described by constant-field theory, is studied by a new approach based on diffusion theory but with no need for the classical assumptions of constant field, homogeneous membrane, and equal phase-boundary potentials at both ...

    journal_title:Biophysical journal

    pub_type: 杂志文章


    authors: Schwartz TL,Kado RT

    更新日期:1977-06-01 00:00:00

  • Distributions in protein conformation space: implications for structure prediction and entropy.

    abstract::By considering how polymer structures are distributed in conformation space, we show that it is possible to quantify the difficulty of structural prediction and to provide a measure of progress for prediction calculations. The critical issue is the probability that a conformation is found within a specified distance o...

    journal_title:Biophysical journal

    pub_type: 杂志文章


    authors: Sullivan DC,Kuntz ID

    更新日期:2004-07-01 00:00:00

  • Concentration-jump analysis of voltage-dependent conductances activated by glutamate and kainate in neurons of the avian cochlear nucleus.

    abstract::We have examined the mechanisms underlying the voltage sensitivity of alpha-amino-3-hydroxy-5-methyl-4-isoxazolepropionate receptors in voltage-clamped outside-out patches and whole cells taken from the nucleus magnocellularis of the chick. Responses to either glutamate or kainate had outwardly rectifying current-volt...

    journal_title:Biophysical journal

    pub_type: 杂志文章


    authors: Raman IM,Trussell LO

    更新日期:1995-11-01 00:00:00

  • Analytical calculation of intracellular calcium wave characteristics.

    abstract::We present a theoretical analysis of intracellular calcium waves propagated by calcium feedback at the inositol 1,4,5-trisphosphate (IP3) receptor. The model includes essential features of calcium excitability, but is still analytically tractable. Formulas are derived for the wave speed, amplitude, and width. The calc...

    journal_title:Biophysical journal

    pub_type: 杂志文章


    authors: Kupferman R,Mitra PP,Hohenberg PC,Wang SS

    更新日期:1997-06-01 00:00:00

  • Electrostatic contributions to the stability of a thermophilic cold shock protein.

    abstract::The thermophilic Bacillus caldolyticus cold shock protein (Bc-Csp) differs from the mesophilic Bacillus subtilis cold shock protein B (Bs-CspB) in 11 of the 66 residues. Stability measurements of Schmid and co-workers have implicated contributions of electrostatic interactions to the thermostability. To further elucid...

    journal_title:Biophysical journal

    pub_type: 杂志文章


    authors: Zhou HX,Dong F

    更新日期:2003-04-01 00:00:00

  • Structure of the stacked disk aggregate of tobacco mosaic virus protein.

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    journal_title:Biophysical journal

    pub_type: 杂志文章


    authors: Díaz-Avalos R,Caspar DL

    更新日期:1998-01-01 00:00:00

  • Photochemically induced charge separation occurring in bacteriorhodopsin. Detection by time-resolved dielectric loss.

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    journal_title:Biophysical journal

    pub_type: 杂志文章


    authors: McIntosh AR,Boucher F

    更新日期:1991-07-01 00:00:00

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    journal_title:Biophysical journal

    pub_type: 杂志文章


    authors: Sesti F,Nizzari M,Torre V

    更新日期:1996-06-01 00:00:00

  • Kinetics of microtubule catastrophe assessed by probabilistic analysis.

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    journal_title:Biophysical journal

    pub_type: 杂志文章


    authors: Odde DJ,Cassimeris L,Buettner HM

    更新日期:1995-09-01 00:00:00

  • Modeling Endoplasmic Reticulum Network Maintenance in a Plant Cell.

    abstract::The endoplasmic reticulum (ER) in plant cells forms a highly dynamic network of complex geometry. ER network morphology and dynamics are influenced by a number of biophysical processes, including filament/tubule tension, viscous forces, Brownian diffusion, and interactions with many other organelles and cytoskeletal e...

    journal_title:Biophysical journal

    pub_type: 杂志文章


    authors: Lin C,White RR,Sparkes I,Ashwin P

    更新日期:2017-07-11 00:00:00

  • Secondary structure and lipid contact of a peptide antibiotic in phospholipid bilayers by REDOR.

    abstract::The chemical shifts of specific (13)C and (15)N labels distributed throughout KIAGKIA-KIAGKIA-KIAGKIA (K3), an amphiphilic 21-residue antimicrobial peptide, prove that the peptide is in an all alpha-helical conformation in the bilayers of multilamellar vesicles (MLVs) containing dipalmitoylphosphatidylcholine and dipa...

    journal_title:Biophysical journal

    pub_type: 杂志文章


    authors: Toke O,Maloy WL,Kim SJ,Blazyk J,Schaefer J

    更新日期:2004-07-01 00:00:00

  • Chromophore/protein and chromophore/anion interactions in halorhodopsin.

    abstract::Halorhodopsin (HR), the light-driven chloride transport pigment of Halobacterium halobium, was bleached and reconstituted with retinal analogues with the pi electron system interrupted at different locations (dihydroretinals). The absorption maxima of the artificial pigments formed with the dihydroretinals are found t...

    journal_title:Biophysical journal

    pub_type: 杂志文章


    authors: Lanyi JK,Zimányi L,Nakanishi K,Derguini F,Okabe M,Honig B

    更新日期:1988-02-01 00:00:00

  • Ca2+ regulation of gelsolin activity: binding and severing of F-actin.

    abstract::Regulation of the F-actin severing activity of gelsolin by Ca2+ has been investigated under physiologic ionic conditions. Tryptophan fluorescence intensity measurements indicate that gelsolin contains at least two Ca2+ binding sites with affinities of 2.5 x 10(7) M-1 and 1.5 x 10(5) M-1. At F-actin and gelsolin concen...

    journal_title:Biophysical journal

    pub_type: 杂志文章


    authors: Kinosian HJ,Newman J,Lincoln B,Selden LA,Gershman LC,Estes JE

    更新日期:1998-12-01 00:00:00

  • The effects of ADP and phosphate on the contraction of muscle fibers.

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    journal_title:Biophysical journal

    pub_type: 杂志文章


    authors: Cooke R,Pate E

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  • Role of Inverse-Cone-Shape Lipids in Temperature-Controlled Self-Reproduction of Binary Vesicles.

    abstract::We investigate a temperature-driven recursive division of binary giant unilamellar vesicles (GUVs). During the heating step of the heating-cooling cycle, the spherical mother vesicle deforms to a budded limiting shape using up the excess area produced by the chain melting of the lipids and then splits off into two dau...

    journal_title:Biophysical journal

    pub_type: 杂志文章


    authors: Jimbo T,Sakuma Y,Urakami N,Ziherl P,Imai M

    更新日期:2016-04-12 00:00:00

  • From Gene to Function: Cell-Free Electrophysiological and Optical Analysis of Ion Pumps in Nanodiscs.

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    journal_title:Biophysical journal

    pub_type: 杂志文章


    authors: Henrich E,Sörmann J,Eberhardt P,Peetz O,Mezhyrova J,Morgner N,Fendler K,Dötsch V,Wachtveitl J,Bernhard F,Bamann C

    更新日期:2017-09-19 00:00:00

  • Intermediate and stable redox states of cytochrome c studied by low temperature resonance Raman spectroscopy.

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    journal_title:Biophysical journal

    pub_type: 杂志文章


    authors: Cartling B

    更新日期:1983-08-01 00:00:00

  • Termination of cardiac Ca(2+) sparks: an investigative mathematical model of calcium-induced calcium release.

    abstract::A Ca(2+) spark arises when a cluster of sarcoplasmic reticulum (SR) channels (ryanodine receptors or RyRs) opens to release calcium in a locally regenerative manner. Normally triggered by Ca(2+) influx across the sarcolemmal or transverse tubule membrane neighboring the cluster, the Ca(2+) spark has been shown to be t...

    journal_title:Biophysical journal

    pub_type: 杂志文章


    authors: Sobie EA,Dilly KW,dos Santos Cruz J,Lederer WJ,Jafri MS

    更新日期:2002-07-01 00:00:00

  • Evidence for chaotic behavior in driven ventricles.

    abstract::Toad ventricles were externally driven by periodic pulses while monophasic action potential (MAP) signals were recorded in seven excised and seven in situ ventricles. As the frequency was slowly increased in steps, the stimulated tissue displayed several dynamic characteristics. Hierarchies of periodic behavior, like ...

    journal_title:Biophysical journal

    pub_type: 杂志文章


    authors: Savino GV,Romanelli L,González DL,Piro O,Valentinuzzi ME

    更新日期:1989-08-01 00:00:00

  • Stability and robustness of an organelle number control system: modeling and measuring homeostatic regulation of centriole abundance.

    abstract::Control of organelle abundance is a fundamental unsolved problem in cell biology. Mechanisms for number control have been proposed in which organelle assembly is actively increased or decreased to compensate for deviations from a set-point, but such phenomena have not been experimentally verified. In this report we ex...

    journal_title:Biophysical journal

    pub_type: 杂志文章


    authors: Marshall WF

    更新日期:2007-09-01 00:00:00

  • Mechanisms of propagation of intercellular calcium waves in arterial smooth muscle cells.

    abstract::In rat mesenteric arteries, smooth muscle cells exhibit intercellular calcium waves in response to local phenylephrine stimulation. These waves have a velocity of approximately 20 cells/s and a range of approximately 80 cells. We analyze these waves in a theoretical model of a population of coupled smooth muscle cells...

    journal_title:Biophysical journal

    pub_type: 杂志文章


    authors: Koenigsberger M,Seppey D,Bény JL,Meister JJ

    更新日期:2010-07-21 00:00:00

  • Motor-like Properties of Nonmotor Enzymes.

    abstract::Molecular motors are thought to generate force and directional motion via nonequilibrium switching between energy surfaces. Because all enzymes can undergo such switching, we hypothesized that the ability to generate rotary motion and torque is not unique to highly adapted biological motor proteins but is instead a co...

    journal_title:Biophysical journal

    pub_type: 杂志文章


    authors: Slochower DR,Gilson MK

    更新日期:2018-05-08 00:00:00

  • The thermal inactivation of T4 and lambda bacteriophage.

    abstract::Thermal inactivation of T(4) and lambda bacteriophage shows two components of differing sensitivity are present. These cannot be interpreted as owing to nucleic acid and protein. One protein function-the inhibition of radiation-induced DNA degradation-is lost with quite different thermal kinetics. lambda heated in the...

    journal_title:Biophysical journal

    pub_type: 杂志文章


    authors: Pollard EC,Solosko W

    更新日期:1971-01-01 00:00:00

  • Differential polarization imaging. V. Numerical aperture effects and the contribution of preferential scattering and absorption to the circular dichroism images.

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    journal_title:Biophysical journal

    pub_type: 杂志文章


    authors: Finzi L,Ulibarri L,Bustamante C

    更新日期:1991-06-01 00:00:00

  • Mechanosensitivity Occurs along the Adhesome's Force Train and Affects Traction Stress.

    abstract::Herein, we consider the process of force development along the adhesome within cell focal adhesions. Our model adhesome consists of the actin cytoskeleton-vinculin-talin-integrin-ligand-extracellular matrix-substrate force train. We specifically consider the effects of substrate stiffness on the force levels expected ...

    journal_title:Biophysical journal

    pub_type: 杂志文章


    authors: Asaro RJ,Lin K,Zhu Q

    更新日期:2019-11-05 00:00:00

  • Studies of impedance in cardiac tissue using sucrose gap and computer techniques. II. Circuit simulation of passive electrical properties and cell-to-cell transmission.

    abstract::The impedance measured in a strip of heart tissue from the moth Hyalophora cecropia is fitted by circuit models of several configurations. The circuits include: (a) a single R-C circuit (b) a double R-C circuit (c) terminated transmission lines, and (d) a pattern of cells with cell-to-cell transmission paths. The last...

    journal_title:Biophysical journal

    pub_type: 杂志文章


    authors: Stibitz GR,McCann FV

    更新日期:1974-02-01 00:00:00

  • pH dependence thermal stability of a chymotrypsin inhibitor from Schizolobium parahyba seeds.

    abstract::The thermal stability of a Schizolobium parahyba chymotrypsin inhibitor (SPCI) as a function of pH has been investigated using fluorescence, circular dichroism, and differential scanning calorimetry (DSC). The thermodynamic parameters derived from all methods are remarkably similar and strongly suggest the high stabil...

    journal_title:Biophysical journal

    pub_type: 杂志文章


    authors: Teles RC,Calderon Lde A,Medrano FJ,Barbosa JA,Guimarães BG,Santoro MM,de Freitas SM

    更新日期:2005-05-01 00:00:00

  • Molecular dynamics simulation of carboxy-myoglobin embedded in a trehalose-water matrix.

    abstract::We report on a molecular dynamics (MD) simulation of carboxy-myoglobin (MbCO) embedded in a water-trehalose system. The mean square fluctuations of protein atoms, calculated at different temperatures in the 100-300 K range, are compared with those from a previous MD simulation on an H2O-solvated MbCO and with experime...

    journal_title:Biophysical journal

    pub_type: 杂志文章


    authors: Cottone G,Cordone L,Ciccotti G

    更新日期:2001-02-01 00:00:00

  • Function-related regulation of the stability of MHC proteins.

    abstract::Proteins must be stable to accomplish their biological function and to avoid enzymatic degradation. Constitutive proteolysis, however, is the main source of free amino acids used for de novo protein synthesis. In this paper the delicate balance of protein stability and degradability is discussed in the context of func...

    journal_title:Biophysical journal

    pub_type: 杂志文章


    authors: Simon A,Dosztányi Zs,Rajnavölgyi E,Simon I

    更新日期:2000-11-01 00:00:00

  • Kinetic studies of calcium-induced calcium release in cardiac sarcoplasmic reticulum vesicles.

    abstract::Fast Ca(2+) release kinetics were measured in cardiac sarcoplasmic reticulum vesicles actively loaded with Ca(2+). Release was induced in solutions containing 1.2 mM free ATP and variable free [Ca(2+)] and [Mg(2+)]. Release rate constants (k) were 10-fold higher at pCa 6 than at pCa 5 whereas Ryanodine binding was hig...

    journal_title:Biophysical journal

    pub_type: 杂志文章


    authors: Sánchez G,Hidalgo C,Donoso P

    更新日期:2003-04-01 00:00:00