Abstract:
:We use fluorescence correlation spectroscopy and fluorescence recovery after photobleaching to study vesicle dynamics inside the synapses of cultured hippocampal neurons labeled with the fluorescent vesicle marker FM 1-43. These studies show that when the cell is electrically at rest, only a small population of vesicles is mobile, taking seconds to traverse the synapse. Applying the phosphatase inhibitor okadaic acid causes vesicles to diffuse freely, moving 30 times faster than vesicles in control synapses. These results suggest that vesicles move sluggishly due to binding to elements of the synaptic cytomatrix and that this binding is altered by phosphorylation. Motivated by these results, a model is constructed consisting of diffusing vesicles that bind reversibly to the cytomatrix. This stick-and-diffuse model accounts for the fluorescence correlation spectroscopy and fluorescence recovery after photobleaching data, and also predicts the well-known exponential refilling of the readily releasable pool. Our measurements suggest that the movement of vesicles to the active zone is the rate-limiting step in this process.
journal_name
Biophys Jjournal_title
Biophysical journalauthors
Shtrahman M,Yeung C,Nauen DW,Bi GQ,Wu XLdoi
10.1529/biophysj.105.059295subject
Has Abstractpub_date
2005-11-01 00:00:00pages
3615-27issue
5eissn
0006-3495issn
1542-0086pii
S0006-3495(05)73000-9journal_volume
89pub_type
杂志文章abstract::The interaction of the nonionic surfactant dodecylmaltoside (DM) with phosphatidylcholine liposomes was investigated. Permeability alterations were detected as a change in 5(6)-carboxyfluorescein released from the interior of vesicles and bilayer solubilization as a decrease in the static light scattered by liposome s...
journal_title:Biophysical journal
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journal_title:Biophysical journal
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pub_type: 杂志文章
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journal_title:Biophysical journal
pub_type: 杂志文章
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pub_type: 杂志文章
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更新日期:2020-11-03 00:00:00
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journal_title:Biophysical journal
pub_type: 杂志文章
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更新日期:2008-12-01 00:00:00