Study of anisotropy in nuclear magnetic resonance relaxation times of water protons in skeletal muscle.

abstract：:Molecular recognition between biotinylated bovine serum albumin and polyclonal, biotin-directed IG antibodies has been measured directly under various buffer conditions using an atomic force microscope (AFM). It was found that even highly structured molecules such as IgG antibodies preserve their specific affinity to ...

journal_title：Biophysical journal

authors： Dammer U,Hegner M,Anselmetti D,Wagner P,Dreier M,Huber W,Güntherodt HJ

更新日期：1996-05-01 00:00:00

abstract：:Although actin and myosin are important contributors to cell-force generation, shape change, and motility, their contributions to cell stiffness and frequency-dependent rheology have not been conclusively determined. We apply several pharmacological interventions to cultured epithelial cells to elucidate the roles of ...

journal_title：Biophysical journal

authors： Van Citters KM,Hoffman BD,Massiera G,Crocker JC

更新日期：2006-11-15 00:00:00

abstract：:The kinetics of oxygen geminate binding with the taxonomic substates of MbO2 are reported. The maximum entropy method was used to analyze the rebinding kinetics of MbCO and MbO2 monitored in the Soret. The resulting rate distributions were found to consist of a small number of overlapping bands. A global parametric fi...

journal_title：Biophysical journal

authors： Tetreau C,Novikov E,Tourbez M,Lavalette D

更新日期：2002-04-01 00:00:00

abstract：:Transmissible spongiform encephalopathies are neurodegenerative diseases characterized by the accumulation of an abnormal isoform of the prion protein PrP(Sc). Its fragment 106-126 has been reported to maintain most of the pathological features of PrP(Sc), and a role in neurodegeneration has been proposed based on the...

journal_title：Biophysical journal

authors： Henriques ST,Pattenden LK,Aguilar MI,Castanho MA

更新日期：2008-08-01 00:00:00

abstract：:Globotriaosylceramide (Gb3), a glycosphingolipid found in the plasma membrane of animal cells, is the endocytic receptor of the bacterial Shiga toxin. Using x-ray reflectivity (XR) and grazing incidence x-ray diffraction (GIXD), lipid monolayers containing Gb3 were investigated at the air-water interface. XR probed Gb...

journal_title：Biophysical journal

authors： Watkins EB,Gao H,Dennison AJC,Chopin N,Struth B,Arnold T,Florent JC,Johannes L

更新日期：2014-09-02 00:00:00

abstract：:Intercellular channels formed of members of the gene family of connexins (Cxs) vary from being substantially cation selective to being anion selective. We took advantage of the ability of Cx46 to function as an unopposed hemichannel to examine the basis of Cx charge selectivity. Previously we showed Cx46 hemichannels ...

journal_title：Biophysical journal

authors： Trexler EB,Bukauskas FF,Kronengold J,Bargiello TA,Verselis VK

更新日期：2000-12-01 00:00:00

abstract：:Investigations into the spatiotemporal dynamics of DNA repair using live-cell imaging are aided by the ability to generate well defined regions of ultravioletlike photolesions in an optical microscope. We demonstrate that multiphoton excitation of DNA in live cells with visible femtosecond pulses produces thymine cycl...

journal_title：Biophysical journal

authors： Daddysman MK,Fecko CJ

更新日期：2011-11-02 00:00:00

abstract：:The kinetics of conformational changes of P-type ATPases necessary for the occlusion or deocclusion of transported ions are known to be sensitive to the composition of the surrounding membrane, e.g., phospholipid content, mole percentage of cholesterol, and the presence of lipid-bound anions. Research has shown that m...

journal_title：Biophysical journal

authors： Clarke RJ

更新日期：2015-10-20 00:00:00

abstract：:The effect of a transmembrane peptide on the domain structure of a two-component, two-phase lipid bilayer composed of dimyristoyl phosphatidylcholine (DMPC) and distearoyl phosphatidylcholine (DSPC) was examined by spin label electron spin resonance (ESR) spectroscopy. The peptide, pOmpA, is the hydrophobic, 25-residu...

journal_title：Biophysical journal

authors： Sankaram MB,Marsh D,Gierasch LM,Thompson TE

更新日期：1994-06-01 00:00:00

abstract：:Alkaline sucrose sedimentation studies of DNA from mouse lymphoma cells (L5178Y) treated with caffeine have demonstrated the following effects. Caffeine (at a concentration of 1.6 mM) does not introduce strand breaks into preformed DNA nor does it inhibit the rejoining of gamma-ray-induced strand breaks. Although it d...

journal_title：Biophysical journal

authors： Lehmann AR

更新日期：1972-10-01 00:00:00

abstract：:The capability to image single microbial cell surfaces at nanometer scale under native conditions would profoundly impact mechanistic and structural studies of pathogenesis, immunobiology, environmental resistance, and biotransformation. Here, using in vitro atomic force microscopy, we have directly visualized high-re...

journal_title：Biophysical journal

authors： Plomp M,Leighton TJ,Wheeler KE,Malkin AJ

更新日期：2005-01-01 00:00:00

abstract：:Interactions of membrane anchored molecules such as glycolipids with a membrane surface are important in determining headgroup conformation. It is therefore essential to represent these membrane surface interactions in molecular modeling studies of glycolipids and other membrane bound molecules. We introduce here an e...

journal_title：Biophysical journal

authors： Ram P,Kim E,Thomson DS,Howard KP,Prestegard JH

更新日期：1992-12-01 00:00:00

abstract：:Glucose-embedded bacteriorhodopsin shows M-intermediates with different Amide I infrared bands when samples are illuminated at 240 or 260 K, in contrast with fully hydrated samples where a single M-intermediate is formed at all temperatures. In hydrated, but not in glucose-embedded specimens, the N intermediate is for...

journal_title：Biophysical journal

authors： Vonck J,Han BG,Burkard F,Perkins GA,Glaeser RM

更新日期：1994-09-01 00:00:00

abstract：:Gastrointestinal slow waves are generated within networks of interstitial cells of Cajal (ICCs). In the intact tissue, slow waves are entrained to neighboring ICCs with higher intrinsic frequencies, leading to active propagation of slow waves. Degradation of ICC networks in humans is associated with motility disorders...

journal_title：Biophysical journal

authors： Du P,O'Grady G,Gibbons SJ,Yassi R,Lees-Green R,Farrugia G,Cheng LK,Pullan AJ

更新日期：2010-05-19 00:00:00

abstract：:A mode of internal motion of single tryptophan, Trp 86, of Streptomyces subtilisin inhibitor, was analyzed from its time-resolved fluorescence. The intensity and anisotropy decays of Trp 86 were measured in the picosecond range. These decays were analyzed with theoretical expressions derived assuming that the indole r...

journal_title：Biophysical journal

authors： Tanaka F,Tamai N,Mataga N,Tonomura B,Hiromi K

更新日期：1994-08-01 00:00:00

abstract：:A recently developed technique for in vivo determination of the electrical resistance of vascular endothelium in microvessels was applied to the vessels in a thin frog muscle, m. cutaneus pectoris. The technique consists of injection of current via a glass micropipette into a capillary and measurement of the resulting...

journal_title：Biophysical journal

authors： Olesen SP,Crone C

更新日期：1983-04-01 00:00:00

abstract：:Monolayers of a functional pulmonary surfactant (PS) can reach very low surface tensions well below their equilibrium value. The mechanism by which PS monolayers reach such low surface tensions and maintain film stability remains unknown. As shown previously by fluorescence microscopy, phospholipid phase transition an...

journal_title：Biophysical journal

authors： Zuo YY,Keating E,Zhao L,Tadayyon SM,Veldhuizen RA,Petersen NO,Possmayer F

更新日期：2008-05-01 00:00:00

abstract：:The solubility of aqueous solutions of lysozyme in the presence of polyethylene glycol and various alkaline salts was studied experimentally. The protein-electrolyte mixture was titrated with polyethylene glycol, and when precipitation of the protein occurred, a strong increase of the absorbance at 340 nm was observed...

journal_title：Biophysical journal

authors： Boncina M,Rescic J,Vlachy V

更新日期：2008-08-01 00:00:00

abstract：:Current methods for fluorescence resonance energy transfer (FRET) microscopy of living cells involve taking a series of images with alternating excitation colors in separate camera exposures. Here we present a new FRET method based on polarization that requires only one camera exposure and thereby offers the possibili...

journal_title：Biophysical journal

authors： Mattheyses AL,Hoppe AD,Axelrod D

更新日期：2004-10-01 00:00:00

abstract：:Sister chromatids are topologically intertwined at the onset of anaphase: their segregation during anaphase is known to require strand-passing activity by type II DNA topoisomerase. We propose that the removal of the intertwinings involves at the same time the traction of the mitotic spindle and the activity of topois...

journal_title：Biophysical journal

authors： Duplantier B,Jannink G,Sikorav JL

更新日期：1995-10-01 00:00:00

abstract：:The properties of phosphatidylcholines (PCs) having a perdeuterated stearic acid, 18:0d35, in the sn-1 position and the fatty acid 18:0, 18:1 omega 9, 18:2 omega 6, 18:3 omega 3, 20:4 omega 6, 20:5 omega 3, or 22:6 omega 3 at the sn-2 position were investigated in a matrix of dioleoylphosphatidylethanolamine (DOPE) by...

journal_title：Biophysical journal

authors： Separovic F,Gawrisch K

更新日期：1996-07-01 00:00:00

abstract：:The diffusion of plastoquinol and its binding to the cytochrome bf complex, which occurs during linear photosynthetic electron transport and is analogous to reaction sequences found in most energy-converting membranes, has been studied in intact thylakoid membranes. The flash-induced electron transfer between the late...

journal_title：Biophysical journal

authors： Mitchell R,Spillmann A,Haehnel W

更新日期：1990-10-01 00:00:00

abstract：:Guanine-rich DNA repeat sequences located at the terminal ends of chromosomal DNA can fold in a sequence-dependent manner into G-quadruplex structures, notably the terminal 150-200 nucleotides at the 3' end, which occur as a single-stranded DNA overhang. The crystal structures of quadruplexes with two and four human t...

journal_title：Biophysical journal

authors： Haider S,Parkinson GN,Neidle S

更新日期：2008-07-01 00:00:00

abstract：:In cardiac tissue, during partial blockade of the membrane sodium channels, or at high frequencies of excitation, inexcitable obstacles with sharp edges may destabilize the propagation of electrical excitation waves, causing the formation of self-sustained vortices and turbulent cardiac electrical activity. The format...

journal_title：Biophysical journal

authors： Cabo C,Pertsov AM,Davidenko JM,Baxter WT,Gray RA,Jalife J

更新日期：1996-03-01 00:00:00

abstract：:The crystal structure and a 96-ps molecular dynamics simulation used to analyze structural and motional contributions to spin-lattice (T1) relaxation times of phenylalanine and tyrosine C gamma carbons of the pancreatic trypsin inhibitor. The H beta and H delta protons geminal to C gamma are calculated to account for ...

journal_title：Biophysical journal

authors： Levy RM,Dobson CM,Karplus M

更新日期：1982-07-01 00:00:00

abstract：:A potassium-selective, chemically excitable channel, whose characteristics cannot be accurately described by constant-field theory, is studied by a new approach based on diffusion theory but with no need for the classical assumptions of constant field, homogeneous membrane, and equal phase-boundary potentials at both ...

journal_title：Biophysical journal

authors： Schwartz TL,Kado RT

更新日期：1977-06-01 00:00:00

abstract：:The role of desolvation in protein binding kinetics is investigated using Brownian dynamics simulations in complexes in which the electrostatic interactions are relatively weak. We find that partial desolvation, modeled by a short-range atomic contact potential, is not only a major contributor to the binding free ener...

journal_title：Biophysical journal

authors： Camacho CJ,Kimura SR,DeLisi C,Vajda S

更新日期：2000-03-01 00:00:00

abstract：:Experimental studies have recently demonstrated that fluorescence emission can be quenched by laser light pulses from modern high repetition rate lasers, a phenomenon we call "light quenching." We now describe the theory of light quenching and some of its effects on the steady-state and time-resolved intensity and ani...

journal_title：Biophysical journal

authors： Kuśba J,Bogdanov V,Gryczynski I,Lakowicz JR

更新日期：1994-11-01 00:00:00

abstract：:Single-molecule epifluorescence microscopy was used to observe the translational motion of GPI-linked and native I-E(k) class II MHC membrane proteins in the plasma membrane of CHO cells. The purpose of the study was to look for deviations from Brownian diffusion that might arise from barriers to this motion. Detergen...

journal_title：Biophysical journal

authors： Vrljic M,Nishimura SY,Brasselet S,Moerner WE,McConnell HM

更新日期：2002-11-01 00:00:00

abstract：:A sequence determinant of reduction potentials is reported for bacterial [4Fe-4S]-type ferredoxins. The residue that is four residues C-terminal to the fourth ligand of either cluster is generally an alanine or a cysteine. In five experimental ferredoxin structures, the cysteine has the same structural orientation rel...

journal_title：Biophysical journal

authors： Beck BW,Xie Q,Ichiye T

更新日期：2001-08-01 00:00:00