Abstract:
:More and more researchers are interested in imaging living (Henderson, 1994) or fixed cells in their natural environment using the atomic force microscope (AFM). However, the AFM tip interacts strongly with the sample, and its z range freedom is limited to a few micrometers. This means that the cells to be imaged have to be strongly attached to the substrate, and imaging is restricted to cells having a flattened shape. Here we propose a simple and inexpensive solution to overcome these limitations. The method we propose is trapping living round shaped cells in a Millipore filter with a pore size comparable to the dimensions of the cell. The highest part of some of the blocked cells protrude through the holes of the filter and can this way be easily observed using the AFM without detachment.
journal_name
Biophys Jjournal_title
Biophysical journalauthors
Kasas S,Ikai Adoi
10.1016/S0006-3495(95)80344-9subject
Has Abstractpub_date
1995-05-01 00:00:00pages
1678-80issue
5eissn
0006-3495issn
1542-0086pii
S0006-3495(95)80344-9journal_volume
68pub_type
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