Molecular heterogeneity of O-acetylserine sulfhydrylase by two-photon excited fluorescence fluctuation spectroscopy.

abstract：:Detection algorithms are widely used for the analysis of single-channel data because they remove the background noise from the measured current signal and reconstruct the noise-free time series. Standard detection algorithms assume channels switching only between zero and full conductance. Many types of channels, howe...

journal_title：Biophysical journal

authors： Draber S,Schultze R

更新日期：1994-10-01 00:00:00

abstract：:The physical forces that underlie the exclusion of solutes from macromolecular surfaces can be probed in a similar way as the measurement of forces between macromolecules in condensed arrays using the osmotic stress technique and x-ray scattering. We report here the dependence of alcohol exclusion or, equivalently, th...

journal_title：Biophysical journal

authors： Stanley C,Rau DC

更新日期：2006-08-01 00:00:00

abstract：:A block normal mode (BNM) algorithm, originally proposed by Tama et al., (Proteins Struct. Func. Genet. 41:1-7, 2000) was implemented into the simulation program CHARMM. The BNM approach projects the hessian matrix into local translation/rotation basis vectors and, therefore, dramatically reduces the size of the matri...

journal_title：Biophysical journal

authors： Li G,Cui Q

更新日期：2002-11-01 00:00:00

abstract：:Homooligomerization of proline utilization A (PutA) bifunctional flavoenzymes is intimately tied to catalytic function and substrate channeling. PutA from Bradyrhizobium japonicum (BjPutA) is unique among PutAs in that it forms a tetramer in solution. Curiously, a dimeric BjPutA hot spot mutant was previously shown to...

journal_title：Biophysical journal

authors： Korasick DA,Campbell AC,Christgen SL,Chakravarthy S,White TA,Becker DF,Tanner JJ

更新日期：2018-06-19 00:00:00

abstract：:Aquaporin (AQP) functions as a water-conducting pore. Mercury inhibits the water permeation through AQP. Although site-directed mutagenesis has shown that mercury binds to Cys189 during the inhibition process, it is not fully understood how this inhibits the water permeation through AQP1. We carried out 40 ns molecula...

journal_title：Biophysical journal

authors： Hirano Y,Okimoto N,Kadohira I,Suematsu M,Yasuoka K,Yasui M

更新日期：2010-04-21 00:00:00

abstract：:Extracellular vesicles (EVs) are proposed to play important roles in intercellular communication. Two classes of EVs can be distinguished based on their intracellular origin. Exosomes are generated within endosomes and released when these fuse with the plasma membrane, whereas ectosomes bud directly from the plasma me...

journal_title：Biophysical journal

authors： Jackson CE,Scruggs BS,Schaffer JE,Hanson PI

更新日期：2017-09-19 00:00:00

abstract：:Although cell-cell interactions are known to significantly affect the kinetics of intracellular ice formation (IIF) during tissue freezing, this effect is not well understood. Progress in elucidating the mechanism and role of intercellular ice propagation in tissue freezing has been hampered in part by limitations in ...

journal_title：Biophysical journal

authors： Irimia D,Karlsson JO

更新日期：2005-01-01 00:00:00

abstract：:An approach is described using fluorescence resonance energy transfer (FRET) to detect inhomogeneity in lipid organization, on distance scales of the order of tens of nanometers or greater, in lipid bilayers. This approach compares the efficiency of energy transfer between two matched fluorescent lipid donors, differi...

journal_title：Biophysical journal

authors： Silvius JR

更新日期：2003-08-01 00:00:00

abstract：:The cytoskeletal protein MreB is an essential component of the bacterial cell-shape generation system. Using a superresolution variant of total internal reflection microscopy with structured illumination, as well as three-dimensional stacks of deconvolved epifluorescence microscopy, we found that inside living Bacillu...

journal_title：Biophysical journal

authors： Olshausen PV,Defeu Soufo HJ,Wicker K,Heintzmann R,Graumann PL,Rohrbach A

更新日期：2013-09-03 00:00:00

abstract：:Intrinsically disordered proteins play important roles throughout biology, yet our understanding of the relationship between their sequences, structural properties, and functions remains incomplete. The dynamic nature of these proteins, however, makes them difficult to characterize structurally. Many disordered protei...

journal_title：Biophysical journal

authors： Nygaard M,Kragelund BB,Papaleo E,Lindorff-Larsen K

更新日期：2017-08-08 00:00:00

abstract：:We investigate a temperature-driven recursive division of binary giant unilamellar vesicles (GUVs). During the heating step of the heating-cooling cycle, the spherical mother vesicle deforms to a budded limiting shape using up the excess area produced by the chain melting of the lipids and then splits off into two dau...

journal_title：Biophysical journal

authors： Jimbo T,Sakuma Y,Urakami N,Ziherl P,Imai M

更新日期：2016-04-12 00:00:00

abstract：:The photoelectron quantum yields of 21 common amino acids and 15 polyamino acids were measured in the 180-240 nm wavelength region. On the average, the quantum yields of these two groups exhibit quite similar wavelength dependence. For lambda > 220 nm all amino acid and polyamino acid quantum yields are

journal_title：Biophysical journal

authors： Dam RJ,Burke CA,Griffith OH

更新日期：1974-06-01 00:00:00

abstract：:Rapid conduction of nerve impulses requires coating of axons by myelin sheaths, which are multilamellar, lipid-rich membranes produced by oligodendrocytes in the central nervous system. To act as an insulator, myelin has to form a stable and firm membrane structure. In this study, we have analyzed the biophysical prop...

journal_title：Biophysical journal

authors： Yurlova L,Kahya N,Aggarwal S,Kaiser HJ,Chiantia S,Bakhti M,Pewzner-Jung Y,Ben-David O,Futerman AH,Brügger B,Simons M

更新日期：2011-12-07 00:00:00

abstract：:The oligomeric structure was determined for four recombinant kinesin derivatives containing N-terminal fragments of the kinesin alpha-subunit. Some of the proteins were dimeric (two-headed) molecules with mechanochemical properties similar to those of intact kinesin. Comparison of the primary and quaternary structures...

journal_title：Biophysical journal

authors： Gelles J,Berliner E,Young EC,Mahtani HK,Perez-Ramirez B,Anderson K

更新日期：1995-04-01 00:00:00

abstract：:The relationship between calcium current and transmitter release was studied in squid giant synapse. It was found that the voltage-dependent calcium current triggers the release of synaptic transmitter in direct proportion to its magnitude and duration. Transmitter release occurs with a delay of approximately 200 mus ...

journal_title：Biophysical journal

authors： Llinás R,Steinberg IZ,Walton K

更新日期：1981-03-01 00:00:00

abstract：:When a dissolved macromolecule is in chemical equilibrium with a free ionic species, the charge configuration, and hence the dipole vector, of the macromolecule is fluctuating. Expressions for the static dielectric constant and the relaxation spectrum of such a mixture are here derived in terms of the components of th...

journal_title：Biophysical journal

authors： Scheider W

更新日期：1965-09-01 00:00:00

abstract：:Conserved active-site elements in myosins and other P-loop NTPases play critical roles in nucleotide binding and hydrolysis; however, the mechanisms of allosteric communication among these mechanoenzymes remain unresolved. In this work we introduced the E442A mutation, which abrogates a salt-bridge between switch I an...

journal_title：Biophysical journal

authors： Trivedi DV,David C,Jacobs DJ,Yengo CM

更新日期：2012-06-06 00:00:00

abstract：:How do Ca channels conduct Ca ions during the cardiac action potential? We attempt to answer this question by applying a two-microelectrode technique, previously used for Na and K currents, in which we record the patch current and the action potential at the same time (Mazzanti, M., and L. J. DeFelice. 1987. Biophys. ...

journal_title：Biophysical journal

authors： Mazzanti M,DeFelice LJ

更新日期：1990-10-01 00:00:00

abstract：:Human posttranslationally modified N-ras oncogenes are known to be implicated in numerous human cancers. Here, we applied a combination of experimental and computational techniques to determine structural and dynamical details of the lipid chain modifications of an N-ras heptapeptide in 1,2-dimyristoyl-sn-glycero-3-ph...

journal_title：Biophysical journal

authors： Vogel A,Tan KT,Waldmann H,Feller SE,Brown MF,Huster D

更新日期：2007-10-15 00:00:00

abstract：:The mechanism for transport of divalent cations across phospholipid bilayers by the ionophore A23187 was investigated. The intrinsic fluorescence of the ionophore was used in equilibrium and rapid-mixing experiments as an indicator of ionophore environment and complexation with divalent cations. The neutral (protonate...

journal_title：Biophysical journal

authors： Kolber MA,Haynes DH

更新日期：1981-11-01 00:00:00

abstract：:Hormones are released from neuroendocrine cells by passing through an exocytotic pore that forms after vesicle and plasma membrane fusion. An elegant way to study this process at the single-vesicle level is to use styryl dyes, which stain not only the membrane, but also the matrix of individual vesicles in some neuroe...

journal_title：Biophysical journal

authors： Stenovec M,Poberaj I,Kreft M,Zorec R

更新日期：2005-04-01 00:00:00

abstract：:Steady state fluorescence polarization spectra and time-resolved emission decay kinetics have been measured in vitro from malignant and normal rat kidney tissue. The degrees of polarization and emission lifetimes from the cancerous and normal systems are different. The spectroscopic differences are attributed to envir...

journal_title：Biophysical journal

authors： Tata DB,Foresti M,Cordero J,Tomashefsky P,Alfano MA,Alfano RR

更新日期：1986-09-01 00:00:00

abstract：:An analysis is presented of the complex anisotropy behavior of trans-parinaric acid in single component DEPC lipid bilayers. It is shown that a model involving two species with distinct lifetime and motional behavior is required, and is adequate, to explain the observed data. In particular, the observed increase in th...

journal_title：Biophysical journal

authors： Ruggiero A,Hudson B

更新日期：1989-06-01 00:00:00

abstract：:Certain environmental parameters are accessible to cells only indirectly and require an encoding step for cells to retrieve the relevant information. A prominent example is the phenomenon of quorum sensing by microorganisms, where information about cell density is encoded by means of secreted signaling molecules. The ...

journal_title：Biophysical journal

authors： Drees B,Reiger M,Jung K,Bischofs IB

更新日期：2014-07-01 00:00:00

abstract：:Metallic nanoparticles have opened the possibility of imaging, tracking, and manipulating biological samples without time limitations. Their low photoluminescence quantum yield, however, makes them hard to detect under high background conditions. In this study we show that it is possible to image gold nanorods by dete...

journal_title：Biophysical journal

authors： Carattino A,Keizer VIP,Schaaf MJM,Orrit M

更新日期：2016-12-06 00:00:00

abstract：:It has long been appreciated that spatiotemporal dynamics of cell migration are under the control of intracellular signaling pathways, which are mediated by adhesion receptors and other transducers of extracellular cues. Further, there is ample evidence that aspects of cell migration are stochastic: how else could it ...

journal_title：Biophysical journal

authors： Weiger MC,Ahmed S,Welf ES,Haugh JM

更新日期：2010-01-06 00:00:00

abstract：:Myxicola giant axons internally injected with tetraethylammonium chloride to block potassium currents were examined under voltage clamp. The sodium inactivation time constants obtained from the decline in INa during step depolarizations were substantially smaller than those obtained using conditioning prepulses to the...

journal_title：Biophysical journal

authors： Schauf CL,Pencek TL,Davis FA

更新日期：1976-09-01 00:00:00

abstract：:Equatorial x-ray diffraction patterns were obtained from single skinned rabbit psoas fibers during various degrees of activation under isometric conditions at ionic strength 170 mM and 6-9 degrees C. By direct calcium activation, contraction was homogeneous throughout the preparation, and by using a cycling technique ...

journal_title：Biophysical journal

authors： Brenner B,Yu LC

更新日期：1985-11-01 00:00:00

abstract：:DNA release from lipoplexes is an essential step during lipofection and is probably a result of charge neutralization by cellular anionic lipids. As a model system to test this possibility, fluorescence resonance energy transfer between DNA and lipid covalently labeled with Cy3 and BODIPY, respectively, was used to mo...

journal_title：Biophysical journal

authors： Tarahovsky YS,Koynova R,MacDonald RC

更新日期：2004-08-01 00:00:00

abstract：:The pore-forming alpha 1 subunit of L-type calcium (Ca2+) channels is the molecular target of Ca2+ channel blockers such as phenylalkylamines (PAAs). Association and dissociation rates of (-)devapamil were compared for a highly PAA-sensitive L-type Ca2+ channel chimera (Lh) and various class A Ca2+ channel mutants. Th...

journal_title：Biophysical journal

authors： Degtiar VE,Aczél S,Döring F,Timin EN,Berjukow S,Kimball D,Mitterdorfer J,Hering S

更新日期：1997-07-01 00:00:00