Analysis of the anisotropy decay of trans-parinaric acid in lipid bilayers.


:An analysis is presented of the complex anisotropy behavior of trans-parinaric acid in single component DEPC lipid bilayers. It is shown that a model involving two species with distinct lifetime and motional behavior is required, and is adequate, to explain the observed data. In particular, the observed increase in the anisotropy at long times demonstrates the presence of a species with a long fluorescence lifetime that has a high anisotropy. The time dependence of the anisotropy for these two environments is treated using both a purely mathematical sum of exponentials and a constrained fit based on an approximate solution of the anisotropic diffusion problem. In this latter model the anisotropy is described in terms of the second and fourth rank order parameters, (P2) and (P4), and a single dynamical parameter, D1, the perpendicular diffusion coefficient for this uniaxial probe. The parameters of both models are accurately determined from the fits to the data when two environments coexist and an association is made between lifetime components and distinct rotational sites. The values of the parameters obtained demonstrate the "solid-like" and "fluidlike" nature of these two coexisting environments.


Biophys J


Biophysical journal


Ruggiero A,Hudson B




Has Abstract


1989-06-01 00:00:00














  • A computational framework for the topological analysis and targeted disruption of signal transduction networks.

    abstract::In this article, optimization-based frameworks are introduced for elucidating the input-output structure of signaling networks and for pinpointing targeted disruptions leading to the silencing of undesirable outputs in therapeutic interventions. The frameworks are demonstrated on a large-scale reconstruction of a sign...

    journal_title:Biophysical journal

    pub_type: 杂志文章


    authors: Dasika MS,Burgard A,Maranas CD

    更新日期:2006-07-01 00:00:00

  • Measuring the Refractive Index of Bovine Corneal Stromal Cells Using Quantitative Phase Imaging.

    abstract::The cornea is the primary refractive lens in the eye and transmits >90% of incident visible light. It has been suggested that the development of postoperative corneal haze could be due to an increase in light scattering from activated corneal stromal cells. Quiescent keratocytes are thought to produce crystallins that...

    journal_title:Biophysical journal

    pub_type: 杂志文章


    authors: Gardner SJ,White N,Albon J,Knupp C,Kamma-Lorger CS,Meek KM

    更新日期:2015-10-20 00:00:00

  • Comparative Study of the Condensing Effects of Ergosterol and Cholesterol.

    abstract::Cholesterol, due to its condensing effect, is considered an important regulator of membrane thickness. Other sterols, due to their structural similarities to cholesterol, are often assumed to have a universal effect on membrane properties similar to the condensing effect of cholesterol, albeit possibly to different de...

    journal_title:Biophysical journal

    pub_type: 杂志文章


    authors: Hung WC,Lee MT,Chung H,Sun YT,Chen H,Charron NE,Huang HW

    更新日期:2016-05-10 00:00:00

  • Determination of pore-lining residues in the hepatitis C virus p7 protein.

    abstract::The p7 protein from hepatitis C virus is critical for the assembly and secretion of infectious virus, making it an attractive drug target. It is thought to be a viroporin with a demonstrated ion channel activity when reconstituted into planar lipid bilayers. Electron microscopy experiments suggest that p7 oligomers co...

    journal_title:Biophysical journal

    pub_type: 信件


    authors: Chew CF,Vijayan R,Chang J,Zitzmann N,Biggin PC

    更新日期:2009-01-01 00:00:00

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    abstract::The interactions between phospholipid molecules in suspensions have been studied by using mass spectrometry. Electrospray mass spectra of homogeneous preparations formed from three different phospholipid molecules demonstrate that under certain conditions interactions between 90 and 100 lipid molecules can be preserve...

    journal_title:Biophysical journal

    pub_type: 杂志文章


    authors: Hanson CL,Ilag LL,Malo J,Hatters DM,Howlett GJ,Robinson CV

    更新日期:2003-12-01 00:00:00

  • Residues in Na(+) channel D3-S6 segment modulate both batrachotoxin and local anesthetic affinities.

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    journal_title:Biophysical journal

    pub_type: 杂志文章


    authors: Wang SY,Nau C,Wang GK

    更新日期:2000-09-01 00:00:00

  • Limitations of the whole cell patch clamp technique in the control of intracellular concentrations.

    abstract::Recent experimental studies (Pusch and Neher, 1988) and theoretical studies (Oliva et al., 1988) have found that the pipette tip is a significant barrier to diffusion in the whole cell patch clamp configuration. In this paper, we extend the theoretical analysis of fluxes between the pipette and cell to include transme...

    journal_title:Biophysical journal

    pub_type: 杂志文章


    authors: Mathias RT,Cohen IS,Oliva C

    更新日期:1990-09-01 00:00:00

  • Ab initio study of the role of lysine 16 for the molecular switching mechanism of Ras protein p21.

    abstract::Quantum chemical computations using the ab initio molecular orbital (MO) method have been performed to investigate the molecular switching mechanism of Ras protein p21, which has an important role in intracellular signal cascades. Lys(16) was demonstrated to be crucial to the function of Ras p21, and the hydrolysis of...

    journal_title:Biophysical journal

    pub_type: 杂志文章


    authors: Futatsugi N,Hata M,Hoshino T,Tsuda M

    更新日期:1999-12-01 00:00:00

  • Elasticity of the red cell membrane and its relation to hemolytic disorders: an optical tweezers study.

    abstract::We have used optical tweezers to study the elasticity of red cell membranes; force was applied to a bead attached to a permeabilized spherical ghost and the force-extension relation was obtained from the response of a second bead bound at a diametrically opposite position. Interruption of the skeletal network by disso...

    journal_title:Biophysical journal

    pub_type: 杂志文章


    authors: Sleep J,Wilson D,Simmons R,Gratzer W

    更新日期:1999-12-01 00:00:00

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    journal_title:Biophysical journal

    pub_type: 杂志文章


    authors: Inoue K,Tsuda M,Terazima M

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  • Electron-spin resonance study of aggregation of gramicidin in dipalmitoylphosphatidylcholine bilayers and hydrophobic mismatch.

    abstract::The effect of aggregation of gramicidin A' (GA) on the phase structure of dipalmitoylphosphatidylcholine (DPPC) multilamellar vesicles was studied by cw-ESR using a chain-labeled lipid (16PC) at temperatures between 30 degrees and 45 degreesC that span the main phase transition of DPPC. Boundary lipids were observed o...

    journal_title:Biophysical journal

    pub_type: 杂志文章


    authors: Ge M,Freed JH

    更新日期:1999-01-01 00:00:00

  • Nanoparticles as fluorescence labels: is size all that matters?

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    journal_title:Biophysical journal

    pub_type: 杂志文章


    authors: Swift JL,Cramb DT

    更新日期:2008-07-01 00:00:00

  • A quantitative description of equilibrium and homeostatic thickness regulation in the in vivo cornea. I. Normal cornea.

    abstract::By combining a description of the coupled solute and water flows through the in vivo cornea with a set of appropriate mechanical equilibrium conditions, it is possible to calculate directly the corneal thickness, given the corneal temperature, the state of the aqueous and tears, the swelling pressure-hydration relatio...

    journal_title:Biophysical journal

    pub_type: 杂志文章


    authors: Friedman MH

    更新日期:1972-06-01 00:00:00

  • Photoisomerization and photoionization of the photoactive yellow protein chromophore in solution.

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    journal_title:Biophysical journal

    pub_type: 杂志文章


    authors: Larsen DS,Vengris M,van Stokkum IH,van der Horst MA,de Weerd FL,Hellingwerf KJ,van Grondelle R

    更新日期:2004-04-01 00:00:00

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    journal_title:Biophysical journal

    pub_type: 杂志文章


    authors: Badger J,Kapulsky A,Gursky O,Bhyravbhatla B,Caspar DL

    更新日期:1994-02-01 00:00:00

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    journal_title:Biophysical journal

    pub_type: 杂志文章


    authors: Sanders CR 2nd,Schwonek JP

    更新日期:1993-10-01 00:00:00

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    journal_title:Biophysical journal

    pub_type: 杂志文章


    authors: Brock R,Hink MA,Jovin TM

    更新日期:1998-11-01 00:00:00

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    journal_title:Biophysical journal

    pub_type: 杂志文章


    authors: Cantino ME,Quintanilla A

    更新日期:2007-01-15 00:00:00

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    journal_title:Biophysical journal

    pub_type: 杂志文章


    authors: Despa S,Brette F,Orchard CH,Bers DM

    更新日期:2003-11-01 00:00:00

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    abstract::Theoretical models predict that macromolecular crowding can increase protein folding stability, but depending on details of the models (e.g., how the denatured state is represented), the level of stabilization predicted can be very different. In this study, we represented the native and denatured states atomistically,...

    journal_title:Biophysical journal

    pub_type: 杂志文章


    authors: Qin S,Zhou HX

    更新日期:2009-07-08 00:00:00

  • The inhibition of bacteriophage DNA injection by dyes bound to the DNA.

    abstract::A delay ( approximately 10 min) in the appearance of intracellular phage is caused by preincubating the infecting phage T4o(1) in proflavine, acridine orange, or ethidium, but not polyamines. No significant delay in attachment is observed. Apparently the presence of the dye is required inside the permeability barrier ...

    journal_title:Biophysical journal

    pub_type: 杂志文章


    authors: Dusenbery DB,Uretz RB

    更新日期:1972-08-01 00:00:00

  • Prediction of protein relative enthalpic stability from molecular dynamics simulations of the folded and unfolded states.

    abstract::For proteins of known structure, the relative enthalpic stability with respect to wild-type, ΔΔH(U), can be estimated by direct computation of the folded and unfolded state energies. We propose a model by which the change in stability upon mutation can be predicted from all-atom molecular dynamics simulations for the ...

    journal_title:Biophysical journal

    pub_type: 杂志文章


    authors: Dadarlat VM,Gorenstein LA,Post CB

    更新日期:2012-10-17 00:00:00

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    abstract::A detailed treatment is provided of the various free-energy terms that contribute to the transfer of a polyalanine alpha-helix from the aqueous phase into lipid bilayers. In agreement with previous work, the hydrophobic effect is found to provide the major driving force for helix insertion. However, an opposing effect...

    journal_title:Biophysical journal

    pub_type: 杂志文章


    authors: Ben-Tal N,Ben-Shaul A,Nicholls A,Honig B

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    journal_title:Biophysical journal

    pub_type: 杂志文章


    authors: Cope FW

    更新日期:1969-03-01 00:00:00

  • An x-ray diffraction study on early structural changes in skeletal muscle contraction.

    abstract::Structural changes in frog skeletal muscle were studied using x-ray diffraction with a time resolution of 0.53-1.02 ms after a single electrical stimulus at 8 degrees C. Tension began to drop at 6 ms (latency relaxation), reached a minimum at 8 ms, and then twitch tension developed. The intensity of the meridional ref...

    journal_title:Biophysical journal

    pub_type: 杂志文章


    authors: Yagi N

    更新日期:2003-02-01 00:00:00

  • Optical band splitting and electronic perturbations of the heme chromophore in cytochrome C at room temperature probed by visible electronic circular dichroism spectroscopy.

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    journal_title:Biophysical journal

    pub_type: 杂志文章


    authors: Dragomir I,Hagarman A,Wallace C,Schweitzer-Stenner R

    更新日期:2007-02-01 00:00:00

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    journal_title:Biophysical journal

    pub_type: 杂志文章


    authors: Aynechi T,Kuntz ID

    更新日期:2005-11-01 00:00:00

  • Dominant Driving Forces in Human Telomere Quadruplex Binding-Induced Structural Alterations.

    abstract::Recently various pathways of human telomere (ht) DNA folding into G-quadruplexes and of ligand binding to these structures have been proposed. However, the key issue as to the nature of forces driving the folding and recognition processes remains unanswered. In this study, structural changes of 22-mer ht-DNA fragment ...

    journal_title:Biophysical journal

    pub_type: 杂志文章


    authors: Bončina M,Hamon F,Islam B,Teulade-Fichou MP,Vesnaver G,Haider S,Lah J

    更新日期:2015-06-16 00:00:00

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    journal_title:Biophysical journal

    pub_type: 杂志文章


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    更新日期:1973-09-01 00:00:00

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    journal_title:Biophysical journal

    pub_type: 杂志文章


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    更新日期:2007-07-15 00:00:00