Constitutive equations of skeletal muscle based on cross-bridge mechanism.

abstract：:Coat protein (COP) I and COP II complexes are involved in the transport of proteins between the endoplasmic reticulum and the Golgi apparatus in eukaryotic cells. The formation of COP I/II complexes at membrane surfaces is an early step in vesicle formation and is mastered by p24, a type I transmembrane protein. Oligo...

journal_title：Biophysical journal

authors： Pannwitt S,Stangl M,Schneider D

更新日期：2019-11-05 00:00:00

abstract：:The structure and biophysical properties of lipid membranes are important for cellular functions in health and disease. In Alzheimer's disease, the neuronal membrane is a target for toxic amyloid-β (Aβ). Melatonin is an important pineal gland hormone that has been shown to protect against Aβ toxicity in cellular and a...

journal_title：Biophysical journal

authors： Mei N,Robinson M,Davis JH,Leonenko Z

更新日期：2020-12-15 00:00:00

abstract：:The spatiotemporal organization and dynamics of the plasma membrane and its constituents are central to cellular function. Fluorescence-based single-particle tracking has emerged as a powerful approach for studying the single molecule behavior of plasma-membrane-associated events because of its excellent background su...

journal_title：Biophysical journal

authors： de Wit G,Albrecht D,Ewers H,Kukura P

更新日期：2018-06-19 00:00:00

abstract：:Many experiments have measured the effect of force on the dissociation of single selectin bonds, but it is not yet clear how the force dependence of molecular dissociation can influence the rolling of cells expressing selectin molecules. Recent experiments using constant-force atomic force microscopy or high-resolutio...

journal_title：Biophysical journal

authors： Caputo KE,Lee D,King MR,Hammer DA

更新日期：2007-02-01 00:00:00

abstract：:Gaining information about receptor profiles on cells, and subsequently finding the most efficient ligands for these signaling receptors, remain challenging tasks in stem cell and cancer research as well as drug development. We introduce a live-cell method with great potential in both screening for surface receptors an...

journal_title：Biophysical journal

authors： Wienken U,Gaub HE

更新日期：2013-12-17 00:00:00

abstract：:The physical forces that underlie the exclusion of solutes from macromolecular surfaces can be probed in a similar way as the measurement of forces between macromolecules in condensed arrays using the osmotic stress technique and x-ray scattering. We report here the dependence of alcohol exclusion or, equivalently, th...

journal_title：Biophysical journal

authors： Stanley C,Rau DC

更新日期：2006-08-01 00:00:00

abstract：:In this article, we demonstrate the new method of pulsed interleaved excitation (PIE), which can be used to extend the capabilities of multiple-color fluorescence imaging, fluorescence cross-correlation spectroscopy (FCCS), and single-pair fluorescence resonance energy transfer (spFRET) measurements. In PIE, multiple ...

journal_title：Biophysical journal

authors： Müller BK,Zaychikov E,Bräuchle C,Lamb DC

更新日期：2005-11-01 00:00:00

abstract：:Surface and subsurface dynamics of Rat Basophilic Leukemia cells, a model system of stimulated secretion, were imaged using Scanning Force Microscopy (SFM) at a rate of 50-60 s/image. Cytoskeletal elements and organelles were tracked within quiescent cells and those activated after IgE receptor crosslinking. In additi...

journal_title：Biophysical journal

authors： Braunstein D,Spudich A

更新日期：1994-05-01 00:00:00

abstract：:Solid phase amplification (SPA), a new method to amplify DNA, is characterized by the use of surface-bound primers. This limits the amplification to two-dimensional surfaces and therefore allows the easy parallelization of DNA amplification in a single system. SPA leads to the formation of small but dense DNA brushes,...

journal_title：Biophysical journal

authors： Mercier JF,Slater GW

更新日期：2005-07-01 00:00:00

abstract：:Muscle contraction occurs as the result of actin-myosin interaction, which is mediated by the intermolecular forces exerted at the actin-myosin interface. To obtain information about the nature of these intermolecular forces, we tested the sensitivity of various contractile parameters of skinned skeletal muscle fibers...

journal_title：Biophysical journal

authors： Iwamoto H

更新日期：2000-06-01 00:00:00

abstract：:Membranes made of dimyristoylphosphatidylcholine and cholesterol, one of the simplest paradigms for the study of liquid ordered-disordered phase separation, were investigated using a pulse-EPR spin-labeling method in which bimolecular collision of molecular oxygen with the nitroxide spin label is measured. This method...

journal_title：Biophysical journal

authors： Subczynski WK,Wisniewska A,Hyde JS,Kusumi A

更新日期：2007-03-01 00:00:00

abstract：:More than 30 years ago, Nozaki and Tanford reported that the pK values for several amino acids and simple substances in 6 M guanidinium chloride differed little from the corresponding values in low salt (Nozaki, Y., and C. Tanford. 1967. J. Am. Chem. Soc. 89:736-742). This puzzling and counter-intuitive result hinders...

journal_title：Biophysical journal

authors： Garcia-Mira MM,Sanchez-Ruiz JM

更新日期：2001-12-01 00:00:00

abstract：:Disruption of the integrity of the plasma membrane by amyloidogenic proteins is linked to the pathogenesis of a number of common age-related diseases. Although accumulating evidence suggests that adverse environmental stressors such as unbalanced levels of metal ions may trigger amyloid-mediated membrane damage, many ...

journal_title：Biophysical journal

authors： Sciacca MF,Milardi D,Messina GM,Marletta G,Brender JR,Ramamoorthy A,La Rosa C

更新日期：2013-01-08 00:00:00

abstract：:Guanidinium and acetamidinium, when added to the bathing solution in concentrations of approximately 0.1M, cause brief blocks in the single channel potassium currents from channels formed in planar lipid bilayers by gramicidin A. Single channel lifetimes are not affected indicating that the channel structure is not mo...

journal_title：Biophysical journal

authors： Hemsley G,Busath D

更新日期：1991-04-01 00:00:00

abstract：:The mechanism of high-voltage pulse-induced permeabilization of the stratum corneum, the outer layer of the skin, is still not completely understood. It has been suggested that joule heating resulting from the applied pulse may play a major role in disrupting the stratum corneum. In this study, electrical and ultrastr...

journal_title：Biophysical journal

authors： Gallo SA,Sen A,Hensen ML,Hui SW

更新日期：2002-01-01 00:00:00

abstract：:Starzak and Starzak (1978. IEEE Trans. Biomed. Eng. 25:201-204.) proposed that, in cyclic application of current and voltage-clamps, the fidelity of the match of the output current with the original stimulus could be used to measure the spatial uniformity of voltage in a membrane. However, they failed to find such a m...

journal_title：Biophysical journal

authors： Moore JW,Stockbridge N,Schiff SJ

更新日期：1982-12-01 00:00:00

abstract：:Intracellular cargo transport frequently involves multiple motor types, either having opposite directionality or having the same directionality but different speeds. Although significant progress has been made in characterizing kinesin motors at the single-molecule level, predicting their ensemble behavior is challeng...

journal_title：Biophysical journal

authors： Arpağ G,Shastry S,Hancock WO,Tüzel E

更新日期：2014-10-21 00:00:00

abstract：:Forces applied by intact mucus-propelling cilia were measured for the first time that we know of using a combined atomic force microscopy (AFM) and electrooptic system. The AFM probe was dipped into a field of beating cilia and its time-dependent deflection was recorded as it was struck by the cilia while the electroo...

journal_title：Biophysical journal

authors： Teff Z,Priel Z,Gheber LA

更新日期：2007-03-01 00:00:00

abstract：:There is increasing evidence that UVA radiation, which makes up approximately 95% of the solar UV light reaching the Earth's surface and is also commonly used for cosmetic purposes, is genotoxic. However, in contrast to UVC and UVB, the mechanisms by which UVA produces various DNA lesions are still unclear. In additio...

journal_title：Biophysical journal

authors： Jiang Y,Rabbi M,Kim M,Ke C,Lee W,Clark RL,Mieczkowski PA,Marszalek PE

更新日期：2009-02-01 00:00:00

abstract：:The structural features of SPM that control the transbilayer distribution of beta-GalCer in POPC vesicles were investigated by (13)C- and (31)P-NMR spectroscopy using lipid analogs that share physical similarities with GalCer or SPM. The SPM analogs included N-palmitoyl-4,5-dihydro-SPM, 3-deoxy-SPM, 1-alkyl-2-amidopho...

journal_title：Biophysical journal

authors： Malewicz B,Valiyaveettil JT,Jacob K,Byun HS,Mattjus P,Baumann WJ,Bittman R,Brown RE

更新日期：2005-04-01 00:00:00

abstract：:A simple formula for the expansion rate U of the bilayer membrane perimeter was derived by the solution of the hydrodynamic and the Laplace equations at small rates and small contact angles: U = theta delta/6 micron ln (h/e 3/2 h0), where theta is the equilibrium contact angle between the bilayer membrane and the Plat...

journal_title：Biophysical journal

authors： Dimitrov DS

更新日期：1981-10-01 00:00:00

abstract：:A discussion is given of the general role of diffusion in enzyme kinetics based upon a rigorous theory for bimolecular association and dissociation steps that was presented previously, and criteria are formulated for the dependence of the over-all rate on medium viscosity. With these criteria it is possible to conclud...

journal_title：Biophysical journal

authors： Schurr JM

更新日期：1970-08-01 00:00:00

abstract：:A nitroxide spin label attached to the C-terminus of the channel forming peptide alamethicin produces an enhancement of the nuclear spin-lattice relaxation rates of peptide protons as a result of both intermolecular and intramolecular magnetic dipole-dipole interactions. The intermolecular contribution provides eviden...

journal_title：Biophysical journal

authors： North CL,Franklin JC,Bryant RG,Cafiso DS

更新日期：1994-11-01 00:00:00

abstract：:The mathematical model of steady-state iron kinetics in the human presented here extends the use of radioactive tracer observations in the determination of erythron maturative and proliferative behavior. Functions descriptive of that behavior appear explicitly in the model together with functions customarily measured ...

journal_title：Biophysical journal

authors： Nooney GC

更新日期：1965-11-01 00:00:00

abstract：:A mode of internal motion of single tryptophan, Trp 86, of Streptomyces subtilisin inhibitor, was analyzed from its time-resolved fluorescence. The intensity and anisotropy decays of Trp 86 were measured in the picosecond range. These decays were analyzed with theoretical expressions derived assuming that the indole r...

journal_title：Biophysical journal

authors： Tanaka F,Tamai N,Mataga N,Tonomura B,Hiromi K

更新日期：1994-08-01 00:00:00

abstract：:We describe two enhancements of the planar bilayer recording method which enable low-noise recordings of single-channel currents activated by voltage steps in planar bilayers formed on apertures in partitions separating two open chambers. First, we have refined a simple and effective procedure for making small bilayer...

journal_title：Biophysical journal

authors： Wonderlin WF,Finkel A,French RJ

更新日期：1990-08-01 00:00:00

abstract：:K(ATP) channels are comprised of a pore-forming protein, Kir6.x, and the sulfonylurea receptor, SURx. Interaction of adenine nucleotides with Kir6.2 positively charged amino acids such as K185 and R201 on the C-terminus causes channel closure. Substitution of these amino acids with other positively charged residues ha...

journal_title：Biophysical journal

authors： Ribalet B,John SA,Weiss JN

更新日期：2003-01-01 00:00:00

abstract：:Metallic nanoparticles have opened the possibility of imaging, tracking, and manipulating biological samples without time limitations. Their low photoluminescence quantum yield, however, makes them hard to detect under high background conditions. In this study we show that it is possible to image gold nanorods by dete...

journal_title：Biophysical journal

authors： Carattino A,Keizer VIP,Schaaf MJM,Orrit M

更新日期：2016-12-06 00:00:00

abstract：:Biological membranes composed of lipids and proteins are in contact with electrolytes like aqueous NaCl solutions. Based on molecular dynamics studies it is widely believed that Na(+) ions specifically bind to 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine (POPC) membranes, whereas Cl(-) ions stay in solution. Here,...

journal_title：Biophysical journal

authors： Knecht V,Klasczyk B

更新日期：2013-02-19 00:00:00

abstract：:The aim of this study was to gain insight into how interactions between proteins and lipids in membranes are sensed at the protein-lipid interface. As a probe to analyze this interface, we used deuterium-labeled acyl chains that were covalently linked to a model transmembrane peptide. First, a perdeuterated palmitoyl ...

journal_title：Biophysical journal

authors： Nyholm TK,van Duyl B,Rijkers DT,Liskamp RM,Killian JA

更新日期：2011-10-19 00:00:00