Abstract:
:Surface and subsurface dynamics of Rat Basophilic Leukemia cells, a model system of stimulated secretion, were imaged using Scanning Force Microscopy (SFM) at a rate of 50-60 s/image. Cytoskeletal elements and organelles were tracked within quiescent cells and those activated after IgE receptor crosslinking. In addition, surface waves were observed moving within the plasma membrane. The structures seen in quiescent and activated cells can be correlated with those seen in electron micrographs and topographic SFM images of fixed detergent-extracted cells. Furthermore, images of the detergent-extracted nuclei reveal the presence of numerous nuclear pore complexes. High-magnification images of the nuclear pore complexes show evidence of subunit structure and exhibit dimensions consistent with those reported previously using electron microscopy. The behavior and overall change in morphology of cells observed during activation was consistent with that observed under similar conditions with Differential Interference Contrast microscopy. This study demonstrates that SFM, unlike other techniques, can be used to provide high-resolution information in both fixed and living cells.
journal_name
Biophys Jjournal_title
Biophysical journalauthors
Braunstein D,Spudich Adoi
10.1016/S0006-3495(94)80964-6subject
Has Abstractpub_date
1994-05-01 00:00:00pages
1717-25issue
5eissn
0006-3495issn
1542-0086pii
S0006-3495(94)80964-6journal_volume
66pub_type
杂志文章abstract::The excitation of the squid giant axon that had been perfused intra- and extracellularly with solutions containing a high concentration of glycerol could be observed below 0 degree C. The action potential could be elicited at normal strengths of electrical stimuli. The time-course of the action potential was slowed, w...
journal_title:Biophysical journal
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journal_title:Biophysical journal
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更新日期:1995-06-01 00:00:00
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journal_title:Biophysical journal
pub_type: 杂志文章
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更新日期:1995-03-01 00:00:00
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pub_type: 杂志文章
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更新日期:2014-05-06 00:00:00
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journal_title:Biophysical journal
pub_type: 杂志文章
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