Abstract:
:Gaining information about receptor profiles on cells, and subsequently finding the most efficient ligands for these signaling receptors, remain challenging tasks in stem cell and cancer research as well as drug development. We introduce a live-cell method with great potential in both screening for surface receptors and analysing binding forces of different ligands. The technique is based on the molecular force assay, a parallel-format, high-throughput experiment on a single-molecule level. On human red blood cells, we demonstrate the detection of the interaction of N-acetyl-α-D-galactosaminyl residues with the lectin helix pomatia agglutinine and of the CD47 receptor with its antibody. The measurements are performed under nearly physiological conditions and still provide a highly specific binding signal. Moreover, with a detailed comparative force analysis on two cell types with different morphology, we show that our method even allows the determination of a DNA force equivalent for the interaction of the CD47 receptor and its antibody.
journal_name
Biophys Jjournal_title
Biophysical journalauthors
Wienken U,Gaub HEdoi
10.1016/j.bpj.2013.10.013subject
Has Abstractpub_date
2013-12-17 00:00:00pages
2687-94issue
12eissn
0006-3495issn
1542-0086pii
S0006-3495(13)01144-2journal_volume
105pub_type
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