Abstract:
:A phospholipid chelate complexed with ytterbium (DMPE-DTPA:Yb3+) is shown to be readily incorporated into a model membrane system, which may then be aligned in a magnetic field such that the average bilayer normal lies along the field. This so-called positively ordered smectic phase, whose lipids consist of less than 1% DMPE-DTPA:Yb3+, is ideally suited to structural studies of membrane proteins by solid-state NMR, low-angle diffraction, and spectroscopic techniques that require oriented samples. The chelate, 1,2-dimyristoyl-sn-glycero-3-phosphoethanolamine diethylenetriaminepentaacetic acid, which strongly binds the lanthanide ions and serves to orient the membrane in a magnetic field, prevents direct lanthanide-protein interactions and significantly reduces paramagnetic shifts and line broadening. Similar low-spin lanthanide chelates may have applications in field-ordered solution NMR studies of water-soluble proteins and in the design of new magnetically aligned liquid crystalline phases.
journal_name
Biophys Jjournal_title
Biophysical journalauthors
Prosser RS,Volkov VB,Shiyanovskaya IVdoi
10.1016/S0006-3495(98)77659-3subject
Has Abstractpub_date
1998-11-01 00:00:00pages
2163-9issue
5eissn
0006-3495issn
1542-0086pii
S0006-3495(98)77659-3journal_volume
75pub_type
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