Abstract：
:In this review, we focus on the early events in the process of fibroblast spreading on fibronectin matrices of different rigidities. We present a focused position piece that illustrates the many different tests that a cell makes of its environment before it establishes mature matrix adhesions. When a fibroblast is placed on fibronectin-coated glass surfaces at 37°C, it typically spreads and polarizes within 20-40 min primarily through αvβ3 integrin binding to fibronectin. In that short period, the cell goes through three major phases that involve binding, integrin activation, spreading, and mechanical testing of the surface. The advantage of using the model system of cell spreading from the unattached state is that it is highly reproducible and the stages that the cell undergoes can thus be studied in a highly quantitative manner, in both space and time. The mechanical and biochemical parameters that matter in this example are often surprising because of both the large number of tests that occur and the precision of the tests. We discuss our current understanding of those tests, the decision tree that is involved in this process, and an extension to the behavior of the cells at longer time periods when mature adhesions develop. Because many other matrices and integrins are involved in cell-matrix adhesion, this model system gives us a limited view of a subset of cellular behaviors that can occur. However, by defining one cellular process at a molecular level, we know more of what to expect when defining other processes. Because each cellular process will involve some different proteins, a molecular understanding of multiple functions operating within a given cell can lead to strategies to selectively block a function.
journal_name
Biophys Jjournal_title
Biophysical journalauthors
Wolfenson H,Iskratsch T,Sheetz MPdoi
10.1016/j.bpj.2014.10.041subject
Has Abstractpub_date
2014-12-02 00:00:00pages
2508-14issue
11eissn
0006-3495issn
1542-0086pii
S0006-3495(14)01127-8journal_volume
107pub_type
杂志文章,评审abstract：:Crystals have been grown of "sperm whale" myoglobin produced in Escherichia coli from a synthetic gene and the structure has been solved to 1.9 A resolution. Because of a remaining initiator methionine, this protein crystallizes in a different space group from native sperm whale myoglobin. The three-dimensional struct...
journal_title：Biophysical journal
pub_type： 杂志文章
doi：10.1016/S0006-3495(90)82540-6
更新日期：1990-02-01 00:00:00
abstract：:We report noncontact optical measurement of fast transient structural changes in the crustacean nerve during action potential propagation without the need for exogenous chemicals or reflection coatings. The technique, spectral domain optical coherence tomography, provides real-time cross-sectional images of the nerve ...
journal_title：Biophysical journal
pub_type： 杂志文章
doi：10.1529/biophysj.106.091298
更新日期：2007-08-15 00:00:00
abstract：:Beat-to-beat alternation of the action potential duration (APD) in paced cardiac cells has been linked to the onset of lethal arrhythmias. Both experimental and theoretical studies have shown that alternans at the single cell level can be caused by unstable membrane voltage (V(m)) dynamics linked to steep APD-restitut...
journal_title：Biophysical journal
pub_type： 杂志文章
doi：10.1529/biophysj.106.100982
更新日期：2007-02-15 00:00:00
abstract：:Enzyme function depends on specific conformational motions. We show that the temperature dependence of enzyme kinetic parameters can provide insight into these functionally relevant motions. While investigating the catalytic properties of IPMDH from Escherichia coli, we found that its catalytic efficiency (k(cat)/K(M,...
journal_title：Biophysical journal
pub_type： 杂志文章
doi：10.1016/j.bpj.2009.04.014
更新日期：2009-06-17 00:00:00
abstract：:The effect of peptides, corresponding to sequences in the skeletal muscle dihydropyridine receptor II-III loop, on Ca(2+) release from sarcoplasmic reticulum (SR) and on ryanodine receptor (RyR) calcium release channels have been compared in preparations from normal and malignant hyperthermia (MH)-susceptible pigs. Pe...
journal_title：Biophysical journal
pub_type： 杂志文章
doi：10.1016/S0006-3495(01)76147-4
更新日期：2001-04-01 00:00:00
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journal_title：Biophysical journal
pub_type： 杂志文章
doi：10.1016/S0006-3495(92)81950-1
更新日期：1992-06-01 00:00:00
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journal_title：Biophysical journal
pub_type： 杂志文章
doi：10.1016/j.bpj.2020.09.002
更新日期：2020-10-20 00:00:00
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journal_title：Biophysical journal
pub_type： 杂志文章
doi：10.1016/S0006-3495(03)74532-9
更新日期：2003-08-01 00:00:00
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journal_title：Biophysical journal
pub_type： 杂志文章
doi：10.1529/biophysj.106.087072
更新日期：2006-11-15 00:00:00
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journal_title：Biophysical journal
pub_type： 杂志文章
doi：10.1016/s0006-3495(01)75726-8
更新日期：2001-08-01 00:00:00
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journal_title：Biophysical journal
pub_type： 杂志文章
doi：10.1016/S0006-3495(70)86355-X
更新日期：1970-11-01 00:00:00
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journal_title：Biophysical journal
pub_type： 杂志文章
doi：10.1016/S0006-3495(94)80765-9
更新日期：1994-01-01 00:00:00
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journal_title：Biophysical journal
pub_type： 杂志文章
doi：10.1529/biophysj.106.090555
更新日期：2007-03-01 00:00:00
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journal_title：Biophysical journal
pub_type： 杂志文章
doi：10.1016/S0006-3495(76)85720-7
更新日期：1976-06-01 00:00:00
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journal_title：Biophysical journal
pub_type： 杂志文章
doi：10.1016/j.bpj.2020.08.025
更新日期：2020-10-06 00:00:00
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journal_title：Biophysical journal
pub_type： 杂志文章
doi：10.1016/j.bpj.2010.08.023
更新日期：2010-10-20 00:00:00
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journal_title：Biophysical journal
pub_type： 杂志文章
doi：10.1529/biophysj.107.112904
更新日期：2008-04-01 00:00:00
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journal_title：Biophysical journal
pub_type： 杂志文章
doi：10.1016/S0006-3495(03)74490-7
更新日期：2003-07-01 00:00:00
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journal_title：Biophysical journal
pub_type： 杂志文章
doi：10.1016/j.bpj.2010.02.036
更新日期：2010-06-02 00:00:00
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journal_title：Biophysical journal
pub_type： 杂志文章
doi：10.1016/s0006-3495(65)86707-8
更新日期：1965-03-01 00:00:00
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journal_title：Biophysical journal
pub_type： 杂志文章
doi：10.1016/S0006-3495(94)80715-5
更新日期：1994-12-01 00:00:00
abstract：:A Green's function approach is used in constructing a dynamic model of a semi-infinite length of the DNA homopolymer B poly(d) . poly(d). Considerable attention is focused on the hydrogen bond stretching close to the terminus. A melting (or breathing) coordinate (M) is defined as an average over the three linking hydr...
journal_title：Biophysical journal
pub_type： 杂志文章
doi：10.1016/S0006-3495(81)84789-3
更新日期：1981-08-01 00:00:00
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journal_title：Biophysical journal
pub_type： 杂志文章
doi：10.1529/biophysj.107.125369
更新日期：2009-02-01 00:00:00
abstract：:The plasma membrane of cells has a complex architecture based on the bidimensional liquid-crystalline bilayer arrangement of phospho- and sphingolipids, which in turn embeds several proteins and is connected to the cytoskeleton. Several studies highlight the spatial membrane organization into more ordered (Lo or lipid...
journal_title：Biophysical journal
pub_type： 杂志文章
doi：10.1016/j.bpj.2018.11.3141
更新日期：2019-02-05 00:00:00
abstract：:The kinetics of force production in chemically skinned trabeculae from the guinea pig were studied by laser photolysis of caged ATP in the presence of Ca2+. Preincubation of the tissue during rigor with the enzyme apyrase was used to reduce the population of MgADP-bound cross-bridges (Martin and Barsotti, 1994). In un...
journal_title：Biophysical journal
pub_type： 杂志文章
doi：10.1016/S0006-3495(94)80676-9
更新日期：1994-11-01 00:00:00
abstract：:Osmotic stress in the 0.5-5 x 10(6) dyne/cm2 range was used to perturb the hydration of actin-myosin-ATP intermediates during steady-state hydrolysis. Polyethylene glycol (PEG) (1000 to 4000 Da), in the 1 to 10 wt% range, which does not cause protein precipitation, did not significantly affect the apparent KM or the V...
journal_title：Biophysical journal
pub_type： 杂志文章
doi：10.1016/S0006-3495(96)79852-1
更新日期：1996-06-01 00:00:00
abstract：:The effects of different cations on the hydrodynamic radius (RH) of a 48-bp synthetic DNA are measured by time-resolved fluorescence polarization anisotropy of intercalated ethidium. Relative statistical errors in RH are only approximately 1%. With increasing cation concentration, Na+ causes a small decrease in RH, Cs...
journal_title：Biophysical journal
pub_type： 杂志文章
doi：10.1016/S0006-3495(94)80481-3
更新日期：1994-07-01 00:00:00
abstract：:The strength of receptor-mediated cell adhesion is directly controlled by the mechanism of cohesive failure between the cell surface and underlying substrate. Unbinding can occur either at the locus of the specific bond or within the bilayer, which results in tearing the hydrophobic anchors from the membrane interior....
journal_title：Biophysical journal
pub_type： 杂志文章
doi：10.1016/S0006-3495(95)79990-8
更新日期：1995-09-01 00:00:00
abstract：:A nitroxide spin label attached to the C-terminus of the channel forming peptide alamethicin produces an enhancement of the nuclear spin-lattice relaxation rates of peptide protons as a result of both intermolecular and intramolecular magnetic dipole-dipole interactions. The intermolecular contribution provides eviden...
journal_title：Biophysical journal
pub_type： 杂志文章
doi：10.1016/S0006-3495(94)80667-8
更新日期：1994-11-01 00:00:00
abstract：:Halobacterium halobium contains at least three retinal-containing pigments: bacteriorhodopsin, halorhodopsin, and a third rhodopsin-like pigment (tR) absorbing at approximately 590 nm, tR590. Illumination of tR590 gives rise to a very long-lived blue absorbing photoproduct, tR370. Using high-performance liquid chromat...
journal_title：Biophysical journal
pub_type： 杂志文章
doi：10.1016/S0006-3495(85)83969-2
更新日期：1985-05-01 00:00:00
