Unusual Isothermal Hysteresis in DNA i-Motif pH Transitions: A Study of the RAD17 Promoter Sequence.

abstract：:Association of transmembrane (TM) helices is facilitated by the close packing of small residues present along the amino-acid sequence. Extensive studies have established the role of such small residue motifs (GxxxG) in the dimerization of Glycophorin A (GpA) and helped to elucidate the association of TM domains in the...

journal_title：Biophysical journal

authors： Prakash A,Janosi L,Doxastakis M

更新日期：2010-12-01 00:00:00

abstract：:A promising strategy to control the aggregation of the Alzheimer's Aβ peptide in the brain is the clearance of Aβ from the central nervous system into the peripheral blood plasma. Among plasma proteins, human serum albumin plays a critical role in the Aβ clearance to the peripheral sink by binding to Aβ oligomers and ...

journal_title：Biophysical journal

authors： Milojevic J,Melacini G

更新日期：2011-01-05 00:00:00

abstract：:Cubic insulin crystals contain a binding site for monovalent cations in a cavity of the crystal dyad in which the bound cation is ligated by protein atomic dipoles and water molecules. These types of interaction are analogous to interactions that occur in small cation-selective carrier and channel molecules. X-ray dif...

journal_title：Biophysical journal

authors： Badger J,Kapulsky A,Gursky O,Bhyravbhatla B,Caspar DL

更新日期：1994-02-01 00:00:00

abstract：:Subdenaturing concentrations of guanidine hydrochloride (GdnHCl) stabilize proteins. For ferrocytochrome c the stabilization is detected at subglobal level with no measured change in global stability. These deductions are made by comparing observed rates of thermally driven ferrocytochrome cHCO reactions with global u...

journal_title：Biophysical journal

authors： Kumar R,Prabhu NP,Yadaiah M,Bhuyan AK

更新日期：2004-10-01 00:00:00

abstract：:Protein-mediated DNA looping, such as that induced by the lactose repressor (LacI) of Escherichia coli, is a well-known gene regulation mechanism. Although researchers have given considerable attention to DNA looping by LacI, many unanswered questions about this mechanism, including the role of protein flexibility, re...

journal_title：Biophysical journal

authors： Hirsh AD,Lillian TD,Lionberger TA,Perkins NC

更新日期：2011-08-03 00:00:00

abstract：:We have measured the electronic circular dichroism (ECD) of the ferri- and ferro-states of several natural cytochrome c derivatives (horse heart, chicken, bovine, and yeast) and the Y67F mutant of yeast in the region between 300 and 750 nm. Thus, we recorded the ECD of the B- and Q-band region as well as the charge-tr...

journal_title：Biophysical journal

authors： Dragomir I,Hagarman A,Wallace C,Schweitzer-Stenner R

更新日期：2007-02-01 00:00:00

abstract：:The M2 protein from the influenza A virus forms a proton channel in the virion that is essential for infection. This tetrameric protein appears to form a four-helix bundle spanning the viral membrane. Here the solid-state NMR method, 2D polarization inversion spin exchange at magic angle (PISEMA), has been used to obt...

journal_title：Biophysical journal

authors： Song Z,Kovacs FA,Wang J,Denny JK,Shekar SC,Quine JR,Cross TA

更新日期：2000-08-01 00:00:00

abstract：:Lipid mixing between vesicles functionalized with SNAREs and the cytosolic C2AB domain of synaptotagmin-1 recapitulates the basic Ca(2+) dependence of neuronal exocytosis. However, in the conventional ensemble lipid mixing assays it is not possible to discriminate whether Ca(2+) accelerates the docking or the fusion o...

journal_title：Biophysical journal

authors： Christensen SM,Mortensen MW,Stamou DG

更新日期：2011-02-16 00:00:00

abstract：:An analysis is presented of the complex anisotropy behavior of trans-parinaric acid in single component DEPC lipid bilayers. It is shown that a model involving two species with distinct lifetime and motional behavior is required, and is adequate, to explain the observed data. In particular, the observed increase in th...

journal_title：Biophysical journal

authors： Ruggiero A,Hudson B

更新日期：1989-06-01 00:00:00

abstract：:We studied the photocurrents of a cyanobacterial rhodopsin Gloeobacter violaceus (GR) in Xenopus laevis oocytes and HEK-293 cells. This protein is a light-driven proton pump with striking similarities to marine proteorhodopsins, including the D121-H87 cluster of the retinal Schiff base counterion and a glutamate at po...

journal_title：Biophysical journal

authors： Vogt A,Wietek J,Hegemann P

更新日期：2013-11-05 00:00:00

abstract：:In permeabilized single fibers of fast (psoas) and slow (soleus) muscle from the rabbit, the effect of pH on isometric myofibrillar ATPase activity and force was studied at 15 degrees C, in the pH range 6.4-7.9. ATPase activity was measured photometrically by enzymatic coupling of the regeneration of ATP to the oxidat...

journal_title：Biophysical journal

authors： Potma EJ,van Graas IA,Stienen GJ

更新日期：1994-12-01 00:00:00

abstract：:De novo formation of cell pairs was used to examine the gating properties of single gap junction channels. Two separate cells of an insect cell line (clone C6/36, derived from the mosquito Aedes albopictus) were pushed against each other to provoke formation of gap junction channels. A dual voltage-clamp method was us...

journal_title：Biophysical journal

authors： Bukauskas FF,Weingart R

更新日期：1994-08-01 00:00:00

abstract：:Presented here is a quantitative model of the wrapping and unwrapping of the DNA around the histone core of the nucleosome that suggests a mechanism by which this transition can be controlled: alteration of the charge state of the globular histone core. The mechanism is relevant to several classes of posttranslational...

journal_title：Biophysical journal

authors： Fenley AT,Adams DA,Onufriev AV

更新日期：2010-09-08 00:00:00

abstract：:A phenomenological theory for contracting muscle based on irreversible thermodynamics and the sliding filament theory is developed. The individual cross bridges, considered as subunits, are viewed as linear energy converters with constant transport coefficients. With this view of the subunits, phenomenological equatio...

journal_title：Biophysical journal

authors： Bornhorst WJ,Minardi JE

更新日期：1970-02-01 00:00:00

abstract：:Encapsulation of proteins within lipid inverse bicontinuous cubic phases (Q2) has been widely studied for many applications, such as protein crystallization or drug delivery of proteins for food and pharmaceutical purposes. However, the use of the lipid sponge (L3) phase for encapsulation of proteins has not yet been ...

journal_title：Biophysical journal

authors： Valldeperas M,Talaikis M,Dhayal SK,Velička M,Barauskas J,Niaura G,Nylander T

更新日期：2019-09-03 00:00:00

abstract：:The characteristic equatorial X-ray pattern from a relaxed vertebrate skeletal muscle changes when the muscle is activated. In particular, there is a simultaneous decrease in the intensity of the first reflection (I10) and increase in the intensity of the second (I11). This observed change is almost reciprocal. When c...

journal_title：Biophysical journal

authors： Lymn RW

更新日期：1978-01-01 00:00:00

abstract：:The structural basis of the wide variability of the physical properties of fibrin clots and the process of assembly of the clot were investigated by electron microscopy of fibers formed under various ionic conditions. In addition, highly specific proteolytic enzymes from different snake venoms were used to remove sele...

journal_title：Biophysical journal

authors： Weisel JW

更新日期：1986-12-01 00:00:00

abstract：:Nef is an HIV-1 accessory protein that directly contributes to AIDS progression. Nef is myristoylated on the N-terminus, associates with membranes, and may undergo a transition from a solution conformation to a membrane-associated conformation. It has been hypothesized that conformational rearrangement enables membran...

journal_title：Biophysical journal

authors： Kent MS,Murton JK,Sasaki DY,Satija S,Akgun B,Nanda H,Curtis JE,Majewski J,Morgan CR,Engen JR

更新日期：2010-09-22 00:00:00

abstract：:Atomic force microscopy (AFM) has become an important tool for quantifying mechanical properties of biological materials ranging from single molecules to cells and tissues. Current AFM techniques for measuring elastic and viscoelastic properties of whole cells are based on indentation of cells firmly adhered to a subs...

journal_title：Biophysical journal

authors： Rosenbluth MJ,Lam WA,Fletcher DA

更新日期：2006-04-15 00:00:00

abstract：:Quasilongitudinal sound velocities and the second-order elastic moduli of tetragonal hen egg-white lysozyme crystals were determined as a function of relative humidity (RH) by Brillouin scattering. In hydrated crystals the measured sound velocities in the [110] plane vary between 2.12 +/- 0.03 km/s along the [001] dir...

journal_title：Biophysical journal

authors： Speziale S,Jiang F,Caylor CL,Kriminski S,Zha CS,Thorne RE,Duffy TS

更新日期：2003-11-01 00:00:00

abstract：:The effects of internally and externally applied sulfhydryl reagents on the bioelectric properties of the giant axon of the squid Loligo pealeii and Dosidicus gigas were studied. Cysteine-HCl (400 mM, pH 7.3) was used to remove axoplasm from the perfusion channel. Oxidizing agents (1 to 60 mM) tended to increase the d...

journal_title：Biophysical journal

authors： Huneeus-Cox F,Fernandez HL,Smith BH

更新日期：1966-09-01 00:00:00

abstract：:A model for the action of vinblastine (VLB) on cells multiplying exponentially in vivo with a generation time, T(G), has been derived. It is based on the assumption that cells attempting to pass through mitosis in the presence of VLB lose their proliferative capacity and that this lethal effect occurs only when the ce...

journal_title：Biophysical journal

authors： Valeriote FA,Bruce WR,Meeker BE

更新日期：1966-03-01 00:00:00

abstract：:The effects of quinidine on single inward rectifier K channels were investigated in cell-attached patches with 4.5 mM pipette potassium concentrations. Under these conditions, the single-channel slope conductance of the predominant conductance level of the inward rectifier channels was 3.9 +/- 0.3 pS at membrane poten...

journal_title：Biophysical journal

authors： Balser JR,Roden DM,Bennett PB

更新日期：1991-01-01 00:00:00

abstract：:Reverberating spontaneous synchronized brain activity is believed to play an important role in neural information processing. Whether and how external stimuli can influence this spontaneous activity is poorly understood. Because periodic synchronized network activity is also prominent in in vitro neuronal cultures, we...

journal_title：Biophysical journal

authors： Vajda I,van Pelt J,Wolters P,Chiappalone M,Martinoia S,van Someren E,van Ooyen A

更新日期：2008-06-01 00:00:00

abstract：:We study theoretically the feasibility of using transverse electronic transport within a nanopore for rapid DNA sequencing. Specifically, we examine the effects of the environment and detection probes on the distinguishability of the DNA bases. We find that the intrinsic measurement bandwidth of the electrodes helps t...

journal_title：Biophysical journal

authors： Lagerqvist J,Zwolak M,Di Ventra M

更新日期：2007-10-01 00:00:00

abstract：:We report that long-chain poly-L-glutamine forms cation-selective channels when incorporated into artificial planar lipid bilayer membranes. The channel was permeable to alkali cations and H(+) ions and virtually impermeable to anions; the selectivity sequence based on the single-channel conductance was H(+) > Cs(+) >...

journal_title：Biophysical journal

authors： Monoi H,Futaki S,Kugimiya S,Minakata H,Yoshihara K

更新日期：2000-06-01 00:00:00

abstract：:Regulation of the F-actin severing activity of gelsolin by Ca2+ has been investigated under physiologic ionic conditions. Tryptophan fluorescence intensity measurements indicate that gelsolin contains at least two Ca2+ binding sites with affinities of 2.5 x 10(7) M-1 and 1.5 x 10(5) M-1. At F-actin and gelsolin concen...

journal_title：Biophysical journal

authors： Kinosian HJ,Newman J,Lincoln B,Selden LA,Gershman LC,Estes JE

更新日期：1998-12-01 00:00:00

abstract：:The canonical mass balance relation derived for the central core model of the renal medulla is extended to medullary models in which an arbitrary assemblage of renal tubules and vascular capillaries exchange with each other both directly and via the medullary interstitium and in which not all of the vascular loops or ...

journal_title：Biophysical journal

authors： Stephenson JL

更新日期：1976-11-01 00:00:00

abstract：:Nonequilibrium molecular dynamics (NEMD) computer simulations are used to calculated the bulk modulus for a dimyristoylphosphatidylcholine bilayer. A methodology is developed whereby NEMD can be effectively used to calculate material properties for complex systems that undergo long time-scale conformational changes. I...

journal_title：Biophysical journal

authors： Ayton G,Smondyrev AM,Bardenhagen SG,McMurtry P,Voth GA

更新日期：2002-03-01 00:00:00

abstract：:Moesin, a protein of the ezrin, radixin, and moesin family, which links the plasma membrane to the cytoskeleton, is involved in multiple physiological and pathological processes, including viral budding and infection. Its interaction with the plasma membrane occurs via a key phosphoinositide, the phosphatidyl(4,5)inos...

journal_title：Biophysical journal

authors： Lubart Q,Vitet H,Dalonneau F,Le Roy A,Kowalski M,Lourdin M,Ebel C,Weidenhaupt M,Picart C

更新日期：2018-01-09 00:00:00