P-chloromercuribenzene sulfonate blocks and reverses the effect of amiloride on sodium transport across rabbit colon in vitro.

abstract：:We introduce the use of microfabrication techniques to construct on a silicon wafer a synthetic capillary bed with 2.5- to 4-micron (mu)-wide channels. Establishment of a fluid pressure gradient allowed us to observe simultaneously using optical microscopy hundreds of cells flowing through the bed at physiological spe...

journal_title：Biophysical journal

authors： Brody JP,Han Y,Austin RH,Bitensky M

更新日期：1995-06-01 00:00:00

abstract：:Chinese hamster ovary cells were irradiated with 100-5,000 rads of X-rays and inhibition of the initiation of replicons after irradiation was demonstrated by analyzing nascent DNA sedimented in alkaline sucrose gradients. The renaturation kinetics of DNA synthesized during 60 min of incubation after irradiation was co...

journal_title：Biophysical journal

authors： Mattern MR,Painter RB

更新日期：1977-08-01 00:00:00

abstract：:The increasingly widespread use of parametric mathematical models to describe biological systems means that the ability to infer model parameters is of great importance. In this study, we consider parameter inferability in nonlinear ordinary differential equation models that undergo a bifurcation, focusing on a simple...

journal_title：Biophysical journal

authors： Kirk PD,Toni T,Stumpf MP

更新日期：2008-07-01 00:00:00

abstract：:The change in capacity of squid axon membrane during hyper- and depolarizations was investigated in the absence of ionic currents after the membrane was treated with pronase. In the presence of the inactivation process (h parameter), failure to observe the gating current in the frequency domain was attributed to the r...

journal_title：Biophysical journal

authors： Takashima S

更新日期：1978-04-01 00:00:00

abstract：:We study the folding mechanism of a triple beta-strand WW domain from the Formin binding protein 28 (FBP28) at atomic resolution with explicit water model using replica exchange molecular dynamics computer simulations. Extended sampling over a wide range of temperatures to obtain the free energy, enthalpy, and entropy...

journal_title：Biophysical journal

authors： Mu Y,Nordenskiöld L,Tam JP

更新日期：2006-06-01 00:00:00

abstract：:Gene regulation involves a hierarchy of events that extend from specific protein-DNA interactions to the combinatorial assembly of nucleoprotein complexes. The effects of DNA sequence on these processes have typically been studied based either on its quantitative connection with single-domain binding free energies or ...

journal_title：Biophysical journal

authors： Vilar JM

更新日期：2010-10-20 00:00:00

abstract：:Despite the importance of magnetic properties of biological samples for biomagnetism and related fields, the exact magnetic susceptibilities of most biological samples in their physiological conditions are still unknown. Here we used superconducting quantum interferometer device to detect the magnetic properties of no...

journal_title：Biophysical journal

authors： Tao Q,Zhang L,Han X,Chen H,Ji X,Zhang X

更新日期：2020-02-04 00:00:00

abstract：:A 15-ns molecular dynamics (MD) simulation of the fully hydrated dimyristoylphosphatidylcholine-cholesterol (DMPC-Chol) bilayer in the liquid-crystalline state was carried out to investigate the effect of Chol on the hydrocarbon chain region of the bilayer. The last 8-ns fragment of the generated trajectory was used f...

journal_title：Biophysical journal

authors： Róg T,Pasenkiewicz-Gierula M

更新日期：2001-10-01 00:00:00

abstract：:KATP channels assemble from four regulatory SUR1 and four pore-forming Kir6.2 subunits. At the single-channel current level, ATP-dependent gating transitions between the active burst and the inactive interburst conformations underlie inhibition of the KATP channel by intracellular ATP. Previously, we identified a slow...

journal_title：Biophysical journal

authors： Drain P,Geng X,Li L

更新日期：2004-04-01 00:00:00

abstract：:Differential scanning calorimetry provides a new window into the plasma proteome. Plasma from normal individuals yields a characteristic, reproducible thermogram that appears to represent the weighted sum of denaturation profiles of the most abundant constituent plasma proteins. Plasma from diseased individuals yields...

journal_title：Biophysical journal

authors： Garbett NC,Miller JJ,Jenson AB,Chaires JB

更新日期：2008-02-15 00:00:00

abstract：:An equation is derived, which connects two functions P(r) and g(x), the first being related to the scattering intensity by a simple transformation, the second to the elctron density distribution of a spherically symmetric structure. This relation seems to be a suitable starting point for an analysis of shell structure...

journal_title：Biophysical journal

authors： Weick D

更新日期：1974-03-01 00:00:00

abstract：:The early events in halorhodopsin after light excitation are studied with picosecond time resolution. Absorption and fluorescence measurements show that the electronically excited state of the incorporated retinal has a lifetime of 5 ps. Within that time a red-shifted photoproduct is formed that remains stable for at ...

journal_title：Biophysical journal

authors： Polland HJ,Franz MA,Zinth W,Kaiser W,Hegemann P,Oesterhelt D

更新日期：1985-01-01 00:00:00

abstract：:We examined the effects of brief current pulses on the pacemaker oscillations of the Purkinje fiber using the model of McAllister , Noble, and Tsien (1975. J. Physiol. [Lond.]. 251:1-57). This model was used to construct phase-response curves for brief electric stimuli to find "black holes," where rhythmic activity of...

journal_title：Biophysical journal

authors： Chay TR,Lee YS

更新日期：1984-04-01 00:00:00

abstract：:The theoretical relationships between the fluorescence and photochemical yields of PS II and the fraction of open reaction centers are examined in a general model endowed with the following features: i) a homogeneous, infinite PS II domain; ii) exciton-radical-pair equilibrium; and iii) different rates of exciton tran...

journal_title：Biophysical journal

authors： Lavergne J,Trissl HW

更新日期：1995-06-01 00:00:00

abstract：:Isopycnic banding of Chinese hamster line CHO cells in Ficoll gradients shows that a population in balanced, exponential growth is very homogeneous with respect to density, the coefficient of variation of the density distribution spectrum being less than 5% of the mean reduced density (i.e. density minus one). Similar...

journal_title：Biophysical journal

authors： Anderson EC,Petersen DF,Tobey RA

更新日期：1970-07-01 00:00:00

abstract：:The retinal Na+:Ca2+, K+exchanger cDNA was transiently expressed in human embryonic kidney (HEK 293) cells by transfection with plasmid DNA. The correct targeting of the expressed protein to the plasma membrane was confirmed by immunocytochemistry. The reverse exchange offrent (Ca2+ imported per Na+ extruded) was meas...

journal_title：Biophysical journal

authors： Navanglone A,Rispoli G,Gabellini N,Carafoli E

更新日期：1997-07-01 00:00:00

abstract：:The adult form of the nicotinic acetylcholine receptor (AChR) consists of five subunits (alpha(2)betaepsilondelta), each having four transmembrane domains (M1-M4). The atomic model of the nicotinic acetylcholine receptor shows that the pore-lining M2 domains make no extensive contacts with the rest of the transmembran...

journal_title：Biophysical journal

authors： Corradi J,Spitzmaul G,De Rosa MJ,Costabel M,Bouzat C

更新日期：2007-01-01 00:00:00

abstract：:The voltage dependence of light-induced proton pumping was studied with bacteriorhodopsin (bR) from Halobacterium salinarum, expressed in the plasma membrane of oocytes from Xenopus laevis in the range -160 mV to +60 mV at different light intensities. Depending on the applied field, the quenching effect by blue light,...

journal_title：Biophysical journal

authors： Nagel G,Kelety B,Möckel B,Büldt G,Bamberg E

更新日期：1998-01-01 00:00:00

abstract：:Understanding the fundamentals of genome packaging in viral capsids is important for finding effective antiviral strategies and for utilizing benign viral particles for gene therapy. While the structure of encapsidated genomic materials has been routinely characterized with experimental techniques such as cryo-electro...

journal_title：Biophysical journal

authors： Kim J,Wu J

更新日期：2015-10-20 00:00:00

abstract：:Collagenase H (ColH) from Clostridium histolyticum is a multimodular protein composed of a collagenase module (activator and peptidase domains), two polycystic kidney disease-like domains, and a collagen-binding domain. The interdomain conformation and its changes are very important for understanding the functions of ...

journal_title：Biophysical journal

authors： Ohbayashi N,Matsumoto T,Shima H,Goto M,Watanabe K,Yamano A,Katoh Y,Igarashi K,Yamagata Y,Murayama K

更新日期：2013-04-02 00:00:00

abstract：:We report a simple method to obtain stable asymmetric giant unilamellar vesicles (GUVs). Fluorescence correlation spectroscopy was used to quantitatively characterize vesicle properties. After brain sphingomyelin (bSM) was exchanged into dioleoylphosphatidylcholine (DOPC) GUVs, lateral diffusion in the bSM-containing ...

journal_title：Biophysical journal

authors： Chiantia S,Schwille P,Klymchenko AS,London E

更新日期：2011-01-05 00:00:00

abstract：:The influence of the amino acid residues surrounding the flavin ring in the flavodoxin of the cyanobacterium Anabaena PCC 7119 on the electron spin density distribution of the flavin semiquinone was examined in mutants of the key residues Trp(57) and Tyr(94) at the FMN binding site. Neutral semiquinone radicals of the...

journal_title：Biophysical journal

authors： Medina M,Lostao A,Sancho J,Gómez-Moreno C,Cammack R,Alonso PJ,Martínez JI

更新日期：1999-09-01 00:00:00

abstract：:The interaction of an amphiphilic, 40-amino acid beta-amyloid (Abeta) peptide with liposomal membranes as a function of sterol mole fraction (X(sterol)) was studied based on the fluorescence anisotropy of a site-specific membrane sterol probe, dehydroergosterol (DHE), and fluorescence resonance energy transfer (FRET) ...

journal_title：Biophysical journal

authors： Qiu L,Lewis A,Como J,Vaughn MW,Huang J,Somerharju P,Virtanen J,Cheng KH

更新日期：2009-05-20 00:00:00

abstract：:Many organs adapt to their mechanical environment as a result of physiological change or disease. Cells are both the detectors and effectors of this process. Though many studies have been performed in vitro to investigate the mechanisms of detection and adaptation to mechanical strains, the cellular strains remain unk...

journal_title：Biophysical journal

authors： Charras GT,Horton MA

更新日期：2002-08-01 00:00:00

abstract：:Gaining information about receptor profiles on cells, and subsequently finding the most efficient ligands for these signaling receptors, remain challenging tasks in stem cell and cancer research as well as drug development. We introduce a live-cell method with great potential in both screening for surface receptors an...

journal_title：Biophysical journal

authors： Wienken U,Gaub HE

更新日期：2013-12-17 00:00:00

abstract：:Sedimentation velocity analytical ultracentrifugation is an important tool in the characterization of macromolecules and nanoparticles in solution. The sedimentation coefficient distribution c(s) of Lamm equation solutions is based on the approximation of a single, weight-average frictional coefficient of all particle...

journal_title：Biophysical journal

authors： Brown PH,Schuck P

更新日期：2006-06-15 00:00:00

abstract：:Association of transmembrane (TM) helices is facilitated by the close packing of small residues present along the amino-acid sequence. Extensive studies have established the role of such small residue motifs (GxxxG) in the dimerization of Glycophorin A (GpA) and helped to elucidate the association of TM domains in the...

journal_title：Biophysical journal

authors： Prakash A,Janosi L,Doxastakis M

更新日期：2010-12-01 00:00:00

abstract：:To investigate the mechanisms by which vesicles of pulmonary surfactant adsorb to an air-liquid interface, we measured the effect of different phospholipids and of their concentration both in the subphase and at the interface on this process. Adsorbing vesicles contained the hydrophobic surfactant proteins mixed with ...

journal_title：Biophysical journal

authors： Walters RW,Jenq RR,Hall SB

更新日期：2000-01-01 00:00:00

abstract：:Two isoforms of the sarcoplasmic reticulum Ca2+ release channel (ryanodine receptor or RYR) are expressed together in the skeletal muscles of most vertebrates. We have studied physiological properties of the two isoforms (alpha and beta) by comparing SR preparations from specialized fish muscles that express the alpha...

journal_title：Biophysical journal

authors： O'Brien J,Valdivia HH,Block BA

更新日期：1995-02-01 00:00:00

abstract：:The Mie theory of scattering is used to provide new information on how changes in particle volume, with no change in dry weight, should influence light scattering for various scattering angles and particle sizes. Many biological cells (e.g., algal cells, erythrocytes) and large subcellular structures (e.g., chloroplas...

journal_title：Biophysical journal

authors： Latimer P,Pyle BE

更新日期：1972-07-01 00:00:00