Abstract：
:The biological influence of radiation on living matter has been studied for years; however, several questions about the detailed mechanism of radiation damage formation remain largely unanswered. Among all biomolecules exposed to radiation, DNA plays an important role because any damage to its molecular structure can affect the whole cell and may lead to chromosomal rearrangements resulting in genomic instability or cell death. To identify and characterize damage induced in the DNA sugar-phosphate backbone, in this work we performed x-ray absorption spectroscopy at the P K-edge on DNA irradiated with either UVA light or protons. By combining the experimental results with theoretical calculations, we were able to establish the types and relative ratio of lesions produced by both UVA and protons around the phosphorus atoms in DNA.
journal_name
Biophys Jjournal_title
Biophysical journalauthors
Czapla-Masztafiak J,Szlachetko J,Milne CJ,Lipiec E,Sá J,Penfold TJ,Huthwelker T,Borca C,Abela R,Kwiatek WMdoi
10.1016/j.bpj.2016.01.031subject
Has Abstractpub_date
2016-03-29 00:00:00pages
1304-11issue
6eissn
0006-3495issn
1542-0086pii
S0006-3495(16)00141-7journal_volume
110pub_type
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