Low-resolution molecular structures of isolated functional units from arthropodan and molluscan hemocyanin.

abstract：:Helicobacter pylori arginase, a bimetallic enzyme, is crucial for pathogenesis of the bacterium in human stomach. Despite conservation of the signature motifs in all arginases, the H. pylori homolog has a non-conserved motif (153ESEEKAWQKLCSL165), whose role was recently shown to be critical for its stability and func...

journal_title：Biophysical journal

authors： George G,Kombrabail M,Raninga N,Sau AK

更新日期：2017-03-28 00:00:00

abstract：:Understanding cellular membrane processes is critical for the study of events such as viral entry, neurotransmitter exocytosis, and immune activation. Supported lipid bilayers are commonly used to model these membrane processes experimentally. Despite the relative simplicity of such a system, many important structural...

journal_title：Biophysical journal

authors： Kasson PM,Pande VS

更新日期：2004-06-01 00:00:00

abstract：:The origin of Ibetanull, the Ca2+ current of myotubes from mice lacking the skeletal dihydropyridine receptor (DHPR) beta1a subunit, was investigated. The density of Ibetanull was similar to that of Idys, the Ca2+ current of myotubes from dysgenic mice lacking the skeletal DHPR alpha1S subunit (-0.6 +/- 0.1 and -0.7 +...

journal_title：Biophysical journal

authors： Strube C,Beurg M,Sukhareva M,Ahern CA,Powell JA,Powers PA,Gregg RG,Coronado R

更新日期：1998-07-01 00:00:00

abstract：:Cytochrome c3 (cyt c3) can mediate electron transport across phosphatidylcholine (PC)/cardiolipin (CL) and PC/phosphatidylglycerol (PG) membranes. A two-molecule process is involved in the electron transport across PC/CL membranes in the liquid-crystalline state. In contrast, a single-molecule process dominates the el...

journal_title：Biophysical journal

authors： Kim SM,Yamamoto T,Todokoro Y,Takayama Y,Fujiwara T,Park JS,Akutsu H

更新日期：2006-01-15 00:00:00

abstract：:The stability of the structure of double-stranded DNA in the salt-free solution is discussed on the basis of the polyelectrolyte theory. Assuming that DNA is an infinitely long rod, and the formation of double strands is divided into combining process and folding process, the free energy changes required in these proc...

journal_title：Biophysical journal

authors： OHNISHI T

更新日期：1963-11-01 00:00:00

abstract：:Moesin, a protein of the ezrin, radixin, and moesin family, which links the plasma membrane to the cytoskeleton, is involved in multiple physiological and pathological processes, including viral budding and infection. Its interaction with the plasma membrane occurs via a key phosphoinositide, the phosphatidyl(4,5)inos...

journal_title：Biophysical journal

authors： Lubart Q,Vitet H,Dalonneau F,Le Roy A,Kowalski M,Lourdin M,Ebel C,Weidenhaupt M,Picart C

更新日期：2018-01-09 00:00:00

abstract：:The vibrational spectroscopy and population dynamics of excited singlet (2(1)Ag), excited triplet (3B u), and the ground (1Ag) electronic states of carotenoids in chromatophores of Chromatium vinosum (mainly spirilloxanthin and rhodopin) and of the same carotenoids in benzene solutions are examined by picosecond time-...

journal_title：Biophysical journal

authors： Hayashi H,Noguchi T,Tasumi M,Atkinson GH

更新日期：1991-07-01 00:00:00

abstract：:Bistability is a major mechanism for cellular decision making and usually results from positive feedback in biochemical control systems. Here we show theoretically that bistability between unbound and bound states of adhesion clusters results from positive feedback mediated by structural rather than biochemical proces...

journal_title：Biophysical journal

authors： Erdmann T,Schwarz US

更新日期：2006-09-15 00:00:00

abstract：:We study the lateral headgroup interactions among phosphatidylcholine (PC) molecules and among phosphatidylethanolamine (PE) molecules in monolayers and extend our previous models. In this paper, we present an extensive set of pressure-area isotherms and surface potential experiments on monolayers of phospholipids ran...

journal_title：Biophysical journal

authors： Mingins J,Stigter D,Dill KA

更新日期：1992-06-01 00:00:00

abstract：:The chemical shifts of specific (13)C and (15)N labels distributed throughout KIAGKIA-KIAGKIA-KIAGKIA (K3), an amphiphilic 21-residue antimicrobial peptide, prove that the peptide is in an all alpha-helical conformation in the bilayers of multilamellar vesicles (MLVs) containing dipalmitoylphosphatidylcholine and dipa...

journal_title：Biophysical journal

authors： Toke O,Maloy WL,Kim SJ,Blazyk J,Schaefer J

更新日期：2004-07-01 00:00:00

abstract：:Molecular dynamics simulations were performed to describe the function of the ion-channel-forming toxin α-hemolysin (αHL) in lipid membranes that were composed of either 1,2-diphytanoyl-sn-glycero-3-phospho-choline or 1-palmitoyl-2-oleoyl-sn-glycero-3-phospho-choline. The simulations highlight the importance of lipid ...

journal_title：Biophysical journal

authors： Guros NB,Balijepalli A,Klauda JB

更新日期：2018-11-06 00:00:00

abstract：:The average sizes of fluid and gel domains in the two-component, two-phase system formed from mixtures of dimyristoyl phosphatidylcholine and distearoyl phosphatidylcholine were determined from an analysis of the electron spin resonance spectral lineshapes of a dimyristoyl phosphatidylcholine-nitroxide spin label as a...

journal_title：Biophysical journal

authors： Sankaram MB,Marsh D,Thompson TE

更新日期：1992-08-01 00:00:00

abstract：:To determine if hydrophobic surfactant proteins affect the stability of pulmonary surfactant monolayers at an air/water interface, the studies reported here compared the kinetics of collapse for the complete set of lipids in calf surfactant with and without the proteins. Monomolecular films spread at the surface of ca...

journal_title：Biophysical journal

authors： Lhert F,Yan W,Biswas SC,Hall SB

更新日期：2007-12-15 00:00:00

abstract：:The crystal structure of the Mycobacterium tuberculosis homolog of the bacterial mechanosensitive channel of large conductance (Tb-MscL) provides a unique opportunity to consider mechanosensitive signal transduction at the atomic level. Molecular dynamics simulations of the Tb-MscL channel embedded in an explicit lipi...

journal_title：Biophysical journal

authors： Elmore DE,Dougherty DA

更新日期：2001-09-01 00:00:00

abstract：:It is widely assumed that the decay of fluorescence in photosynthetic systems can be described as a sum of exponential components and that the amplitude of each component is directly related to the absorption cross-section of the antenna pigments coupled to the fluorescing species. We present exact calculations of exc...

journal_title：Biophysical journal

authors： Jean JM,Chan CK,Fleming GR,Owens TG

更新日期：1989-12-01 00:00:00

abstract：:When cells migrate through constricting pores, they incur DNA damage and develop genomic variation. Experiments show that this damage is not due to DNA breakage from mechanical stress on chromatin in the deformed nucleus. Here we propose a model for a mechanism by which nuclear deformation can lead to DNA damage. We t...

journal_title：Biophysical journal

authors： Bennett RR,Pfeifer CR,Irianto J,Xia Y,Discher DE,Liu AJ

更新日期：2017-06-06 00:00:00

abstract：:The development of strategies to measure plasma membrane osmotic water permeability (Pf) in epithelial cells has been motivated by the identification of a family of molecular water channels. A general approach utilizing interferometry to measure cell shape and volume was developed and applied to measure Pf in cell lay...

journal_title：Biophysical journal

authors： Farinas J,Verkman AS

更新日期：1996-12-01 00:00:00

abstract：:Functional RNAs can fold into intricate structures using a number of different secondary and tertiary structural motifs. Many factors contribute to the overall free energy of the target fold. This study aims at quantifying the entropic costs coming from the loss of conformational freedom when the sugar-phosphate backb...

journal_title：Biophysical journal

authors： Mak CH,Phan ENH

更新日期：2018-05-08 00:00:00

abstract：:The pore-forming alpha 1 subunit of L-type calcium (Ca2+) channels is the molecular target of Ca2+ channel blockers such as phenylalkylamines (PAAs). Association and dissociation rates of (-)devapamil were compared for a highly PAA-sensitive L-type Ca2+ channel chimera (Lh) and various class A Ca2+ channel mutants. Th...

journal_title：Biophysical journal

authors： Degtiar VE,Aczél S,Döring F,Timin EN,Berjukow S,Kimball D,Mitterdorfer J,Hering S

更新日期：1997-07-01 00:00:00

abstract：:Formation of liquid-ordered domains in model membranes can be linked to raft formation in cellular membranes. The lipid stoichiometry has a governing influence on domain formation and consequently, biochemical hydrolysis of specific lipids has the potential to remodel domain features. Activation of phospholipase A(2) ...

journal_title：Biophysical journal

authors： Simonsen AC

更新日期：2008-05-15 00:00:00

abstract：:Halorhodopsin (HR), the light-driven chloride transport pigment of Halobacterium halobium, was bleached and reconstituted with retinal analogues with the pi electron system interrupted at different locations (dihydroretinals). The absorption maxima of the artificial pigments formed with the dihydroretinals are found t...

journal_title：Biophysical journal

authors： Lanyi JK,Zimányi L,Nakanishi K,Derguini F,Okabe M,Honig B

更新日期：1988-02-01 00:00:00

abstract：:Homooligomerization of proline utilization A (PutA) bifunctional flavoenzymes is intimately tied to catalytic function and substrate channeling. PutA from Bradyrhizobium japonicum (BjPutA) is unique among PutAs in that it forms a tetramer in solution. Curiously, a dimeric BjPutA hot spot mutant was previously shown to...

journal_title：Biophysical journal

authors： Korasick DA,Campbell AC,Christgen SL,Chakravarthy S,White TA,Becker DF,Tanner JJ

更新日期：2018-06-19 00:00:00

abstract：:Light adaptation in cones was characterized by measuring the changes in temporal frequency responses to sinusoidal modulation of light around various mean levels spanning a range of four log units. We have shown previously that some aspects of cone adaptation behavior can be accounted for by a biochemical kinetic mode...

journal_title：Biophysical journal

authors： Tranchina D,Sneyd J,Cadenas ID

更新日期：1991-07-01 00:00:00

abstract：:Dim-light vision is mediated by retinal rod cells. Rhodopsin (R), a G-protein-coupled receptor, switches to its active form (R(∗)) in response to absorbing a single photon and activates multiple copies of the G-protein transducin (G) that trigger further downstream reactions of the phototransduction cascade. The class...

journal_title：Biophysical journal

authors： Schöneberg J,Heck M,Hofmann KP,Noé F

更新日期：2014-09-02 00:00:00

abstract：:Odorant-induced currents in mammalian olfactory receptor neurons have proved difficult to obtain reliably using conventional whole-cell recording. By using a mathematical model of the electrical circuit of the patch and rest-of-cell, we demonstrate how cell-attached patch measurements can be used to quantitatively ana...

journal_title：Biophysical journal

authors： Chiu P,Lynch JW,Barry PH

更新日期：1997-03-01 00:00:00

abstract：:The yolk granule is the most abundant membrane-bound organelle present in sea urchin eggs and embryos. The major protein component of this organelle, toposome, accounts for approximately 50% of the total yolk protein and has been shown to be localized to the embryonic cell surface. Extensive characterization in severa...

journal_title：Biophysical journal

authors： Hayley M,Emberley J,Davis PJ,Morrow MR,Robinson JJ

更新日期：2006-12-15 00:00:00

abstract：:Stationary and nonstationary correlation-frequency analysis of heterodyne laser light scattering were utilized to make automated, on-line, objective measurements of tracheal ciliary beat frequency (CBF) in intact, anesthetized canines. The stationary correlation-frequency analysis laser light-scattering technique was ...

journal_title：Biophysical journal

authors： Chandra T,Yeates DB,Miller IF,Wong LB

更新日期：1994-03-01 00:00:00

abstract：:Carbonic anhydrase (CA) is a thoroughly studied enzyme. Its primary role is the rapid interconversion of carbon dioxide and bicarbonate in the cells, where carbon dioxide is produced, and in the lungs, where it is released from the blood. At the same time, it regulates pH homeostasis. The inhibitory function of sulfon...

journal_title：Biophysical journal

authors： Jonsson BH,Liljas A

更新日期：2020-10-06 00:00:00

abstract：:The use of propagation invariant Bessel beams has enabled high-resolution subcellular light sheet fluorescence microscopy. However, the energy within the concentric side lobe structure of Bessel beams increases significantly with propagation length, generating unwanted out-of-focus fluorescence that enforces practical...

journal_title：Biophysical journal

authors： Dean KM,Roudot P,Welf ES,Danuser G,Fiolka R

更新日期：2015-06-16 00:00:00

abstract：:One theory of freezing damage suggests that slowly cooled cells are killed by being exposed to increasing concentrations of electrolytes as the suspending medium freezes. A corollary to this view is that protective additives such as glycerol protect cells by acting colligatively to reduce the electrolyte concentration...

journal_title：Biophysical journal

authors： Rall WF,Mazur P,Souzu H

更新日期：1978-07-01 00:00:00