Model for the light-harvesting complex I (B875) of Rhodobacter sphaeroides.

abstract：:The TRPV1 cation nonselective ion channel plays an essential role in thermosensation and perception of other noxious stimuli. TRPV1 can be activated by low extracellular pH, high temperature, or naturally occurring pungent molecules such as allicin, capsaicin, or resiniferatoxin. Its noxious thermal sensitivity makes ...

journal_title：Biophysical journal

authors： Ladrón-de-Guevara E,Dominguez L,Rangel-Yescas GE,Fernández-Velasco DA,Torres-Larios A,Rosenbaum T,Islas LD

更新日期：2020-02-25 00:00:00

abstract：:The 77-K resonance Raman vibrational spectrum of intact goldfish rod photoreceptors containing 3,4-dehydro (A2) retinal is dominated by scattering from the 9-cis component of the steady state at all excitation wavelengths. Intact goldfish photoreceptors were regenerated with an A1-retinal chromophore to determine whet...

journal_title：Biophysical journal

authors： Barry B,Mathies RA,Pardoen JA,Lugtenburg J

更新日期：1987-10-01 00:00:00

abstract：:To characterize elastic properties and geometrical parameters of individual, whole myosin molecules during their interaction with actin we sparsely adsorbed myosin molecules to nanometer-sized microspheres. Thermally driven position fluctuations of these microspheres were recorded with the three-dimensional detection ...

journal_title：Biophysical journal

authors： Scholz T,Altmann SM,Antognozzi M,Tischer C,Hörber JK,Brenner B

更新日期：2005-01-01 00:00:00

abstract：:Diverse microbial communities coordinate group behaviors through signal exchange, such as the exchange of acyl-homoserine lactones (AHLs) by Gram-negative bacteria. Cellular communication is prone to interference by neighboring microbes. One mechanism of interference is signal destruction through the production of an ...

journal_title：Biophysical journal

authors： Silva KP,Chellamuthu P,Boedicker JQ

更新日期：2017-03-14 00:00:00

abstract：:A simple technique for forming "black" lipid bilayer membranes containing negligible amounts of alkyl solvent is described. The membranes are formed by the method of Mueller et al (Circulation. 1962. 26:1167.) from glyceryl monooleate (GMO) dispersed in squalene. The squalene forms an annulus to satisfy the boundary c...

journal_title：Biophysical journal

authors： White SH

更新日期：1978-09-01 00:00:00

abstract：:The effects of Hofmeister anions on the perpendicular and lateral equation-of-state (EOS) of the dipalmitoylphosphatidylcholine lamellar phase discussed in the companion article are here examined using appropriate free energy models for the intra- and interbilayer interactions. Minimizing the free energy with respect ...

journal_title：Biophysical journal

authors： Leontidis E,Aroti A,Belloni L,Dubois M,Zemb T

更新日期：2007-09-01 00:00:00

abstract：:A long-standing question about membrane structure and function is the degree to which the physical properties of the inner and outer leaflets of a bilayer are coupled to one another. Using our recently developed methods to prepare asymmetric vesicles, coupling was investigated for vesicles containing phosphatidylcholi...

journal_title：Biophysical journal

authors： Chiantia S,London E

更新日期：2012-12-05 00:00:00

abstract：:Sarcoplasmic reticulum (SR) Ca2+ ATPase was derivatized with 4,4'-diisothiocyanatostilbene-2,2'-sulfonic acid (DIDS), and complete enzyme inactivation was produced with a molecular stoichiometry of one DIDS per ATPase. It was determined by peptide analysis and sequencing that Lys492 and Lys515 were the ATPase residues...

journal_title：Biophysical journal

authors： Hua S,Inesi G

更新日期：1997-10-01 00:00:00

abstract：:Purple membranes were adsorbed on freestanding lipid bilayers, termed nano-black lipid membranes (nano-BLMs), suspending the pores of porous alumina substrates with average pore diameters of 280 nm. Nano-BLMs were obtained by first coating the upper surface of the highly ordered porous alumina substrates with a thin g...

journal_title：Biophysical journal

authors： Horn C,Steinem C

更新日期：2005-08-01 00:00:00

abstract：:A pulsed source neutron spectrometer has been used to measure vibrational spectra (20-4000 cm-1) of dry and hydrated type I collagen fibers, and of two model polypeptides, polyproline II and (prolyl-prolyl-glycine)10, at temperatures of 30 and 120 K. the collagen spectra provide the first high resolution neutron views...

journal_title：Biophysical journal

authors： Middendorf HD,Hayward RL,Parker SF,Bradshaw J,Miller A

更新日期：1995-08-01 00:00:00

abstract：:Monolayers prepared from myelin conserve all the compositional complexity of the natural membrane when spread at the air-water interface. They show a complex pressure-dependent surface pattern that, on compression, changes from the coexistence of two liquid phases to a viscous fractal phase embedded in a liquid phase....

journal_title：Biophysical journal

authors： Rosetti CM,Maggio B

更新日期：2007-12-15 00:00:00

abstract：:We have measured the rates of insertion into, desorption from, and spontaneous interlayer translocation (flip-flop) in liquid-disordered and liquid-ordered phase lipid bilayer membranes, of the fluorescent phospholipid derivative NBD-dimyristoylphosphatidyl ethanolamine. This study made use of a recently described met...

journal_title：Biophysical journal

authors： Abreu MS,Moreno MJ,Vaz WL

更新日期：2004-07-01 00:00:00

abstract：:Immobilization is a key step involved in probing molecular interactions using single-molecule force spectroscopy methods, including atomic force microscopy (AFM). To our knowledge, we describe a novel approach termed flexible nanoarray (FNA) in which the interaction between the two internally immobilized amyloid β pep...

journal_title：Biophysical journal

authors： Krasnoslobodtsev AV,Zhang Y,Viazovkina E,Gall A,Bertagni C,Lyubchenko YL

更新日期：2015-05-05 00:00:00

abstract：:We compared wild-type rat skeletal muscle NaChs (micro1) and a mutant NaCh (Y1586K) that has a single amino acid substitution, lysine (K) for tyrosine (Y), at position 1586 in the S6 transmembrane segment of domain 4. In Y1586K, macroscopic current decay is faster, the V(1/2) of the activation curve is shifted in the ...

journal_title：Biophysical journal

authors： O'Reilly JP,Wang SY,Wang GK

更新日期：2000-02-01 00:00:00

abstract：:Cholesterol is an essential molecule in the membranes of mammalian cells. It is known to be distributed heterogeneously within the cells, between the bilayer leaflets, as well as between lateral domains within the bilayer. However, we do not know exactly how cholesterol is distributed and what forces drive this sortin...

journal_title：Biophysical journal

authors： Nyholm TKM,Jaikishan S,Engberg O,Hautala V,Slotte JP

更新日期：2019-01-22 00:00:00

abstract：:The space-clamped squid axon membrane and two versions of the Hodgkin-Huxley model (the original, and a strongly adapting version) are subjected to a first order dynamic analysis. Stable, repetitive firing is induced by phase-locking nerve impulses to sinusoidal currents. The entrained impulses are then pulse position...

journal_title：Biophysical journal

authors： Fohlmeister JF,Adelman WJ Jr,Poppele RE

更新日期：1980-04-01 00:00:00

abstract：:We are interested in modeling the membrane-spanning domain of the serotonin 5-HT1A G-protein coupled receptor. This superfamily of proteins is predicted to share the topology of the seven transmembrane helices of bacteriorhodopsin (BR), even though no significant sequence homology had been identified. We found signifi...

journal_title：Biophysical journal

authors： Ballesteros JA,Weinstein H

更新日期：1992-04-01 00:00:00

abstract：:The intrinsic birefringences of TMV, tropocollagen, and paramyosin were calculated from flow birefringence measurements using the theory of Peterlin and Stuart. The values are -0.029, -0.029, and -0.030, respectively. The intrinsic birefringences of TMV and tropocollagen were measured as a function of the refractive i...

journal_title：Biophysical journal

authors： TAYLOR EW,CRAMER W

更新日期：1963-03-01 00:00:00

abstract：:Critical to biological processes such as secretion and transport, protein-lipid interactions within the membrane and at the membrane-water interface still raise many questions. Here we examine the role of lipid headgroups in these interactions by using gramicidin A (gA) channels in planar bilayers as a probe. We show ...

journal_title：Biophysical journal

authors： Rostovtseva TK,Petrache HI,Kazemi N,Hassanzadeh E,Bezrukov SM

更新日期：2008-02-15 00:00:00

abstract：:The channel-forming properties of two analogs of gramicidin, gramicidin-ethylenediamine (gram-EDA), and gramicidin-N,N-dimethylethylenediamine (gram-DMEDA) were studied in planar lipid bilayers, using protons as the permeant ion. These peptides have positively charged amino groups tethered to their C-terminal ends via...

journal_title：Biophysical journal

authors： Woolley GA,Zunic V,Karanicolas J,Jaikaran AS,Starostin AV

更新日期：1997-11-01 00:00:00

abstract：:Historically, predicting macroscopic blood flow characteristics such as viscosity has been an empirical process due to the difficulty in rigorously including the particulate nature of blood in a mathematical representation of blood rheology. Using a two-dimensional lattice Boltzmann approach, we have simulated the flo...

journal_title：Biophysical journal

authors： Sun C,Munn LL

更新日期：2005-03-01 00:00:00

abstract：:An equation is derived, which connects two functions P(r) and g(x), the first being related to the scattering intensity by a simple transformation, the second to the elctron density distribution of a spherically symmetric structure. This relation seems to be a suitable starting point for an analysis of shell structure...

journal_title：Biophysical journal

authors： Weick D

更新日期：1974-03-01 00:00:00

abstract：:The solution structures of the N-terminal domains of protein S, a plasma vitamin K-dependent glycoprotein, and its homolog growth arrest specific protein 6 (Gas6) were predicted by molecular dynamics computer simulations. The initial structures were based on the x-ray crystallographic structure of the corresponding re...

journal_title：Biophysical journal

authors： Perera L,Li L,Darden T,Monroe DM,Pedersen LG

更新日期：1997-10-01 00:00:00

abstract：:We develop a model of tyrosine phosphorylation and activation of the T-cell receptor (TCR) by localization to regions of close membrane-membrane proximity (close contact) that physically exclude tyrosine phosphatases such as CD45. Phosphatase exclusion generates regions of low phosphatase and high kinase activity and ...

journal_title：Biophysical journal

authors： Burroughs NJ,Lazic Z,van der Merwe PA

更新日期：2006-09-01 00:00:00

abstract：:Biological spread cells exist in a perpetually fluctuating state and therefore cannot be described in terms of a unique deterministic system. For modeling approaches to provide novel insight and uncover new mechanisms that drive cell behavior, a framework is required that progresses from traditional deterministic meth...

journal_title：Biophysical journal

authors： McEvoy E,Shishvan SS,Deshpande VS,McGarry JP

更新日期：2018-12-18 00:00:00

abstract：:When a light beam is focused on a muscle preparation which is allowed to contract, large changes in the intensity of the emerging light accompany the contraction. These movement-related optical signals were studied and compared to simultaneous measurements of force in isolated cardiac Purkinje fibers. The two signals ...

journal_title：Biophysical journal

authors： Kass RS

更新日期：1981-04-01 00:00:00

abstract：:Two isoforms of the sarcoplasmic reticulum Ca2+ release channel (ryanodine receptor or RYR) are expressed together in the skeletal muscles of most vertebrates. We have studied physiological properties of the two isoforms (alpha and beta) by comparing SR preparations from specialized fish muscles that express the alpha...

journal_title：Biophysical journal

authors： O'Brien J,Valdivia HH,Block BA

更新日期：1995-02-01 00:00:00

abstract：:P188, a triblock copolymer of the form poly(ethylene oxide)-poly(propylene oxide)-poly(ethylene oxide) helps seal electroporated cell membranes, arresting the leakage of intracellular materials from the damaged cells. To explore the nature of the interaction between P188 and cell membranes, we have constructed a model...

journal_title：Biophysical journal

authors： Maskarinec SA,Hannig J,Lee RC,Lee KY

更新日期：2002-03-01 00:00:00

abstract：:Synthetic filaments prepared from column-purified rabbit skeletal myosin by slow dialysis exhibit characteristic bipolar organization and 14-nm axial subunit spacing. Backbone substructure can be discerned in high resolution micrographs in the form of striations of 3--4-nm width and slight angular tilt from the direct...

journal_title：Biophysical journal

authors： Koretz JF

更新日期：1979-09-01 00:00:00

abstract：:CLC anion channels are homodimeric proteins. Each subunit is comprised of 18 α-helices designated "A-R" and an intracellular carboxy-terminus containing two cystathionine-β-synthase (CBS1 and CBS2) domains. Conformational coupling between membrane and intracellular domains via poorly understood mechanisms is required ...

journal_title：Biophysical journal

authors： Yamada T,Strange K

更新日期：2016-11-01 00:00:00