Interfacial polar interactions affect gramicidin channel kinetics.

abstract：:Nipah is an emerging paramyxovirus that is of serious concern to human health. It invades host cells using two of its membrane proteins-G and F. G binds to host ephrins and this stimulates G to activate F. Upon activation, F mediates virus-host membrane fusion. Here we focus on mechanisms that underlie the stimulation...

journal_title：Biophysical journal

authors： Dutta P,Siddiqui A,Botlani M,Varma S

更新日期：2016-10-18 00:00:00

abstract：:The solution-scattering profiles of macromolecules are significantly affected by the thermal motions of their atoms, especially at wide scattering angles, even when only a single conformational state is significantly populated in solution. Here it is shown that the impact thermal motions have on the molecular componen...

journal_title：Biophysical journal

authors： Moore PB

更新日期：2014-04-01 00:00:00

abstract：:KATP channels assemble from four regulatory SUR1 and four pore-forming Kir6.2 subunits. At the single-channel current level, ATP-dependent gating transitions between the active burst and the inactive interburst conformations underlie inhibition of the KATP channel by intracellular ATP. Previously, we identified a slow...

journal_title：Biophysical journal

authors： Drain P,Geng X,Li L

更新日期：2004-04-01 00:00:00

abstract：:We study a Brownian dynamics simulation model of a biopolymeric shell deformed by axial forces exerted at opposing poles. The model exhibits two distinct, linear force-extension regimes, with the response to small tensions governed by linear elasticity and the response to large tensions governed by an effective spring...

journal_title：Biophysical journal

authors： Banigan EJ,Stephens AD,Marko JF

更新日期：2017-10-17 00:00:00

abstract：:How calmodulin (CaM) acts in KRAS-driven cancers is a vastly important question. CaM binds to and stimulates PI3Kα/Akt signaling, promoting cell growth and proliferation. Phosphorylation of CaM at Tyr99 (pY99) enhances PI3Kα activation. PI3Kα is a lipid kinase. It phosphorylates PIP2 to produce PIP3, to which Akt bind...

journal_title：Biophysical journal

authors： Zhang M,Jang H,Gaponenko V,Nussinov R

更新日期：2017-11-07 00:00:00

abstract：:We developed a multicellular model characterized by a high degree of heterogeneity to investigate possible mechanisms that underlie circadian network synchronization and rhythmicity in the suprachiasmatic nucleus (SCN). We populated a two-dimensional grid with 400 model neurons coupled via γ-aminobutyric acid (GABA) a...

journal_title：Biophysical journal

authors： Vasalou C,Herzog ED,Henson MA

更新日期：2011-07-06 00:00:00

abstract：:BBL is an independent folding domain of a large multienzyme complex, 2-oxoglutarate dehydrogenase. The folding mechanism of BBL is under debate between the views of noncooperative downhill-type and classical two-state. Extensive replica exchange molecular dynamics simulations of BBL in explicit solvent have shown some...

journal_title：Biophysical journal

authors： Fan J,Duan M,Li DW,Wu H,Yang H,Han L,Huo S

更新日期：2011-05-18 00:00:00

abstract：:Synchrotron x-ray diffraction, differential scanning calorimetry, and electron spin resonance spectroscopy have been employed to characterize a quasicrystalline phase formed in aqueous dispersions of binary mixtures of glucocerebroside and palmitoyloleoylphosphatidylethanolamine. Small- and wide-angle x-ray scattering...

journal_title：Biophysical journal

authors： Feng Y,Rainteau D,Chachaty C,Yu ZW,Wolf C,Quinn PJ

更新日期：2004-04-01 00:00:00

abstract：:Impact loading of articular cartilage causes extensive chondrocyte death. Cell membranes have a limited elastic range of 3-4% strain but are protected from direct stretch during physiological loading by their membrane reservoir, an intricate pattern of membrane folds. Using a finite-element model, we suggested previou...

journal_title：Biophysical journal

authors： Moo EK,Amrein M,Epstein M,Duvall M,Abu Osman NA,Pingguan-Murphy B,Herzog W

更新日期：2013-10-01 00:00:00

abstract：:We report on the application of two photon molecular excitation to fluorescence correlation spectroscopy. We demonstrate the first fluorescence correlation spectroscopy measurements of translational mobility in the cytoplasm of living cells. Two-photon excitation inherently excites small sample volumes in three dimens...

journal_title：Biophysical journal

authors： Berland KM,So PT,Gratton E

更新日期：1995-02-01 00:00:00

abstract：:Theories of DNA electrophoretic separations generally treat the DNA as a free draining polymer moving in an electric field at a rate that depends on the effective charge density of the molecule. Separations can occur in sieving media ranging from ultradilute polymer solutions to tightly cross-linked gels. It has recen...

journal_title：Biophysical journal

authors： Ferree S,Blanch HW

更新日期：2004-07-01 00:00:00

abstract：:Maintenance of paroxysmal atrial fibrillation (AF) by fast rotors in the left atrium (LA) or at the pulmonary veins (PVs) is not fully understood. To gain insight into this dynamic and complex process, we studied the role of the heterogeneous distribution of transmembrane currents in the PVs and LA junction (PV-LAJ) i...

journal_title：Biophysical journal

authors： Calvo CJ,Deo M,Zlochiver S,Millet J,Berenfeld O

更新日期：2014-04-15 00:00:00

abstract：:The effect of uncharged, dipolar phloretin on anion and cation conductance through a black lipid membrane can be used to study its adsorption behavior. The adsorption of phloretin can be described by a Langmuir isotherm with weak dipole-dipole interaction. ...

journal_title：Biophysical journal

authors： de Levie R,Rangarajan SK,Seelig PF,Andersen OS

更新日期：1979-02-01 00:00:00

abstract：:Dimerization of the transmembrane (TM) adaptor protein DAP12 plays a key role in mediating activation signals through TM-TM association with cell-surface receptors. Herein, we apply the TOXCAT assay and molecular dynamics simulation to analyze dynamics and dimerization of the TM helix of DAP12 in the membrane bilayer....

journal_title：Biophysical journal

authors： Wei P,Zheng BK,Guo PR,Kawakami T,Luo SZ

更新日期：2013-04-02 00:00:00

abstract：:We have used admittance analysis together with the black lipid membrane technique to analyze electrogenic reactions within the Na(+) branch of the reaction cycle of the Na(+)/K(+)-ATPase. ATP release by flash photolysis of caged ATP induced changes in the admittance of the compound membrane system that are associated ...

journal_title：Biophysical journal

authors： Babes A,Fendler K

更新日期：2000-11-01 00:00:00

abstract：:Formation of liquid-ordered domains in model membranes can be linked to raft formation in cellular membranes. The lipid stoichiometry has a governing influence on domain formation and consequently, biochemical hydrolysis of specific lipids has the potential to remodel domain features. Activation of phospholipase A(2) ...

journal_title：Biophysical journal

authors： Simonsen AC

更新日期：2008-05-15 00:00:00

abstract：:Crystals have been grown of "sperm whale" myoglobin produced in Escherichia coli from a synthetic gene and the structure has been solved to 1.9 A resolution. Because of a remaining initiator methionine, this protein crystallizes in a different space group from native sperm whale myoglobin. The three-dimensional struct...

journal_title：Biophysical journal

authors： Phillips GN Jr

更新日期：1990-02-01 00:00:00

abstract：:Mathematical modeling of the cardiac action potential has proven to be a powerful tool for illuminating various aspects of cardiac function, including cardiac arrhythmias. However, no currently available detailed action potential model accurately reproduces the dynamics of the cardiac action potential and intracellula...

journal_title：Biophysical journal

authors： Mahajan A,Shiferaw Y,Sato D,Baher A,Olcese R,Xie LH,Yang MJ,Chen PS,Restrepo JG,Karma A,Garfinkel A,Qu Z,Weiss JN

更新日期：2008-01-15 00:00:00

abstract：:Hybrid global optimization methods attempt to combine the beneficial features of two or more algorithms, and can be powerful methods for solving challenging nonconvex optimization problems. In this paper, novel classes of hybrid global optimization methods, termed alternating hybrids, are introduced for application as...

journal_title：Biophysical journal

authors： Klepeis JL,Pieja MJ,Floudas CA

更新日期：2003-02-01 00:00:00

abstract：:Two-dimensional 1H NMR spectroscopy over a range of temperature through thermal unfolding has been applied to the low-spin, ferric cyanide complex of myoglobin from Aplysia limacina to search for intermediates in the unfolding and to characterize the effect of temperature on the magnetic properties and electronic stru...

journal_title：Biophysical journal

authors： Xia Z,Nguyen BD,Brunori M,Cutruzzolà F,La Mar GN

更新日期：2005-12-01 00:00:00

abstract：:The diffusion of Ca as it converges to the external mouth of a Ca channel is examined. Diffusional limitation on Ca ions entering Ca channels during current flow, cause local extracellular Ca depletions. Such extracellular Ca depletions have been reported in cardiac muscle. The cardiac sarcolemma has a large number of...

journal_title：Biophysical journal

authors： Bers DM,Peskoff A

更新日期：1991-03-01 00:00:00

abstract：:In this article, optimization-based frameworks are introduced for elucidating the input-output structure of signaling networks and for pinpointing targeted disruptions leading to the silencing of undesirable outputs in therapeutic interventions. The frameworks are demonstrated on a large-scale reconstruction of a sign...

journal_title：Biophysical journal

authors： Dasika MS,Burgard A,Maranas CD

更新日期：2006-07-01 00:00:00

abstract：:The effects of intracellular magnesium ions and extracellular calcium and magnesium ions on the delayed rectifier potassium ion channel, IK, were investigated from intracellularly perfused squid giant axons. Cao+2 and Mgo+2 both blocked IK in a voltage-independent manner with a KD of approximately 100 and 500 mM, resp...

journal_title：Biophysical journal

authors： Clay JR

更新日期：1995-11-01 00:00:00

abstract：:Deciphering the mechanisms underlying the high sensitivity of cells to mechanical microenvironments is crucial for understanding many physiological and pathological processes, e.g., stem cell differentiation and cancer cell metastasis. Here, a cytoskeletal tensegrity model is proposed to study the reorientation of pol...

journal_title：Biophysical journal

authors： Xu GK,Li B,Feng XQ,Gao H

更新日期：2016-10-04 00:00:00

abstract：:We report here a method for the determination of the pKa of histidine in complex or heterogeneous systems amenable to neither solid-state nor solution NMR spectroscopy. Careful synthesis of a fluorenylmethyloxycarbonyl- and trityl-protected, C2-deuterated histidine produces a vibrational-probe-equipped amino acid that...

journal_title：Biophysical journal

authors： Braun GA,Pogostin BH,Pucetaite M,Londergan CH,Åkerfeldt KS

更新日期：2020-11-03 00:00:00

abstract：:The cytoskeletal activity of motile or adherent cells is frequently seen to induce detectable displacements of sufficiently compliant substrata. The physics of this phenomenon is discussed in terms of the classical theory of small-strain, plane-stress elasticity. The main results of such analysis is a transform expres...

journal_title：Biophysical journal

authors： Dembo M,Oliver T,Ishihara A,Jacobson K

更新日期：1996-04-01 00:00:00

abstract：:A new stable substrate applicable to the observation of DNA molecules by atomic force microscopy (AFM) was fabricated from a ultrasmooth sapphire (alpha-Al2O3 single crystal) plate. The atomically ultrasmooth sapphire as obtained by high-temperature annealing has hydrophobic surfaces and could not be used for the AFM ...

journal_title：Biophysical journal

authors： Yoshida K,Yoshimoto M,Sasaki K,Ohnishi T,Ushiki T,Hitomi J,Yamamoto S,Sigeno M

更新日期：1998-04-01 00:00:00

abstract：:The enzyme F1-adenosine triphosphatase (ATPase) is a molecular motor that converts the chemical energy stored in the molecule adenosine triphosphate (ATP) into mechanical rotation of its gamma-subunit. During steady-state catalysis, the three catalytic sites of F1 operate in a cooperative fashion such that at every in...

journal_title：Biophysical journal

authors： Dittrich M,Hayashi S,Schulten K

更新日期：2004-11-01 00:00:00

abstract：:A comparative analysis of two models of anisotropic reactivity in bimolecular diffusion-controlled reaction kinetics is presented. One is the conventional model of reactive patches (MRP), where the surface of a molecule is assumed to be reactive over a certain region (circular patch) with the rest of the surface being...

journal_title：Biophysical journal

authors： Barzykin AV,Shushin AI

更新日期：2001-05-01 00:00:00

abstract：:The effects of myosin regulatory light chain (RLC) phosphorylation and strain on adenosine diphosphate (ADP) release from cross-bridges in phasic (rabbit bladder (Rbl)) and tonic (femoral artery (Rfa)) smooth muscle were determined by monitoring fluorescence transients of the novel ADP analog, 3'-deac-eda-ADP (deac-ed...

journal_title：Biophysical journal

authors： Khromov AS,Webb MR,Ferenczi MA,Trentham DR,Somlyo AP,Somlyo AV

更新日期：2004-04-01 00:00:00