Conformation of a peptide encompassing the proton translocation channel of vacuolar H(+)-ATPase.

abstract：:A mesoscale model of DNA is presented (3SPN.1), extending the scheme previously developed by our group. Each nucleotide is mapped onto three interaction sites. Solvent is accounted for implicitly through a medium-effective dielectric constant and electrostatic interactions are treated at the level of Debye-Hückel theo...

journal_title：Biophysical journal

authors： Sambriski EJ,Schwartz DC,de Pablo JJ

更新日期：2009-03-04 00:00:00

abstract：:We have studied the anion-dependent gating of roflamycoin ion channels using spectral analysis of noise in currents through multichannel planar lipid bilayers. We have found that in addition to low frequency current fluctuations that may be attributed to channel switching between open and closed conformations, roflamy...

journal_title：Biophysical journal

authors： Grigorjev PA,Bezrukov SM

更新日期：1994-12-01 00:00:00

abstract：:Binding of a small molecule to a macromolecular target reduces its conformational freedom, resulting in a negative entropy change that opposes the binding. The goal of this study is to estimate the configurational entropy change of two minor-groove-binding ligands, netropsin and distamycin, upon binding to the DNA dup...

journal_title：Biophysical journal

authors： Dolenc J,Baron R,Oostenbrink C,Koller J,van Gunsteren WF

更新日期：2006-08-15 00:00:00

abstract：:Cyclic 3'5' adenosine monophosphate (cAMP)-dependent-protein kinase (PKA) signaling is a fundamental regulatory pathway for mediating cellular responses to hormonal stimuli. The pathway is activated by high-affinity association of cAMP with the regulatory subunit of PKA and signal termination is achieved upon cAMP dis...

journal_title：Biophysical journal

authors： Krishnamurthy S,Moorthy BS,Xin Xiang L,Xin Shan L,Bharatham K,Tulsian NK,Mihalek I,Anand GS

更新日期：2014-09-16 00:00:00

abstract：:The membrane-lytic peptide melittin has previously been shown to form pores in lipid bilayers that have been described in terms of two different structural models. In the "barrel stave" model the bilayer remains more or less flat, with the peptides penetrating across the bilayer hydrocarbon region and aggregating to f...

journal_title：Biophysical journal

authors： Allende D,Simon SA,McIntosh TJ

更新日期：2005-03-01 00:00:00

abstract：:Estimates of the free myoplasmic [Ca2+] ([Ca2+]i) with fluorescent dyes are complicated by the fact that some properties of these dyes are altered in the intracellular environment. In the present study indo-1 was used to measure [Ca2+]i in isolated muscle fibers from Xenopus frogs. Fluorescent ratio signals obtained f...

journal_title：Biophysical journal

authors： Westerblad H,Allen DG

更新日期：1996-08-01 00:00:00

abstract：:Self-aggregation of isolated plant light-harvesting complexes (LHCs) upon detergent extraction is associated with fluorescence quenching and is used as an in vitro model to study the photophysical processes of nonphotochemical quenching (NPQ). In the NPQ state, in vivo induced under excess solar light conditions, harm...

journal_title：Biophysical journal

authors： Pandit A,Shirzad-Wasei N,Wlodarczyk LM,van Roon H,Boekema EJ,Dekker JP,de Grip WJ

更新日期：2011-11-16 00:00:00

abstract：:Each myosin molecule contains two heavy chains and a total of four low-molecular weight light chain subunits, two "essential" and two "regulatory" light chains (RLCs). Although the roles of myosin light chains in vertebrate striated muscle are poorly understood at present, recent studies on the RLC have suggested that...

journal_title：Biophysical journal

authors： Diffee GM,Greaser ML,Reinach FC,Moss RL

更新日期：1995-04-01 00:00:00

abstract：:Apparent diffusion coefficient (ADC) measurement in the lung using gas magnetic resonance imaging is a promising technique with potential for reflecting changes in lung microstructure. Despite some recent impressive human applications, full interpretation of ADC measures remains an elusive goal, due to a lack of detai...

journal_title：Biophysical journal

authors： Pérez-Sánchez JM,Rodríguez I,Ruiz-Cabello J

更新日期：2009-07-22 00:00:00

abstract：:Here, we report the first direct observation of Van der Waals' attraction between biomembrane capsules using measurements of the free energy reduction per unit area of membrane-membrane contact formation. In these studies, the membrane capsules were reconstituted neutral (egg phosphatidylcholine) lipid bilayers of gia...

journal_title：Biophysical journal

authors： Evans E,Metcalfe M

更新日期：1984-09-01 00:00:00

abstract：:Galactose and other chemotactic attractants have been shown to trigger an apparent hyperpolarization in Escherichia coli (Eisenbach, M., 1982, Biochemistry, 21:6818-6825). The probe used to measure membrane potential in that study, tetraphenylphosphonium (TPP+), may respond also to surface-charge changes in the membra...

journal_title：Biophysical journal

authors： Eisenbach M,Margolin Y,Ciobotariu A,Rottenberg H

更新日期：1984-02-01 00:00:00

abstract：:In this work, we present a protocol to reconstitute membrane proteins into giant unilamellar vesicles (GUV) via peptide-induced fusion. In principle, GUV provide a well-defined lipid matrix, resembling a close-to-native state for biophysical studies, including optical microspectroscopy, of transmembrane proteins at th...

journal_title：Biophysical journal

authors： Kahya N,Pécheur EI,de Boeij WP,Wiersma DA,Hoekstra D

更新日期：2001-09-01 00:00:00

abstract：:A new experimental approach has been developed to study the distribution of local electrostatic potential around specific protons in biologically important molecules. The approach is the development of a method denoted as "spin label/spin probe," which was proposed by one of us (. Mol. Biol. 6:498-507). The proposed m...

journal_title：Biophysical journal

authors： Likhtenshtein GI,Adin I,Novoselsky A,Shames A,Vaisbuch I,Glaser R

更新日期：1999-07-01 00:00:00

abstract：:Protein aggregation is linked to more than 30 human pathologies, including Alzheimer's and Parkinson's diseases. Since small oligomers that form at the beginning of the fibrillation process probably are the most toxic elements, therapeutic strategies involving fibril fragmentation could be detrimental. An alternative ...

journal_title：Biophysical journal

authors： Pagano RS,López Medus M,Gómez GE,Couto PM,Labanda MS,Landolfo L,D'Alessio C,Caramelo JJ

更新日期：2014-08-05 00:00:00

abstract：:A lattice relaxation algorithm is developed to solve the Poisson-Nernst-Planck (PNP) equations for ion transport through arbitrary three-dimensional volumes. Calculations of systems characterized by simple parallel plate and cylindrical pore geometries are presented in order to calibrate the accuracy of the method. A ...

journal_title：Biophysical journal

authors： Kurnikova MG,Coalson RD,Graf P,Nitzan A

更新日期：1999-02-01 00:00:00

abstract：:The impedance measured in a strip of heart tissue from the moth Hyalophora cecropia is fitted by circuit models of several configurations. The circuits include: (a) a single R-C circuit (b) a double R-C circuit (c) terminated transmission lines, and (d) a pattern of cells with cell-to-cell transmission paths. The last...

journal_title：Biophysical journal

authors： Stibitz GR,McCann FV

更新日期：1974-02-01 00:00:00

abstract：:Formation of protrusions and protein segregation on the membrane is of a great importance for the functioning of the living cell. This is most evident in recent experiments that show the effects of the mechanical properties of the surrounding substrate on cell morphology. We propose a mechanism for the formation of me...

journal_title：Biophysical journal

authors： Veksler A,Gov NS

更新日期：2007-12-01 00:00:00

abstract：:Protein solubility in aqueous solutions depends in a complicated and not well understood way on pH, salt type, and salt concentration. Why for instance does the use of two different monovalent salts, potassium thiocyanate and potassium chloride, produce such different results? One important and previously neglected so...

journal_title：Biophysical journal

authors： Boström M,Williams DR,Ninham BW

更新日期：2003-08-01 00:00:00

abstract：:We have measured the kinetics of adsorption of small (12.5-nm radius) unilamellar vesicles onto SiO2, oxidized gold, and a self-assembled monolayer of methyl-terminated thiols, using a quartz crystal microbalance (QCM). Simultaneous measurements of the shift in resonant frequency and the change in energy dissipation a...

journal_title：Biophysical journal

authors： Keller CA,Kasemo B

更新日期：1998-09-01 00:00:00

abstract：:Chinese hamster ovary cells were irradiated with 100-5,000 rads of X-rays and inhibition of the initiation of replicons after irradiation was demonstrated by analyzing nascent DNA sedimented in alkaline sucrose gradients. The renaturation kinetics of DNA synthesized during 60 min of incubation after irradiation was co...

journal_title：Biophysical journal

authors： Mattern MR,Painter RB

更新日期：1977-08-01 00:00:00

abstract：:A newly developed microscope using acousto-optic tunable filters (AOTFs) was used to generate in vivo hemoglobin saturation (SO2) and oxygen tension (PO2) maps in the cerebral cortex of mice. SO2 maps were generated from the spectral analysis of reflected absorbance images collected at different wavelengths, and PO2 m...

journal_title：Biophysical journal

authors： Shonat RD,Wachman ES,Niu W,Koretsky AP,Farkas DL

更新日期：1997-09-01 00:00:00

abstract：:The energy transfer processes between carotenoids and Chls have been studied by femtosecond transient absorption in the CP29-WT complex, which contains only two carotenoids per polypeptide located in the L1 and L2 sites, and in the CP29-E166V mutant in which only the L1 site is occupied. The comparison of these two sa...

journal_title：Biophysical journal

authors： Croce R,Müller MG,Caffarri S,Bassi R,Holzwarth AR

更新日期：2003-04-01 00:00:00

abstract：:We have investigated classical nuclear localization sequence (NLS) mediated protein trafficking by measuring biomolecular dynamics within living cells using two-photon fluorescence correlation spectroscopy. By directly observing the behavior of specific molecules in their native cellular environment, it is possible to...

journal_title：Biophysical journal

authors： Wu J,Corbett AH,Berland KM

更新日期：2009-05-06 00:00:00

abstract：:Nonlinear optical Stokes ellipsometric (NOSE) microscopy was demonstrated for the analysis of collagen-rich biological tissues. NOSE is based on polarization-dependent second harmonic generation imaging. NOSE was used to access the molecular-level distribution of collagen fibril orientation relative to the local fiber...

journal_title：Biophysical journal

authors： Dow XY,DeWalt EL,Sullivan SZ,Schmitt PD,Ulcickas JRW,Simpson GJ

更新日期：2016-10-04 00:00:00

abstract：:The M2 protein from the influenza A virus forms a proton channel in the virion that is essential for infection. This tetrameric protein appears to form a four-helix bundle spanning the viral membrane. Here the solid-state NMR method, 2D polarization inversion spin exchange at magic angle (PISEMA), has been used to obt...

journal_title：Biophysical journal

authors： Song Z,Kovacs FA,Wang J,Denny JK,Shekar SC,Quine JR,Cross TA

更新日期：2000-08-01 00:00:00

abstract：:Cell motion is driven by interplay between the actin cytoskeleton and the cell adhesions in the front part of the cell. The actin network segregates into lamellipodium and lamellum, whereas the adhesion complexes are characteristically distributed underneath the actin system. Here, we suggest a computational model for...

journal_title：Biophysical journal

authors： Shemesh T,Bershadsky AD,Kozlov MM

更新日期：2012-04-18 00:00:00

abstract：:The relation between the dynamic properties of the haptenic site of lipid haptens and the phase transition of the host lattice was investigated using head group spin-labeled phosphatidylethanolamines, that is, spin-label lipid haptens (Brûlet, P., and H. M. McConnell, 1976, Proc. Natl. Acad. Sci. USA., 73:2977-2981; B...

journal_title：Biophysical journal

authors： Takeshita K,Utsumi H,Hamada A

更新日期：1987-08-01 00:00:00

abstract：:We investigate the conditions under which nonideality in solution influences the Donnan equilibrium. Of the various parameters that characterize this equilibrium, the osmotic pressure established across the Donnan membrane is found to be particularly sensitive to intermolecular interactions between the diffusible and ...

journal_title：Biophysical journal

authors： Stell G,Joslin CG

更新日期：1986-11-01 00:00:00

abstract：:We have interrogated the isothermal folding behavior of the DNA i-motif of the human telomere, dC19, and a high-stability i-motif-forming sequence in the promoter of the human DNA repair gene RAD17 using human physiological solution and temperature conditions. We developed a circular-dichroism-spectroscopy-based pH ti...

journal_title：Biophysical journal

authors： Rogers RA,Fleming AM,Burrows CJ

更新日期：2018-04-24 00:00:00

abstract：:The kinetics of porin incorporation into black lipid membranes (BLM) made of phosphatidylinositol (PI) or oxidized cholesterol (Ox Ch) were studied by means of alternating current; the set-up was able to acquire resistance and capacitance simultaneously by means of a mixed double-frequency approach at 1 Hz and 1 KHz, ...

journal_title：Biophysical journal

authors： Gallucci E,Micelli S,Monticelli G

更新日期：1996-08-01 00:00:00