An analytic solution to the Monod-Wyman-Changeux model and all parameters in this model.

abstract：:Actin filaments are semiflexible polymers that display large-scale conformational twisting and bending motions. Modulation of filament bending and twisting dynamics has been linked to regulatory actin-binding protein function, filament assembly and fragmentation, and overall cell motility. The relationship between act...

journal_title：Biophysical journal

authors： De La Cruz EM,Roland J,McCullough BR,Blanchoin L,Martiel JL

更新日期：2010-09-22 00:00:00

abstract：:The effect of transepithelial potential difference (psi) on Na and Cl flux across toad bladder was assessed by measuring isotopic flux between identical media at various values of psi. The contribution of edge damage to ionic permeability was eliminated, resulting in relatively high spontaneous psi (-97 +/-4 mv) and l...

journal_title：Biophysical journal

authors： Walser M

更新日期：1972-04-01 00:00:00

abstract：:Five AHCAPs exhibiting a broad-spectrum of antimicrobial activity, were examined with regard to their action in lipid mixtures with two anionic lipids, PG and CL. We find that all of the peptides studied were capable of promoting the formation of crystalline phases of DMPG in mixtures of DMPG and CL, without prior inc...

journal_title：Biophysical journal

authors： Epand RF,Maloy L,Ramamoorthy A,Epand RM

更新日期：2010-06-02 00:00:00

abstract：:Entropy Sampling Monte Carlo (ESMC) simulations were carried out to study the thermodynamics of the folding transition in the GCN4 leucine zipper (GCN4-lz) in the context of a reduced model. Using the calculated partition functions for the monomer and dimer, and taking into account the equilibrium between the monomer ...

journal_title：Biophysical journal

authors： Mohanty D,Kolinski A,Skolnick J

更新日期：1999-07-01 00:00:00

abstract：:We used the nonlinear Poisson-Boltzmann equation to calculate electrostatic potentials in the aqueous phase adjacent to model phospholipid bilayers containing mixtures of zwitterionic lipids (phosphatidylcholine) and acidic lipids (phosphatidylserine or phosphatidylglycerol). The aqueous phase (relative permittivity, ...

journal_title：Biophysical journal

authors： Peitzsch RM,Eisenberg M,Sharp KA,McLaughlin S

更新日期：1995-03-01 00:00:00

abstract：:Single-stranded junctions/loops are frequently occurring structural motifs in nucleic acid structures. Due to the polyanionic nature of the nucleic acid backbone, metal ions play a crucial role in the loop stability. Here we use the tightly bound ion theory, which can account for the possible ion correlation and ensem...

journal_title：Biophysical journal

authors： Tan ZJ,Chen SJ

更新日期：2008-07-01 00:00:00

abstract：:Na(+) conductance through cloned K(+) channels has previously allowed characterization of inactivation and K(+) binding within the pore, and here we have used Na(+) permeation to study recovery from C-type inactivation in human Kv1.5 channels. Replacing K(+) in the solutions with Na(+) allows complete Kv1.5 inactivati...

journal_title：Biophysical journal

authors： Wang Z,Hesketh JC,Fedida D

更新日期：2000-11-01 00:00:00

abstract：:The vibrational spectroscopy and population dynamics of excited singlet (2(1)Ag), excited triplet (3B u), and the ground (1Ag) electronic states of carotenoids in chromatophores of Chromatium vinosum (mainly spirilloxanthin and rhodopin) and of the same carotenoids in benzene solutions are examined by picosecond time-...

journal_title：Biophysical journal

authors： Hayashi H,Noguchi T,Tasumi M,Atkinson GH

更新日期：1991-07-01 00:00:00

abstract：:Label-free imaging techniques such as differential interference contrast (DIC) allow the observation of cells and large subcellular structures in their native, unperturbed states with minimal exposure to light. The development of robust computational image-analysis routines is vital to quantitative label-free imaging....

journal_title：Biophysical journal

authors： Chaphalkar AR,Jawale YK,Khatri D,Athale CA

更新日期：2021-01-11 00:00:00

abstract：:The interaction with lipids of a synthetic peptide corresponding to the transmembrane domain of influenza hemagglutinin was investigated by means of electron spin resonance. A detailed analysis of the electron spin resonance spectra from spin-labeled phospholipids revealed that the major effect of the peptide on the d...

journal_title：Biophysical journal

authors： Ge M,Freed JH

更新日期：2011-01-05 00:00:00

abstract：:Imaging in any plane other than horizontal in a microscope typically requires a reconstruction from multiple optical slices that significantly decreases the spatial and temporal resolution that can be achieved. This can limit the precision with which molecular events can be detected, for example, at intercellular cont...

journal_title：Biophysical journal

authors： Oddos S,Dunsby C,Purbhoo MA,Chauveau A,Owen DM,Neil MA,Davis DM,French PM

更新日期：2008-11-15 00:00:00

abstract：:The spontaneous folding of two Neisseria outer membrane proteins, opacity-associated (Opa)(60) and Opa(50) into lipid vesicles was investigated by systematically varying bulk and membrane properties. Centrifugal fractionation coupled with sodium dodecyl sulfate polyacrylamide gel electrophoresis mobility assays enable...

journal_title：Biophysical journal

authors： Dewald AH,Hodges JC,Columbus L

更新日期：2011-05-04 00:00:00

abstract：:Skeletal muscle contraction is a canonical example of motor-driven force generation. Despite the long history of research in this topic, a mechanistic explanation of the collective myosin force generation is lacking. We present a theoretical model of muscle contraction based on the conformational movements of individu...

journal_title：Biophysical journal

authors： Lan G,Sun SX

更新日期：2005-06-01 00:00:00

abstract：:A recent analysis of the normal modes of vibration of a ring formed by bringing together and sealing, with or without the addition of twist, the ends of rods that are straight when stress free is taken as the basis for a theory of the statistical thermodynamics of a canonical ensemble of DNA minicircles with specified...

journal_title：Biophysical journal

authors： Tobias I

更新日期：1998-05-01 00:00:00

abstract：:One of the disadvantages of the sucrose-gap method for measuring membrane potentials with extracellular electrodes is a membrane hyperpolarization of the order of 30 to 60 mv, as compared with the resting potential obtained with intracellular microelectrodes in the absence of a sucrose-gap. In the present study the co...

journal_title：Biophysical journal

authors： Blaustein MP,Goldman DE

更新日期：1966-07-01 00:00:00

abstract：:Calcium ions act presynaptically to modulate synaptic strength and to trigger neurotransmitter release. Here we detect stimulus-evoked changes in residual free calcium ([Ca2+]i) in rat cerebellar granule cell presynaptic terminals. Granule cell axons, known as parallel fibers, and their associated boutons, were labele...

journal_title：Biophysical journal

authors： Regehr WG,Atluri PP

更新日期：1995-05-01 00:00:00

abstract：:Fluorescence recovery after photobleaching (FRAP) has been used previously to investigate the kinetics of binding to biological surfaces. The present study adapts and further develops this technique for the quantification of mass transport and reaction parameters in bulk media. The technique's ability to obtain the bu...

journal_title：Biophysical journal

authors： Kaufman EN,Jain RK

更新日期：1991-09-01 00:00:00

abstract：:The increasingly widespread use of parametric mathematical models to describe biological systems means that the ability to infer model parameters is of great importance. In this study, we consider parameter inferability in nonlinear ordinary differential equation models that undergo a bifurcation, focusing on a simple...

journal_title：Biophysical journal

authors： Kirk PD,Toni T,Stumpf MP

更新日期：2008-07-01 00:00:00

abstract：:To investigate the force generation properties of Chlamydomonas axonemal inner-arm dyneins in response to external force, we analyzed microtubule gliding on dynein-coated surfaces under shear flow. When inner-arm dynein c was used, microtubule translocation in the downstream direction accelerated with increasing flow ...

journal_title：Biophysical journal

authors： Kikushima K,Kamiya R

更新日期：2009-09-16 00:00:00

abstract：:Sarcoplasmic reticulum (SR) Ca2+-ATPase (SERCA) and phospholamban (PLB) are essential for intracellular Ca2+ transport in myocytes. Ca2+-dependent activation of SERCA-PLB provides a control function that regulates cytosolic and SR Ca2+ levels. Although experimental and computational studies alone have led to a greater...

journal_title：Biophysical journal

authors： Raguimova ON,Aguayo-Ortiz R,Robia SL,Espinoza-Fonseca LM

更新日期：2020-11-03 00:00:00

abstract：:The ionophore properties of magainin I, an antimicrobial and amphipathic peptide from the skin of Xenopus, were investigated in planar lipid bilayers. Circular dichroism studies, performed comparatively with alamethicin, in small or large unilamellar phospholipidic vesicles, point to a smaller proportion of alpha-heli...

journal_title：Biophysical journal

authors： Duclohier H,Molle G,Spach G

更新日期：1989-11-01 00:00:00

abstract：:The insertion of myelin basic protein into microemulsion droplets of sodium bis (2-ethylhexyl) sulfosuccinate (AOT) has been studied by quasi-elastic light scattering. Measurements were made at both low and high molar ratios of water to surfactant, as a function of protein occupancy. The hydrodynamic radii of filled a...

journal_title：Biophysical journal

authors： Chatenay D,Urbach W,Cazabat AM,Vacher M,Waks M

更新日期：1985-12-01 00:00:00

abstract：:Morphine spin labeled on the phenolic hydroxy group has been prepared using commercially available reagents and characterized by thin layer chromatography, mass spectroscopy, and electron spin resonance spectroscopy. It has been shown that morphine modified in this way retains some opiate activity, does not pass throu...

journal_title：Biophysical journal

authors： Copeland ES,Boykin ME,Kelley JA,Kullberg MP

更新日期：1975-11-01 00:00:00

abstract：:The possibility that the chlorophyll (chl) ring distortions observed in the crystal structures of chl-protein complexes are involved in the transition energy modulation, giving rise to the spectral forms, is investigated. The out-of-plane chl-macrocycle distortions are described using an orthonormal set of deformation...

journal_title：Biophysical journal

authors： Zucchelli G,Brogioli D,Casazza AP,Garlaschi FM,Jennings RC

更新日期：2007-09-15 00:00:00

abstract：:We describe a statistical mechanical model for lipid-cholesterol mixtures in the P beta' (ripple) phase of lipid bilayers. The model is a simple extension of an earlier model for the ripple phase in pure lipid bilayers. The extension consists of adding a degree of freedom to allow for the occupation of underlying latt...

journal_title：Biophysical journal

authors： Scott HL,McCullough WS

更新日期：1993-05-01 00:00:00

abstract：:The photocycle of the photophobic receptor from Natronobacterium pharaonis, NpSRII, is studied by static and time-resolved step-scan Fourier transform infrared spectroscopy. Both low-temperature static and time-resolved spectra resolve a K-like intermediate, and the corresponding spectra show little difference within ...

journal_title：Biophysical journal

authors： Hein M,Wegener AA,Engelhard M,Siebert F

更新日期：2003-02-01 00:00:00

abstract：:Identifying the basic module of enzymatic amplification as an irreversible cycle of messenger activation/deactivation by a "push-pull" pair of opposing enzymes, we analyze it in terms of gain, bandwidth, noise, and power consumption. The enzymatic signal transduction cascade is viewed as an information channel, the de...

journal_title：Biophysical journal

authors： Detwiler PB,Ramanathan S,Sengupta A,Shraiman BI

更新日期：2000-12-01 00:00:00

abstract：:BP100 (KKLFKKILKYL-NH(2)) is a short cecropin A-melittin hybrid peptide, obtained through a combinatorial chemistry approach, which is highly effective in inhibiting both the in vitro and in vivo growth of economically important plant pathogenic Gram-negatives. The intrinsic Tyr fluorescence of BP100 was taken advanta...

journal_title：Biophysical journal

authors： Ferre R,Melo MN,Correia AD,Feliu L,Bardají E,Planas M,Castanho M

更新日期：2009-03-04 00:00:00

abstract：:The effects of lysolipids on phospholipid layer curvature and bending elasticity were examined using x-ray diffraction and the osmotic stress method. Lysolipids with two different head groups, phosphatidylcholine (PC) and phosphatidylethanolamine (PE), and differing hydrocarbon chains were mixed with the hexagonal-for...

journal_title：Biophysical journal

authors： Fuller N,Rand RP

更新日期：2001-07-01 00:00:00

abstract：:Actin polymerization is typically initiated at specific sites in a cell by membrane-bound protein complexes, and the resulting structures are involved in specialized cellular functions, such as migration, particle uptake, or mitotic division. Here we analyze the potential of the actin system to self-organize into wave...

journal_title：Biophysical journal

authors： Bretschneider T,Anderson K,Ecke M,Müller-Taubenberger A,Schroth-Diez B,Ishikawa-Ankerhold HC,Gerisch G

更新日期：2009-04-08 00:00:00