Oleoylethanolamide-based lyotropic liquid crystals as vehicles for delivery of amino acids in aqueous environment.

abstract：:A newly developed water-soluble phosphor suitable for measuring oxygen pressure in the blood (Green 2W) was used for noninvasive, in vivo imaging of oxygen distribution in the vascular systems of mice. Oxygen quenches the phosphorescence of Green 2W, measured in the presence of 2% albumin, according to the Stern-volme...

journal_title：Biophysical journal

authors： Vinogradov SA,Lo LW,Jenkins WT,Evans SM,Koch C,Wilson DF

更新日期：1996-04-01 00:00:00

abstract：:In multicellular organisms, single-fluorophore imaging is obstructed by high background. To achieve a signal/noise ratio conducive to single-molecule imaging, we adapted reflected light-sheet microscopy (RLSM) to image highly opaque late-stage Drosophila embryos. Alignment steps were modified by means of commercially ...

journal_title：Biophysical journal

authors： Greiss F,Deligiannaki M,Jung C,Gaul U,Braun D

更新日期：2016-02-23 00:00:00

abstract：:Förster resonance energy transfer (FRET) has become an important tool for analyzing different aspects of interactions among biological macromolecules in their native environments. FRET analysis has also been successfully applied to study the spatiotemporal regulation of various cellular processes using genetically enc...

journal_title：Biophysical journal

authors： Zeug A,Woehler A,Neher E,Ponimaskin EG

更新日期：2012-11-07 00:00:00

abstract：:Fatty acid binding proteins play an important role in the transportation of fatty acids. Despite intensive studies, how fatty acids enter the protein cavity for binding is still controversial. Here, a gap-closed variant of human intestinal fatty acid binding protein was generated by mutagenesis, in which the gap is lo...

journal_title：Biophysical journal

authors： Xiao T,Lu Y,Fan JS,Yang D

更新日期：2020-01-21 00:00:00

abstract：:Lateral mobility of molecules on cell membranes has been recently studied by fluorescence photobleaching recovery (FPR) techniques. The interpretation of these results in terms of diffusion along the membranes is based on the assumption that the surface is planar, although biological membranes may have blebs and micro...

journal_title：Biophysical journal

authors： Aizenbud BM,Gershon ND

更新日期：1982-06-01 00:00:00

abstract：:We describe a puffing method for changing solutions near one surface of lipid bilayers that allows simultaneous measurement of channel activity and extent of solution change at the bilayer surface. Ion adsorption to the lipid headgroups and screening of the bilayer surface charge by mobile ions provided a convenient p...

journal_title：Biophysical journal

authors： Laver DR,Curtis BA

更新日期：1996-08-01 00:00:00

abstract：:Many complex cellular processes from mitosis to cell motility depend on the ability of the cytoskeleton to generate force. Force-generating systems that act on elastic cytoskeletal elements are prone to oscillating instabilities. In this work, we have measured spontaneous shape and movement oscillations in motile fish...

journal_title：Biophysical journal

authors： Barnhart EL,Allen GM,Jülicher F,Theriot JA

更新日期：2010-03-17 00:00:00

abstract：:Viral infection begins with the binding of a virus to a specific target on the surface of the host cell, followed by viral genome delivery into the host and a continuation of the infection process. Before binding occurs, the virus must first find its receptor by a process whose details are largely unknown. We applied ...

journal_title：Biophysical journal

authors： Rothenberg E,Sepúlveda LA,Skinner SO,Zeng L,Selvin PR,Golding I

更新日期：2011-06-22 00:00:00

abstract：:The electric quadrupole moment of the deuterium nucleus provides a nuclear magnetic resonance (NMR) probe of electric field gradients, and thereby of organization of tissue water. 8-17% of H(2)O in rat muscle and brain was replaced by D(2)O from 50% deuterated drinking water. The peak height of the steady-state NMR sp...

journal_title：Biophysical journal

authors： Cope FW

更新日期：1969-03-01 00:00:00

abstract：:The outer membrane porin OmpF from Escherichia coli has been reconstituted into lipid bilayers of defined composition, and fluorescence spectroscopy is used to characterize its interaction with the surrounding lipid. OmpF is a trimer within the membrane. It contains two Trp residues per monomer, Trp(214) at the lipid-...

journal_title：Biophysical journal

authors： O'Keeffe AH,East JM,Lee AG

更新日期：2000-10-01 00:00:00

abstract：:Halorhodopsin (HR), the light-driven chloride transport pigment of Halobacterium halobium, was bleached and reconstituted with retinal analogues with the pi electron system interrupted at different locations (dihydroretinals). The absorption maxima of the artificial pigments formed with the dihydroretinals are found t...

journal_title：Biophysical journal

authors： Lanyi JK,Zimányi L,Nakanishi K,Derguini F,Okabe M,Honig B

更新日期：1988-02-01 00:00:00

abstract：:The solution-scattering profiles of macromolecules are significantly affected by the thermal motions of their atoms, especially at wide scattering angles, even when only a single conformational state is significantly populated in solution. Here it is shown that the impact thermal motions have on the molecular componen...

journal_title：Biophysical journal

authors： Moore PB

更新日期：2014-04-01 00:00:00

abstract：:Chlamydomonas exhibits a sequence of a photoreceptor current and two flagellar currents upon stimulation with bright green flashes. The currents are thought to be a prerequisite for the well-known photophobic responses. In the preceding paper, we analyzed the kinetics of these currents and their dependence on extracel...

journal_title：Biophysical journal

authors： Nonnengässer C,Holland EM,Harz H,Hegemann P

更新日期：1996-02-01 00:00:00

abstract：:The extracellular space of the brain is the heterogeneous porous medium formed by the spaces between the brain cells. Diffusion in this interstitial space is the mechanism by which glucose and oxygen are delivered to the brain cells from the vascular system. It is also a medium for the transport of certain information...

journal_title：Biophysical journal

authors： Nandigam RK,Kroll DM

更新日期：2007-05-15 00:00:00

abstract：:Propagation speed of an impulse is influenced by previous activity. A pulse following its predecessor too closely may travel more slowly than a solitary pulse. In contrast, for some range of interspike intervals, a pulse may travel faster than normal because of a possible superexcitable phase of its predecessor's wake...

journal_title：Biophysical journal

authors： Miller RN,Rinzel J

更新日期：1981-05-01 00:00:00

abstract：:The mechanism for transport of divalent cations across phospholipid bilayers by the ionophore A23187 was investigated. The intrinsic fluorescence of the ionophore was used in equilibrium and rapid-mixing experiments as an indicator of ionophore environment and complexation with divalent cations. The neutral (protonate...

journal_title：Biophysical journal

authors： Kolber MA,Haynes DH

更新日期：1981-11-01 00:00:00

abstract：:A Ca(2+) spark arises when a cluster of sarcoplasmic reticulum (SR) channels (ryanodine receptors or RyRs) opens to release calcium in a locally regenerative manner. Normally triggered by Ca(2+) influx across the sarcolemmal or transverse tubule membrane neighboring the cluster, the Ca(2+) spark has been shown to be t...

journal_title：Biophysical journal

authors： Sobie EA,Dilly KW,dos Santos Cruz J,Lederer WJ,Jafri MS

更新日期：2002-07-01 00:00:00

abstract：:Scanning ion conductance microscopy (SICM) is a super-resolution live imaging technique that uses a glass nanopipette as an imaging probe to produce three-dimensional (3D) images of cell surface. SICM can be used to analyze cell morphology at nanoscale, follow membrane dynamics, precisely position an imaging nanopipet...

journal_title：Biophysical journal

authors： Shevchuk A,Tokar S,Gopal S,Sanchez-Alonso JL,Tarasov AI,Vélez-Ortega AC,Chiappini C,Rorsman P,Stevens MM,Gorelik J,Frolenkov GI,Klenerman D,Korchev YE

更新日期：2016-05-24 00:00:00

abstract：:In skeletal muscle, excitation-contraction coupling involves the activation of dihydropyridine receptors (DHPR) and type-1 ryanodine receptors (RyR1) to produce depolarization-dependent sarcoplasmic reticulum Ca²⁺ release via orthograde signaling. Another form of DHPR-RyR1 communication is retrograde signaling, in whi...

journal_title：Biophysical journal

authors： Olojo RO,Hernández-Ochoa EO,Ikemoto N,Schneider MF

更新日期：2011-05-18 00:00:00

abstract：:Skeletal and heart muscle excitability is based upon the pool of available sodium channels as determined by both fast and slow inactivation. Slow inactivation in hH1 sodium channels significantly differs from slow inactivation in hSkM1. The beta(1)-subunit modulates fast inactivation in human skeletal sodium channels ...

journal_title：Biophysical journal

authors： Vilin YY,Makita N,George AL Jr,Ruben PC

更新日期：1999-09-01 00:00:00

abstract：:We show prolonged contraction of permeabilized muscle fibers of the frog during which structural order, as judged from low-angle x-ray diffraction, was preserved by means of partial cross-linking of the fibers using the zero-length cross-linker 1-ethyl-3-[3-dimethylamino)propyl]carbodiimide. Ten to twenty percent of t...

journal_title：Biophysical journal

authors： Bershitsky S,Tsaturyan A,Bershitskaya O,Mashanov G,Brown P,Webb M,Ferenczi MA

更新日期：1996-09-01 00:00:00

abstract：:Five AHCAPs exhibiting a broad-spectrum of antimicrobial activity, were examined with regard to their action in lipid mixtures with two anionic lipids, PG and CL. We find that all of the peptides studied were capable of promoting the formation of crystalline phases of DMPG in mixtures of DMPG and CL, without prior inc...

journal_title：Biophysical journal

authors： Epand RF,Maloy L,Ramamoorthy A,Epand RM

更新日期：2010-06-02 00:00:00

abstract：:Time-resolved dielectric loss (TRDL) measurements are reported for the photochemical excitation of bacteriorhodopsin (bR) in solid films of Halobacterium halobium purple membranes. These measurements provide an independent confirmation for the existence of an important component of charge separation in these membranes...

journal_title：Biophysical journal

authors： McIntosh AR,Boucher F

更新日期：1991-07-01 00:00:00

abstract：:The optical scattering coefficient of a dilute, well-solubilized eumelanin solution has been accurately measured as a function of incident wavelength, and found to contribute <6% of the total optical attenuation between 210 and 325 nm. At longer wavelengths (325-800 nm), the scattering was less than the minimum sensit...

journal_title：Biophysical journal

authors： Riesz J,Gilmore J,Meredith P

更新日期：2006-06-01 00:00:00

abstract：:Embryonic and adult fibroblasts can be returned to pluripotency by the expression of reprogramming genes. Multiple lines of evidence suggest that these human induced pluripotent stem (hiPS) cells and human embryonic stem (hES) cells are behaviorally, karyotypically, and morphologically similar. Here we sought to deter...

journal_title：Biophysical journal

authors： Daniels BR,Hale CM,Khatau SB,Kusuma S,Dobrowsky TM,Gerecht S,Wirtz D

更新日期：2010-12-01 00:00:00

abstract：:Electron cryomicroscopy of rotor complexes of the Salmonella typhimurium flagellar motor, overproduced in a nonmotile Escherichia coli host, has revealed a variation in subunit symmetry of the cytoplasmic ring (C ring) module. C rings with subunit symmetries ranging from 31 to 38 were found. They formed a Gaussian dis...

journal_title：Biophysical journal

authors： Young HS,Dang H,Lai Y,DeRosier DJ,Khan S

更新日期：2003-01-01 00:00:00

abstract：:In the absence of atomic structures of high-density lipoproteins in their lipid-bound states, many molecular models have been produced based on experimental data. Using molecular dynamics, we show that a recently proposed "solar-flares" model of discoidal high-density lipoprotein is implausible. Our simulations show a...

journal_title：Biophysical journal

authors： Shih AY,Sligar SG,Schulten K

更新日期：2008-06-01 00:00:00

abstract：:We describe two enhancements of the planar bilayer recording method which enable low-noise recordings of single-channel currents activated by voltage steps in planar bilayers formed on apertures in partitions separating two open chambers. First, we have refined a simple and effective procedure for making small bilayer...

journal_title：Biophysical journal

authors： Wonderlin WF,Finkel A,French RJ

更新日期：1990-08-01 00:00:00

abstract：:Using the simple argument based on irreversible thermodynamics and the Gouy-Chapman theory of the double layer, we show that the equilibrium distribution of charged lipid molecules between the two surfaces of a bilayer is asymmetric if the two solutions bathing the surfaces have the same ionic strength but contain ion...

journal_title：Biophysical journal

authors： McQuarrie DA,Mulás P

更新日期：1977-02-01 00:00:00

abstract：:Spontaneous conversion of beta-amyloid peptide (Abeta) from soluble monomer to insoluble fibril may underlie the neurodegeneration associated with Alzheimer's disease. A complete description of Abeta self-association kinetics requires identification of the oligomeric species present and the pathway of association, as ...

journal_title：Biophysical journal

authors： Pallitto MM,Murphy RM

更新日期：2001-09-01 00:00:00