Diffusion of molecules on biological membranes of nonplanar form. A theoretical study.

abstract：:Under the assumption that vesicles are the anatomic correlate of quantal release, the forces governing the movement of synaptic vesicles inside neurons are analyzed. Semiquantitative calculations are presented to show that a diffuse layer field penetrates a few Debye lengths into the axoplasm. This field binds tightly...

journal_title：Biophysical journal

authors： Remler MP

更新日期：1973-02-01 00:00:00

abstract：:Steady-state and time-resolved fluorescence spectroscopy was used to follow the local and global changes in structure and dynamics during chemical and thermal denaturation of unlabeled human serum albumin (HSA) and HSA with an acrylodan moiety bound to Cys34. Acrylodan fluorescence was monitored to obtain information ...

journal_title：Biophysical journal

authors： Flora K,Brennan JD,Baker GA,Doody MA,Bright FV

更新日期：1998-08-01 00:00:00

abstract：:Mislocalization and aggregation of the huntingtin protein are related to Huntington's disease. Its first exon-more specifically the first 17 amino acids (Htt17)-is crucial for the physiological and pathological functions of huntingtin. It regulates huntingtin's activity through posttranslational modifications and serv...

journal_title：Biophysical journal

authors： Côté S,Binette V,Salnikov ES,Bechinger B,Mousseau N

更新日期：2015-03-10 00:00:00

abstract：:Two-dimensional electronic chirality-induced signals of excitons in the photosynthetic Fenna-Matthews-Olson complex from two species of green sulfur bacteria (Chlorobium tepidum and Prosthecochloris aestuarii) are compared. The spectra are predicted to provide sensitive probes of local protein environment of the const...

journal_title：Biophysical journal

authors： Voronine DV,Abramavicius D,Mukamel S

更新日期：2008-11-15 00:00:00

abstract：:We investigated whether transient step reductions in divalent cations would produce detectable changes in neuronal excitability similar to those reported in the total absence of divalent cations. Using cultured chick dorsal root ganglion cells as a model system, our results indicate that a step reduction in divalent c...

journal_title：Biophysical journal

authors： Hablitz JJ,Heinemann U,Lux HD

更新日期：1986-10-01 00:00:00

abstract：:A free radical probe was introduced into single barnacle muscle cells, and its freedom of motion inferred from the spin resonance spectra. The probe reported an average local viscosity of 5-10 cp compared with 1 cp for pure water. From a comparison of the temperature dependence of the probe's tumbling rate in model aq...

journal_title：Biophysical journal

authors： Sachs F,Latorre R

更新日期：1974-04-01 00:00:00

abstract：:Gangliosides are a class of glycosphingolipids (GSLs) with amphiphilic character that are found at the outer leaflet of the cell membranes, where their ability to organize into special domains makes them vital cell membrane components. However, a molecular understanding of GSL-rich membranes in terms of their clustere...

journal_title：Biophysical journal

authors： Patel DS,Park S,Wu EL,Yeom MS,Widmalm G,Klauda JB,Im W

更新日期：2016-11-01 00:00:00

abstract：:Immunocytochemical, biochemical, and molecular genetic studies indicate that apolipoprotein E (apoE) plays an important role in the process of amyloidogenesis-beta. However, there is still no clear translation of these data into the pathogenesis of amyloidosis-beta. Previous studies demonstrated sodium dodecyl sulfate...

journal_title：Biophysical journal

authors： Golabek AA,Kida E,Walus M,Perez C,Wisniewski T,Soto C

更新日期：2000-08-01 00:00:00

abstract：:Although light microscopy and three-dimensional image analysis have made considerable progress during the last decade, it is still challenging to analyze the genome nano-architecture of specific gene domains in three-dimensional cell nuclei by fluorescence microscopy. Here, we present for the first time chromatin comp...

journal_title：Biophysical journal

authors： Hildenbrand G,Rapp A,Spöri U,Wagner C,Cremer C,Hausmann M

更新日期：2005-06-01 00:00:00

abstract：:The molecular weight of single-stranded DNA from the slime mold Physarum polycephalum has been determined by alkaline gradient centrifugation. The average molecular weight during DNA synthesis ( approximately 1.5 x 10(7) D) is less than that observed in nonsynthetic periods ( approximately 4 x 10(7) D). On the basis o...

journal_title：Biophysical journal

authors： McGrath RA,Williams RW

更新日期：1967-05-01 00:00:00

abstract：:Hybrid global optimization methods attempt to combine the beneficial features of two or more algorithms, and can be powerful methods for solving challenging nonconvex optimization problems. In this paper, novel classes of hybrid global optimization methods, termed alternating hybrids, are introduced for application as...

journal_title：Biophysical journal

authors： Klepeis JL,Pieja MJ,Floudas CA

更新日期：2003-02-01 00:00:00

abstract：:We describe a combined use of experimental and simulation techniques to configure side chains in a coiled coil structure. As already demonstrated in a previous work, x-ray diffraction patterns from hard alpha-keratin fibers in the 5.15 A meridian zone reflect the global configuration of the chi(1) dihedral angle of th...

journal_title：Biophysical journal

authors： Briki F,Doucet J,Etchebest C

更新日期：2002-10-01 00:00:00

abstract：:The formation and function of the mitotic spindle depends upon force generation by multiple molecular motors and by the dynamics of microtubules, but how these force-generating mechanisms relate to one another is unclear. To address this issue we have modeled the separation of spindle poles as a function of time durin...

journal_title：Biophysical journal

authors： Cytrynbaum EN,Scholey JM,Mogilner A

更新日期：2003-02-01 00:00:00

abstract：:Uracil DNA Glycosylase (UNG2) is the primary enzyme in humans that prevents the stable incorporation of deoxyuridine monophosphate into DNA in the form of U/A basepairs. During S-phase, UNG2 remains associated with the replication fork through its interactions with two proteins, Proliferating Cell Nuclear Antigen (PCN...

journal_title：Biophysical journal

authors： Weiser BP,Stivers JT,Cole PA

更新日期：2017-07-25 00:00:00

abstract：:The cellular cytoplasm is a complex, heterogeneous environment (both spatially and temporally) that exhibits viscoelastic behavior. To further develop our quantitative insight into cellular transport, we analyze data sets of mRNA molecules fluorescently labeled with MS2-GFP tracked in real time in live Escherichia col...

journal_title：Biophysical journal

authors： Lampo TJ,Stylianidou S,Backlund MP,Wiggins PA,Spakowitz AJ

更新日期：2017-02-07 00:00:00

abstract：:It is widely assumed that the decay of fluorescence in photosynthetic systems can be described as a sum of exponential components and that the amplitude of each component is directly related to the absorption cross-section of the antenna pigments coupled to the fluorescing species. We present exact calculations of exc...

journal_title：Biophysical journal

authors： Jean JM,Chan CK,Fleming GR,Owens TG

更新日期：1989-12-01 00:00:00

abstract：:We study the migration of chemotactic wild-type Escherichia coli populations in semisolid (soft) agar in the concentration range C = 0.15-0.5% (w/v). For C≲0.35%, expanding bacterial colonies display characteristic chemotactic rings. At C = 0.35%, however, bacteria migrate as broad circular bands rather than sharp rin...

journal_title：Biophysical journal

authors： Croze OA,Ferguson GP,Cates ME,Poon WC

更新日期：2011-08-03 00:00:00

abstract：:Analyses of receptor-ligand interactions are important to the understanding of cellular adhesion. Traditional methods of measuring the three-dimensional (3D) dissociation constant (Kd) require at least one of the molecular species in solution and hence cannot be directly applied to the case of cell adhesion. We descri...

journal_title：Biophysical journal

authors： Piper JW,Swerlick RA,Zhu C

更新日期：1998-01-01 00:00:00

abstract：:Herein, we consider the process of force development along the adhesome within cell focal adhesions. Our model adhesome consists of the actin cytoskeleton-vinculin-talin-integrin-ligand-extracellular matrix-substrate force train. We specifically consider the effects of substrate stiffness on the force levels expected ...

journal_title：Biophysical journal

authors： Asaro RJ,Lin K,Zhu Q

更新日期：2019-11-05 00:00:00

abstract：:We report applications of two-photon excitation fluorescence (2PEF) microscopy with subdiffraction-limit resolution for green-fluorescent-protein-tagged cell imaging. The microscope integrates 2PEF microscopy and stimulated emission depletion microscopy in one microscope that has the benefits of both techniques: intri...

journal_title：Biophysical journal

authors： Li Q,Wu SS,Chou KC

更新日期：2009-12-16 00:00:00

abstract：:We have measured the kinetics of adsorption of small (12.5-nm radius) unilamellar vesicles onto SiO2, oxidized gold, and a self-assembled monolayer of methyl-terminated thiols, using a quartz crystal microbalance (QCM). Simultaneous measurements of the shift in resonant frequency and the change in energy dissipation a...

journal_title：Biophysical journal

authors： Keller CA,Kasemo B

更新日期：1998-09-01 00:00:00

abstract：:We introduce here a novel Monte Carlo simulation method for studying the interactions of hydrophobic peptides with lipid membranes. Each of the peptide's amino acids is represented as two interaction sites: one corresponding to the backbone alpha-carbon and the other to the side chain, with the membrane represented as...

journal_title：Biophysical journal

authors： Kessel A,Shental-Bechor D,Haliloglu T,Ben-Tal N

更新日期：2003-12-01 00:00:00

abstract：:Magic-angle spinning nuclear magnetic resonance is well suited for the study of membrane proteins in the nativelike lipid environment. However, the natural cellular membrane is invariably more complex than the proteoliposomes most often used for solid-state NMR (SSNMR) studies, and differences may affect the structure...

journal_title：Biophysical journal

authors： Ward ME,Wang S,Munro R,Ritz E,Hung I,Gor'kov PL,Jiang Y,Liang H,Brown LS,Ladizhansky V

更新日期：2015-04-07 00:00:00

abstract：:During postnatal development, major changes in mechanical properties of skeletal muscle occur. We investigated passive properties of skeletal muscle in mice and rabbits that varied in age from 1 day to approximately 1 year. Neonatal skeletal muscle expressed large titin isoforms directly after birth, followed by a gra...

journal_title：Biophysical journal

authors： Ottenheijm CA,Knottnerus AM,Buck D,Luo X,Greer K,Hoying A,Labeit S,Granzier H

更新日期：2009-10-21 00:00:00

abstract：:Subdenaturing concentrations of guanidine hydrochloride (GdnHCl) stabilize proteins. For ferrocytochrome c the stabilization is detected at subglobal level with no measured change in global stability. These deductions are made by comparing observed rates of thermally driven ferrocytochrome cHCO reactions with global u...

journal_title：Biophysical journal

authors： Kumar R,Prabhu NP,Yadaiah M,Bhuyan AK

更新日期：2004-10-01 00:00:00

abstract：:Conditions for which a ligand reversibly bound to a cell surface dissociates and then rebinds to the surface have been theoretically examined. The coupled differential equations that describe reaction at the interface between sites on a plane and three-dimensional solution have been described previously (Thompson, N. ...

journal_title：Biophysical journal

authors： Lagerholm BC,Thompson NL

更新日期：1998-03-01 00:00:00

abstract：:Diffusional encounters between a dumbbell model of a cleft enzyme and a dumbbell model of an elongated ligand are simulated by Brownian dynamics. The simulations take into account electrostatic and hydrodynamic interactions between the molecules. It is shown that the primary effect of inclusion of hydrodynamic interac...

journal_title：Biophysical journal

authors： Antosiewicz J,McCammon JA

更新日期：1995-07-01 00:00:00

abstract：:Fundamental mechanisms governing cell size control and homeostasis are still poorly understood. The relationship between sizes at division and birth in single cells is used as a metric to categorize the basis of size homeostasis. Cells dividing at a fixed size regardless of birth size (sizer) are expected to show a di...

journal_title：Biophysical journal

authors： Facchetti G,Knapp B,Chang F,Howard M

更新日期：2019-11-05 00:00:00

abstract：:Two modes of behavior of single human red cells in a shear field have been described. It is known that in low viscosity media and at shear rates less than 20 s-1, the cells rotate with a periodically varying angular velocity, in accord with the theory of Jeffery (1922) for oblate spheroids. In media of viscosity great...

journal_title：Biophysical journal

authors： Bitbol M

更新日期：1986-05-01 00:00:00

abstract：:Ceramides have been implicated in the initiation of apoptosis by permeabilizing the mitochondrial outer membrane to small proteins, including cytochrome c. In addition, ceramides were shown to form large metastable channels in planar membranes and liposomes, indicating that these lipids permeabilize membranes directly...

journal_title：Biophysical journal

authors： Anishkin A,Sukharev S,Colombini M

更新日期：2006-04-01 00:00:00