Subdiffraction-limit two-photon fluorescence microscopy for GFP-tagged cell imaging.

abstract：:Many membrane-bound molecules in cells form small clusters. It has been hypothesized that these clusters convert an analog extracellular signal into a digital intracellular signal and that this conversion increases signaling fidelity. However, the mechanism by which clusters digitize a signal and the subsequent effect...

journal_title：Biophysical journal

authors： Roob E 3rd,Trendel N,Rein Ten Wolde P,Mugler A

更新日期：2016-04-12 00:00:00

abstract：:Cationic lipid-DNA (CL-DNA) complexes are abundantly used in nonviral gene therapy clinical applications. Surface functionality is the next step in developing these complexes as competent, target-specific gene carriers. Poly(ethylene glycol) (PEG) is the natural choice to serve as a protective coat or act as a tether ...

journal_title：Biophysical journal

authors： Martin-Herranz A,Ahmad A,Evans HM,Ewert K,Schulze U,Safinya CR

更新日期：2004-02-01 00:00:00

abstract：:The structure of the unusually long (∼100 amino-acid residues) N-terminal domain of the light-harvesting protein CP29 of plants is not defined in the crystal structure of this membrane protein. We studied the N-terminus using two electron paramagnetic resonance (EPR) approaches: the rotational diffusion of spin labels...

journal_title：Biophysical journal

authors： Shabestari MH,Wolfs CJ,Spruijt RB,van Amerongen H,Huber M

更新日期：2014-03-18 00:00:00

abstract：:Integrin-mediated adhesion of circulating neutrophils to endothelium during inflammation involves multiple adhesion molecules on both neutrophils and endothelium. Most studies of neutrophil adhesion have focused on adhesion to ICAM-1 (mediated by beta(2) integrins), but interaction with the endothelial ligand vascular...

journal_title：Biophysical journal

authors： Lomakina EB,Waugh RE

更新日期：2009-01-01 00:00:00

abstract：:The Jun a 3 protein from mountain cedar (Juniperus ashei) pollen, a member of group 5 of the family of plant pathogenesis-related proteins (PR-proteins), reacts with serum IgE from patients with cedar hypersensitivity. We used the crystal structures of two other proteins of this group, thaumatin and an antifungal prot...

journal_title：Biophysical journal

authors： Soman KV,Midoro-Horiuti T,Ferreon JC,Goldblum RM,Brooks EG,Kurosky A,Braun W,Schein CH

更新日期：2000-09-01 00:00:00

abstract：:Time-resolved dielectric loss (TRDL) measurements are reported for the photochemical excitation of bacteriorhodopsin (bR) in solid films of Halobacterium halobium purple membranes. These measurements provide an independent confirmation for the existence of an important component of charge separation in these membranes...

journal_title：Biophysical journal

authors： McIntosh AR,Boucher F

更新日期：1991-07-01 00:00:00

abstract：:With the aim of understanding the relation between structure and gating of CNGA1 channels from bovine rod, an extensive cysteine scanning mutagenesis was performed. Each residue from Phe-375 to Val-424 was mutated into a cysteine one at a time and the modification caused by various sulfhydryl reagents was analyzed. Th...

journal_title：Biophysical journal

authors： Nair AV,Mazzolini M,Codega P,Giorgetti A,Torre V

更新日期：2006-05-15 00:00:00

abstract：:Excitation-contraction coupling in atrial cells is mediated by calcium (Ca) signaling between L-type Ca channels and Ryanodine receptors that occurs mainly at the cell boundary. This unique architecture dictates essential aspects of Ca signaling under both normal and diseased conditions. In this study we apply laser s...

journal_title：Biophysical journal

authors： Shiferaw Y,Aistrup GL,Wasserstrom JA

更新日期：2017-08-08 00:00:00

abstract：:The primary drivers of yeast endocytosis are actin polymerization and curvature-generating proteins, such as clathrin and BAR domain proteins. Previous work has indicated that these factors may not be capable of generating the forces necessary to overcome turgor pressure. Thus local reduction of the turgor pressure, v...

journal_title：Biophysical journal

authors： Scher-Zagier JK,Carlsson AE

更新日期：2016-12-20 00:00:00

abstract：:The giant protein titin is responsible for the elasticity of nonactivated muscle sarcomeres. Titin-based passive stiffness in myocardium is modulated by titin-isoform switching and protein-kinase (PK)A- or PKG-dependent titin phosphorylation. Additional modulatory effects on titin stiffness may arise from disulfide bo...

journal_title：Biophysical journal

authors： Grützner A,Garcia-Manyes S,Kötter S,Badilla CL,Fernandez JM,Linke WA

更新日期：2009-08-05 00:00:00

abstract：:Morphine spin labeled on the phenolic hydroxy group has been prepared using commercially available reagents and characterized by thin layer chromatography, mass spectroscopy, and electron spin resonance spectroscopy. It has been shown that morphine modified in this way retains some opiate activity, does not pass throu...

journal_title：Biophysical journal

authors： Copeland ES,Boykin ME,Kelley JA,Kullberg MP

更新日期：1975-11-01 00:00:00

abstract：:The mechanisms by which extracellular electric field stimuli induce the (re)excitation of cardiac cells in various stages of refractoriness are still not well understood. We modeled the interactions between an isolated cardiac cell and imposed extracellular electric fields to determine the mechanisms by which relative...

journal_title：Biophysical journal

authors： Fishler MG,Sobie EA,Thakor NV,Tung L

更新日期：1996-03-01 00:00:00

abstract：:In this article, we demonstrate the new method of pulsed interleaved excitation (PIE), which can be used to extend the capabilities of multiple-color fluorescence imaging, fluorescence cross-correlation spectroscopy (FCCS), and single-pair fluorescence resonance energy transfer (spFRET) measurements. In PIE, multiple ...

journal_title：Biophysical journal

authors： Müller BK,Zaychikov E,Bräuchle C,Lamb DC

更新日期：2005-11-01 00:00:00

abstract：:We have determined directly the effects of the inhibitory peptide phospholamban (PLB) on the rotational dynamics of the calcium pump (Ca-ATPase) of cardiac sarcoplasmic reticulum (SR). This was accomplished by comparing mouse ventricular SR, which has PLB levels similar to those found in other mammals, with mouse atri...

journal_title：Biophysical journal

authors： Voss JC,Mahaney JE,Jones LR,Thomas DD

更新日期：1995-05-01 00:00:00

abstract：:Crystals of ion-free gramicidin A (P212121: a = 24.61, b = 32.28, c = 32.52) have been investigated using neutron diffraction. A difference analysis of crystals soaked in ethanol/H2O as opposed to ethanol-d6/D2O has led to single isomorphous replacement Fourier projections of the structure at 5-A resolution. The grami...

journal_title：Biophysical journal

authors： Koeppe RE 2nd,Schoenborn BP

更新日期：1984-03-01 00:00:00

abstract：:Control of organelle abundance is a fundamental unsolved problem in cell biology. Mechanisms for number control have been proposed in which organelle assembly is actively increased or decreased to compensate for deviations from a set-point, but such phenomena have not been experimentally verified. In this report we ex...

journal_title：Biophysical journal

authors： Marshall WF

更新日期：2007-09-01 00:00:00

abstract：:Members of the epidermal growth factor receptor family play important roles in various cellular processes, both in physiological and in pathological conditions. Dimerization and autophosphorylation of these receptor tyrosine kinases are key events of signal transduction. Details of the molecular events of the signalin...

journal_title：Biophysical journal

authors： Bagossi P,Horváth G,Vereb G,Szöllösi J,Tözsér J

更新日期：2005-02-01 00:00:00

abstract：:Adsorption-desorption isotherms were obtained for water binding by 1,2-dimyristoylphosphatidylcholine in the temperature range 15 degrees-35 degrees C. The isotherms were analyzed by Brunauer et al.'s (BET) theory and also a polarization theory, the latter being more successful in fitting the data. There was some evid...

journal_title：Biophysical journal

authors： Wilkinson DA,Morowitz HJ,Prestegard JH

更新日期：1977-11-01 00:00:00

abstract：:We report here a multiprotein blind test of a computer method to predict native protein structures based solely on an all-atom physics-based force field. We use the AMBER 96 potential function with an implicit (GB/SA) model of solvation, combined with replica-exchange molecular-dynamics simulations. Coarse conformatio...

journal_title：Biophysical journal

authors： Shell MS,Ozkan SB,Voelz V,Wu GA,Dill KA

更新日期：2009-02-01 00:00:00

abstract：:Atomic force microscopy (AFM) is used to study mechanical properties of biological materials at submicron length scales. However, such samples are often structurally heterogeneous even at the local level, with different regions having distinct mechanical properties. Physical or chemical disruption can isolate individu...

journal_title：Biophysical journal

authors： Lee JJ,Rao S,Kaushik G,Azeloglu EU,Costa KD

更新日期：2018-06-05 00:00:00

abstract：:We show prolonged contraction of permeabilized muscle fibers of the frog during which structural order, as judged from low-angle x-ray diffraction, was preserved by means of partial cross-linking of the fibers using the zero-length cross-linker 1-ethyl-3-[3-dimethylamino)propyl]carbodiimide. Ten to twenty percent of t...

journal_title：Biophysical journal

authors： Bershitsky S,Tsaturyan A,Bershitskaya O,Mashanov G,Brown P,Webb M,Ferenczi MA

更新日期：1996-09-01 00:00:00

abstract：:Molecular diffusion process after the photo-induced electron injection to ferric cytochrome c (Fe(III) cyt c) in guanidine hydrochloride (GdnHCl) 3.5 M buffer solution is studied by the time-resolved transient grating technique. Circular dichroism studies have revealed that Fe(III) cyt c is unfolded under this conditi...

journal_title：Biophysical journal

authors： Nada T,Terazima M

更新日期：2003-09-01 00:00:00

abstract：:Moesin, a protein of the ezrin, radixin, and moesin family, which links the plasma membrane to the cytoskeleton, is involved in multiple physiological and pathological processes, including viral budding and infection. Its interaction with the plasma membrane occurs via a key phosphoinositide, the phosphatidyl(4,5)inos...

journal_title：Biophysical journal

authors： Lubart Q,Vitet H,Dalonneau F,Le Roy A,Kowalski M,Lourdin M,Ebel C,Weidenhaupt M,Picart C

更新日期：2018-01-09 00:00:00

abstract：:Sum-frequency vibrational spectroscopy (SFVS) is used to measure the intrinsic rate of lipid flip-flop for 1,2-dimyristoyl-sn-glycero-3-phosphocholine (DMPC), 1,2-dipalmitoyl-sn-glycero-3-phosphocholine (DPPC), and 1,2-distearoyl-sn-glycero-3-phosphocholine (DSPC) in planar-supported lipid bilayers (PSs). Asymmetric P...

journal_title：Biophysical journal

authors： Liu J,Conboy JC

更新日期：2005-10-01 00:00:00

abstract：:A computational analysis of d(GGGGTTTTGGGG)(2) guanine quadruplexes containing either lateral or diagonal four-thymidine loops was carried out using molecular dynamics (MD) simulations in explicit solvent, locally enhanced sampling (LES) simulations, systematic conformational search, and free energy molecular-mechanic...

journal_title：Biophysical journal

authors： Fadrná E,Spacková N,Stefl R,Koca J,Cheatham TE 3rd,Sponer J

更新日期：2004-07-01 00:00:00

abstract：:Enterococcus hirae V1-ATPase is a molecular motor composed of the A3B3 hexamer ring and the central stalk. In association with ATP hydrolysis, three catalytic AB pairs in the A3B3 ring undergo conformational changes, which lead to a 120° rotation of the central stalk. To understand how the conformational changes of th...

journal_title：Biophysical journal

authors： Isaka Y,Ekimoto T,Kokabu Y,Yamato I,Murata T,Ikeguchi M

更新日期：2017-03-14 00:00:00

abstract：:If a branched multistep editing mechanism is implemented by an enzyme with a single site for the specific substrates, there is no reason to believe that the number of testing steps is fixed and cannot be controlled by some external factors. The paper considers the mechanisms of single- or multistep editing done in res...

journal_title：Biophysical journal

authors： Kremen A

更新日期：1982-11-01 00:00:00

abstract：:We describe the use of asymmetric Ru-ligand complexes as a new class of luminescent probes that can be used to measure rotational motions of proteins. These complexes are known to display luminescent lifetimes ranging from 10 to 4000 ns. In this report, we show that the asymmetric complex Ru(bpy)2(dcbpy) (PF6)2 displa...

journal_title：Biophysical journal

authors： Terpetschnig E,Szmacinski H,Malak H,Lakowicz JR

更新日期：1995-01-01 00:00:00

abstract：:Using CD spectroscopy, guanine tetraplex formation was studied with short DNA fragments in which cytosine residues were systematically added to runs of guanine either at the 5' or 3' ends. Potassium cations induced the G-tetraplex more easily with fragments having the guanine run at the 5' end, which is just an opposi...

journal_title：Biophysical journal

authors： Penázová H,Vorlicková M

更新日期：1997-10-01 00:00:00

abstract：:The effect of transepithelial potential difference (psi) on Na and Cl flux across toad bladder was assessed by measuring isotopic flux between identical media at various values of psi. The contribution of edge damage to ionic permeability was eliminated, resulting in relatively high spontaneous psi (-97 +/-4 mv) and l...

journal_title：Biophysical journal

authors： Walser M

更新日期：1972-04-01 00:00:00