Membrane-wrapping contributions to malaria parasite invasion of the human erythrocyte.

abstract：:We demonstrate the first, to our knowledge, integration of stimulated emission depletion (STED) with selective plane illumination microscopy (SPIM). Using this method, we were able to obtain up to 60% improvements in axial resolution with lateral resolution enhancements in control samples and zebrafish embryos. The in...

journal_title：Biophysical journal

authors： Friedrich M,Gan Q,Ermolayev V,Harms GS

更新日期：2011-04-20 00:00:00

abstract：:Experimentally, short peptides have been shown to form amyloids similar to those of their parent proteins. Consequently, they present useful systems for studies of amyloid conformation. Here we simulate extensively the NFGAIL peptide, derived from the human islet amyloid polypeptide (residues 22-27). We simulate diffe...

journal_title：Biophysical journal

authors： Zanuy D,Ma B,Nussinov R

更新日期：2003-03-01 00:00:00

abstract：:The results of variable dielectric coefficient Poisson-Boltzmann calculations of the counter-ion concentration in the vicinity of an all-atom model of the B-form of DNA are presented with an emphasis on the importance of spatial variations in the dielectric properties of the solvent, particularly at the macro-ion-solv...

journal_title：Biophysical journal

authors： Pack GR,Garrett GA,Wong L,Lamm G

更新日期：1993-10-01 00:00:00

abstract：:DNA beacons are short single-stranded chains which can form closed hairpin shapes through complementary base pairing at their ends. Contrary to the common polymer theory assumption that only their loop length matters, experiments show that their closing kinetics depend on the loop composition. We have modeled the clos...

journal_title：Biophysical journal

authors： Aalberts DP,Parman JM,Goddard NL

更新日期：2003-05-01 00:00:00

abstract：:Cytosolic calcium oscillations occur in a wide variety of cells and are involved in different cellular functions. We describe these calcium oscillations by a mathematical model based on the putative electrophysiological properties of the endoplasmic reticulum (ER) membrane. The salient features of our membrane model a...

journal_title：Biophysical journal

authors： Jafri MS,Vajda S,Pasik P,Gillo B

更新日期：1992-07-01 00:00:00

abstract：:Cryo-electron microscopy is rapidly emerging as a powerful technique to determine the structures of complex macromolecular systems elusive to other techniques. Because many of these systems are highly dynamical, characterizing their movements is also a crucial step to unravel their biological functions. To achieve thi...

journal_title：Biophysical journal

authors： Bonomi M,Pellarin R,Vendruscolo M

更新日期：2018-04-10 00:00:00

abstract：:Cell proliferation and contact inhibition play a major role in maintaining epithelial cell homeostasis. Prior experiments have shown that externally applied forces, such as stretch, result in increased proliferation in an E-cadherin force-dependent manner. In this study, the spatial regulation of cell proliferation in...

journal_title：Biophysical journal

authors： Mohan A,Schlue KT,Kniffin AF,Mayer CR,Duke AA,Narayanan V,Arsenovic PT,Bathula K,Danielsson BE,Dumbali SP,Maruthamuthu V,Conway DE

更新日期：2018-09-04 00:00:00

abstract：:BP100 (KKLFKKILKYL-NH(2)) is a short cecropin A-melittin hybrid peptide, obtained through a combinatorial chemistry approach, which is highly effective in inhibiting both the in vitro and in vivo growth of economically important plant pathogenic Gram-negatives. The intrinsic Tyr fluorescence of BP100 was taken advanta...

journal_title：Biophysical journal

authors： Ferre R,Melo MN,Correia AD,Feliu L,Bardají E,Planas M,Castanho M

更新日期：2009-03-04 00:00:00

abstract：:The antifungal polyene antibiotic amphotericin B forms channels in lipid membranes that are permeable to ions, water, and nonelectrolytes. Anion, cation, and ion pair coordination in the water-filled pore of the "barrel" unit of the channels was studied by molecular dynamics simulations. Unlike the case of the gramici...

journal_title：Biophysical journal

authors： Khutorsky V

更新日期：1996-12-01 00:00:00

abstract：:A complete description of protein structure and function must include a proper treatment of mechanisms that lead to cooperativity. The helix/coil transition serves as a simple example of a cooperative folding process, commonly described by a nucleation-propagation mechanism. The prevalent view is that coil structure m...

journal_title：Biophysical journal

authors： Vorov OK,Livesay DR,Jacobs DJ

更新日期：2009-12-02 00:00:00

abstract：:Galactosylceramides (GalCers) containing nervonoyl (24:1(Delta15(cis))) acyl chains have the capacity to assemble into nanotubular microstructures in excess water (. Biophys. J. 69:1976-1986). To define the structural parameters that modulate nanotube formation, GalCer derivatives were synthesized that contained cis m...

journal_title：Biophysical journal

authors： Kulkarni VS,Boggs JM,Brown RE

更新日期：1999-07-01 00:00:00

abstract：:Limited proteolysis of RNase-A yields a short N-terminal S-peptide segment and the larger S-protein. Binding of S-peptide to S-protein results in the formation of an enzymatically active RNase-S protein. S-peptide undergoes a transition from intrinsic disorder to an ordered helical state upon association with S-protei...

journal_title：Biophysical journal

authors： Luitz MP,Bomblies R,Zacharias M

更新日期：2017-10-03 00:00:00

abstract：:The endoplasmic reticulum (ER) is the major compartment for the processing and quality control of newly synthesized proteins. Green fluorescent protein (GFP) was used as a noninvasive probe to determine the viscous properties of the aqueous lumen of the ER. GFP was targeted to the ER lumen of CHO cells by transient tr...

journal_title：Biophysical journal

authors： Dayel MJ,Hom EF,Verkman AS

更新日期：1999-05-01 00:00:00

abstract：:A phospholipid chelate complexed with ytterbium (DMPE-DTPA:Yb3+) is shown to be readily incorporated into a model membrane system, which may then be aligned in a magnetic field such that the average bilayer normal lies along the field. This so-called positively ordered smectic phase, whose lipids consist of less than ...

journal_title：Biophysical journal

authors： Prosser RS,Volkov VB,Shiyanovskaya IV

更新日期：1998-11-01 00:00:00

abstract：:The binding mechanism of sweet proteins to their receptor, a G-protein-coupled receptor, is not supported by direct structural information. In principle, the key groups responsible for biological activity (glucophores) can be localized on a small structural unit (sweet finger) or spread on a larger surface area. A rec...

journal_title：Biophysical journal

authors： De Simone A,Spadaccini R,Temussi PA,Fraternali F

更新日期：2006-05-01 00:00:00

abstract：:We study the folding mechanism of a triple beta-strand WW domain from the Formin binding protein 28 (FBP28) at atomic resolution with explicit water model using replica exchange molecular dynamics computer simulations. Extended sampling over a wide range of temperatures to obtain the free energy, enthalpy, and entropy...

journal_title：Biophysical journal

authors： Mu Y,Nordenskiöld L,Tam JP

更新日期：2006-06-01 00:00:00

abstract：:Human immunodeficiency virus-1 viral infectivity factor (Vif) is an intrinsically disordered protein responsible for the ubiquitination of the APOBEC3 (A3) antiviral proteins. Vif folds when it binds Cullin-RING E3 ligase 5 and the transcription cofactor CBF-β. A five-protein complex containing the substrate receptor ...

journal_title：Biophysical journal

authors： Ball KA,Chan LM,Stanley DJ,Tierney E,Thapa S,Ta HM,Burton L,Binning JM,Jacobson MP,Gross JD

更新日期：2019-04-23 00:00:00

abstract：:Stress fibers are cellular contractile actomyosin machines central to wound healing, shear stress response, and other processes. Contraction mechanisms have been difficult to establish because stress fibers in cultured cells typically exert isometric tension and present little kinetic activity. In a recent study, livi...

journal_title：Biophysical journal

authors： Stachowiak MR,O'Shaughnessy B

更新日期：2009-07-22 00:00:00

abstract：:Sedimentation velocity analytical ultracentrifugation is an important tool in the characterization of macromolecules and nanoparticles in solution. The sedimentation coefficient distribution c(s) of Lamm equation solutions is based on the approximation of a single, weight-average frictional coefficient of all particle...

journal_title：Biophysical journal

authors： Brown PH,Schuck P

更新日期：2006-06-15 00:00:00

abstract：:The antagonizing effect of high pressure against anesthesia is well known. With purified firefly luciferase, however,. Biophys. J. 60:1309-1314) reported that high pressure did not affect the initial flash intensity. Firefly luciferase emits a burst of light when the substrates luciferin and ATP are added in the prese...

journal_title：Biophysical journal

authors： Ueda I,Minami H,Matsuki H,Inoue T

更新日期：1999-01-01 00:00:00

abstract：:In addition to being the gateway for all access to the eukaryotic genome, chromatin has in recent years been identified as carrying an epigenetic code regulating transcriptional activity. Though much is known about the biochemistry of this code, little is understood regarding the different fiber structures through whi...

journal_title：Biophysical journal

authors： Depken M,Schiessel H

更新日期：2009-02-01 00:00:00

abstract：:The folding pathway of the small α/β protein GB1 has been extensively studied during the past two decades using both theoretical and experimental approaches. These studies provided a consensus view that the protein folds in a two-state manner. Here, we reassessed the folding of GB1, both by experiments and simulations...

journal_title：Biophysical journal

authors： Morrone A,Giri R,Toofanny RD,Travaglini-Allocatelli C,Brunori M,Daggett V,Gianni S

更新日期：2011-10-19 00:00:00

abstract：:The structure of polymer-decorated phospholipid monolayers at the solid-solution interface was investigated using neutron reflectometry. The monolayers were composed of distearoylphosphatidylethanolamine (DSPE) matrixed with varying amounts of DSPE-PEG (DSPE with polyethylene glycol covalently grafted to its headgroup...

journal_title：Biophysical journal

authors： Kuhl TL,Majewski J,Wong JY,Steinberg S,Leckband DE,Israelachvili JN,Smith GS

更新日期：1998-11-01 00:00:00

abstract：:Unlike most transmembrane proteins, phospholipids can migrate from one leaflet of the membrane to the other. Because this spontaneous lipid translocation (flip-flop) tends to be very slow, cells facilitate the process with enzymes that catalyze the transmembrane movement and thereby regulate the transbilayer lipid dis...

journal_title：Biophysical journal

authors： Doktorova M,Heberle FA,Marquardt D,Rusinova R,Sanford RL,Peyear TA,Katsaras J,Feigenson GW,Weinstein H,Andersen OS

更新日期：2019-03-05 00:00:00

abstract：:ATP-sensitive K(+)-channels link metabolism and excitability in neurons, myocytes, and pancreatic islets. Mutations in the pore-forming subunit (Kir6.2; KCNJ11) cause neonatal diabetes, developmental delay, and epilepsy by decreasing sensitivity to ATP inhibition and suppressing electrical activity. Mutations of resid...

journal_title：Biophysical journal

authors： Koster JC,Kurata HT,Enkvetchakul D,Nichols CG

更新日期：2008-11-15 00:00:00

abstract：:In this article we present a study of a new class of surfactants denoted as PEG1500-12-acyloxystearates, which have potential use as pharmaceutical solubilizers. These amphiphilic molecules present interesting properties with regard to cell damage effects. PEG1500-12-acyloxystearates with C(14) to C(16) acyloxy chains...

journal_title：Biophysical journal

authors： Vaccaro M,von Corswant C,Söderman O

更新日期：2007-12-15 00:00:00

abstract：:To understand better the effect of electrostatics on the rigidity of the DNA double helix, we define DNA*, the null isomer of DNA, as the hypothetical structure that would result from DNA if its phosphate groups were not ionized. For the purposes of theoretical analysis, we model DNA* as identical to ordinary DNA but ...

journal_title：Biophysical journal

authors： Manning GS

更新日期：2006-11-15 00:00:00

abstract：:Hint2, one of the five members of the superfamily of the histidine triad AMP-lysine hydrolase proteins, is expressed in mitochondria of various cell types. In human adrenocarcinoma cells, Hint2 modulates Ca(2+) handling by mitochondria. As Hint2 is highly expressed in hepatocytes, we investigated if this protein affec...

journal_title：Biophysical journal

authors： Ndiaye D,Collado-Hilly M,Martin J,Prigent S,Dufour JF,Combettes L,Dupont G

更新日期：2013-09-03 00:00:00

abstract：:Proteins must be stable to accomplish their biological function and to avoid enzymatic degradation. Constitutive proteolysis, however, is the main source of free amino acids used for de novo protein synthesis. In this paper the delicate balance of protein stability and degradability is discussed in the context of func...

journal_title：Biophysical journal

authors： Simon A,Dosztányi Zs,Rajnavölgyi E,Simon I

更新日期：2000-11-01 00:00:00

abstract：:Ca2+ entry under resting conditions may be important for contraction of vascular smooth muscle, but little is known about the mechanisms involved. Ca2+ leakage was studied in the A7r5 smooth muscle-derived cell line by patch-clamp techniques. Two channels that could mediate calcium influx at resting membrane potential...

journal_title：Biophysical journal

authors： Obejero-Paz CA,Jones SW,Scarpa A

更新日期：1998-09-01 00:00:00