Recombination dynamics in bacterial photosynthetic reaction centers.

abstract：:Intracellular Ca2+ was determined with the fura-2 technique during electrically induced contractions in the rabbit rectococcygeus smooth muscle at 22 degreesC. The muscles were electrically activated to give short, reproducible contractions. Intracellular [Ca2+] increased during activation; the increase in [Ca2+] prec...

journal_title：Biophysical journal

authors： Arner A,Malmqvist U,Rigler R

更新日期：1998-10-01 00:00:00

abstract：:It has been observed in numerical experiments that adding a cargo to a motor protein can regularize its gait. Here we explain these results via asymptotic analysis on a general stochastic motor protein model. This analysis permits a computation of various observables (e.g., the mean velocity) of the motor protein and ...

journal_title：Biophysical journal

authors： DeVille RE,Vanden-Eijnden E

更新日期：2008-09-15 00:00:00

abstract：:Nonconfluent fibroblasts are relatively depolarized when compared with confluent fibroblasts, and transient hyperpolarizations result from a range of external stimuli as well as internal cellular activities. This electrical activity ceases, along with growth and mitogenic activity, when the cells become confluent. A c...

journal_title：Biophysical journal

authors： Stockbridge LL,French AS

更新日期：1988-07-01 00:00:00

abstract：:The chemical shifts of specific (13)C and (15)N labels distributed throughout KIAGKIA-KIAGKIA-KIAGKIA (K3), an amphiphilic 21-residue antimicrobial peptide, prove that the peptide is in an all alpha-helical conformation in the bilayers of multilamellar vesicles (MLVs) containing dipalmitoylphosphatidylcholine and dipa...

journal_title：Biophysical journal

authors： Toke O,Maloy WL,Kim SJ,Blazyk J,Schaefer J

更新日期：2004-07-01 00:00:00

abstract：:The raft hypothesis proposes that microdomains enriched in sphingolipids, cholesterol, and specific proteins are transiently formed to accomplish important cellular tasks. Equivocally, detergent-resistant membranes were initially assumed to be identical to membrane rafts, because of similarities between their composit...

journal_title：Biophysical journal

authors： Casadei BR,Domingues CC,de Paula E,Riske KA

更新日期：2014-06-03 00:00:00

abstract：:The influence of electrostatic multipole moments up to hexadecapole on the dynamics of photodissociated carbon monoxide (CO) in myoglobin is investigated. The CO electrostatic potential is expressed as an expansion into atomic multipole moments of increasing order up to octopole which are obtained from a distributed m...

journal_title：Biophysical journal

authors： Plattner N,Meuwly M

更新日期：2008-04-01 00:00:00

abstract：:The two-microelectrode, voltage-clamp technique was applied to rabbit cardiac Purkinje fibers to study the interaction of tetrodotoxin (TTX) with the slowly inactivating Na current. Binding of TTX to rested, inactivated, and activated channels was estimated by measuring the relative decrease of current at the beginnin...

journal_title：Biophysical journal

authors： Carmeliet E

更新日期：1987-01-01 00:00:00

abstract：:The results of a 3-ns molecular dynamics simulation of the dodecamer duplex d(TATGGATCCATA)(2) recognized by the BamHI endonuclease are presented here. The DNA has been simulated as a flexible molecule using an AMBER force field and the Ewald summation method, which eliminates the undesired effects of truncation and p...

journal_title：Biophysical journal

authors： Castrignanò T,Chillemi G,Desideri A

更新日期：2000-09-01 00:00:00

abstract：:Protein-mediated DNA looping, such as that induced by the lactose repressor (LacI) of Escherichia coli, is a well-known gene regulation mechanism. Although researchers have given considerable attention to DNA looping by LacI, many unanswered questions about this mechanism, including the role of protein flexibility, re...

journal_title：Biophysical journal

authors： Hirsh AD,Lillian TD,Lionberger TA,Perkins NC

更新日期：2011-08-03 00:00:00

abstract：:Previous theoretical studies have shown that measuring the transverse current across DNA strands while they translocate through a nanopore or channel may provide a statistically distinguishable signature of the DNA bases, and may thus allow for rapid DNA sequencing. However, fluctuations of the environment, such as io...

journal_title：Biophysical journal

authors： Krems M,Zwolak M,Pershin YV,Di Ventra M

更新日期：2009-10-07 00:00:00

abstract：:Pore formation by membrane-active peptides, naturally encountered in innate immunity and infection, could have important medical and technological applications. Recently, the well-studied lytic peptide melittin has formed the basis for the development of combinatorial libraries from which potent pore-forming peptides ...

journal_title：Biophysical journal

authors： Sepehri A,PeBenito L,Pino-Angeles A,Lazaridis T

更新日期：2020-04-21 00:00:00

abstract：:To elucidate effects of electrostatic interactions resulting from surface charges on structures and phase stability of cubic phases of lipid membranes, membranes of 1-monoolein (MO) and dioleoylphosphatidic acid (DOPA) (DOPA/MO membrane) mixtures have been investigated by small-angle x-ray scattering method. As increa...

journal_title：Biophysical journal

authors： Li SJ,Yamashita Y,Yamazaki M

更新日期：2001-08-01 00:00:00

abstract：:The skeleton adapts to its mechanical usage, although at the cellular level, the distribution and magnitude of strains generated and their detection are ill-understood. The magnitude and nature of the strains to which cells respond were investigated using an atomic force microscope (AFM) as a microindentor. A confocal...

journal_title：Biophysical journal

authors： Charras GT,Horton MA

更新日期：2002-06-01 00:00:00

abstract：:ATP-sensitive K(+)-channels link metabolism and excitability in neurons, myocytes, and pancreatic islets. Mutations in the pore-forming subunit (Kir6.2; KCNJ11) cause neonatal diabetes, developmental delay, and epilepsy by decreasing sensitivity to ATP inhibition and suppressing electrical activity. Mutations of resid...

journal_title：Biophysical journal

authors： Koster JC,Kurata HT,Enkvetchakul D,Nichols CG

更新日期：2008-11-15 00:00:00

abstract：:The electrophoretic behavior of defined DNA and RNA oligonucleotide duplexes from 10 to 20 bp in length has been investigated as a function of salt conditions, gel concentration, and temperature. The RNA oligomers migrated much more slowly than the DNA oligomers of the same sequence under all conditions. From sediment...

journal_title：Biophysical journal

authors： Bonifacio GF,Brown T,Conn GL,Lane AN

更新日期：1997-09-01 00:00:00

abstract：:We present higher-order moment analysis of fluorescence intensity fluctuations from individual laser scanning microscopy images applied to study monomer-oligomer distributions. We demonstrate that the number densities and brightness ratios of a mixed population of monomers and oligomers can be determined by analyzing ...

journal_title：Biophysical journal

authors： Sergeev M,Costantino S,Wiseman PW

更新日期：2006-11-15 00:00:00

abstract：:Direct spectroscopic measurements of rotational motions of proteins and large protein segments are crucial to understanding the molecular dynamics of protein function. Fluorescent probes and spin labels attached to proteins have proved to be powerful tools in the study of large-scale protein motions. Fluorescence depo...

journal_title：Biophysical journal

authors： Thomas DD

更新日期：1978-11-01 00:00:00

abstract：:The influence of basic open-loop synaptic connections on the firing of simultaneously recorded neurons has been investigated with auto- and cross-correlation histograms, using experimental records and computer simulations. The basic connections examined were direct synaptic excitation, direct synaptic inhibition, and ...

journal_title：Biophysical journal

authors： Moore GP,Segundo JP,Perkel DH,Levitan H

更新日期：1970-09-01 00:00:00

abstract：:Values of K, delta G(o), delta H(o), delta S(o) and delta C(po) for the binding reaction of small organic ligands forming 1:1 complexes with either alpha- or beta-cyclodextrin were obtained by titration calorimetry from 15 degrees C to 45 degrees C. A hydrogen bond or hydrophobic interaction was introduced by adding a...

journal_title：Biophysical journal

authors： Ross PD,Rekharsky MV

更新日期：1996-10-01 00:00:00

abstract：:The bovine eye lens protein, alpha L crystallin, has been studied with photon correlation spectroscopy and statical light scattering in the concentration range up to 200 g/l in different solvent conditions. At higher concentration (c greater than 70 g/l) the scattering behavior is quite complicated, which results in n...

journal_title：Biophysical journal

authors： Andries C,Clauwaert J

更新日期：1985-05-01 00:00:00

abstract：:Synchrotron radiation circular dichroism, Fourier transform infrared, and nuclear magnetic resonance spectroscopies, and small-angle x-ray scattering were used to monitor the reversible thermal unfolding of hen egg white lysozyme. The results were compared with crystal structures and high- and low-temperature structur...

journal_title：Biophysical journal

authors： Meersman F,Atilgan C,Miles AJ,Bader R,Shang W,Matagne A,Wallace BA,Koch MH

更新日期：2010-10-06 00:00:00

abstract：:Chromatin organization within the nucleus is a vital regulator of genome function, yet its mechanical coupling to the nuclear architecture has remained elusive. To directly investigate this coupling, we locally modulated chromatin structure in living cells using nanoparticle-based laser perturbation. Unusual differenc...

journal_title：Biophysical journal

authors： Mazumder A,Shivashankar GV

更新日期：2007-09-15 00:00:00

abstract：:The conduction properties of ClC-0 and ClC-1 chloride channels are examined using electrostatic calculations and three-dimensional Brownian dynamics simulations. We create an open-state configuration of the prokaryotic ClC Cl(-) channel using its known crystallographic structure as a basis. Two residues that are occlu...

journal_title：Biophysical journal

authors： Corry B,O'Mara M,Chung SH

更新日期：2004-02-01 00:00:00

abstract：:We examined the kinetics of voltage-dependent sodium currents in cerebellar Purkinje neurons using whole-cell recording from dissociated neurons. Unlike sodium currents in other cells, recovery from inactivation in Purkinje neurons is accompanied by a sizeable ionic current. Additionally, the extent and speed of recov...

journal_title：Biophysical journal

authors： Raman IM,Bean BP

更新日期：2001-02-01 00:00:00

abstract：:RNA junctions are common secondary structural elements present in a wide range of RNA species. They play crucial roles in directing the overall folding of RNA molecules as well as in a variety of biological functions. In particular, there has been great interest in the dynamics of RNA junctions, including conformation...

journal_title：Biophysical journal

authors： Jung S,Schlick T

更新日期：2014-01-21 00:00:00

abstract：:The proline-, glutamate-, valine-, and lysine-rich (PEVK) domain of the giant muscle protein titin is thought to be an intrinsically unstructured random-coil segment. Various observations suggest, however, that the domain may not be completely devoid of internal interactions and structural features. To test the validi...

journal_title：Biophysical journal

authors： Huber T,Grama L,Hetényi C,Schay G,Fülöp L,Penke B,Kellermayer MS

更新日期：2012-10-03 00:00:00

abstract：:Photosystem II catalyzes photosynthetic water oxidation in plants, green algae, and cyanobacteria. The manganese-containing active site cycles through a series of five oxidation states, S(n), where n refers to the number of oxidizing equivalents stored. In this report, reaction-induced Fourier transform infrared and e...

journal_title：Biophysical journal

authors： Halverson KM,Barry BA

更新日期：2003-10-01 00:00:00

abstract：:The selective permeability to monovalent metal cations, as well as the relationship between cation permeation and gating kinetics, was investigated for native tetrodotoxin-insensitive Na-channels in guinea pig ventricular myocytes using the whole-cell patch clamp technique. By the measurement of inward unidirectional ...

journal_title：Biophysical journal

authors： Kurata Y,Sato R,Hisatome I,Imanishi S

更新日期：1999-10-01 00:00:00

abstract：:The structure and biophysical properties of lipid membranes are important for cellular functions in health and disease. In Alzheimer's disease, the neuronal membrane is a target for toxic amyloid-β (Aβ). Melatonin is an important pineal gland hormone that has been shown to protect against Aβ toxicity in cellular and a...

journal_title：Biophysical journal

authors： Mei N,Robinson M,Davis JH,Leonenko Z

更新日期：2020-12-15 00:00:00

abstract：:We report applications of two-photon excitation fluorescence (2PEF) microscopy with subdiffraction-limit resolution for green-fluorescent-protein-tagged cell imaging. The microscope integrates 2PEF microscopy and stimulated emission depletion microscopy in one microscope that has the benefits of both techniques: intri...

journal_title：Biophysical journal

authors： Li Q,Wu SS,Chou KC

更新日期：2009-12-16 00:00:00