Abstract:
:Biophysical understanding of membrane domains requires accurate knowledge of their structural details and elasticity. We report on a global small angle x-ray scattering data analysis technique for coexisting liquid-ordered (Lo) and liquid-disordered (Ld) domains in fully hydrated multilamellar vesicles. This enabled their detailed analysis for differences in membrane thickness, area per lipid, hydrocarbon chain length, and bending fluctuation as demonstrated for two ternary mixtures (DOPC/DSPC/CHOL and DOPC/DPPC/CHOL) at different cholesterol concentrations. Lo domains were found to be ~10 Å thicker, and laterally up to 20 Å(2)/lipid more condensed than Ld domains. Their bending fluctuations were also reduced by ~65%. Increase of cholesterol concentration caused significant changes in structural properties of Ld, while its influence on Lo properties was marginal. We further observed that temperature-induced melting of Lo domains is associated with a diffusion of cholesterol to Ld domains and controlled by Lo/Ld thickness differences.
journal_name
Biophys Jjournal_title
Biophysical journalauthors
Heftberger P,Kollmitzer B,Rieder AA,Amenitsch H,Pabst Gdoi
10.1016/j.bpj.2014.11.3488subject
Has Abstractpub_date
2015-02-17 00:00:00pages
854-862issue
4eissn
0006-3495issn
1542-0086pii
S0006-3495(14)04817-6journal_volume
108pub_type
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