Abstract:
:Recently various pathways of human telomere (ht) DNA folding into G-quadruplexes and of ligand binding to these structures have been proposed. However, the key issue as to the nature of forces driving the folding and recognition processes remains unanswered. In this study, structural changes of 22-mer ht-DNA fragment (Tel22), induced by binding of ions (K(+), Na(+)) and specific bisquinolinium ligands, were monitored by calorimetric and spectroscopic methods and by gel electrophoresis. Using the global model analysis of a wide variety of experimental data, we were able to characterize the thermodynamic forces that govern the formation of stable Tel22 G-quadruplexes, folding intermediates, and ligand-quadruplex complexes, and then predict Tel22 behavior in aqueous solutions as a function of temperature, salt concentration, and ligand concentration. On the basis of the above, we believe that our work sets the framework for better understanding the heterogeneity of ht-DNA folding and binding pathways, and its structural polymorphism.
journal_name
Biophys Jjournal_title
Biophysical journalauthors
Bončina M,Hamon F,Islam B,Teulade-Fichou MP,Vesnaver G,Haider S,Lah Jdoi
10.1016/j.bpj.2015.05.001subject
Has Abstractpub_date
2015-06-16 00:00:00pages
2903-11issue
12eissn
0006-3495issn
1542-0086pii
S0006-3495(15)00458-0journal_volume
108pub_type
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