Kinetic constants of the acetylcholine (ACh) receptor reaction deduced from the rise in open probability after steps in ACh concentration.

abstract：:Tension and dynamic stiffness of passive rabbit psoas, rabbit semitendinosus, and waterbug indirect flight muscles were investigated to study the contribution of weak-binding cross-bridges and elastic filaments (titin and minititin) to the passive mechanical behavior of these muscles. Experimentally, a functional diss...

journal_title：Biophysical journal

authors： Granzier HL,Wang K

更新日期：1993-11-01 00:00:00

abstract：:Herein, we identify the coordination environment of Cu²(+) in the human α1-glycine receptor (GlyR). GlyRs are members of the pentameric ligand-gated ion channel superfamily (pLGIC) that mediate fast signaling at synapses. Metal ions like Zn²(+) and Cu²(+) significantly modulate the activity of pLGICs, and metal ion co...

journal_title：Biophysical journal

authors： Ruthstein S,Stone KM,Cunningham TF,Ji M,Cascio M,Saxena S

更新日期：2010-10-20 00:00:00

abstract：:Phototropins, major blue-light receptors in plants, are sensitive to blue light through a pair of flavin mononucleotide (FMN)-binding light oxygen and voltage (LOV) domains, LOV1 and LOV2. LOV2 undergoes a photocycle involving light-driven covalent adduct formation between a conserved cysteine and the FMN C(4a) atom. ...

journal_title：Biophysical journal

authors： Alexandre MT,Domratcheva T,Bonetti C,van Wilderen LJ,van Grondelle R,Groot ML,Hellingwerf KJ,Kennis JT

更新日期：2009-07-08 00:00:00

abstract：:The axial deformation of a pipette-pressurized fluid membrane bag produces minuscule yet well-defined, reproducible forces. The stiffness of this ultrasensitive force transducer is tunable and largely independent of the constitutive membrane behavior. Based on a rigorous variational treatment, we present both numerica...

journal_title：Biophysical journal

authors： Heinrich V,Ounkomol C

更新日期：2007-07-15 00:00:00

abstract：:Imaging in any plane other than horizontal in a microscope typically requires a reconstruction from multiple optical slices that significantly decreases the spatial and temporal resolution that can be achieved. This can limit the precision with which molecular events can be detected, for example, at intercellular cont...

journal_title：Biophysical journal

authors： Oddos S,Dunsby C,Purbhoo MA,Chauveau A,Owen DM,Neil MA,Davis DM,French PM

更新日期：2008-11-15 00:00:00

abstract：:The electrophysiological properties of the cation channel of the purified nicotinic acetylcholine receptor (AChR) reconstituted in planar lipid bilayers were characterized. Single-channel currents were activated by acetylcholine, carbamylcholine and suberyldicholine. The single channel conductance (28 pS in 0.3 M NaCl...

journal_title：Biophysical journal

authors： Montal M,Labarca P,Fredkin DR,Suarez-Isla BA

更新日期：1984-01-01 00:00:00

abstract：:Using a model for catalysis of a dynamic equilibrium, the role of constraint in catalysis is quantified. The intrinsic rigidity of proteins is shown to be insufficient to constrain the activated complexes of enzymes, irrespective of the mechanism. However, when minimization of the surface excess free energy of water s...

journal_title：Biophysical journal

authors： Vanselow DG

更新日期：2002-05-01 00:00:00

abstract：:The structural basis of the wide variability of the physical properties of fibrin clots and the process of assembly of the clot were investigated by electron microscopy of fibers formed under various ionic conditions. In addition, highly specific proteolytic enzymes from different snake venoms were used to remove sele...

journal_title：Biophysical journal

authors： Weisel JW

更新日期：1986-12-01 00:00:00

abstract：:The influence of basic open-loop synaptic connections on the firing of simultaneously recorded neurons has been investigated with auto- and cross-correlation histograms, using experimental records and computer simulations. The basic connections examined were direct synaptic excitation, direct synaptic inhibition, and ...

journal_title：Biophysical journal

authors： Moore GP,Segundo JP,Perkel DH,Levitan H

更新日期：1970-09-01 00:00:00

abstract：:The development of strategies to measure plasma membrane osmotic water permeability (Pf) in epithelial cells has been motivated by the identification of a family of molecular water channels. A general approach utilizing interferometry to measure cell shape and volume was developed and applied to measure Pf in cell lay...

journal_title：Biophysical journal

authors： Farinas J,Verkman AS

更新日期：1996-12-01 00:00:00

abstract：:Various differential polarization images or Mueller images of model objects are generated using the equations derived in the previous paper (paper I of this series). These calculated images include models of the higher-order organization of metaphase chromosomes, and show the applicability of the differential polariza...

journal_title：Biophysical journal

authors： Kim M,Ulibarri L,Bustamante C

更新日期：1987-12-01 00:00:00

abstract：:Intracellular recordings of cortical neurons in vivo display intense subthreshold membrane potential (V(m)) activity. The power spectral density of the V(m) displays a power-law structure at high frequencies (>50 Hz) with a slope of approximately -2.5. This type of frequency scaling cannot be accounted for by traditio...

journal_title：Biophysical journal

authors： Bédard C,Destexhe A

更新日期：2008-02-15 00:00:00

abstract：:The influence of patch electrode glass composition on macroscopic IV relations in inside-out patches of the cGMP-activated ion channel from rod photoreceptors was examined for a soda lime glass, a Kovar sealing glass, a borosilicate glass, and several soft lead glasses. In several glasses the shape or magnitude of the...

journal_title：Biophysical journal

authors： Furman RE,Tanaka JC

更新日期：1988-02-01 00:00:00

abstract：:A system based on two designed peptides, namely the cationic peptide K, (KIAALKE)3, and its complementary anionic counterpart called peptide E, (EIAALEK)3, has been used as a minimal model for membrane fusion, inspired by SNARE proteins. Although the fact that docking of separate vesicle populations via the formation ...

journal_title：Biophysical journal

authors： Rabe M,Aisenbrey C,Pluhackova K,de Wert V,Boyle AL,Bruggeman DF,Kirsch SA,Böckmann RA,Kros A,Raap J,Bechinger B

更新日期：2016-11-15 00:00:00

abstract：:The yolk granule is the most abundant membrane-bound organelle present in sea urchin eggs and embryos. The major protein component of this organelle, toposome, accounts for approximately 50% of the total yolk protein and has been shown to be localized to the embryonic cell surface. Extensive characterization in severa...

journal_title：Biophysical journal

authors： Hayley M,Emberley J,Davis PJ,Morrow MR,Robinson JJ

更新日期：2006-12-15 00:00:00

abstract：:We have previously observed that the hydrophobic polyelectrolyte poly(2-ethylacrylic acid) solubilizes lipid membranes in a pH-dependent manner, and we have exploited this phenomenon to prepare lipid vesicles that release their contents in response to pH, light, or glucose (Thomas, J. L., and D. A. Tirrell. Acc. Chem....

journal_title：Biophysical journal

authors： Thomas JL,Barton SW,Tirrell DA

更新日期：1994-09-01 00:00:00

abstract：:The E5 oncoprotein is the major transforming protein of bovine papillomavirus type 1. This 44-residue transmembrane protein can interact with the platelet-derived growth factor receptor β, leading to ligand-independent activation and cell transformation. For productive interaction, E5 needs to dimerize via a C-termina...

journal_title：Biophysical journal

authors： Windisch D,Hoffmann S,Afonin S,Vollmer S,Benamira S,Langer B,Bürck J,Muhle-Goll C,Ulrich AS

更新日期：2010-09-22 00:00:00

abstract：:Cubic insulin crystals contain a binding site for monovalent cations in a cavity of the crystal dyad in which the bound cation is ligated by protein atomic dipoles and water molecules. These types of interaction are analogous to interactions that occur in small cation-selective carrier and channel molecules. X-ray dif...

journal_title：Biophysical journal

authors： Badger J,Kapulsky A,Gursky O,Bhyravbhatla B,Caspar DL

更新日期：1994-02-01 00:00:00

abstract：:Calmodulin (CaM) is an intracellular cooperative calcium-binding protein essential for activating many diverse target proteins. Biophysical studies of the calcium-induced conformational changes of CaM disagree on the structure of the linker between domains and possible orientations of the domains. Molecular dynamics s...

journal_title：Biophysical journal

authors： Sorensen BR,Shea MA

更新日期：1996-12-01 00:00:00

abstract：:Small amounts of paramagnetic cations quench the phosphorescence of DNA. Although the emission intensity is monotonic with increasing thymine content, the quenching efficiency is not. The cations quench from phosphate sites. ...

journal_title：Biophysical journal

authors： Isenberg I,Rosenbluth R,Baird SL Jr

更新日期：1967-07-01 00:00:00

abstract：:Ubiquinol cytochrome c oxidoreductase (bc1 complex) serves as an important electron junction in many respiratory systems. It funnels electrons coming from NADH and ubiquinol to cytochrome c, but it is also capable of producing significant amounts of the free radical superoxide. In situ and in other experimental system...

journal_title：Biophysical journal

authors： Bazil JN

更新日期：2017-10-03 00:00:00

abstract：:There is increasing evidence that UVA radiation, which makes up approximately 95% of the solar UV light reaching the Earth's surface and is also commonly used for cosmetic purposes, is genotoxic. However, in contrast to UVC and UVB, the mechanisms by which UVA produces various DNA lesions are still unclear. In additio...

journal_title：Biophysical journal

authors： Jiang Y,Rabbi M,Kim M,Ke C,Lee W,Clark RL,Mieczkowski PA,Marszalek PE

更新日期：2009-02-01 00:00:00

abstract：:Motions through the energy landscape of proteins lead to biological function. At temperatures below a dynamical transition (150-250 K), some of these motions are arrested and the activity of some proteins ceases. Here, we introduce the technique of temperature-derivative fluorescence microspectrophotometry to investig...

journal_title：Biophysical journal

authors： Weik M,Vernede X,Royant A,Bourgeois D

更新日期：2004-05-01 00:00:00

abstract：:The influence of electrostatic multipole moments up to hexadecapole on the dynamics of photodissociated carbon monoxide (CO) in myoglobin is investigated. The CO electrostatic potential is expressed as an expansion into atomic multipole moments of increasing order up to octopole which are obtained from a distributed m...

journal_title：Biophysical journal

authors： Plattner N,Meuwly M

更新日期：2008-04-01 00:00:00

abstract：:A microscopic model of passive transverse mass transport of small solutes in the viable epidermal layer of human skin is formulated on the basis of a hexagonal array of cells (i.e., keratinocytes) bounded by 4-nm-thick, anisotropic lipid bilayers and separated by 1-μm layers of extracellular fluid. Gap junctions and t...

journal_title：Biophysical journal

authors： Nitsche JM,Kasting GB

更新日期：2013-05-21 00:00:00

abstract：:Reverberating spontaneous synchronized brain activity is believed to play an important role in neural information processing. Whether and how external stimuli can influence this spontaneous activity is poorly understood. Because periodic synchronized network activity is also prominent in in vitro neuronal cultures, we...

journal_title：Biophysical journal

authors： Vajda I,van Pelt J,Wolters P,Chiappalone M,Martinoia S,van Someren E,van Ooyen A

更新日期：2008-06-01 00:00:00

abstract：:A 10-ns molecular dynamics simulation of mouse acetylcholinesterase was analyzed, with special attention paid to the fluctuation in the width of the gorge and opening events of the back door. The trajectory was first verified to ensure its stability. We defined the gorge proper radius as the measure for the extent of ...

journal_title：Biophysical journal

authors： Tai K,Shen T,Börjesson U,Philippopoulos M,McCammon JA

更新日期：2001-08-01 00:00:00

abstract：:The wide use of lattice-sum strategies in biomolecular simulations has raised many questions on potential artifacts in these strategies. One interesting question is the artifacts in the counterion distributions of highly charged systems. As one would anticipate, Coulombic interactions under the periodic boundary condi...

journal_title：Biophysical journal

authors： Ye X,Cai Q,Yang W,Luo R

更新日期：2009-07-22 00:00:00

abstract：:A 3D model was developed and used to explore dynamics of phosphatidylinositol-4,5-bisphosphate (PIP2) signaling in cerebellar Purkinje neurons. Long-term depression in Purkinje neurons depends on coincidence detection of climbing fiber stimulus evoking extracellular calcium flux into the cell and parallel fiber stimul...

journal_title：Biophysical journal

authors： Brown SA,Morgan F,Watras J,Loew LM

更新日期：2008-08-01 00:00:00

abstract：:It has been a long belief that release of substances from the cell to the extracellular milieu by exocytosis is completed by diffusion of the substances from secretory vesicles through the fusion pore. Involvement of any mechanical force that may be superposed on the diffusion to enhance the releasing process has not ...

journal_title：Biophysical journal

authors： Tsuboi T,Kikuta T,Sakurai T,Terakawa S

更新日期：2002-07-01 00:00:00