Abstract:
:We have studied the atomic level structure of mineralized articular cartilage with heteronuclear solid-state NMR, our aims being to identify the inorganic species present at the surfaces of the mineral crystals which may interact with the surrounding organic matrix and to determine which components of the organic matrix are most closely involved with the mineral crystals. One-dimensional (1)H and (31)P and two-dimensional (1)H-(31)P heteronuclear correlation NMR experiments show that the mineral component is very similar to that in bone with regard to its surface structure. (13)C{(31)P} rotational echo double resonance experiments identify the organic molecules at the mineral surface as glycosaminoglycans, which concurs with our recent finding in bone. There is also evidence of gamma-carboxyglutamic acid residues interacting with the mineral. However, other matrix components appear more distant from the mineral compared with bone. This may be due to a larger hydration layer on the mineral crystal surfaces in calcified cartilage.
journal_name
Biophys Jjournal_title
Biophysical journalauthors
Duer MJ,Friscić T,Murray RC,Reid DG,Wise ERdoi
10.1016/j.bpj.2008.12.3954subject
Has Abstractpub_date
2009-04-22 00:00:00pages
3372-8issue
8eissn
0006-3495issn
1542-0086pii
S0006-3495(09)00482-2journal_volume
96pub_type
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