Abstract:
:We derive the statistics of the signals generated by shape fluctuations of large molecules studied by feedback tracking microscopy. We account for the influence of intramolecular dynamics on the response of the tracking system and derive a general expression for the fluorescence autocorrelation function that applies when those dynamics are linear. We show that in comparison to traditional fluorescence correlation spectroscopy, tracking provides enhanced sensitivity to translational diffusion, molecular size, heterogeneity, and long-timescale decays. We demonstrate our approach using a three-dimensional tracking microscope to study genomic lambda-phage DNA molecules with various fluorescence label configurations.
journal_name
Biophys Jjournal_title
Biophysical journalauthors
McHale K,Mabuchi Hdoi
10.1016/j.bpj.2010.03.045subject
Has Abstractpub_date
2010-07-07 00:00:00pages
313-22issue
1eissn
0006-3495issn
1542-0086pii
S0006-3495(10)00410-8journal_volume
99pub_type
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journal_title:Biophysical journal
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journal_title:Biophysical journal
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journal_title:Biophysical journal
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journal_title:Biophysical journal
pub_type: 杂志文章
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pub_type: 杂志文章
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