Intramolecular fluorescence correlation spectroscopy in a feedback tracking microscope.

Abstract:

:We derive the statistics of the signals generated by shape fluctuations of large molecules studied by feedback tracking microscopy. We account for the influence of intramolecular dynamics on the response of the tracking system and derive a general expression for the fluorescence autocorrelation function that applies when those dynamics are linear. We show that in comparison to traditional fluorescence correlation spectroscopy, tracking provides enhanced sensitivity to translational diffusion, molecular size, heterogeneity, and long-timescale decays. We demonstrate our approach using a three-dimensional tracking microscope to study genomic lambda-phage DNA molecules with various fluorescence label configurations.

journal_name

Biophys J

journal_title

Biophysical journal

authors

McHale K,Mabuchi H

doi

10.1016/j.bpj.2010.03.045

subject

Has Abstract

pub_date

2010-07-07 00:00:00

pages

313-22

issue

1

eissn

0006-3495

issn

1542-0086

pii

S0006-3495(10)00410-8

journal_volume

99

pub_type

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