Exploration of Insulin Amyloid Polymorphism Using Raman Spectroscopy and Imaging.

abstract：:The detection of the 1H NMR signal of myoglobin (Mb) in tissue opens an opportunity to examine its cellular diffusion property, which is central to its purported role in facilitating oxygen transport. In perfused myocardium the field-dependent transverse relaxation analysis of the deoxy Mb proximal histidyl NdeltaH in...

journal_title：Biophysical journal

authors： Wang D,Kreutzer U,Chung Y,Jue T

更新日期：1997-11-01 00:00:00

abstract：:We investigate the permeability of lipid membranes for fluorescence dyes and ions. We find that permeability reaches a maximum close to the chain melting transition of the membranes. Close to transitions, fluctuations in area and compressibility are high, leading to an increased likelihood of spontaneous lipid pore fo...

journal_title：Biophysical journal

authors： Blicher A,Wodzinska K,Fidorra M,Winterhalter M,Heimburg T

更新日期：2009-06-03 00:00:00

abstract：:Singlet-triplet mixing in the initial radical-pair state, P[unk]I[unk], of photosynthetic bacterial reaction centers is due to the hyperfine mechanism at low magnetic fields and both the hyperfine and Deltag mechanisms at high magnetic fields (>1 kG). Since the hyperfine field felt by the electron spins in P[unk]I[unk...

journal_title：Biophysical journal

authors： Goldstein RA,Boxer SG

更新日期：1987-06-01 00:00:00

abstract：:Phosphorus-31 nuclear magnetic resonance (31P-NMR) spectra were obtained from living toad retinae and toad retinal extracts at 4 degrees C. Several phosphorus metabolites--nucleoside di- and triphosphates (NTP), phosphocreatine, phosphodiesters, inorganic phosphate, and phosphomonoesters--were identified from the spec...

journal_title：Biophysical journal

authors： Apte DV,Koutalos Y,McFarlane DK,Dawson MJ,Ebrey TG

更新日期：1989-09-01 00:00:00

abstract：:Dispersed pump-dump-probe spectroscopy has the ability to characterize and identify the underlying ultrafast dynamical processes in complicated chemical and biological systems. This technique builds on traditional pump-probe techniques by exploring both ground- and excited-state dynamics and characterizing the connect...

journal_title：Biophysical journal

authors： Larsen DS,Vengris M,van Stokkum IH,van der Horst MA,de Weerd FL,Hellingwerf KJ,van Grondelle R

更新日期：2004-04-01 00:00:00

abstract：:We investigated the accessibility to protons and thiol-directed reagents of a cysteine substituted at position 338 in transmembrane segment 6 (TM6) of CFTR to test the hypothesis that T338 resides in the pore. Xenopus oocytes expressing T338C CFTR exhibited pH-dependent changes in gCl and I-V shape that were specific ...

journal_title：Biophysical journal

authors： Liu X,Zhang ZR,Fuller MD,Billingsley J,McCarty NA,Dawson DC

更新日期：2004-12-01 00:00:00

abstract：:A detailed picture of water and ion properties in small pores is important for understanding the behavior of biological ion channels. Several recent modeling studies have shown that small, hydrophobic pores exclude water and ions even if they are physically large enough to accommodate them, a mechanism called hydropho...

journal_title：Biophysical journal

authors： Spronk SA,Elmore DE,Dougherty DA

更新日期：2006-05-15 00:00:00

abstract：:We used high-resolution atomic force microscopy to image the compaction of linear and circular DNA by the yeast mitochondrial protein Abf2p, which plays a major role in packaging mitochondrial DNA. Atomic force microscopy images show that protein binding induces drastic bends in the DNA backbone for both linear and ci...

journal_title：Biophysical journal

authors： Friddle RW,Klare JE,Martin SS,Corzett M,Balhorn R,Baldwin EP,Baskin RJ,Noy A

更新日期：2004-03-01 00:00:00

abstract：:Interactions between proteins are often sufficiently weak that their study through the use of conventional structural techniques becomes problematic. Of the few techniques capable of providing experimental measures of weak protein-protein interactions, perhaps the most useful is the second virial coefficient, B(22), w...

journal_title：Biophysical journal

authors： Elcock AH,McCammon JA

更新日期：2001-02-01 00:00:00

abstract：:The protonation state and intracellular distribution of ellipticine were investigated in single human mammary T47D cells by confocal laser microspectrofluorimetry. In the cell nucleus, only the protonated form of ellipticine was detected as a direct consequence of its apparent pK increase upon DNA binding. Both proton...

journal_title：Biophysical journal

authors： Sureau F,Moreau F,Millot JM,Manfait M,Allard B,Aubard J,Schwaller MA

更新日期：1993-11-01 00:00:00

abstract：:Microstructured surfaces provide a unique framework to probe cell migration and cytoskeletal dynamics in a standardized manner. Here, we report on the steady-state occupancy probability of cells in asymmetric two-state microstructures that consist of two fibronectin-coated adhesion sites connected by a thin guidance c...

journal_title：Biophysical journal

authors： Fink A,Brückner DB,Schreiber C,Röttgermann PJF,Broedersz CP,Rädler JO

更新日期：2020-02-04 00:00:00

abstract：:We study the effect of ergosterol on the physical properties of 1-[(2)H(31)]palmitoyl-2-oleoyl-sn-glycero-3-phosphoethanolamine (POPE) multibilayers using deuterium nuclear magnetic resonance. NMR spectra were taken as a function of temperature and ergosterol concentration up to 70 mol %. The spectral first moments sh...

journal_title：Biophysical journal

authors： Hsueh YW,Weng CJ,Chen MT,Thewalt J,Zuckermann M

更新日期：2010-04-07 00:00:00

abstract：:Titin is a very large (>3 MDa) protein found in striated muscle where it is believed to participate in myogenesis and passive tension. A prominent feature in the A-band portion of titin is the presence of an 11-domain super-repeat of immunoglobulin superfamily and fibronectin-type-III-like domains. Seven overlapping c...

journal_title：Biophysical journal

authors： Head JG,Houmeida A,Knight PJ,Clarke AR,Trinick J,Brady RL

更新日期：2001-09-01 00:00:00

abstract：:Xenopus oocytes are widely employed for heterologous expression of cloned proteins, particularly electrogenic molecules such as ion channels and transporters. The high levels of expression readily obtained permit detailed investigations without interference from endogenous conductances. Injection of min K mRNA into Xe...

journal_title：Biophysical journal

authors： Tzounopoulos T,Maylie J,Adelman JP

更新日期：1995-09-01 00:00:00

abstract：:Intracellular reactions are carried out in a crowded medium where the macromolecules occupy ∼40% of the total volume. This decrease in the available volume affects the activity of the reactants. Scaled particle theory is used for the estimation of the activity coefficients of the metabolites, and thereby for the asses...

journal_title：Biophysical journal

authors： Angeles-Martinez L,Theodoropoulos C

更新日期：2015-12-01 00:00:00

abstract：:With their longest dimension typically being less than 100 nm, molecular motors are significantly below the optical-resolution limit. Despite substantial advances in fluorescence-based imaging methodologies, labeling with beads remains critical for optical-trapping-based investigations of molecular motors. A key exper...

journal_title：Biophysical journal

authors： Li Q,King SJ,Gopinathan A,Xu J

更新日期：2016-06-21 00:00:00

abstract：:Time courses of myelin lattice swelling in toad sciatic nerves preexposed to different treatments were determined by x-ray diffraction using a one-dimensional position-sensitive detector. In the nerves supramaximally stimulated for 1 h at 200 Hz, the subsequent process of myelin swelling occurred 45.0 +/- 7.3 min (n =...

journal_title：Biophysical journal

authors： Padrón R,Mateu L

更新日期：1982-08-01 00:00:00

abstract：:Rearrangement of tertiary structure in response to mechanical force (termed tertiary structure elasticity) in the tandem Ig chain is the first mode of elastic response for muscle protein titin. Tertiary structure elasticity occurs at low stretching forces (few tens of pN), and was described at atomic resolution in a r...

journal_title：Biophysical journal

authors： Hsin J,Schulten K

更新日期：2011-02-16 00:00:00

abstract：:Magic-angle spinning nuclear magnetic resonance is well suited for the study of membrane proteins in the nativelike lipid environment. However, the natural cellular membrane is invariably more complex than the proteoliposomes most often used for solid-state NMR (SSNMR) studies, and differences may affect the structure...

journal_title：Biophysical journal

authors： Ward ME,Wang S,Munro R,Ritz E,Hung I,Gor'kov PL,Jiang Y,Liang H,Brown LS,Ladizhansky V

更新日期：2015-04-07 00:00:00

abstract：:It has long been recognized that protein dynamical processes occur over a wide temporal range. However, the functionality of this spectrum of events remains unclear. In this work, a generalized noise function analysis is applied to a collection of diverse protein dynamical systems. It is shown that a power law model w...

journal_title：Biophysical journal

authors： Dewey TG,Bann JG

更新日期：1992-08-01 00:00:00

abstract：:Many complex cellular processes from mitosis to cell motility depend on the ability of the cytoskeleton to generate force. Force-generating systems that act on elastic cytoskeletal elements are prone to oscillating instabilities. In this work, we have measured spontaneous shape and movement oscillations in motile fish...

journal_title：Biophysical journal

authors： Barnhart EL,Allen GM,Jülicher F,Theriot JA

更新日期：2010-03-17 00:00:00

abstract：:Nonnative protein aggregation, which is a common feature in biotechnology, is also a clinical feature in more than 20 serious degenerative diseases. We studied the specific events of bovine pancreatic ribonuclease A thermal aggregation by a combination of second derivative infrared analysis and two-dimensional infrare...

journal_title：Biophysical journal

authors： Yan YB,Zhang J,He HW,Zhou HM

更新日期：2006-04-01 00:00:00

abstract：:Adaptation of the attractant response in Escherichia coli is attributable to the methylation of its transmembrane chemotactic receptors by the methyltransferase CheR. This protein contains two binding domains, one for the sites of methylation themselves and the other for a flexible tether at the C terminus of the rece...

journal_title：Biophysical journal

authors： Levin MD,Shimizu TS,Bray D

更新日期：2002-04-01 00:00:00

abstract：:The effect of weak, nonspecific interaction between molecules confined within restricted elements of volume ("pores") and the boundary surfaces of the pore, upon the reactivity and physical state of the confined molecules, is explored by means of simple models. A confined molecule is represented by a rectangular paral...

journal_title：Biophysical journal

authors： Minton AP

更新日期：1995-04-01 00:00:00

abstract：:A newly developed microscope using acousto-optic tunable filters (AOTFs) was used to generate in vivo hemoglobin saturation (SO2) and oxygen tension (PO2) maps in the cerebral cortex of mice. SO2 maps were generated from the spectral analysis of reflected absorbance images collected at different wavelengths, and PO2 m...

journal_title：Biophysical journal

authors： Shonat RD,Wachman ES,Niu W,Koretsky AP,Farkas DL

更新日期：1997-09-01 00:00:00

abstract：:Mycolactone, a cytotoxic and immunosuppressive macrolide produced by Mycobacterium ulcerans, is the central virulent factor in the skin disease Buruli ulcer. This multifunctional cytotoxin affects fundamental cellular processes such as cell adhesion, immune response, and cell death by targeting various cellular struct...

journal_title：Biophysical journal

authors： Aydin F,Sun R,Swanson JMJ

更新日期：2019-07-09 00:00:00

abstract：:The average sizes of fluid and gel domains in the two-component, two-phase system formed from mixtures of dimyristoyl phosphatidylcholine and distearoyl phosphatidylcholine were determined from an analysis of the electron spin resonance spectral lineshapes of a dimyristoyl phosphatidylcholine-nitroxide spin label as a...

journal_title：Biophysical journal

authors： Sankaram MB,Marsh D,Thompson TE

更新日期：1992-08-01 00:00:00

abstract：:The most probable source of force generation in muscle fibers in the rotation of the myosin head when bound to actin. This laboratory has demonstrated that ATP induces microsecond rotational motions of spin-labeled myosin heads bound to actin (Berger, C. L. E. C. Svensson, and D. D. Thomas. 1989. Proc. Natl. Acad. Sci...

journal_title：Biophysical journal

authors： Ostap EM,Thomas DD

更新日期：1991-06-01 00:00:00

abstract：:Efficient and rapid immune response upon challenge by an infectious agent is vital to host defense. The encounter of leukocytes (white blood cells of the immune system) with their targets is the first step in this response. Analysis of the kinetics of this process is essential not only to understanding dynamic behavio...

journal_title：Biophysical journal

authors： Charnick SB,Lauffenburger DA

更新日期：1990-05-01 00:00:00

abstract：:Conductance noise measurement of the open states of alamethicin transmembrane channels reveals excess noise attributable to cooperative low-frequency molecular dynamics that can generate fluctuations approximately 1 A rms in the effective channel pore radius. Single-channel currents through both persistent and nonpers...

journal_title：Biophysical journal

authors： Mak DO,Webb WW

更新日期：1995-12-01 00:00:00