DNA diffusion in mucus: effect of size, topology of DNAs, and transfection reagents.

abstract：:The general formalism required to treat two-state sliding filament models of muscle contraction, including free energy considerations, is first reviewed and amplified. This formalism is then used to examine, and modify as needed, three models studied previously by Podolsky and Nolan, in which cross-bridge attachment-d...

journal_title：Biophysical journal

authors： Hill TL,Eisenberg E,Chen YD,Podolsky RJ

更新日期：1975-04-01 00:00:00

abstract：:It is shown that alpha-helical content of eleven proteins is well correlated with alanine plus leucine content. These residues, taken singly or together, are to a first approximation randomly distributed in the four proteins whose tertiary structures have been determined (i.e., myoglobin, lysozyme, ribonuclease, alpha...

journal_title：Biophysical journal

authors： Prothero JW

更新日期：1968-11-01 00:00:00

abstract：:Transient receptor potential (TRP) channels are members of a large family of ion channels located in membranes rich in cholesterol, some of whose functions are affected by the cholesterol content of the membrane. Here, cholesterol binding to TRPs is studied using a docking procedure that allows the transmembrane surfa...

journal_title：Biophysical journal

authors： Lee AG

更新日期：2019-11-19 00:00:00

abstract：:Nanodiscs are an example of discoidal nanoscale self-assembled lipid/protein particles similar to nascent high-density lipoproteins, which reduce the risk of coronary artery disease. The major protein component of high-density lipoproteins is human apolipoprotein A-I, and the corresponding protein component of Nanodis...

journal_title：Biophysical journal

authors： Li Y,Kijac AZ,Sligar SG,Rienstra CM

更新日期：2006-11-15 00:00:00

abstract：:The insertion of myelin basic protein into microemulsion droplets of sodium bis (2-ethylhexyl) sulfosuccinate (AOT) has been studied by quasi-elastic light scattering. Measurements were made at both low and high molar ratios of water to surfactant, as a function of protein occupancy. The hydrodynamic radii of filled a...

journal_title：Biophysical journal

authors： Chatenay D,Urbach W,Cazabat AM,Vacher M,Waks M

更新日期：1985-12-01 00:00:00

abstract：:We propose a mechanism for the formation of membrane oscillations and traveling waves, which arise due to the coupling between the actin cytoskeleton and the calcium flux through the membrane. In our model, the fluid cell membrane has a mobile but constant population of proteins with a convex spontaneous curvature, wh...

journal_title：Biophysical journal

authors： Veksler A,Gov NS

更新日期：2009-09-16 00:00:00

abstract：:The barbiturate pentobarbital binds to gamma-aminobutyric acid type A (GABA(A)) receptors, and this interaction plays an important role in the anesthetic action of this drug. Depending on its concentration, pentobarbital can potentiate (approximately 10-100 microM), activate (approximately 100-800 microM), or block (a...

journal_title：Biophysical journal

authors： Muroi Y,Theusch CM,Czajkowski C,Jackson MB

更新日期：2009-01-01 00:00:00

abstract：:A simple theoretical analysis shows that specificity of double-stranded DNA (dsDNA) targeting by homopyrimidine peptide nucleic acids (hpyPNAs) is a kinetically controlled phenomenon. Our computations give the optimum conditions for sequence-specific targeting of dsDNA by hpyPNAs. The analysis shows that, in agreement...

journal_title：Biophysical journal

authors： Demidov VV,Yavnilovich MV,Frank-Kamenetskii MD

更新日期：1997-06-01 00:00:00

abstract：:We describe the use of asymmetric Ru-ligand complexes as a new class of luminescent probes that can be used to measure rotational motions of proteins. These complexes are known to display luminescent lifetimes ranging from 10 to 4000 ns. In this report, we show that the asymmetric complex Ru(bpy)2(dcbpy) (PF6)2 displa...

journal_title：Biophysical journal

authors： Terpetschnig E,Szmacinski H,Malak H,Lakowicz JR

更新日期：1995-01-01 00:00:00

abstract：:Mechanical and two-dimensional (2D) x-ray diffraction studies suggest that during isometric steady-state contraction, strongly bound cross-bridges mostly occupy early states in the power stroke, whereas rigor or rigor-like cross-bridges could not be detected. However, it remained unclear whether cross-bridges accumula...

journal_title：Biophysical journal

authors： Radocaj A,Weiss T,Helsby WI,Brenner B,Kraft T

更新日期：2009-02-18 00:00:00

abstract：:We have considered whether lecithin surface films below the gel-liquid crystal transition temperature, Tc, are in unique physical states. In general, below Tc, equilibrium films do not exist when surface pressures, pi, exceed about 0.1 dyn/cm. Since surface pressure-surface area isotherms of lecithin films below Tc al...

journal_title：Biophysical journal

authors： Horn LW,Gershfeld NL

更新日期：1977-06-01 00:00:00

abstract：:OVER A DECADE AND A HALF AGO, WHEN THE FIRST VISIBLE MEMBRANE SUSPENSION CIRCULAR DICHROIC (CD) SPECTRUM OF THE PURPLE MEMBRANE (PM) WAS PRESENTED, TWO MECHANISMS WERE PROPOSED TO ACCOUNT FOR THE OBSERVED BIPHASIC SHAPED CD BAND: (a) excitonic interactions among the retinals of the sole protein bacteriorhodopsin (bR) ...

journal_title：Biophysical journal

authors： Cassim JY

更新日期：1992-11-01 00:00:00

abstract：:The folding pathway of the small α/β protein GB1 has been extensively studied during the past two decades using both theoretical and experimental approaches. These studies provided a consensus view that the protein folds in a two-state manner. Here, we reassessed the folding of GB1, both by experiments and simulations...

journal_title：Biophysical journal

authors： Morrone A,Giri R,Toofanny RD,Travaglini-Allocatelli C,Brunori M,Daggett V,Gianni S

更新日期：2011-10-19 00:00:00

abstract：:Purple membranes were adsorbed on freestanding lipid bilayers, termed nano-black lipid membranes (nano-BLMs), suspending the pores of porous alumina substrates with average pore diameters of 280 nm. Nano-BLMs were obtained by first coating the upper surface of the highly ordered porous alumina substrates with a thin g...

journal_title：Biophysical journal

authors： Horn C,Steinem C

更新日期：2005-08-01 00:00:00

abstract：:Pore formation by membrane-active peptides, naturally encountered in innate immunity and infection, could have important medical and technological applications. Recently, the well-studied lytic peptide melittin has formed the basis for the development of combinatorial libraries from which potent pore-forming peptides ...

journal_title：Biophysical journal

authors： Sepehri A,PeBenito L,Pino-Angeles A,Lazaridis T

更新日期：2020-04-21 00:00:00

abstract：:Recently, it has become clear that the influenza virus fusion protein, hemagglutinin (HA), produces membrane destabilization and fusion by a multistep process, which involves the aggregation of the HAs to form a fusion site. While the details of this process are under debate, it is important to recognize that proposin...

journal_title：Biophysical journal

authors： Bentz J

更新日期：1992-08-01 00:00:00

abstract：:Purple photosynthetic bacteria harvest light using pigment-protein complexes which are often arranged in pseudo-organelles called chromatophores. A model of a chromatophore from Rhodospirillum photometricum was constructed based on atomic force microscopy data. Molecular-dynamics simulations and quantum-dynamics calcu...

journal_title：Biophysical journal

authors： Chandler DE,Strümpfer J,Sener M,Scheuring S,Schulten K

更新日期：2014-06-03 00:00:00

abstract：:Why is the spine of a neuron so small that it can contain only small numbers of molecules and reactions inevitably become stochastic? We previously showed that, despite such noisy conditions, the spine exhibits robust, sensitive, and efficient features of information transfer using the probability of Ca2+ increase; ho...

journal_title：Biophysical journal

authors： Fujii M,Ohashi K,Karasawa Y,Hikichi M,Kuroda S

更新日期：2017-02-28 00:00:00

abstract：:Analysis of trajectories of dynamical biological objects, such as breeding ants or cell organelles, is essential to reveal the interactions they develop with their environments. Many previous works used a global characterization based on parameters calculated for entire trajectories. In cases where transient behavior ...

journal_title：Biophysical journal

authors： Huet S,Karatekin E,Tran VS,Fanget I,Cribier S,Henry JP

更新日期：2006-11-01 00:00:00

abstract：:The electrophoretic behavior of defined DNA and RNA oligonucleotide duplexes from 10 to 20 bp in length has been investigated as a function of salt conditions, gel concentration, and temperature. The RNA oligomers migrated much more slowly than the DNA oligomers of the same sequence under all conditions. From sediment...

journal_title：Biophysical journal

authors： Bonifacio GF,Brown T,Conn GL,Lane AN

更新日期：1997-09-01 00:00:00

abstract：:The interactions between vitamin-A and beta-lactoglobulin have been investigated. We have found that two different complexes can be formed: one involving vitamin-A, and one involving a derivative of vitamin-A that most probably has a retro-beta-ionylidene structure. Room temperature absorption spectra for these comple...

journal_title：Biophysical journal

authors： Hemley R,Kohler BE,Siviski P

更新日期：1979-12-01 00:00:00

abstract：:ATP synthase (F-ATPase) operates as an electrochemical-to-mechanical-to-chemical energy transducer with an astounding 360 degrees rotary motion of subunits epsilongammac(10-14) (rotor) against delta(alphabeta)(3)ab(2) (stator). The enzyme's torque as a function of the angular reaction coordinate in relation to ATP-syn...

journal_title：Biophysical journal

authors： Cherepanov DA,Junge W

更新日期：2001-09-01 00:00:00

abstract：:Forces generated by microtubule polymerization and depolymerization are important for the biological functioning of cells. The mean growth velocity, V, under an opposing force, F, has been measured by; Science 278:856-860) for single microtubules growing in vitro, but their analysis of the data suggested that V decrea...

journal_title：Biophysical journal

authors： Kolomeisky AB,Fisher ME

更新日期：2001-01-01 00:00:00

abstract：:Our ability to understand speech requires neural tuning with high frequency resolution, but the peripheral mechanisms underlying sharp tuning in humans remain unclear. Sharp tuning in genetically modified mice has been attributed to decreases in spread of excitation of tectorial membrane traveling waves. Here we show ...

journal_title：Biophysical journal

authors： Farrahi S,Ghaffari R,Sellon JB,Nakajima HH,Freeman DM

更新日期：2016-09-06 00:00:00

abstract：:Single-molecule epifluorescence microscopy was used to observe the translational motion of GPI-linked and native I-E(k) class II MHC membrane proteins in the plasma membrane of CHO cells. The purpose of the study was to look for deviations from Brownian diffusion that might arise from barriers to this motion. Detergen...

journal_title：Biophysical journal

authors： Vrljic M,Nishimura SY,Brasselet S,Moerner WE,McConnell HM

更新日期：2002-11-01 00:00:00

abstract：:By employing classical molecular dynamics, correlation analysis of coupling between slow and fast dynamical modes, and free energy (umbrella) sampling using classical as well as mixed quantum mechanics molecular mechanics force fields, we uncover a possible pathway for phosphoryl transfer in the self-cleaving reaction...

journal_title：Biophysical journal

authors： Radhakrishnan R

更新日期：2007-10-01 00:00:00

abstract：:The effects of lysolipids on phospholipid layer curvature and bending elasticity were examined using x-ray diffraction and the osmotic stress method. Lysolipids with two different head groups, phosphatidylcholine (PC) and phosphatidylethanolamine (PE), and differing hydrocarbon chains were mixed with the hexagonal-for...

journal_title：Biophysical journal

authors： Fuller N,Rand RP

更新日期：2001-07-01 00:00:00

abstract：:Ca2+ release from the endoplasmic reticulum (ER) contributes to Ca2+ transients in frog sympathetic ganglion neurons. Here we use video-rate confocal fluo-4 fluorescence imaging to show that single action potentials reproducibly trigger rapidly rising Ca2+ transients at 1-3 local hot spots within the peripheral ER-ric...

journal_title：Biophysical journal

authors： Cseresnyés Z,Schneider MF

更新日期：2004-01-01 00:00:00

abstract：:A method for fabricating biomimetic surfaces from intact cell membranes is described. A monolayer of alkanethiol on gold is covered by a second layer derived from the components of erythrocyte membranes either by self-assembly or by Langmuir-Blodgett methods. The resulting asymmetric hybrid layer was characterized by ...

journal_title：Biophysical journal

authors： Rao NM,Plant AL,Silin V,Wight S,Hui SW

更新日期：1997-12-01 00:00:00

abstract：:Pure bending of a membrane bilayer is developed including different properties for each membrane half. Both connected and unconnected bilayer surfaces are treated. The bilayer bending resistance is the resultant of parallel surface compression "resistances." The neutral surface is a function of the upper and lower sur...

journal_title：Biophysical journal

authors： Evans EA

更新日期：1974-12-01 00:00:00