Nucleation and decay initiation are the stiffness-sensitive phases of focal adhesion maturation.

abstract：:Many experiments have measured the effect of force on the dissociation of single selectin bonds, but it is not yet clear how the force dependence of molecular dissociation can influence the rolling of cells expressing selectin molecules. Recent experiments using constant-force atomic force microscopy or high-resolutio...

journal_title：Biophysical journal

authors： Caputo KE,Lee D,King MR,Hammer DA

更新日期：2007-02-01 00:00:00

abstract：:The thylakoid membrane has a unique lipid composition, consisting mostly of galactolipids. These thylakoid lipids have important roles in photosynthesis. Here, we investigate to what extent these lipids bind specifically to the Photosystem II complex. To this end, we performed coarse-grain MD simulations of the Photos...

journal_title：Biophysical journal

authors： Van Eerden FJ,Melo MN,Frederix PWJM,Marrink SJ

更新日期：2017-12-19 00:00:00

abstract：:A detailed treatment is provided of the various free-energy terms that contribute to the transfer of a polyalanine alpha-helix from the aqueous phase into lipid bilayers. In agreement with previous work, the hydrophobic effect is found to provide the major driving force for helix insertion. However, an opposing effect...

journal_title：Biophysical journal

authors： Ben-Tal N,Ben-Shaul A,Nicholls A,Honig B

更新日期：1996-04-01 00:00:00

abstract：:The interaction of an amphiphilic, 40-amino acid beta-amyloid (Abeta) peptide with liposomal membranes as a function of sterol mole fraction (X(sterol)) was studied based on the fluorescence anisotropy of a site-specific membrane sterol probe, dehydroergosterol (DHE), and fluorescence resonance energy transfer (FRET) ...

journal_title：Biophysical journal

authors： Qiu L,Lewis A,Como J,Vaughn MW,Huang J,Somerharju P,Virtanen J,Cheng KH

更新日期：2009-05-20 00:00:00

abstract：:The morphological features of α-synuclein (AS) amyloid aggregation in vitro and in cells were elucidated at the nanoscale by far-field subdiffraction fluorescence localization microscopy. Labeling AS with rhodamine spiroamide probes allowed us to image AS fibrillar structures by fluorescence stochastic nanoscopy with ...

journal_title：Biophysical journal

authors： Roberti MJ,Fölling J,Celej MS,Bossi M,Jovin TM,Jares-Erijman EA

更新日期：2012-04-04 00:00:00

abstract：:The effects of lipid chain packing and permeant size and shape on permeability across lipid bilayers have been investigated in gel and liquid crystalline dipalmitoylphosphatidylcholine (DPPC) bilayers by a combined NMR line-broadening/dynamic light scattering method using seven short-chain monocarboxylic acids (formic...

journal_title：Biophysical journal

authors： Xiang TX,Anderson BD

更新日期：1998-12-01 00:00:00

abstract：:We study the folding of small proteins inside confining potentials. Proteins are described using an effective potential model that contains the Ramachandran angles as degrees of freedom and does not need any a priori information about the native state. Hydrogen bonds, dipole-dipole-, and hydrophobic interactions are t...

journal_title：Biophysical journal

authors： Ojeda P,Garcia ME,Londoño A,Chen NY

更新日期：2009-02-01 00:00:00

abstract：:We measure the organization and substructure of HT29 epithelial cells in a monolayer using angle-resolved low-coherence interferometry. This new technique probes cellular structure by measuring scattered light, as in flow cytometry, but offers an advantage in that the structure can be examined in situ, avoiding the ne...

journal_title：Biophysical journal

authors： Wax A,Yang C,Backman V,Badizadegan K,Boone CW,Dasari RR,Feld MS

更新日期：2002-04-01 00:00:00

abstract：:A free radical probe was introduced into single barnacle muscle cells, and its freedom of motion inferred from the spin resonance spectra. The probe reported an average local viscosity of 5-10 cp compared with 1 cp for pure water. From a comparison of the temperature dependence of the probe's tumbling rate in model aq...

journal_title：Biophysical journal

authors： Sachs F,Latorre R

更新日期：1974-04-01 00:00:00

abstract：:A system based on two designed peptides, namely the cationic peptide K, (KIAALKE)3, and its complementary anionic counterpart called peptide E, (EIAALEK)3, has been used as a minimal model for membrane fusion, inspired by SNARE proteins. Although the fact that docking of separate vesicle populations via the formation ...

journal_title：Biophysical journal

authors： Rabe M,Aisenbrey C,Pluhackova K,de Wert V,Boyle AL,Bruggeman DF,Kirsch SA,Böckmann RA,Kros A,Raap J,Bechinger B

更新日期：2016-11-15 00:00:00

abstract：:In this paper, we report on the presence of cation binding areas on bovine serum amine oxidase, where metal ions of the groups IA and IIA, such as Na(+), K(+), Cs(+), Mg(2+), and Ca(2+), bind with various affinities. We found a cation-binding area that influences the enzyme activity if occupied, so that the catalytic ...

journal_title：Biophysical journal

authors： Di Paolo ML,Scarpa M,Corazza A,Stevanato R,Rigo A

更新日期：2002-10-01 00:00:00

abstract：:Drug-membrane interactions are well known but poorly understood. Here we describe dual measurements of membrane thickness change and membrane area change due to the binding of the amphipathic drug curcumin. The combined results allowed us to analyze the binding states of a drug to lipid bilayers, one on the water-memb...

journal_title：Biophysical journal

authors： Sun Y,Lee CC,Hung WC,Chen FY,Lee MT,Huang HW

更新日期：2008-09-01 00:00:00

abstract：:The current article describes a new two-dimensional lambda-dynamics method to include proton tautomerism in continuous constant pH molecular dynamics (CPHMD) simulations. The two-dimensional lambda-dynamics framework is used to devise a tautomeric state titration model for the CPHMD simulations involving carboxyl and ...

journal_title：Biophysical journal

authors： Khandogin J,Brooks CL 3rd

更新日期：2005-07-01 00:00:00

abstract：:Nuclear calcium is a key signal in the dialogue between synapse and nucleus that controls the genomic responses required for persistent adaptations, including memory and acquired neuroprotection. The amplitude and duration of nuclear calcium transients specify activity-induced transcriptional changes. However, the pre...

journal_title：Biophysical journal

authors： Bengtson CP,Freitag HE,Weislogel JM,Bading H

更新日期：2010-12-15 00:00:00

abstract：:There is no methodology for the estimation of the dynamic features of large-molecular-weight RNAs in homogeneous physiological media. In this report, a luminescence anisotropy-based method using a long-lifetime luminescent oligonucleotide probe for the estimation of the dynamic features of large-molecular-weight RNA i...

journal_title：Biophysical journal

authors： Sakamoto T,Mahara A,Yamagata K,Iwase R,Yamaoka T,Murakami A

更新日期：2005-12-01 00:00:00

abstract：:Atomic force microscopy (AFM) can be used to probe the mechanics of molecular recognition between surfaces. In the application known as "chemical force" microscopy (CFM), a chemically modified AFM tip probes a surface through chemical recognition. When modified with a biological ligand or receptor, the AFM tip can dis...

journal_title：Biophysical journal

authors： Mazzola LT,Frank CW,Fodor SP,Mosher C,Lartius R,Henderson E

更新日期：1999-06-01 00:00:00

abstract：:This paper describes a molecular dynamics and molecular mechanics study of the solvation and selectivity of the narrow pore and vestibule region of a model-built structure for the voltage-gated sodium channel. The particular structure used was one proposed by Guy and Durell. However, many of the features we saw would ...

journal_title：Biophysical journal

authors： Singh C,Sankararamakrishnan R,Subramaniam S,Jakobsson E

更新日期：1996-11-01 00:00:00

abstract：:Nef is an HIV-1 accessory protein that directly contributes to AIDS progression. Nef is myristoylated on the N-terminus, associates with membranes, and may undergo a transition from a solution conformation to a membrane-associated conformation. It has been hypothesized that conformational rearrangement enables membran...

journal_title：Biophysical journal

authors： Kent MS,Murton JK,Sasaki DY,Satija S,Akgun B,Nanda H,Curtis JE,Majewski J,Morgan CR,Engen JR

更新日期：2010-09-22 00:00:00

abstract：:Phosphatidylinositol phospholipase Cγ (PLCγ) is an intracellular membrane-associated second-messenger signaling protein activated by tyrosine kinases such as fibroblast growth factor receptor 1. PLCγ contains the regulatory γ-specific array (γSA) comprising a tandem Src homology 2 (SH2) pair, an SH3 domain, and a spli...

journal_title：Biophysical journal

authors： Koss H,Bunney TD,Esposito D,Martins M,Katan M,Driscoll PC

更新日期：2018-07-03 00:00:00

abstract：:The analysis of peptide and protein partitioning in lipid membranes is of high relevance for the understanding of biomembrane function. We used statistical thermodynamics analysis to demonstrate the effect of peptide mixing behavior on heat capacity profiles of lipid membranes with the aim to predict peptide aggregati...

journal_title：Biophysical journal

authors： Ivanova VP,Makarov IM,Schäffer TE,Heimburg T

更新日期：2003-04-01 00:00:00

abstract：:Inositol 1,4,5-trisphosphate (InsP3) acts on intracellular receptors to cause liberation of Ca2+ ions into the cytosol as repetitive spikes and propagating waves. We studied the processes underlying this regenerative release of Ca2+ by monitoring with high resolution the kinetics of Ca2+ flux evoked in Xenopus oocytes...

journal_title：Biophysical journal

authors： Parker I,Yao Y,Ilyin V

更新日期：1996-01-01 00:00:00

abstract：:Cyclic GMP (cGMP) is the intracellular messenger mediating phototransduction in retinal rods, with its longitudinal diffusion in the rod outer segment (ROS) likely to be a factor in determining light sensitivity. From the kinetics of cGMP-activated currents in the truncated ROS of the salamander (Ambystoma tigrinum), ...

journal_title：Biophysical journal

authors： Koutalos Y,Brown RL,Karpen JW,Yau KW

更新日期：1995-11-01 00:00:00

abstract：:Migratory cells are known to adapt to environments that contain wide-ranging levels of chemoattractant. Although biochemical models of adaptation have been previously proposed, here, we discuss a different mechanism based on mechanosensing, in which the interaction between biochemical signaling and cell tension facili...

journal_title：Biophysical journal

authors： Zmurchok C,Collette J,Rajagopal V,Holmes WR

更新日期：2020-10-20 00:00:00

abstract：:A new general method for the determination of electrostatic potentials at biological surfaces is presented. The approach is based on measurement of the collision frequency of a charged nitroxide in solution with a nitroxide fixed to the surface at the point of interest. The collision frequency is determined with 14N:1...

journal_title：Biophysical journal

authors： Shin YK,Hubbell WL

更新日期：1992-06-01 00:00:00

abstract：:Cubic insulin crystals contain a binding site for monovalent cations in a cavity of the crystal dyad in which the bound cation is ligated by protein atomic dipoles and water molecules. These types of interaction are analogous to interactions that occur in small cation-selective carrier and channel molecules. X-ray dif...

journal_title：Biophysical journal

authors： Badger J,Kapulsky A,Gursky O,Bhyravbhatla B,Caspar DL

更新日期：1994-02-01 00:00:00

abstract：:Cardiac contraction and relaxation dynamics result from a set of simultaneously interacting Ca(2+) regulatory mechanisms. In this study, cardiocyte Ca(2+) dynamics were modeled using a set of six differential equations that were based on theories, equations, and parameters described in previous studies. Among the uniq...

journal_title：Biophysical journal

authors： Snyder SM,Palmer BM,Moore RL

更新日期：2000-07-01 00:00:00

abstract：:The membrane disruption mechanism of pandinin 1 (pin1), an antimicrobial peptide isolated from the venom of the African scorpion, was studied using 31P, 13C, 1H solid-state and multidimensional solution-state NMR spectroscopy. A high-resolution NMR solution structure of pin1 showed that the two distinct alpha-helical ...

journal_title：Biophysical journal

authors： Nomura K,Ferrat G,Nakajima T,Darbon H,Iwashita T,Corzo G

更新日期：2005-12-01 00:00:00

abstract：:Purified cytochrome c oxidase CO complex from beef heart has been studied by Fourier transform infrared absorbance difference spectroscopy. Photolysis at 10-20 Kelvin results in dissociation of a3FeCO, formation of CuBCO, and perturbation of the a3-heme and CuB complex. The vibrational perturbation spectrum between 90...

journal_title：Biophysical journal

authors： Park S,Pan LP,Chan SI,Alben JO

更新日期：1996-08-01 00:00:00

abstract：:We report here a method for the determination of the pKa of histidine in complex or heterogeneous systems amenable to neither solid-state nor solution NMR spectroscopy. Careful synthesis of a fluorenylmethyloxycarbonyl- and trityl-protected, C2-deuterated histidine produces a vibrational-probe-equipped amino acid that...

journal_title：Biophysical journal

authors： Braun GA,Pogostin BH,Pucetaite M,Londergan CH,Åkerfeldt KS

更新日期：2020-11-03 00:00:00

abstract：:An ab initio model for gene prediction in prokaryotic genomes is proposed based on physicochemical characteristics of codons calculated from molecular dynamics (MD) simulations. The model requires a specification of three calculated quantities for each codon: the double-helical trinucleotide base pairing energy, the b...

journal_title：Biophysical journal

authors： Singhal P,Jayaram B,Dixit SB,Beveridge DL

更新日期：2008-06-01 00:00:00