The effect of branching on the critical concentration and average filament length of actin.

abstract：:The detection of the 1H NMR signal of myoglobin (Mb) in tissue opens an opportunity to examine its cellular diffusion property, which is central to its purported role in facilitating oxygen transport. In perfused myocardium the field-dependent transverse relaxation analysis of the deoxy Mb proximal histidyl NdeltaH in...

journal_title：Biophysical journal

authors： Wang D,Kreutzer U,Chung Y,Jue T

更新日期：1997-11-01 00:00:00

abstract：:Interactions between millimeter waves (MMWs) and biological systems have received increasing attention due to the growing use of MMW radiation in technologies ranging from experimental medical devices to telecommunications and airport security. Studies have shown that MMW exposure alters cellular function, especially ...

journal_title：Biophysical journal

authors： Shapiro MG,Priest MF,Siegel PH,Bezanilla F

更新日期：2013-06-18 00:00:00

abstract：:Integrin-mediated adhesion of circulating neutrophils to endothelium during inflammation involves multiple adhesion molecules on both neutrophils and endothelium. Most studies of neutrophil adhesion have focused on adhesion to ICAM-1 (mediated by beta(2) integrins), but interaction with the endothelial ligand vascular...

journal_title：Biophysical journal

authors： Lomakina EB,Waugh RE

更新日期：2009-01-01 00:00:00

abstract：:An experimental method and analysis are introduced which provide direct quantitation of the strength of adhesive contact for large agglutinin-bonded regions between macroscopically smooth membrane capsules (e.g., red blood cells). The approach yields intrinsic properties for separation of adherent regions independent ...

journal_title：Biophysical journal

authors： Berk D,Evans E

更新日期：1991-04-01 00:00:00

abstract：:Molecular diffusion process after the photo-induced electron injection to ferric cytochrome c (Fe(III) cyt c) in guanidine hydrochloride (GdnHCl) 3.5 M buffer solution is studied by the time-resolved transient grating technique. Circular dichroism studies have revealed that Fe(III) cyt c is unfolded under this conditi...

journal_title：Biophysical journal

authors： Nada T,Terazima M

更新日期：2003-09-01 00:00:00

abstract：:The rate of muscle force redevelopment after release-restretch protocols has previously been interpreted using a simple two-state cross-bridge cycling model with rate constants for transitions between non-force-bearing and force-bearing states, f, and between force-bearing and non-force-bearing states, g. Changes in t...

journal_title：Biophysical journal

authors： Campbell K

更新日期：1997-01-01 00:00:00

abstract：:Benjamin Franklin, mostly known for his participation in writing The Declaration of Independence and work on electricity, was also one of the first scientists to seek to understand the properties of oil monolayers on water surfaces. During one of his many voyages across the Atlantic Ocean, Franklin observed that oil h...

journal_title：Biophysical journal

authors： Wang DN,Stieglitz H,Marden J,Tamm LK

更新日期：2013-01-22 00:00:00

abstract：:Antibodies are key tools in biomedical research and medicine. Their binding properties are classically measured in solution and characterized by an affinity. However, in physiological conditions, antibodies can bridge an immune effector cell and an antigen-presenting cell, implying that mechanical forces may apply to ...

journal_title：Biophysical journal

authors： González C,Chames P,Kerfelec B,Baty D,Robert P,Limozin L

更新日期：2019-04-23 00:00:00

abstract：:Synchrotron radiation circular dichroism, Fourier transform infrared, and nuclear magnetic resonance spectroscopies, and small-angle x-ray scattering were used to monitor the reversible thermal unfolding of hen egg white lysozyme. The results were compared with crystal structures and high- and low-temperature structur...

journal_title：Biophysical journal

authors： Meersman F,Atilgan C,Miles AJ,Bader R,Shang W,Matagne A,Wallace BA,Koch MH

更新日期：2010-10-06 00:00:00

abstract：:We investigate lateral organization of lipid domains in vesicles versus supported membranes and monolayers. The lipid mixtures used are predominantly DOPC/DPPC/Chol and DOPC/BSM/Chol, which have been previously shown to produce coexisting liquid phases in vesicles and monolayers. In a monolayer at an air-water interfa...

journal_title：Biophysical journal

authors： Stottrup BL,Veatch SL,Keller SL

更新日期：2004-05-01 00:00:00

abstract：:We present a truly quantitative fluorescence recovery after photobleaching (FRAP) model for use with the confocal laser scanning microscope based on the photobleaching of a long line segment. The line FRAP method is developed to complement the disk FRAP method reported before. Although being more subject to the influe...

journal_title：Biophysical journal

authors： Braeckmans K,Remaut K,Vandenbroucke RE,Lucas B,De Smedt SC,Demeester J

更新日期：2007-03-15 00:00:00

abstract：:We elucidate the physics of protein dynamical transition via 10-100-ns molecular dynamics simulations at temperatures spanning 160-300 K. By tracking the energy fluctuations, we show that the protein dynamical transition is marked by a crossover from nonstationary to stationary processes that underlie the dynamics of ...

journal_title：Biophysical journal

authors： Okan OB,Atilgan AR,Atilgan C

更新日期：2009-10-07 00:00:00

abstract：:The cytoskeletal activity of motile or adherent cells is frequently seen to induce detectable displacements of sufficiently compliant substrata. The physics of this phenomenon is discussed in terms of the classical theory of small-strain, plane-stress elasticity. The main results of such analysis is a transform expres...

journal_title：Biophysical journal

authors： Dembo M,Oliver T,Ishihara A,Jacobson K

更新日期：1996-04-01 00:00:00

abstract：:Methods are described for discrimination of models of the gating kinetics and permeation of single ionic channels. Both maximum likelihood and regression procedures are discussed. In simple situations, where models are nested, standard hypothesis tests can be used. More commonly, however, non-nested models are of inte...

journal_title：Biophysical journal

authors： Horn R

更新日期：1987-02-01 00:00:00

abstract：:The S4 segments of voltage-gated sodium channels are important parts of the voltage-sensing elements of these proteins. Furthermore, the addition of the isolated S4 polypeptide to planar lipid bilayers results in stepwise increases of ion conductivity. In order to gain insight into the mechanisms of pore formation by ...

journal_title：Biophysical journal

authors： Mattila K,Kinder R,Bechinger B

更新日期：1999-10-01 00:00:00

abstract：:Single-particle tracking is an important technique in the life sciences to understand the kinetics of biomolecules. The analysis of apparent diffusion coefficients in vivo, for example, enables researchers to determine whether biomolecules are moving alone, as part of a larger complex, or are bound to large cellular c...

journal_title：Biophysical journal

authors： Vink JNA,Brouns SJJ,Hohlbein J

更新日期：2020-11-17 00:00:00

abstract：:The characterization of the structural dynamics of proteins, including those that present a substantial degree of disorder, is currently a major scientific challenge. These dynamics are biologically relevant and govern the majority of functional and pathological processes. We exploited a combination of enhanced molecu...

journal_title：Biophysical journal

authors： Camilloni C,Schaal D,Schweimer K,Schwarzinger S,De Simone A

更新日期：2012-01-04 00:00:00

abstract：:Each myosin molecule contains two heavy chains and a total of four low-molecular weight light chain subunits, two "essential" and two "regulatory" light chains (RLCs). Although the roles of myosin light chains in vertebrate striated muscle are poorly understood at present, recent studies on the RLC have suggested that...

journal_title：Biophysical journal

authors： Diffee GM,Greaser ML,Reinach FC,Moss RL

更新日期：1995-04-01 00:00:00

abstract：:The membrane disruption mechanism of pandinin 1 (pin1), an antimicrobial peptide isolated from the venom of the African scorpion, was studied using 31P, 13C, 1H solid-state and multidimensional solution-state NMR spectroscopy. A high-resolution NMR solution structure of pin1 showed that the two distinct alpha-helical ...

journal_title：Biophysical journal

authors： Nomura K,Ferrat G,Nakajima T,Darbon H,Iwashita T,Corzo G

更新日期：2005-12-01 00:00:00

abstract：:Molecular recognition between biotinylated bovine serum albumin and polyclonal, biotin-directed IG antibodies has been measured directly under various buffer conditions using an atomic force microscope (AFM). It was found that even highly structured molecules such as IgG antibodies preserve their specific affinity to ...

journal_title：Biophysical journal

authors： Dammer U,Hegner M,Anselmetti D,Wagner P,Dreier M,Huber W,Güntherodt HJ

更新日期：1996-05-01 00:00:00

abstract：:The widely used hydrophobic cannabinoid ligand CP-55,940 partitions with high efficiency into biomembranes. We studied the location, orientation, and dynamics of CP-55,940 in POPC bilayers by solid-state NMR. Chemical-shift perturbation of POPC protons from the aromatic ring-current effect, as well as 1H NMR cross-rel...

journal_title：Biophysical journal

authors： Kimura T,Cheng K,Rice KC,Gawrisch K

更新日期：2009-06-17 00:00:00

abstract：:Molecular dynamics simulations of protein folding and unfolding are often carried out at temperatures (400-600 K) that are much higher than physiological or room temperature to speed up the (un)folding process. Use of such high temperatures changes both the protein and solvent properties considerably, compared to phys...

journal_title：Biophysical journal

authors： Walser R,Mark AE,van Gunsteren WF

更新日期：2000-06-01 00:00:00

abstract：:We study theoretically the feasibility of using transverse electronic transport within a nanopore for rapid DNA sequencing. Specifically, we examine the effects of the environment and detection probes on the distinguishability of the DNA bases. We find that the intrinsic measurement bandwidth of the electrodes helps t...

journal_title：Biophysical journal

authors： Lagerqvist J,Zwolak M,Di Ventra M

更新日期：2007-10-01 00:00:00

abstract：:Intracellular Ca2+ was determined with the fura-2 technique during electrically induced contractions in the rabbit rectococcygeus smooth muscle at 22 degreesC. The muscles were electrically activated to give short, reproducible contractions. Intracellular [Ca2+] increased during activation; the increase in [Ca2+] prec...

journal_title：Biophysical journal

authors： Arner A,Malmqvist U,Rigler R

更新日期：1998-10-01 00:00:00

abstract：:Fluorescent labels are often used in bioassays as a means to detect and characterize ligand-receptor binding. This is due in part to the inherently high sensitivity of fluorescence-based technology and the relative accessibility of the technique. There is often little concern raised as to whether or not the fluorescen...

journal_title：Biophysical journal

authors： Swift JL,Cramb DT

更新日期：2008-07-01 00:00:00

abstract：:Inositol 1,4,5-trisphosphate (InsP(3)) receptors (InsP(3)Rs) are intracellular Ca(2+) channels gated by the second messenger InsP(3). Here we describe a novel approach for recording single-channel currents through recombinant InsP(3)Rs in mammalian cells that applies patch-clamp electrophysiology to nuclei isolated fr...

journal_title：Biophysical journal

authors： Boehning D,Joseph SK,Mak DO,Foskett JK

更新日期：2001-07-01 00:00:00

abstract：:Voltage gated potassium channels open and inactivate in response to changes of the voltage across the membrane. After removal of the fast N-type inactivation, voltage gated Shaker K-channels (Shaker-IR) are still able to inactivate through a poorly understood closure of the ion conduction pore. This, usually slower, i...

journal_title：Biophysical journal

authors： Oliva C,González V,Naranjo D

更新日期：2005-08-01 00:00:00

abstract：:In rat mesenteric arteries, smooth muscle cells exhibit intercellular calcium waves in response to local phenylephrine stimulation. These waves have a velocity of approximately 20 cells/s and a range of approximately 80 cells. We analyze these waves in a theoretical model of a population of coupled smooth muscle cells...

journal_title：Biophysical journal

authors： Koenigsberger M,Seppey D,Bény JL,Meister JJ

更新日期：2010-07-21 00:00:00

abstract：:Previously (Hines, M., and J.J. Blum 1983, Biophys. J., 41:67-79), a method was developed that allowed one to compute curvature and twist for a three-dimensional sliding filament model. In that formalism it was difficult to specify the shear and bending moments arising from moment-bearing interfilament links such as f...

journal_title：Biophysical journal

authors： Hines M,Blum JJ

更新日期：1984-11-01 00:00:00

abstract：:Bin/Amphiphysin/Rvs-homology (BAR) domains generate and sense membrane curvature by binding the negatively charged membrane to their positively charged concave surfaces. N-BAR domains contain an N-terminal extension (helix-0) predicted to form an amphipathic helix upon membrane binding. We determined the NMR structure...

journal_title：Biophysical journal

authors： Löw C,Weininger U,Lee H,Schweimer K,Neundorf I,Beck-Sickinger AG,Pastor RW,Balbach J

更新日期：2008-11-01 00:00:00