Single-channel recordings of recombinant inositol trisphosphate receptors in mammalian nuclear envelope.

abstract：:Embryo and oocyte cryopreservation is a widely used technology for cryopreservation of genetic resources. One limitation of cryopreservation is the low tolerance to freezing observed for oocytes and embryos rich in lipid droplets. We apply Raman spectroscopy to investigate freezing of lipid droplets inside cumulus-ooc...

journal_title：Biophysical journal

authors： Okotrub KA,Mokrousova VI,Amstislavsky SY,Surovtsev NV

更新日期：2018-08-07 00:00:00

abstract：:A new microscopic technique is demonstrated that combines attributes from both near-field scanning optical microscopy (NSOM) and fluorescence resonance energy transfer (FRET). The method relies on attaching the acceptor dye of a FRET pair to the end of a near-field fiber optic probe. Light exiting the NSOM probe, whic...

journal_title：Biophysical journal

authors： Vickery SA,Dunn RC

更新日期：1999-04-01 00:00:00

abstract：:Cardiac contraction and relaxation dynamics result from a set of simultaneously interacting Ca(2+) regulatory mechanisms. In this study, cardiocyte Ca(2+) dynamics were modeled using a set of six differential equations that were based on theories, equations, and parameters described in previous studies. Among the uniq...

journal_title：Biophysical journal

authors： Snyder SM,Palmer BM,Moore RL

更新日期：2000-07-01 00:00:00

abstract：:The role of the extracellular Glu side chains of bacteriorhodopsin in the proton transport mechanism has been studied using the single mutants E9Q, E74Q, E194Q, and E204Q; the triple mutant E9Q/E194Q/E204Q; and the quadruple mutant E9Q/E74Q/E194Q/E204Q. Steady-state difference and deconvoluted Fourier transform infrar...

journal_title：Biophysical journal

authors： Lazarova T,Sanz C,Querol E,Padrós E

更新日期：2000-04-01 00:00:00

abstract：:Some plant leaves display complex, orientation-dependent, proton nuclear magnetic resonance (1H NMR) spectra. The spectral patterns vary as the angle between the leaf surface and the applied magnetic field is varied. They also vary with temperature and with the quantity of absorbed manganous ions, but they are indepen...

journal_title：Biophysical journal

authors： McCain DC,Markley JL

更新日期：1985-11-01 00:00:00

abstract：:Focal adhesion kinase (FAK) is a central focal adhesion protein that promotes focal adhesion turnover, but the role of FAK for cell mechanical stability is unknown. We measured the mechanical properties of wild-type (FAKwt), FAK-deficient (FAK-/-), FAK-silenced (siFAK), and siControl mouse embryonic fibroblasts by mag...

journal_title：Biophysical journal

authors： Fabry B,Klemm AH,Kienle S,Schäffer TE,Goldmann WH

更新日期：2011-11-02 00:00:00

abstract：:Large-conductance Ca(2+)-activated K(+) channels (BK) play a fundamental role in modulating membrane potential in many cell types. The gating of BK channels and its modulation by Ca(2+) and voltage has been the subject of intensive research over almost three decades, yielding several of the most complicated kinetic me...

journal_title：Biophysical journal

authors： Rosales RA,Varanda WA

更新日期：2009-05-20 00:00:00

abstract：:We have developed a model for the analysis of the forward creatine kinase reaction in muscle as measured by the nuclear magnetic resonance (NMR) technique of magnetization transfer. The model, accounting for the double-exponential behavior observed in some NMR magnetization transfer data, allows for the existence of t...

journal_title：Biophysical journal

authors： Zahler R,Bittl JA,Ingwall JS

更新日期：1987-06-01 00:00:00

abstract：:The model for carbon monoxide or dioxygen recombination with heme proteins developed by the group at the University of Illinois is reexamined. We propose that the carbon monoxide or dioxygen molecule enters the protein at essentially a diffusion-limited rate determined by the solvent viscosity and that the protein off...

journal_title：Biophysical journal

authors： Austin RH,Chan SS

更新日期：1978-10-01 00:00:00

abstract：:4',6-diamidino-2-phenylindole (DAPI), netropsin, and pentamidine are minor groove binders that have terminal -C(NH2)2+ groups. The hydration changes that accompany their binding to the minor groove of the (AATT)2 sequence have been studied using the osmotic stress technique with fluorescence spectroscopy. The affinity...

journal_title：Biophysical journal

authors： Degtyareva NN,Wallace BD,Bryant AR,Loo KM,Petty JT

更新日期：2007-02-01 00:00:00

abstract：:An understanding of the structure-dynamics relationship is essential for understanding how a protein works. Prior research has shown that the activity of a protein correlates with intradomain dynamics occurring at picosecond to millisecond timescales. However, the correlation between interdomain dynamics and the funct...

journal_title：Biophysical journal

authors： Kumar L,Nash A,Harms C,Planas-Iglesias J,Wright D,Klein-Seetharaman J,Sarkar SK

更新日期：2020-07-21 00:00:00

abstract：:We present a truly quantitative fluorescence recovery after photobleaching (FRAP) model for use with the confocal laser scanning microscope based on the photobleaching of a long line segment. The line FRAP method is developed to complement the disk FRAP method reported before. Although being more subject to the influe...

journal_title：Biophysical journal

authors： Braeckmans K,Remaut K,Vandenbroucke RE,Lucas B,De Smedt SC,Demeester J

更新日期：2007-03-15 00:00:00

abstract：:2D-ELDOR spectroscopy has been employed to study the dynamic structure of the liquid-ordered (Lo) phase versus that of the liquid-crystalline (Lc) phase in multibilayer phospholipid vesicles without (Lc) and with (Lo) cholesterol, using end-chain and headgroup labels and spin-labeled cholestane. The spectra are in mos...

journal_title：Biophysical journal

authors： Costa-Filho AJ,Shimoyama Y,Freed JH

更新日期：2003-04-01 00:00:00

abstract：:We have previously observed that the hydrophobic polyelectrolyte poly(2-ethylacrylic acid) solubilizes lipid membranes in a pH-dependent manner, and we have exploited this phenomenon to prepare lipid vesicles that release their contents in response to pH, light, or glucose (Thomas, J. L., and D. A. Tirrell. Acc. Chem....

journal_title：Biophysical journal

authors： Thomas JL,Barton SW,Tirrell DA

更新日期：1994-09-01 00:00:00

abstract：:Many proteins "bind" DNA through positively charged amino acids on their surfaces. However, to overcome significant energetic and topological obstacles, proteins that bend or package DNA might also modulate the stiffness that is generated by repulsions between phosphates within DNA. Much previous work describes how io...

journal_title：Biophysical journal

authors： Podestà A,Indrieri M,Brogioli D,Manning GS,Milani P,Guerra R,Finzi L,Dunlap D

更新日期：2005-10-01 00:00:00

abstract：:We have examined the distribution of ryanodine receptors, L-type Ca(2+) channels, calsequestrin, Na(+)/Ca(2+) exchangers, and voltage-gated Na(+) channels in adult rat ventricular myocytes. Enzymatically dissociated cells were fixed and dual-labeled with specific antibodies using standard immunocytochemistry protocols...

journal_title：Biophysical journal

authors： Scriven DR,Dan P,Moore ED

更新日期：2000-11-01 00:00:00

abstract：:Hair cells of the inner ear can power spontaneous oscillations of their mechanosensory hair bundle, resulting in amplification of weak inputs near the characteristic frequency of oscillation. Recently, dynamic force measurements have revealed that delayed gating of the mechanosensitive ion channels responsible for mec...

journal_title：Biophysical journal

authors： Barral J,Jülicher F,Martin P

更新日期：2018-01-23 00:00:00

abstract：:Directly measured forces between DNA helices in ordered arrays have been reduced to simple force coefficients and mathematical expressions for the interactions between pairs of molecules. The tabulated force parameters and mathematical expressions can be applied to parallel molecules or, by transformation, to skewed m...

journal_title：Biophysical journal

authors： Podgornik R,Rau DC,Parsegian VA

更新日期：1994-04-01 00:00:00

abstract：:We present a single virion method to determine absolute distributions of copy number in the protein composition of viruses and apply it to herpes simplex virus type 1. Using two-color coincidence fluorescence spectroscopy, we determine the virion-to-virion variability in copy numbers of fluorescently labeled tegument ...

journal_title：Biophysical journal

authors： Clarke RW,Monnier N,Li H,Zhou D,Browne H,Klenerman D

更新日期：2007-08-15 00:00:00

abstract：:In this review, we focus on the early events in the process of fibroblast spreading on fibronectin matrices of different rigidities. We present a focused position piece that illustrates the many different tests that a cell makes of its environment before it establishes mature matrix adhesions. When a fibroblast is pla...

journal_title：Biophysical journal

authors： Wolfenson H,Iskratsch T,Sheetz MP

更新日期：2014-12-02 00:00:00

abstract：:Homooligomerization of proline utilization A (PutA) bifunctional flavoenzymes is intimately tied to catalytic function and substrate channeling. PutA from Bradyrhizobium japonicum (BjPutA) is unique among PutAs in that it forms a tetramer in solution. Curiously, a dimeric BjPutA hot spot mutant was previously shown to...

journal_title：Biophysical journal

authors： Korasick DA,Campbell AC,Christgen SL,Chakravarthy S,White TA,Becker DF,Tanner JJ

更新日期：2018-06-19 00:00:00

abstract：:Fibrin polymerizes to produce branching fibers forming a three-dimensional network, which has been difficult to visualize by conventional microscopy. Three-dimensional images of whole clots at high resolution were obtained from stereo-pair intermediate-voltage electron micrographs. Computer software was developed to p...

journal_title：Biophysical journal

authors： Baradet TC,Haselgrove JC,Weisel JW

更新日期：1995-04-01 00:00:00

abstract：:The temperature and voltage dependence of gating and conductance of sarcoplasmic reticulum K+ channels (S-R K+) isolated from adult canine hearts were studied using the reconstituted bilayer technique. Fusion of vesicles from this preparation frequently resulted in the incorporation of a single channel. Only bilayers ...

journal_title：Biophysical journal

authors： Shen WK,Rasmusson RL,Liu QY,Crews AL,Strauss HC

更新日期：1993-08-01 00:00:00

abstract：:Green flagellates possess rhodopsin-like photoreceptors involved in control of their behavior via generation of photocurrents across the plasma membrane. Chlamydomonas mutants blocked in retinal biosynthesis are "blind," but they can be rescued by the addition of exogenous retinoids. Photosignaling by chlamyrhodopsin ...

journal_title：Biophysical journal

authors： Govorunova EG,Sineshchekov OA,Gärtner W,Chunaev AS,Hegemann P

更新日期：2001-11-01 00:00:00

abstract：:Previously, we discussed the calculation of the dipole moments of small proteins using the three-dimensional protein data-base. Our results demonstrate that the calculated dipole moments are in acceptable agreement with measured values. We, however, noted the difficulty of the calculation with larger proteins, in part...

journal_title：Biophysical journal

authors： Takashima S

更新日期：1993-05-01 00:00:00

abstract：:The influence of electrostatic multipole moments up to hexadecapole on the dynamics of photodissociated carbon monoxide (CO) in myoglobin is investigated. The CO electrostatic potential is expressed as an expansion into atomic multipole moments of increasing order up to octopole which are obtained from a distributed m...

journal_title：Biophysical journal

authors： Plattner N,Meuwly M

更新日期：2008-04-01 00:00:00

abstract：:In this new method for studying the shape recovery of deformed red blood cells, three optical traps ("optical tweezers") induce a parachute-shaped red cell deformation, which is comparable to the deformation in small capillaries. The shape recovery is recorded, and a relaxation time is obtained for each individual red...

journal_title：Biophysical journal

authors： Bronkhorst PJ,Streekstra GJ,Grimbergen J,Nijhof EJ,Sixma JJ,Brakenhoff GJ

更新日期：1995-11-01 00:00:00

abstract：:Communication-deficient cells (the SKHep1 cell line) were stably transfected with a plasmid containing cDNA which encodes the major gap junction protein of rat liver, connexin32. Application of the dual whole-cell voltage clamp technique with patch electrodes to pairs of transfected SKHep1 cells revealed strong sensit...

journal_title：Biophysical journal

authors： Moreno AP,Eghbali B,Spray DC

更新日期：1991-11-01 00:00:00

abstract：:Flagellin is the subunit of the bacterial filament, the micrometer-long propeller of a bacterial flagellum. The protein is believed to undergo unfolding for transport through the channel of the filament and to refold in a chamber at the end of the channel before being assembled into the growing filament. We report a t...

journal_title：Biophysical journal

authors： Chng CP,Kitao A

更新日期：2008-05-15 00:00:00

abstract：:Entropy Sampling Monte Carlo (ESMC) simulations were carried out to study the thermodynamics of the folding transition in the GCN4 leucine zipper (GCN4-lz) in the context of a reduced model. Using the calculated partition functions for the monomer and dimer, and taking into account the equilibrium between the monomer ...

journal_title：Biophysical journal

authors： Mohanty D,Kolinski A,Skolnick J

更新日期：1999-07-01 00:00:00