Adaptive Response of Actin Bundles under Mechanical Stress.

abstract：:A kinetic model is proposed to reproduce the time courses of the concentration change in paused leader RNA, terminated leader RNA, and readthrough RNA in the single-round transcription experiments on trp leader region of Escherichia coli and its mutants, L132, L75, and L75L135 (Winkler, M. E., and C. Yanofsky, 1981, B...

journal_title：Biophysical journal

authors： Suzuki H,Kunisawa T,Otsuka J

更新日期：1987-03-01 00:00:00

abstract：:Nuclear calcium is a key signal in the dialogue between synapse and nucleus that controls the genomic responses required for persistent adaptations, including memory and acquired neuroprotection. The amplitude and duration of nuclear calcium transients specify activity-induced transcriptional changes. However, the pre...

journal_title：Biophysical journal

authors： Bengtson CP,Freitag HE,Weislogel JM,Bading H

更新日期：2010-12-15 00:00:00

abstract：:The muscle nicotinic receptor (AChR) is a pentamer of four different subunits, each of which contains four transmembrane domains (M1-M4). We recently showed that channel opening and closing rates of the AChR depend on a hydrogen bond involving a threonine at position 14' of the M4 domain in the alpha-subunit. To deter...

journal_title：Biophysical journal

authors： Bouzat C,Gumilar F,del Carmen Esandi M,Sine SM

更新日期：2002-04-01 00:00:00

abstract：:Isothermal (27 degrees C) phase behavior of dimyristoyl phosphatidyl choline-cholesterol mixtures at various osmotic pressures and cholesterol contents was investigated by means of isothermal sorption microcalorimetry and (2)H-nuclear magnetic resonance. The calorimetric method allows for simultaneous measurement of t...

journal_title：Biophysical journal

authors： Sparr E,Hallin L,Markova N,Wennerström H

更新日期：2002-10-01 00:00:00

abstract：:Microscopic calcium (Ca2+) events (such as Ca2+ sparks) are an important area for study, as they help clarify the mechanism(s) underlying intracellular signaling. In the heart, Ca2+ sparks occur as a result of Ca2+ release from the sarcoendoplasmic reticulum, via ryanodine receptor channels. Measurement of Ca2+ spark ...

journal_title：Biophysical journal

authors： Kong CH,Soeller C,Cannell MB

更新日期：2008-12-15 00:00:00

abstract：:An interactive program is described for calculating the second virial coefficient contribution to the thermodynamic nonideality of solutions of rigid macromolecules based on their triaxial dimensions. The FORTRAN-77 program, available in precompiled form for the PC, is based on theory for the covolume of triaxial elli...

journal_title：Biophysical journal

authors： Harding SE,Horton JC,Jones S,Thornton JM,Winzor DJ

更新日期：1999-05-01 00:00:00

abstract：:Small angle x-ray and neutron scattering are techniques that give solution structures for large macromolecules. The creation of physically realistic atomistic models from known high-resolution structures to determine joint x-ray and neutron scattering best-fit structures offers a, to our knowledge, new method that sig...

journal_title：Biophysical journal

authors： Wright DW,Elliston ELK,Hui GK,Perkins SJ

更新日期：2019-12-03 00:00:00

abstract：:In this article, we demonstrate the new method of pulsed interleaved excitation (PIE), which can be used to extend the capabilities of multiple-color fluorescence imaging, fluorescence cross-correlation spectroscopy (FCCS), and single-pair fluorescence resonance energy transfer (spFRET) measurements. In PIE, multiple ...

journal_title：Biophysical journal

authors： Müller BK,Zaychikov E,Bräuchle C,Lamb DC

更新日期：2005-11-01 00:00:00

abstract：:The time correlation function of the shape fluctuations of large (greater than 10 micron), cylindrical, hydrated, phospholipid-membrane vesicles consisting of one bimolecular layer was measured. The restoring force of the membrane was due to the excess curvature of a membrane element. A value for the curvature elastic...

journal_title：Biophysical journal

authors： Schneider MB,Jenkins JT,Webb WW

更新日期：1984-05-01 00:00:00

abstract：:The formation of linear protein fibrils has previously been shown to be enhanced by volume exclusion or crowding in the presence of a high concentration of chemically inert protein or polymer, and by adsorption to membrane surfaces. An equilibrium mesoscopic model for the combined effect of both crowding and adsorptio...

journal_title：Biophysical journal

authors： Hoppe T,Minton AP

更新日期：2015-02-17 00:00:00

abstract：:Viral infection begins with the binding of a virus to a specific target on the surface of the host cell, followed by viral genome delivery into the host and a continuation of the infection process. Before binding occurs, the virus must first find its receptor by a process whose details are largely unknown. We applied ...

journal_title：Biophysical journal

authors： Rothenberg E,Sepúlveda LA,Skinner SO,Zeng L,Selvin PR,Golding I

更新日期：2011-06-22 00:00:00

abstract：:Tet repressor (TetR) is involved in the most abundant mechanism of tetracycline (Tc) resistance of gram-negative bacteria. Raman spectra were measured for the class D TetR protein, for an oligodeoxyribonucleotide with sequence corresponding to operator site O1, and for the TetR:oligonucleotide complex. TetR forms a co...

journal_title：Biophysical journal

authors： Krafft C,Hinrichs W,Orth P,Saenger W,Welfle H

更新日期：1998-01-01 00:00:00

abstract：:Omega-3 polyunsaturated fatty acids (n-3 PUFA), enriched in fish oils, are increasingly recognized to have potential benefits for treating many human afflictions. Despite the importance of PUFA, their molecular mechanism of action remains unclear. One emerging hypothesis is that phospholipids containing n-3 PUFA acyl ...

journal_title：Biophysical journal

authors： Williams JA,Batten SE,Harris M,Rockett BD,Shaikh SR,Stillwell W,Wassall SR

更新日期：2012-07-18 00:00:00

abstract：:The organization of the normal collagen molecule and fibrils is reviewed and the detection, assay, and isolation of a collagenolytic enzyme from amphibian tadpole tissue are described and its possible significance in metamorphosis is discussed ...

journal_title：Biophysical journal

authors： GROSS J

更新日期：1964-01-01 00:00:00

abstract：:Quantitative relationships between inputs and outputs of signaling systems are fundamental information for the understanding of the mechanism of signal transduction. Here we report the correlation between the number of epidermal growth factor (EGF) bindings and the response probability of intracellular calcium elevati...

journal_title：Biophysical journal

authors： Uyemura T,Takagi H,Yanagida T,Sako Y

更新日期：2005-05-01 00:00:00

abstract：:In adherent and motile neutrophils NAD(P)H concentration, flavoprotein redox potential, and production of reactive oxygen species and nitric oxide, are all periodic and exhibit defined phase relationships to an underlying metabolic oscillation of approximately 20 s. Utilizing fluorescence microscopy, we have shown in ...

journal_title：Biophysical journal

authors： Rosenspire AJ,Kindzelskii AL,Simon BJ,Petty HR

更新日期：2005-05-01 00:00:00

abstract：:A triple mutant of sperm whale myoglobin (Mb) [Leu(B10) --> Tyr, His(E7) --> Gln, and Thr(E10) --> Arg, called Mb-YQR], investigated by stopped-flow, laser photolysis, crystallography, and molecular dynamics (MD) simulations, proved to be quite unusual. Rebinding of photodissociated NO, O2, and CO from within the prot...

journal_title：Biophysical journal

authors： Brunori M,Cutruzzolà F,Savino C,Travaglini-Allocatelli C,Vallone B,Gibson QH

更新日期：1999-03-01 00:00:00

abstract：:hbAP0 is a model membrane protein designed to possess an anesthetic-binding cavity in its hydrophilic domain and a cation channel in its hydrophobic domain. Grazing incidence x-ray diffraction shows that hbAP0 forms four-helix bundles that are vectorially oriented within Langmuir monolayers at the air-water interface....

journal_title：Biophysical journal

authors： Churbanova IY,Tronin A,Strzalka J,Gog T,Kuzmenko I,Johansson JS,Blasie JK

更新日期：2006-05-01 00:00:00

abstract：:We accelerate protein folding in all-atom molecular dynamics simulations by introducing alternating hydrogen bond potentials as a supplement to the force field. The alternating hydrogen bond potentials result in accelerated hydrogen bond reordering, which leads to rapid formation of secondary structure elements. The m...

journal_title：Biophysical journal

authors： Wolf MG,de Leeuw SW

更新日期：2008-05-15 00:00:00

abstract：:Spin inversion transfer (SIT) NMR experiments are reported probing the thermodynamics and kinetics of interconversion of two folded forms of a GCN4-like leucine zipper near room temperature. The peptide is 13Calpha-labeled at position V9(a) and results are compared with prior findings for position L13(e). The SIT data...

journal_title：Biophysical journal

authors： d'Avignon DA,Bretthorst GL,Holtzer ME,Holtzer A

更新日期：1999-05-01 00:00:00

abstract：:Immobilization is a key step involved in probing molecular interactions using single-molecule force spectroscopy methods, including atomic force microscopy (AFM). To our knowledge, we describe a novel approach termed flexible nanoarray (FNA) in which the interaction between the two internally immobilized amyloid β pep...

journal_title：Biophysical journal

authors： Krasnoslobodtsev AV,Zhang Y,Viazovkina E,Gall A,Bertagni C,Lyubchenko YL

更新日期：2015-05-05 00:00:00

abstract：:The stability of the structure of double-stranded DNA in the salt-free solution is discussed on the basis of the polyelectrolyte theory. Assuming that DNA is an infinitely long rod, and the formation of double strands is divided into combining process and folding process, the free energy changes required in these proc...

journal_title：Biophysical journal

authors： OHNISHI T

更新日期：1963-11-01 00:00:00

abstract：:During protein synthesis dictated by the codon sequence of messenger RNA, the ribosome selects aminoacyl-tRNA (aa-tRNA) with high accuracy, the exact mechanism of which remains elusive. By using a single-molecule fluorescence resonance energy transfer method coupled with fluorescence emission anisotropy, we provide ev...

journal_title：Biophysical journal

authors： Mishra PP,Qureshi MT,Ren W,Lee TH

更新日期：2010-12-01 00:00:00

abstract：:Neutron diffraction from oriented multibilayers has been used to study the bilayer interaction of the amphipathic peptide salmon calcitonin. Penetration of calcitonin into bilayers composed of dioleoylphosphatidylcholine increases with the addition of 15% (mol) of the anionic phospholipid dioleoylphosphatidylglycerol....

journal_title：Biophysical journal

authors： Bradshaw JP

更新日期：1997-05-01 00:00:00

abstract：:4',6-diamidino-2-phenylindole (DAPI), netropsin, and pentamidine are minor groove binders that have terminal -C(NH2)2+ groups. The hydration changes that accompany their binding to the minor groove of the (AATT)2 sequence have been studied using the osmotic stress technique with fluorescence spectroscopy. The affinity...

journal_title：Biophysical journal

authors： Degtyareva NN,Wallace BD,Bryant AR,Loo KM,Petty JT

更新日期：2007-02-01 00:00:00

abstract：:We have obtained clear evidence for the flexibility of native scallop adductor thin filaments by studying the temperature and ionic strength dependence of the average decay constants obtained from intensity fluctuation spectroscopic (IFS) measurements. The low-angle (10-25 degrees ), average decay constants obtained f...

journal_title：Biophysical journal

authors： Newman J,Carlson FD

更新日期：1980-01-01 00:00:00

abstract：:Excised inside-out membrane patches are useful for studying the cGMP-activated ion channels that generate the electrical response to light in retinal rod cells. We show that strong ionic current across a patch changes the driving force on the current by altering the ionic concentration near the surface membrane, an ef...

journal_title：Biophysical journal

authors： Zimmerman AL,Karpen JW,Baylor DA

更新日期：1988-08-01 00:00:00

abstract：:The lateral distribution of N-[10(1-pyrenyl)decanoyl]-sphingomyelin (PyrSPM) and N-[10(1-pyrenyl)decanoyl]-glucocerebroside (PyrGlcCer) was studied in multilamellar vesicles of 1,2-dipalmitoyl-, 1,2-dimyristoyl-, and 1-palmitoyl-2-oleoyl-phosphatidylcholine (DPPC, DMPC, and POPC, respectively) under anaerobic conditio...

journal_title：Biophysical journal

authors： Hresko RC,Sugár IP,Barenholz Y,Thompson TE

更新日期：1987-05-01 00:00:00

abstract：:Two-photon excitation (TPE) spectra of Fura-2, -4F, -6F, -FF, and Furaptra were characterized using a tunable (750-850 nM) ultra-short pulse laser. Two-photon fluorescence of these dyes was studied in free solution and in the cytosol of isolated rabbit ventricular cardiomyocytes. The TPE spectra of the Ca(2+)-free and...

journal_title：Biophysical journal

authors： Wokosin DL,Loughrey CM,Smith GL

更新日期：2004-03-01 00:00:00

abstract：:Cyclic 3'5' adenosine monophosphate (cAMP)-dependent-protein kinase (PKA) signaling is a fundamental regulatory pathway for mediating cellular responses to hormonal stimuli. The pathway is activated by high-affinity association of cAMP with the regulatory subunit of PKA and signal termination is achieved upon cAMP dis...

journal_title：Biophysical journal

authors： Krishnamurthy S,Moorthy BS,Xin Xiang L,Xin Shan L,Bharatham K,Tulsian NK,Mihalek I,Anand GS

更新日期：2014-09-16 00:00:00