Direct visualization of spruce budworm antifreeze protein interacting with ice crystals: basal plane affinity confers hyperactivity.

abstract：:Ion channels assist and control the diffusion of ions through biological membranes. The conduction process depends on the structural characteristics of these nanopores, among which are the hydrophobicity and the afforded diameter of the conduction pathway. In this contribution, we use full atomistic free-energy molecu...

journal_title：Biophysical journal

authors： Treptow W,Tarek M

更新日期：2006-08-01 00:00:00

abstract：:Drug-membrane interactions are well known but poorly understood. Here we describe dual measurements of membrane thickness change and membrane area change due to the binding of the amphipathic drug curcumin. The combined results allowed us to analyze the binding states of a drug to lipid bilayers, one on the water-memb...

journal_title：Biophysical journal

authors： Sun Y,Lee CC,Hung WC,Chen FY,Lee MT,Huang HW

更新日期：2008-09-01 00:00:00

abstract：:The steady-state stimulus-response curve of the Limulus ventral photoreceptor comprises a linear portion followed by a less-than-unity power law dependence, which is maintained over at least 4 decades of intensity. This progressive desensitization corresponds to light adaptation. For flash stimulation of dark-adapted ...

journal_title：Biophysical journal

authors： Grzywacz NM,Hillman P

更新日期：1988-03-01 00:00:00

abstract：:We have measured, using infrared spectroscopy, the hydrogen/deuterium exchange rates of the amide protons in the photosynthetic antenna of Rhodospirillum rubrum. These measurements were made not only on the intact protein in detergent solution but also on two dissociated forms (B820 and B777). We have, on the basis of...

journal_title：Biophysical journal

authors： Sturgis J,Robert B,Goormaghtigh E

更新日期：1998-02-01 00:00:00

abstract：:The structural features of SPM that control the transbilayer distribution of beta-GalCer in POPC vesicles were investigated by (13)C- and (31)P-NMR spectroscopy using lipid analogs that share physical similarities with GalCer or SPM. The SPM analogs included N-palmitoyl-4,5-dihydro-SPM, 3-deoxy-SPM, 1-alkyl-2-amidopho...

journal_title：Biophysical journal

authors： Malewicz B,Valiyaveettil JT,Jacob K,Byun HS,Mattjus P,Baumann WJ,Bittman R,Brown RE

更新日期：2005-04-01 00:00:00

abstract：:Cofilin/ADF, beryllium fluoride complex (BeFx), and phalloidin have opposing effects on actin filament structure and dynamics. Cofilin/ADF decreases the stability of F-actin by enhancing disorder in subdomain 2, and by severing and accelerating the depolymerization of the filament. BeFx and phalloidin stabilize the su...

journal_title：Biophysical journal

authors： Muhlrad A,Ringel I,Pavlov D,Peyser YM,Reisler E

更新日期：2006-12-15 00:00:00

abstract：:The interactions of the hydrophobic pulmonary surfactant proteins SP-B and SP-C with 1,2-dipalmitoylphosphatidylcholine in mixed, spread monolayer films have been studied in situ at the air/water interface with the technique of external reflection absorption infrared spectroscopy (IRRAS). SP-C has a mostly alpha-helic...

journal_title：Biophysical journal

authors： Pastrana-Rios B,Taneva S,Keough KM,Mautone AJ,Mendelsohn R

更新日期：1995-12-01 00:00:00

abstract：:Eukaryotic DNA is packaged into the cell nucleus as a nucleoprotein complex, chromatin. Despite this condensed state, access to the DNA sequence must occur during gene expression and other essential genetic events. Here we employ optical tweezers stretching of reconstituted chromatin fibers to investigate the release ...

journal_title：Biophysical journal

authors： Pope LH,Bennink ML,van Leijenhorst-Groener KA,Nikova D,Greve J,Marko JF

更新日期：2005-05-01 00:00:00

abstract：:Concentrated solutions of bovine beta-lactoglobulin were studied using osmotic stress and rheological techniques. At pH 6.0 and 8.0, the osmotic pressure was largely independent of NaCl concentration and could be described by a hard sphere equation of state. At pH 5.1, close to the isoelectric point, the osmotic press...

journal_title：Biophysical journal

authors： Parker R,Noel TR,Brownsey GJ,Laos K,Ring SG

更新日期：2005-08-01 00:00:00

abstract：:A pulsed source neutron spectrometer has been used to measure vibrational spectra (20-4000 cm-1) of dry and hydrated type I collagen fibers, and of two model polypeptides, polyproline II and (prolyl-prolyl-glycine)10, at temperatures of 30 and 120 K. the collagen spectra provide the first high resolution neutron views...

journal_title：Biophysical journal

authors： Middendorf HD,Hayward RL,Parker SF,Bradshaw J,Miller A

更新日期：1995-08-01 00:00:00

abstract：:We have used chemical synthesis, electron paramagnetic resonance (EPR), and circular dichroism to detect and analyze the structural dynamics of a ryanodine receptor (RyR) peptide bound to calmodulin (CaM). The skeletal muscle calcium release channel RyR1 is activated by Ca2+-free CaM and inhibited by Ca2+-bound CaM. T...

journal_title：Biophysical journal

authors： Her C,McCaffrey JE,Thomas DD,Karim CB

更新日期：2016-12-06 00:00:00

abstract：:The conformation of mononucleosome complexes reconstituted with recombinant core histones on a 614-basepair-long DNA fragment containing the Xenopus borealis 5S rRNA nucleosome positioning sequence was studied by scanning/atomic force microscopy in the absence or presence of linker histone H1. Imaging without prior fi...

journal_title：Biophysical journal

authors： Kepert JF,Tóth KF,Caudron M,Mücke N,Langowski J,Rippe K

更新日期：2003-12-01 00:00:00

abstract：:A phenomenological theory for contracting muscle based on irreversible thermodynamics and the sliding filament theory is developed. The individual cross bridges, considered as subunits, are viewed as linear energy converters with constant transport coefficients. With this view of the subunits, phenomenological equatio...

journal_title：Biophysical journal

authors： Bornhorst WJ,Minardi JE

更新日期：1970-02-01 00:00:00

abstract：:Staphylococcus aureus expresses numerous virulence factors that aid in immune evasion. The four-domain staphylococcal immunoglobulin binding (Sbi) protein interacts with complement component 3 (C3) and its thioester domain (C3d)-containing fragments. Recent structural data suggested two possible modes of binding of Sb...

journal_title：Biophysical journal

authors： Gorham RD Jr,Rodriguez W,Morikis D

更新日期：2014-03-04 00:00:00

abstract：:The dependence of phospholipid vesicle size on lipid composition is investigated by photon correlation spectroscopy. For each lipid composition prolonged ultracentrifugation was used to isolate a nearly uniform population of minimum-sized vesicles. The residual size variations in the samples were sufficient to cause p...

journal_title：Biophysical journal

authors： Goll J,Carlson FD,Barenholz Y,Litman BJ,Thompson TE

更新日期：1982-04-01 00:00:00

abstract：:Leukocyte capture and rolling on the vascular endothelium is mediated principally by the selectin family of cell adhesion receptors. In a parallel plate flow chamber, neutrophil rolling on purified selectins or a selectin-ligand substrate was resolved by high speed videomicroscopy as a series of ratchet-like steps wit...

journal_title：Biophysical journal

authors： Smith MJ,Berg EL,Lawrence MB

更新日期：1999-12-01 00:00:00

abstract：:The effect of peptides, corresponding to sequences in the skeletal muscle dihydropyridine receptor II-III loop, on Ca(2+) release from sarcoplasmic reticulum (SR) and on ryanodine receptor (RyR) calcium release channels have been compared in preparations from normal and malignant hyperthermia (MH)-susceptible pigs. Pe...

journal_title：Biophysical journal

authors： Gallant EM,Curtis S,Pace SM,Dulhunty AF

更新日期：2001-04-01 00:00:00

abstract：:Skinned fibers of frog semitendinosus muscle could easily be stretched up to 8 mum or more in sarcomere length. Such extremely stretched fibers gave quite sharp optical diffraction patterns. The intensities of all observable diffraction lines were found to increase on application of electric field (10 similar to 100 V...

journal_title：Biophysical journal

authors： Umazume Y,Fujime S

更新日期：1975-02-01 00:00:00

abstract：:Subdenaturing concentrations of guanidine hydrochloride (GdnHCl) stabilize proteins. For ferrocytochrome c the stabilization is detected at subglobal level with no measured change in global stability. These deductions are made by comparing observed rates of thermally driven ferrocytochrome cHCO reactions with global u...

journal_title：Biophysical journal

authors： Kumar R,Prabhu NP,Yadaiah M,Bhuyan AK

更新日期：2004-10-01 00:00:00

abstract：:Electrically excitable channels were expressed in Chinese hamster ovary cells using a vaccinia virus vector system. In cells expressing rat brain IIA Na+ channels only, brief pulses (< 1 ms) of depolarizing current resulted in action potentials with a prolonged (0.5-3 s) depolarizing plateau; this plateau was caused b...

journal_title：Biophysical journal

authors： Hsu H,Huang E,Yang XC,Karschin A,Labarca C,Figl A,Ho B,Davidson N,Lester HA

更新日期：1993-09-01 00:00:00

abstract：:We report a simple method to obtain stable asymmetric giant unilamellar vesicles (GUVs). Fluorescence correlation spectroscopy was used to quantitatively characterize vesicle properties. After brain sphingomyelin (bSM) was exchanged into dioleoylphosphatidylcholine (DOPC) GUVs, lateral diffusion in the bSM-containing ...

journal_title：Biophysical journal

authors： Chiantia S,Schwille P,Klymchenko AS,London E

更新日期：2011-01-05 00:00:00

abstract：:High-efficiency electrofusion between cells of different sizes was achieved by application of fusing electric pulses to cells in centrifuged pellets. Larger target cells (Chinese hamster ovary or L1210 cells) were stacked among smaller human erythrocytes or erythrocyte ghosts by sequential centrifugation at 700 g to f...

journal_title：Biophysical journal

authors： Li LH,Hensen ML,Zhao YL,Hui SW

更新日期：1996-07-01 00:00:00

abstract：:The effect of uncharged, dipolar phloretin on anion and cation conductance through a black lipid membrane can be used to study its adsorption behavior. The adsorption of phloretin can be described by a Langmuir isotherm with weak dipole-dipole interaction. ...

journal_title：Biophysical journal

authors： de Levie R,Rangarajan SK,Seelig PF,Andersen OS

更新日期：1979-02-01 00:00:00

abstract：:The fluorescent Mg(2+) indicator furaptra (mag-fura-2) was introduced into single ventricular myocytes by incubation with its acetoxy-methyl ester form. The ratio of furaptra's fluorescence intensity at 382 and 350 nm was used to estimate the apparent cytoplasmic [Mg(2+)] ([Mg(2+)](i)). In Ca(2+)-free extracellular co...

journal_title：Biophysical journal

authors： Tursun P,Tashiro M,Konishi M

更新日期：2005-03-01 00:00:00

abstract：:Structure factor amplitudes and phases can be computed directly from electron cryomicroscopy images. Inherent aberrations of the electromagnetic lenses and other instrumental factors affect the structure factors, however, resulting in decreased accuracy in the determined three-dimensional reconstruction. In contrast, ...

journal_title：Biophysical journal

authors： Thuman-Commike PA,Tsuruta H,Greene B,Prevelige PE Jr,King J,Chiu W

更新日期：1999-04-01 00:00:00

abstract：:Disruption of the integrity of the plasma membrane by amyloidogenic proteins is linked to the pathogenesis of a number of common age-related diseases. Although accumulating evidence suggests that adverse environmental stressors such as unbalanced levels of metal ions may trigger amyloid-mediated membrane damage, many ...

journal_title：Biophysical journal

authors： Sciacca MF,Milardi D,Messina GM,Marletta G,Brender JR,Ramamoorthy A,La Rosa C

更新日期：2013-01-08 00:00:00

abstract：:The processes of cell growth and budding of the yeast cells Saccharomyces cerevisiae, which were gently immobilized on 3% agar and submerged in culture medium, were successfully imaged with an atomic force microscope for 6-7 h. Similar experiments on chemically fixed cells did not detect any appreciable change in thei...

journal_title：Biophysical journal

authors： Gad M,Ikai A

更新日期：1995-12-01 00:00:00

abstract：:Proteins are not rigid molecules, but exhibit internal motions on timescales ranging from femto- to milliseconds and beyond. In solution, proteins also experience global translational and rotational motions, sometimes on timescales comparable to those of the internal fluctuations. The possibility that internal and glo...

journal_title：Biophysical journal

authors： Hamaneh MB,Zhang L,Buck M

更新日期：2011-07-06 00:00:00

abstract：:We used the nonlinear Poisson-Boltzmann equation to calculate electrostatic potentials in the aqueous phase adjacent to model phospholipid bilayers containing mixtures of zwitterionic lipids (phosphatidylcholine) and acidic lipids (phosphatidylserine or phosphatidylglycerol). The aqueous phase (relative permittivity, ...

journal_title：Biophysical journal

authors： Peitzsch RM,Eisenberg M,Sharp KA,McLaughlin S

更新日期：1995-03-01 00:00:00

abstract：:It is widely assumed that the decay of fluorescence in photosynthetic systems can be described as a sum of exponential components and that the amplitude of each component is directly related to the absorption cross-section of the antenna pigments coupled to the fluorescing species. We present exact calculations of exc...

journal_title：Biophysical journal

authors： Jean JM,Chan CK,Fleming GR,Owens TG

更新日期：1989-12-01 00:00:00