Abstract:
:The dependence of phospholipid vesicle size on lipid composition is investigated by photon correlation spectroscopy. For each lipid composition prolonged ultracentrifugation was used to isolate a nearly uniform population of minimum-sized vesicles. The residual size variations in the samples were sufficient to cause polydispersity that made comparisons between samples difficult. Analyses of the data by the method of cumulants and by a method for approximating the particle size distributions directly are presented. The latter method made possible unambiguous comparisons that revealed small but systematic dependences of vesicle size on composition in vesicles containing mixtures of egg phosphatidylcholine and phosphatidylethanolamine, egg phosphatidylcholine and beef brain sphingomyelin, and in single lipid vesicles of egg phosphatidylcholine, dioleylphosphatidylcholine, and beef brain sphingomyelin. These size dependences are quantified within the resolution limits of the technique and their implications are discussed.
journal_name
Biophys Jjournal_title
Biophysical journalauthors
Goll J,Carlson FD,Barenholz Y,Litman BJ,Thompson TEdoi
10.1016/S0006-3495(82)84524-4subject
Has Abstractpub_date
1982-04-01 00:00:00pages
7-13issue
1eissn
0006-3495issn
1542-0086pii
S0006-3495(82)84524-4journal_volume
38pub_type
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