Evolution of the hemifused intermediate on the pathway to membrane fusion.

abstract：:The skeletal muscles of chickens, frogs, and fish have been reported to express two isoforms (alpha and beta) of the sarcoplasmic reticulum calcium release channel (ryanodine receptor or RYR), while mammals express only one. We have studied patterns of RYR isoform expression in skeletal muscles from a variety of fish,...

journal_title：Biophysical journal

authors： O'Brien J,Meissner G,Block BA

更新日期：1993-12-01 00:00:00

abstract：:Red blood cells (RBCs) have highly deformable viscoelastic membranes exhibiting complex rheological response and rich hydrodynamic behavior governed by special elastic and bending properties and by the external/internal fluid and membrane viscosities. We present a multiscale RBC model that is able to predict RBC mecha...

journal_title：Biophysical journal

authors： Fedosov DA,Caswell B,Karniadakis GE

更新日期：2010-05-19 00:00:00

abstract：:The role of cholesterol in the regulation of endosome motility was investigated by monitoring the intracellular trafficking of endocytosed folate receptors (FRs) labeled with fluorescent folate conjugates. Real-time fluorescence imaging of HeLa cells transfected with green fluorescent protein-tubulin revealed that FR-...

journal_title：Biophysical journal

authors： Chen H,Yang J,Low PS,Cheng JX

更新日期：2008-02-15 00:00:00

abstract：:Guanine-rich DNA repeat sequences located at the terminal ends of chromosomal DNA can fold in a sequence-dependent manner into G-quadruplex structures, notably the terminal 150-200 nucleotides at the 3' end, which occur as a single-stranded DNA overhang. The crystal structures of quadruplexes with two and four human t...

journal_title：Biophysical journal

authors： Haider S,Parkinson GN,Neidle S

更新日期：2008-07-01 00:00:00

abstract：:We have measured, using infrared spectroscopy, the hydrogen/deuterium exchange rates of the amide protons in the photosynthetic antenna of Rhodospirillum rubrum. These measurements were made not only on the intact protein in detergent solution but also on two dissociated forms (B820 and B777). We have, on the basis of...

journal_title：Biophysical journal

authors： Sturgis J,Robert B,Goormaghtigh E

更新日期：1998-02-01 00:00:00

abstract：:The diffusive motion of DNA-containing chromatin in live cells and isolated nuclei is investigated using a two-photon standing wave fluorescence photobleaching experiment with 100 nm spatial resolution. The chromatin is labeled using the minor groove binding dye Hoechst 33342. In live cells, the mean diffusion rate is...

journal_title：Biophysical journal

authors： Davis SK,Bardeen CJ

更新日期：2004-01-01 00:00:00

abstract：:A delay ( approximately 10 min) in the appearance of intracellular phage is caused by preincubating the infecting phage T4o(1) in proflavine, acridine orange, or ethidium, but not polyamines. No significant delay in attachment is observed. Apparently the presence of the dye is required inside the permeability barrier ...

journal_title：Biophysical journal

authors： Dusenbery DB,Uretz RB

更新日期：1972-08-01 00:00:00

abstract：:The Jun a 3 protein from mountain cedar (Juniperus ashei) pollen, a member of group 5 of the family of plant pathogenesis-related proteins (PR-proteins), reacts with serum IgE from patients with cedar hypersensitivity. We used the crystal structures of two other proteins of this group, thaumatin and an antifungal prot...

journal_title：Biophysical journal

authors： Soman KV,Midoro-Horiuti T,Ferreon JC,Goldblum RM,Brooks EG,Kurosky A,Braun W,Schein CH

更新日期：2000-09-01 00:00:00

abstract：:The influence of electrostatic multipole moments up to hexadecapole on the dynamics of photodissociated carbon monoxide (CO) in myoglobin is investigated. The CO electrostatic potential is expressed as an expansion into atomic multipole moments of increasing order up to octopole which are obtained from a distributed m...

journal_title：Biophysical journal

authors： Plattner N,Meuwly M

更新日期：2008-04-01 00:00:00

abstract：:Atomic force microscopy (AFM) has become an important tool for quantifying mechanical properties of biological materials ranging from single molecules to cells and tissues. Current AFM techniques for measuring elastic and viscoelastic properties of whole cells are based on indentation of cells firmly adhered to a subs...

journal_title：Biophysical journal

authors： Rosenbluth MJ,Lam WA,Fletcher DA

更新日期：2006-04-15 00:00:00

abstract：:Single-molecule studies allow the study of subtle activity differences due to local folding in proteins, but are time consuming and difficult because only a few molecules are observed in one experiment. We developed an assay where we can simultaneously measure the activity of hundreds of individual molecules. The assa...

journal_title：Biophysical journal

authors： Lee AI,Brody JP

更新日期：2005-06-01 00:00:00

abstract：:Endothelin-1 (ET-1) mediates physiological responses via endothelin A (ET(A)) and B (ET(B)) receptors, which may form homo- and heterodimers with unknown function. Here, we investigated ET-receptor dimerization using fluorescence resonance energy transfer (FRET) between receptors tagged with CFP (donor) and receptors ...

journal_title：Biophysical journal

authors： Evans NJ,Walker JW

更新日期：2008-07-01 00:00:00

abstract：:A one-dimensional version of the model recently proposed by Läuger (1988) to explain the closed-time distribution of ionic channels in cell membranes is solved analytically. While the probability density f(t) for closed-time lengths may show a well-defined exponential behavior at short times, a power-law decay is pred...

journal_title：Biophysical journal

authors： Condat CA,Jäckle J

更新日期：1989-05-01 00:00:00

abstract：:The biophysical characterization of nonfunctional protein aggregates at physiologically relevant temperatures is much needed to gain deeper insights into the kinetic and thermodynamic relationships between protein folding and misfolding. Dynamic and static laser light scattering have been employed for the detection an...

journal_title：Biophysical journal

authors： Chow C,Kurt N,Murphy RM,Cavagnero S

更新日期：2006-01-01 00:00:00

abstract：:The cell membrane having a transport system is inferred to be flexible when its function is being activated. For the brush border membrane vesicles prepared from rat small intestine, which have the co-transport system of Na+ and glucose, the membrane elasticity was measured as a function of the d-glucose concentration...

journal_title：Biophysical journal

authors： Miyamoto S,Maeda T,Fujime S

更新日期：1988-04-01 00:00:00

abstract：:In this paper we explore the properties of a mathematical model for the passive sodium permeability system of excitable membranes. This model is distinguished by the explicit inclusion of a rate constant which depends not on instantaneous voltage, but on rate of voltage change. Actually, the model is a rather modest m...

journal_title：Biophysical journal

authors： Jakobsson E,Scudiero C

更新日期：1975-06-01 00:00:00

abstract：:We describe simulations of absorption difference spectra in strongly coupled photosynthetic antennas. In the presence of large resonance couplings, distinctive features arise from excited-state absorption transitions between one- and two-exciton levels. We first outline the theory for the heterodimer and for the gener...

journal_title：Biophysical journal

authors： Buck DR,Savikhin S,Struve WS

更新日期：1997-01-01 00:00:00

abstract：:The stability of the structure of double-stranded DNA in the salt-free solution is discussed on the basis of the polyelectrolyte theory. Assuming that DNA is an infinitely long rod, and the formation of double strands is divided into combining process and folding process, the free energy changes required in these proc...

journal_title：Biophysical journal

authors： OHNISHI T

更新日期：1963-11-01 00:00:00

abstract：:Wiener kernels have been measured for the light growth response of a number of mutants of Phycomyces which show abnormal phototropism (mad mutants). Representative mutants were chosen from the six complementation groups (madA to madF) associated with the light response pathway. One group, madA, associated with the inp...

journal_title：Biophysical journal

authors： Lipson ED

更新日期：1975-10-01 00:00:00

abstract：:In this study we describe the expression and function of the two rat type-1 inositol 1,4,5-trisphosphate receptor (InsP3R) ligand binding domain splice variants (SI+/-/SII+). Receptor protein from COS-1 cells transfected with the type-1 InsP3R expression plasmids (pInsP3R-T1, pInsP3R-T1ALT) or control DNA were incorpo...

journal_title：Biophysical journal

authors： Ramos-Franco J,Caenepeel S,Fill M,Mignery G

更新日期：1998-12-01 00:00:00

abstract：:Chimeras consisting of the homologous skeletal dihydropyridine receptor (DHPR) beta1a subunit and the heterologous cardiac/brain beta2a subunit were used to determine which regions of beta1a were responsible for the skeletal-type excitation-contraction (EC) coupling phenotype. Chimeras were transiently transfected in ...

journal_title：Biophysical journal

authors： Sheridan DC,Cheng W,Carbonneau L,Ahern CA,Coronado R

更新日期：2004-08-01 00:00:00

abstract：:Recent experimental studies suggest that lactate dehydrogenase (LDH) binds its substrate via the formation of a LDH/NADH.substrate encounter complex through a select-fit mechanism, whereby only a minority population of LDH/NADH is binding-competent. In this study, we perform molecular dynamics calculations to explore ...

journal_title：Biophysical journal

authors： Pineda JR,Callender R,Schwartz SD

更新日期：2007-09-01 00:00:00

abstract：:The aquaglyceroporin GlpF is a transmembrane channel of Escherichia coli that facilitates the uptake of glycerol by the cell. Its high glycerol uptake rate is crucial for the cell to survive in very low glycerol concentrations. Although GlpF allows both influx and outflux of glycerol, its structure, similar to the str...

journal_title：Biophysical journal

authors： Lu D,Grayson P,Schulten K

更新日期：2003-11-01 00:00:00

abstract：:The membrane protein bacteriorhodopsin (BR) can be kept soluble in its native state for months in the absence of detergent by amphipol (APol) A8-35, an amphiphilic polymer. After an actinic flash, A8-35-complexed BR undergoes a complete photocycle, with kinetics intermediate between that in detergent solution and that...

journal_title：Biophysical journal

authors： Gohon Y,Dahmane T,Ruigrok RW,Schuck P,Charvolin D,Rappaport F,Timmins P,Engelman DM,Tribet C,Popot JL,Ebel C

更新日期：2008-05-01 00:00:00

abstract：:The effects of internally and externally applied sulfhydryl reagents on the bioelectric properties of the giant axon of the squid Loligo pealeii and Dosidicus gigas were studied. Cysteine-HCl (400 mM, pH 7.3) was used to remove axoplasm from the perfusion channel. Oxidizing agents (1 to 60 mM) tended to increase the d...

journal_title：Biophysical journal

authors： Huneeus-Cox F,Fernandez HL,Smith BH

更新日期：1966-09-01 00:00:00

abstract：:Fluorescence recovery after photobleaching (FRAP) has been used previously to investigate the kinetics of binding to biological surfaces. The present study adapts and further develops this technique for the quantification of mass transport and reaction parameters in bulk media. The technique's ability to obtain the bu...

journal_title：Biophysical journal

authors： Kaufman EN,Jain RK

更新日期：1991-09-01 00:00:00

abstract：:Phytochromes sense red/far-red light and control many biological processes in plants, fungi, and bacteria. Although the crystal structures of dark- and light-adapted states have been determined, the molecular mechanisms underlying photoactivation remain elusive. Here, we demonstrate that the conserved tongue region of...

journal_title：Biophysical journal

authors： Gustavsson E,Isaksson L,Persson C,Mayzel M,Brath U,Vrhovac L,Ihalainen JA,Karlsson BG,Orekhov V,Westenhoff S

更新日期：2020-01-21 00:00:00

abstract：:The effects of intracellular magnesium ions and extracellular calcium and magnesium ions on the delayed rectifier potassium ion channel, IK, were investigated from intracellularly perfused squid giant axons. Cao+2 and Mgo+2 both blocked IK in a voltage-independent manner with a KD of approximately 100 and 500 mM, resp...

journal_title：Biophysical journal

authors： Clay JR

更新日期：1995-11-01 00:00:00

abstract：:Steady-state and time-resolved fluorescence spectroscopy was used to follow the local and global changes in structure and dynamics during chemical and thermal denaturation of unlabeled human serum albumin (HSA) and HSA with an acrylodan moiety bound to Cys34. Acrylodan fluorescence was monitored to obtain information ...

journal_title：Biophysical journal

authors： Flora K,Brennan JD,Baker GA,Doody MA,Bright FV

更新日期：1998-08-01 00:00:00

abstract：:We report molecular dynamics simulations in the explicit membrane environment of a small membrane-embedded protein, sarcolipin, which regulates the sarcoplasmic reticulum Ca-ATPase activity in both cardiac and skeletal muscle. In its monomeric form, we found that sarcolipin adopts a helical conformation, with a comput...

journal_title：Biophysical journal

authors： Shi L,Cembran A,Gao J,Veglia G

更新日期：2009-05-06 00:00:00