Polarized infrared spectroscopy of oriented purple membrane.

abstract：:The temporal dynamics of the concentrations of several proteins are tightly regulated, particularly for critical nodes in biological networks such as transcription factors. An important mechanism to control transcription factor levels is through autoregulatory feedback loops where the protein can bind its own promoter...

journal_title：Biophysical journal

authors： Murugan R,Kreiman G

更新日期：2011-09-21 00:00:00

abstract：:Fluorescent labels are often used in bioassays as a means to detect and characterize ligand-receptor binding. This is due in part to the inherently high sensitivity of fluorescence-based technology and the relative accessibility of the technique. There is often little concern raised as to whether or not the fluorescen...

journal_title：Biophysical journal

authors： Swift JL,Cramb DT

更新日期：2008-07-01 00:00:00

abstract：:Some new approaches to the kinetic study of the reduction of cytochrome aa3 by cytochrome c are presented. The primary innovations are the use of a spectrometer which can acquire multiwavelength data as fast as every 10 microseconds, and the application of a variety of analytical methods which can utilize simultaneous...

journal_title：Biophysical journal

authors： Hendler RW,Bose SK,Shrager RI

更新日期：1993-09-01 00:00:00

abstract：:We present a truly quantitative fluorescence recovery after photobleaching (FRAP) model for use with the confocal laser scanning microscope based on the photobleaching of a long line segment. The line FRAP method is developed to complement the disk FRAP method reported before. Although being more subject to the influe...

journal_title：Biophysical journal

authors： Braeckmans K,Remaut K,Vandenbroucke RE,Lucas B,De Smedt SC,Demeester J

更新日期：2007-03-15 00:00:00

abstract：:In skeletal muscle, excitation-contraction coupling involves the activation of dihydropyridine receptors (DHPR) and type-1 ryanodine receptors (RyR1) to produce depolarization-dependent sarcoplasmic reticulum Ca²⁺ release via orthograde signaling. Another form of DHPR-RyR1 communication is retrograde signaling, in whi...

journal_title：Biophysical journal

authors： Olojo RO,Hernández-Ochoa EO,Ikemoto N,Schneider MF

更新日期：2011-05-18 00:00:00

abstract：:A simple method of generating electric field-induced concentration gradients in planar supported bilayers has been developed. Gradients of charged, fluorescently labeled probes were visualized by epifluorescence microscopy and could be observed at field strengths as low as 1 V/cm. Steady-state concentration gradients ...

journal_title：Biophysical journal

authors： Groves JT,Boxer SG

更新日期：1995-11-01 00:00:00

abstract：:The kinetics of aggregation of alpha-synuclein are usually studied by turbidity or Thio-T fluorescence. Here we follow the disappearance of monomers and the formation of early oligomers using fluorescence correlation spectroscopy. Alexa488-labeled A140C-synuclein was used as a fluorescent probe in trace amounts in the...

journal_title：Biophysical journal

authors： Nath S,Meuvis J,Hendrix J,Carl SA,Engelborghs Y

更新日期：2010-04-07 00:00:00

abstract：:Nonphotochemical quenching is the protective mechanism against overexcitation of photosystem II, triggered by excess ΔpH in photosynthetic membranes. The light-harvesting complexes (LHCs), the de-epoxidation of violaxanthin to zeaxanthin, and the photosystem II subunit S (PsbS) work in synergy for an optimized multile...

journal_title：Biophysical journal

authors： Daskalakis V,Papadatos S

更新日期：2017-12-05 00:00:00

abstract：:Förster resonance energy transfer (FRET) has become an important tool for analyzing different aspects of interactions among biological macromolecules in their native environments. FRET analysis has also been successfully applied to study the spatiotemporal regulation of various cellular processes using genetically enc...

journal_title：Biophysical journal

authors： Zeug A,Woehler A,Neher E,Ponimaskin EG

更新日期：2012-11-07 00:00:00

abstract：:Slow stretch ramps (velocity: 0.17 fiber lengths s-1) were imposed during fused tetanic contractions of intact muscle fibers of the frog (1.4-3.0 degrees C; sarcomere length: 2.12-2.21 microns). Instantaneous force-extension relations were derived both under isometric conditions and during slow stretch by applying fas...

journal_title：Biophysical journal

authors： Månsson A

更新日期：1989-08-01 00:00:00

abstract：:Thermotropic phase transitions in single planar bilayers of glycerol mono-oleate have been investigated using quasi-elastic light scattering from thermally excited membrane fluctuations. In certain cases both spectroscopic and intensity information were derived from the observations. For solvent-free bilayers transiti...

journal_title：Biophysical journal

authors： Crawford GE,Earnshaw JC

更新日期：1986-04-01 00:00:00

abstract：:A detailed experimental description of molecular dynamics requires an accurate description of the global correlation time. For the gramicidin cation-selective channel the local dynamics of the polypeptide backbone are thought to play a very significant role in the functional process of this channel which occurs on the...

journal_title：Biophysical journal

authors： Lee KC,Hu W,Cross TA

更新日期：1993-09-01 00:00:00

abstract：:It is known that the action of general anesthetics is proportional to their partition coefficient in lipid membranes (Meyer-Overton rule). This solubility is, however, directly related to the depression of the temperature of the melting transition found close to body temperature in biomembranes. We propose a thermodyn...

journal_title：Biophysical journal

authors： Heimburg T,Jackson AD

更新日期：2007-05-01 00:00:00

abstract：:Equations describing the movement of sugars during induced uphill transport were derived on the assumption of a simple carrier transport mechanism and subjected to experimental verification. Since there was good agreement between the experimental points and the theoretical curves, no changes in the original postulates...

journal_title：Biophysical journal

authors： Miller DM

更新日期：1965-07-01 00:00:00

abstract：:The closed-state crystal structure of prokaryotic inward rectifier, KirBac1.1, has implicated four inner helical phenylalanines near the cytoplasmic side as a possible locus of the channel gate. In the present study, we investigate whether this structural feature corresponds to the physiological pH gate of the renal i...

journal_title：Biophysical journal

authors： Sackin H,Nanazashvili M,Palmer LG,Krambis M,Walters DE

更新日期：2005-04-01 00:00:00

abstract：:Carbonic anhydrase (CA) is a thoroughly studied enzyme. Its primary role is the rapid interconversion of carbon dioxide and bicarbonate in the cells, where carbon dioxide is produced, and in the lungs, where it is released from the blood. At the same time, it regulates pH homeostasis. The inhibitory function of sulfon...

journal_title：Biophysical journal

authors： Jonsson BH,Liljas A

更新日期：2020-10-06 00:00:00

abstract：:The glucose transporters (GLUT/SLC2A) are members of the major facilitator superfamily. Here, we generated a three-dimensional model for Glut1 using a two-step strategy: 1), GlpT structure as an initial homology template and 2), evolutionary homology using glucose-6-phosphate translocase as a template. The resulting s...

journal_title：Biophysical journal

authors： Salas-Burgos A,Iserovich P,Zuniga F,Vera JC,Fischbarg J

更新日期：2004-11-01 00:00:00

abstract：:The microenvironment between the plasma membrane and the near-membrane sarcoplasmic reticulum (SR) may play an important role in Ca(2+) regulation in smooth muscle cells. We used a three-dimensional mathematical model of Ca(2+) diffusion and regulation and experimental measurements of SR Ca(2+) uptake and the distribu...

journal_title：Biophysical journal

authors： Bazzazi H,Kargacin ME,Kargacin GJ

更新日期：2003-09-01 00:00:00

abstract：:Halobacterium halobium contains at least three retinal-containing pigments: bacteriorhodopsin, halorhodopsin, and a third rhodopsin-like pigment (tR) absorbing at approximately 590 nm, tR590. Illumination of tR590 gives rise to a very long-lived blue absorbing photoproduct, tR370. Using high-performance liquid chromat...

journal_title：Biophysical journal

authors： Tsuda M,Nelson B,Chang CH,Govindjee R,Ebrey TG

更新日期：1985-05-01 00:00:00

abstract：:Recent work has introduced a new fluorescent voltage sensor, ASAP1, which can monitor rapid trains of action potentials in cultured neurons. This indicator is based on the Gallus gallus voltage-sensitive phosphatase with the phosphatase domain removed and a circularly permuted GFP placed in the S3-S4 linker. However, ...

journal_title：Biophysical journal

authors： Lee EEL,Bezanilla F

更新日期：2017-11-21 00:00:00

abstract：:Cholesterol, due to its condensing effect, is considered an important regulator of membrane thickness. Other sterols, due to their structural similarities to cholesterol, are often assumed to have a universal effect on membrane properties similar to the condensing effect of cholesterol, albeit possibly to different de...

journal_title：Biophysical journal

authors： Hung WC,Lee MT,Chung H,Sun YT,Chen H,Charron NE,Huang HW

更新日期：2016-05-10 00:00:00

abstract：:The pulling of micropipette electrodes from glass tubing has been treated as a problem of viscous flow coupled with the Newtonian dynamics of the pulling apparatus. Analytical solutions are given from which the taper profile, tip diameter, and pulling time can be obtained. The physical principles of operation of micro...

journal_title：Biophysical journal

authors： Purves RD

更新日期：1980-03-01 00:00:00

abstract：:The selective permeability to monovalent metal cations, as well as the relationship between cation permeation and gating kinetics, was investigated for native tetrodotoxin-insensitive Na-channels in guinea pig ventricular myocytes using the whole-cell patch clamp technique. By the measurement of inward unidirectional ...

journal_title：Biophysical journal

authors： Kurata Y,Sato R,Hisatome I,Imanishi S

更新日期：1999-10-01 00:00:00

abstract：:Fluorescence recovery after photobleaching (FRAP) has been used previously to investigate the kinetics of binding to biological surfaces. The present study adapts and further develops this technique for the quantification of mass transport and reaction parameters in bulk media. The technique's ability to obtain the bu...

journal_title：Biophysical journal

authors： Kaufman EN,Jain RK

更新日期：1991-09-01 00:00:00

abstract：:Dispersed pump-dump-probe spectroscopy has the ability to characterize and identify the underlying ultrafast dynamical processes in complicated chemical and biological systems. This technique builds on traditional pump-probe techniques by exploring both ground- and excited-state dynamics and characterizing the connect...

journal_title：Biophysical journal

authors： Larsen DS,Vengris M,van Stokkum IH,van der Horst MA,de Weerd FL,Hellingwerf KJ,van Grondelle R

更新日期：2004-04-01 00:00:00

abstract：:Voltage-sensitive fluorescent dyes are commonly used to measure cardiac electrical activity. Recent studies indicate, however, that optical action potentials (OAPs) recorded from the myocardial surface originate from a widely distributed volume beneath the surface and may contain useful information regarding intramura...

journal_title：Biophysical journal

authors： Hyatt CJ,Mironov SF,Wellner M,Berenfeld O,Popp AK,Weitz DA,Jalife J,Pertsov AM

更新日期：2003-10-01 00:00:00

abstract：:Enterococcus hirae V1-ATPase is a molecular motor composed of the A3B3 hexamer ring and the central stalk. In association with ATP hydrolysis, three catalytic AB pairs in the A3B3 ring undergo conformational changes, which lead to a 120° rotation of the central stalk. To understand how the conformational changes of th...

journal_title：Biophysical journal

authors： Isaka Y,Ekimoto T,Kokabu Y,Yamato I,Murata T,Ikeguchi M

更新日期：2017-03-14 00:00:00

abstract：:The mechanical properties of cross-linked microtubule bundles were measured from outer pillar cells isolated from the mammalian inner ear. Measurements were made using a three-point bending test and were incorporated into a mathematical model designed to distinguish between the stiffness contributions from microtubule...

journal_title：Biophysical journal

authors： Tolomeo JA,Holley MC

更新日期：1997-10-01 00:00:00

abstract：:Uncovering mechanisms that control the dynamics of microtubules is fundamental for our understanding of multiple cellular processes such as chromosome separation and cell motility. Building on previous theoretical work on the dynamic instability of microtubules, we propose here a stochastic model that includes all rel...

journal_title：Biophysical journal

authors： Padinhateeri R,Kolomeisky AB,Lacoste D

更新日期：2012-03-21 00:00:00

abstract：:The thylakoid membrane has a unique lipid composition, consisting mostly of galactolipids. These thylakoid lipids have important roles in photosynthesis. Here, we investigate to what extent these lipids bind specifically to the Photosystem II complex. To this end, we performed coarse-grain MD simulations of the Photos...

journal_title：Biophysical journal

authors： Van Eerden FJ,Melo MN,Frederix PWJM,Marrink SJ

更新日期：2017-12-19 00:00:00