Structural relaxation and nonexponential kinetics of CO-binding to horse myoglobin. Multiple flash photolysis experiments.

abstract：:Viral fusion peptides are short N-terminal regions of type-1 viral fusion proteins that are critical for virus entry. Although the importance of viral fusion peptides in virus-cell membrane fusion is established, little is known about how they function. We report the effects of wild-type (WT) hemagglutinin (HA) fusion...

journal_title：Biophysical journal

authors： Chakraborty H,Tarafdar PK,Klapper DG,Lentz BR

更新日期：2013-12-03 00:00:00

abstract：:Cell adhesion provides not only physical linkage but also communication between the cell and its environment. As such, it is important to many cellular functions. Recently, the probability distribution of forming a low number of specific adhesive bonds in a short-duration contact has been described (Chesla et al., Bio...

journal_title：Biophysical journal

authors： Zhu C,Williams TE

更新日期：2000-10-01 00:00:00

abstract：:Adaptation of the attractant response in Escherichia coli is attributable to the methylation of its transmembrane chemotactic receptors by the methyltransferase CheR. This protein contains two binding domains, one for the sites of methylation themselves and the other for a flexible tether at the C terminus of the rece...

journal_title：Biophysical journal

authors： Levin MD,Shimizu TS,Bray D

更新日期：2002-04-01 00:00:00

abstract：:We report the crystal structure of a bromide-bound form of the D85S mutant of bacteriorhodopsin, bR(D85S), a protein that uses light energy rather than ATP to pump halide ions across the cell membrane. Comparison of the structure of the halide-bound and halide-free states reveals that both displacements of individual ...

journal_title：Biophysical journal

authors： Facciotti MT,Cheung VS,Nguyen D,Rouhani S,Glaeser RM

更新日期：2003-07-01 00:00:00

abstract：:A model for the action of vinblastine (VLB) on cells multiplying exponentially in vivo with a generation time, T(G), has been derived. It is based on the assumption that cells attempting to pass through mitosis in the presence of VLB lose their proliferative capacity and that this lethal effect occurs only when the ce...

journal_title：Biophysical journal

authors： Valeriote FA,Bruce WR,Meeker BE

更新日期：1966-03-01 00:00:00

abstract：:We have used magnetic relaxation dispersion to study bovine pancreatic trypsin inhibitor (BPTI) self-association as a function of pH, salt type and concentration, and temperature. The magnetic relaxation dispersion method sensitively detects stable oligomers without being affected by other interactions. We find that B...

journal_title：Biophysical journal

authors： Gottschalk M,Venu K,Halle B

更新日期：2003-06-01 00:00:00

abstract：:More and more researchers are interested in imaging living (Henderson, 1994) or fixed cells in their natural environment using the atomic force microscope (AFM). However, the AFM tip interacts strongly with the sample, and its z range freedom is limited to a few micrometers. This means that the cells to be imaged have...

journal_title：Biophysical journal

authors： Kasas S,Ikai A

更新日期：1995-05-01 00:00:00

abstract：:In a relaxed muscle fiber at low ionic strength, the cross-bridges may well be in states comparable to the one that precedes the cross-bridge power stroke (Schoenberg, M. 1988. Adv. Exp. Med. Biol. 226:189-202). Using electron paramagnetic resonance (EPR) and (saturation transfer) electron paramagnetic resonance (ST-E...

journal_title：Biophysical journal

authors： Fajer PG,Fajer EA,Schoenberg M,Thomas DD

更新日期：1991-09-01 00:00:00

abstract：:A general analytical description of the equilibrium and reaction kinetics of DNA multiplex hybridization has been developed. In this approach, multiplex hybridization is considered to be a competitive multichannel reaction process: a system wherein many species can react both specifically and nonspecifically with one ...

journal_title：Biophysical journal

authors： Horne MT,Fish DJ,Benight AS

更新日期：2006-12-01 00:00:00

abstract：:We previously used an orthogonal high-throughput screen to select peptides that spontaneously cross synthetic lipid bilayers without bilayer disruption. Many of the 12-residue spontaneous membrane translocating peptides (SMTPs) selected from the library contained a 5-residue consensus motif, LRLLR in positions 5-9. We...

journal_title：Biophysical journal

authors： Fuselier T,Wimley WC

更新日期：2017-08-22 00:00:00

abstract：:We develop a model of tyrosine phosphorylation and activation of the T-cell receptor (TCR) by localization to regions of close membrane-membrane proximity (close contact) that physically exclude tyrosine phosphatases such as CD45. Phosphatase exclusion generates regions of low phosphatase and high kinase activity and ...

journal_title：Biophysical journal

authors： Burroughs NJ,Lazic Z,van der Merwe PA

更新日期：2006-09-01 00:00:00

abstract：:The problem of the desorption of a lipid molecule from a lipid vesicle (donor) and its incorporation into another vesicle (acceptor) at high acceptor concentrations, which has been investigated experimentally (Jones, J. D. and Thompson, T. E., 1990. Biochemistry, 29:1593-1600), is analyzed here from a theoretical poin...

journal_title：Biophysical journal

authors： Almeida PF

更新日期：1999-04-01 00:00:00

abstract：:Light adaptation in cones was characterized by measuring the changes in temporal frequency responses to sinusoidal modulation of light around various mean levels spanning a range of four log units. We have shown previously that some aspects of cone adaptation behavior can be accounted for by a biochemical kinetic mode...

journal_title：Biophysical journal

authors： Tranchina D,Sneyd J,Cadenas ID

更新日期：1991-07-01 00:00:00

abstract：:We present an analysis of physical chemical constraints on the accuracy of DNA micro-arrays under equilibrium and nonequilibrium conditions. At the beginning of the article we describe an algorithm for choosing a probe set with high specificity for targeted genes under equilibrium conditions. The algorithm as well as ...

journal_title：Biophysical journal

authors： Bhanot G,Louzoun Y,Zhu J,DeLisi C

更新日期：2003-01-01 00:00:00

abstract：:High resolution dynamics and structural information has been resolved from 2H solid-state NMR spectra of the Val-1 side-chain of the gramicidin channel in a lipid bilayer. Both powder pattern lineshapes and spectra from uniformly aligned samples of gramicidin in lipid bilayers have been analyzed to achieve a fully con...

journal_title：Biophysical journal

authors： Lee KC,Cross TA

更新日期：1994-05-01 00:00:00

abstract：:Bathorhodopsins were prepared by partially (10--15%) photoconverting bovine rhodopsin (11-cis chromophore) or isorhodopsin I (9-cis chromophore) at 77 degrees K; care was taken to avoid establishing photostationary states. The absorption spectra calculated for the bathorhodopsins derived from the two parent pigments a...

journal_title：Biophysical journal

authors： Mao B,Ebrey TG,Crouch R

更新日期：1980-02-01 00:00:00

abstract：:The interactions between vitamin-A and beta-lactoglobulin have been investigated. We have found that two different complexes can be formed: one involving vitamin-A, and one involving a derivative of vitamin-A that most probably has a retro-beta-ionylidene structure. Room temperature absorption spectra for these comple...

journal_title：Biophysical journal

authors： Hemley R,Kohler BE,Siviski P

更新日期：1979-12-01 00:00:00

abstract：:Genomics is a sequence-based informatics science and a three-dimensional-structure-based material science. However, in practice, most genomics researchers utilize sequence-based informatics approaches or three-dimensional-structure-based material science techniques, not both. This division is, at least in part, the re...

journal_title：Biophysical journal

authors： Li Z,Sun R,Bishop TC

更新日期：2020-05-05 00:00:00

abstract：:We have measured, using infrared spectroscopy, the hydrogen/deuterium exchange rates of the amide protons in the photosynthetic antenna of Rhodospirillum rubrum. These measurements were made not only on the intact protein in detergent solution but also on two dissociated forms (B820 and B777). We have, on the basis of...

journal_title：Biophysical journal

authors： Sturgis J,Robert B,Goormaghtigh E

更新日期：1998-02-01 00:00:00

abstract：:Muscles have evolved to power a wide variety of movements. A protein component critical to varying power generation is the myosin isoform present in the muscle. However, how functional variation in muscle arises from myosin structure is not well understood. We studied the influence of the converter, a myosin structura...

journal_title：Biophysical journal

authors： Glasheen BM,Ramanath S,Patel M,Sheppard D,Puthawala JT,Riley LA,Swank DM

更新日期：2018-03-13 00:00:00

abstract：:The p7 protein from hepatitis C virus is critical for the assembly and secretion of infectious virus, making it an attractive drug target. It is thought to be a viroporin with a demonstrated ion channel activity when reconstituted into planar lipid bilayers. Electron microscopy experiments suggest that p7 oligomers co...

journal_title：Biophysical journal

authors： Chew CF,Vijayan R,Chang J,Zitzmann N,Biggin PC

更新日期：2009-01-01 00:00:00

abstract：:Chemotactic effects of dissolved oxygen on motions of Escherichia coli in a motility buffer solution have been studied by measurements of quasielastic light scattering. Under conditions where the bacteria form a sharp band in an oxygen concentrations gradient created by their metabolism, components of motions along th...

journal_title：Biophysical journal

authors： Wang PC,Chen SH

更新日期：1981-10-01 00:00:00

abstract：:It has been accepted that the force produced by a skeletal muscle myofibril depends on its cross-sectional area but not on the number of active sarcomeres because they are arranged in series. However, a previous study performed by our group showed that blocking actomyosin interactions within an activated myofibril and...

journal_title：Biophysical journal

authors： Mendoza AC,Rassier DE

更新日期：2020-04-21 00:00:00

abstract：:The kinetics of porin incorporation into black lipid membranes (BLM) made of phosphatidylinositol (PI) or oxidized cholesterol (Ox Ch) were studied by means of alternating current; the set-up was able to acquire resistance and capacitance simultaneously by means of a mixed double-frequency approach at 1 Hz and 1 KHz, ...

journal_title：Biophysical journal

authors： Gallucci E,Micelli S,Monticelli G

更新日期：1996-08-01 00:00:00

abstract：:A 10-ns molecular dynamics simulation of mouse acetylcholinesterase was analyzed, with special attention paid to the fluctuation in the width of the gorge and opening events of the back door. The trajectory was first verified to ensure its stability. We defined the gorge proper radius as the measure for the extent of ...

journal_title：Biophysical journal

authors： Tai K,Shen T,Börjesson U,Philippopoulos M,McCammon JA

更新日期：2001-08-01 00:00:00

abstract：:Sodium perchlorate salt (NaClO(4)) is commonly used as an internal intensity standard in ultraviolet resonance Raman (UVRR) spectroscopy experiments. It is well known that NaClO(4) can have profound effects on peptide stability. The impact of NaClO(4) on protein stability in UVRR experiments has not yet been fully inv...

journal_title：Biophysical journal

authors： Asciutto EK,General IJ,Xiong K,Asher SA,Madura JD

更新日期：2010-01-20 00:00:00

abstract：:The rotation of human red blood cells (RBC) as they flow in the shear field established by a Coulter type orifice is modeled. This model, based on hydrodynamics of ellipsoid rotation in laminar creeping flow, is used to calculate the probability of the cells entering the orifice with a specific orientation. The electr...

journal_title：Biophysical journal

authors： Breitmeyer MO,Lightfoot EN,Dennis WH

更新日期：1971-02-01 00:00:00

abstract：:Metallic nanoparticles have opened the possibility of imaging, tracking, and manipulating biological samples without time limitations. Their low photoluminescence quantum yield, however, makes them hard to detect under high background conditions. In this study we show that it is possible to image gold nanorods by dete...

journal_title：Biophysical journal

authors： Carattino A,Keizer VIP,Schaaf MJM,Orrit M

更新日期：2016-12-06 00:00:00

abstract：:Information processing and decision-making is based upon logic operations, which in cellular networks has been well characterized at the level of transcription. In recent years, however, both experimentalists and theorists have begun to appreciate that cellular decision-making can also be performed at the level of a s...

journal_title：Biophysical journal

authors： de Ronde W,Rein ten Wolde P,Mugler A

更新日期：2012-09-05 00:00:00

abstract：:Effects of alkali cations on the nuclear magnetic resonance intensity of 23Na were studied in rat liver homogenate. The loss in the resonance intensity of 23Na in the homogenate was able to be divided into two components, one of which is abolished by the addition of Cs+ ("Cs-sensitive component"), the other being inse...

journal_title：Biophysical journal

authors： Monoi H

更新日期：1976-12-01 00:00:00