Dynamic imaging of lateral diffusion by electron spin resonance and study of rotational dynamics in model membranes. Effect of cholesterol.

abstract：:The centromeric histone H3 variant centromeric protein A (CENP-A), whose sequence is the least conserved among all histone variants, is responsible for specifying the location of the centromere. Here, we present a comprehensive study of CENP-A nucleosome arrays by cryo-electron tomography. We see that CENP-A arrays ha...

journal_title：Biophysical journal

authors： Geiss CP,Keramisanou D,Sekulic N,Scheffer MP,Black BE,Frangakis AS

更新日期：2014-02-18 00:00:00

abstract：:Microscopic calcium (Ca2+) events (such as Ca2+ sparks) are an important area for study, as they help clarify the mechanism(s) underlying intracellular signaling. In the heart, Ca2+ sparks occur as a result of Ca2+ release from the sarcoendoplasmic reticulum, via ryanodine receptor channels. Measurement of Ca2+ spark ...

journal_title：Biophysical journal

authors： Kong CH,Soeller C,Cannell MB

更新日期：2008-12-15 00:00:00

abstract：:The outer membrane (OM) of Gram-negative bacteria is composed of lipopolysaccharide (LPS) in the outer leaflet and phospholipids in the inner leaflet. The outer membrane protein H (OprH) of Pseudomonas aeruginosa provides an increased stability to the OMs by directly interacting with LPS. Here we report the influence ...

journal_title：Biophysical journal

authors： Lee J,Patel DS,Kucharska I,Tamm LK,Im W

更新日期：2017-01-24 00:00:00

abstract：:Cell proliferation and contact inhibition play a major role in maintaining epithelial cell homeostasis. Prior experiments have shown that externally applied forces, such as stretch, result in increased proliferation in an E-cadherin force-dependent manner. In this study, the spatial regulation of cell proliferation in...

journal_title：Biophysical journal

authors： Mohan A,Schlue KT,Kniffin AF,Mayer CR,Duke AA,Narayanan V,Arsenovic PT,Bathula K,Danielsson BE,Dumbali SP,Maruthamuthu V,Conway DE

更新日期：2018-09-04 00:00:00

abstract：:Certain environmental parameters are accessible to cells only indirectly and require an encoding step for cells to retrieve the relevant information. A prominent example is the phenomenon of quorum sensing by microorganisms, where information about cell density is encoded by means of secreted signaling molecules. The ...

journal_title：Biophysical journal

authors： Drees B,Reiger M,Jung K,Bischofs IB

更新日期：2014-07-01 00:00:00

abstract：:The processes of cell growth and budding of the yeast cells Saccharomyces cerevisiae, which were gently immobilized on 3% agar and submerged in culture medium, were successfully imaged with an atomic force microscope for 6-7 h. Similar experiments on chemically fixed cells did not detect any appreciable change in thei...

journal_title：Biophysical journal

authors： Gad M,Ikai A

更新日期：1995-12-01 00:00:00

abstract：:Communication-deficient cells (the SKHep1 cell line) were stably transfected with a plasmid containing cDNA which encodes the major gap junction protein of rat liver, connexin32. Application of the dual whole-cell voltage clamp technique with patch electrodes to pairs of transfected SKHep1 cells revealed strong sensit...

journal_title：Biophysical journal

authors： Moreno AP,Eghbali B,Spray DC

更新日期：1991-11-01 00:00:00

abstract：:Low-power continuous-wave laser radiation is used to form a very stable microbubble at the end of a specially etched and metalized optical fiber probe. We demonstrate that the microbubble, which is firmly attached to the fiber probe, can be used to benignly trap and manipulate living swine sperm cells as well as human...

journal_title：Biophysical journal

authors： Diop M,Taylor R

更新日期：2006-05-15 00:00:00

abstract：:The mechanism of the "ground permeability" of the human erythrocyte membrane for K+ and Na+ was investigated with respect to a possible involvement of a previously unidentified specific transport pathway, because earlier studies showed that it cannot be explained on the basis of simple electrodiffusion. In particular,...

journal_title：Biophysical journal

authors： Richter S,Hamann J,Kummerow D,Bernhardt I

更新日期：1997-08-01 00:00:00

abstract：:Cord factor (trehalose 6,6'-dimycolate, CF) is a glycolipid located in the outer mycobacterial cell wall that is implicated in the pathogenesis of mycobacteria. Furthermore, CF is a convenient model for studying mycolic acid residues, the major lipid constituents of the mycobacterial cell wall that are believed to for...

journal_title：Biophysical journal

authors： Almog R,Mannella CA

更新日期：1996-12-01 00:00:00

abstract：:Ras is a membrane-anchored signaling protein that serves as a hub for many signaling pathways and also plays a prominent role in cancer. The intrinsic behavior of Ras on the membrane has captivated the biophysics community in recent years, especially the possibility that it may form dimers. In this article, we describ...

journal_title：Biophysical journal

authors： Chung JK,Lee YK,Denson JP,Gillette WK,Alvarez S,Stephen AG,Groves JT

更新日期：2018-01-09 00:00:00

abstract：:Diffusional encounters between a dumbbell model of a cleft enzyme and a dumbbell model of an elongated ligand are simulated by Brownian dynamics. The simulations take into account electrostatic and hydrodynamic interactions between the molecules. It is shown that the primary effect of inclusion of hydrodynamic interac...

journal_title：Biophysical journal

authors： Antosiewicz J,McCammon JA

更新日期：1995-07-01 00:00:00

abstract：:Negatively charged phospholipids are an important component of biological membranes. The thermodynamic parameters governing self-assembly of anionic phospholipids are deduced here from isothermal titration calorimetry. Heats of demicellization were determined for dioctanoyl phosphatidylglycerol (PG) and phosphatidylse...

journal_title：Biophysical journal

authors： Marsh D

更新日期：2012-03-07 00:00:00

abstract：:Polarized Fourier transform infrared spectroscopy has been used to study the structure of purple membrane from Halobacterium halobium. Membranes were oriented by drying a suspension of membrane fragments onto Irtran-4 slides. Dichroism measurements of the amide I, II and A peaks were used to find the average spatial o...

journal_title：Biophysical journal

authors： Rothschild KJ,Clark NA

更新日期：1979-03-01 00:00:00

abstract：:The effect of intracellular (i) and extracellular (o) Na+ on pre-steady-state transient current associated with Na+/Na+ exchange by the Na+/K+ pump was investigated in the vegetal pole of Xenopus oocytes. Current records in response to 40-ms voltage pulses from -180 to +100 mV in the absence of external Na+ were subtr...

journal_title：Biophysical journal

authors： Holmgren M,Rakowski RF

更新日期：2006-03-01 00:00:00

abstract：:Proteolysis of the multimeric blood coagulation protein von Willebrand Factor (VWF) by ADAMTS13 is crucial for prevention of microvascular thrombosis. ADAMTS13 cleaves VWF within the mechanosensitive A2 domain, which is believed to open under shear flow. In this study, we combine fluorescence correlation spectroscopy ...

journal_title：Biophysical journal

authors： Lippok S,Radtke M,Obser T,Kleemeier L,Schneppenheim R,Budde U,Netz RR,Rädler JO

更新日期：2016-02-02 00:00:00

abstract：:We present a method for calculating the configurational-dependent diffusion coefficient of a globular protein as a function of the global folding process. Using a coarse-grained structure-based model, we determined the diffusion coefficient, in reaction coordinate space, as a function of the fraction of native contact...

journal_title：Biophysical journal

authors： Oliveira RJ,Whitford PC,Chahine J,Wang J,Onuchic JN,Leite VB

更新日期：2010-07-21 00:00:00

abstract：:Motor enzymes use energy from ATP dephosphorylation to generate movement by a mechanical cycle, moving and pushing in one direction while attached to their cytoskeletal substrate, and recovering by moving relative to their substrate to a new attachment site. Mainstream models assert that movement while attached to the...

journal_title：Biophysical journal

authors： Brokaw CJ

更新日期：1997-08-01 00:00:00

abstract：:On the basis of its role in the analysis of mammillary compartmental systems, a matrix with non-zero elements in the first row, first column, and along the main diagonal and with zero elements elsewhere is called a mammillary matrix. It is pointed out that such matrices occur in a variety of biological problems includ...

journal_title：Biophysical journal

authors： Hearon JZ

更新日期：1964-01-01 00:00:00

abstract：:Thermodynamic analysis of metabolic networks has recently generated increasing interest for its ability to add constraints on metabolic network operation, and to combine metabolic fluxes and metabolite measurements in a mechanistic manner. Concepts for the calculation of the change in Gibbs energy of biochemical react...

journal_title：Biophysical journal

authors： Jol SJ,Kümmel A,Hatzimanikatis V,Beard DA,Heinemann M

更新日期：2010-11-17 00:00:00

abstract：:Freeze-fracture electron micrographs from degranulating cells show that the lumen of the secretory granule is connected to the extracellular compartment via large (20 to 150 nm diameter) aqueous pores. These exocytotic fusion pores appear to be made up of a highly curved bilayer that spans the plasma and granule membr...

journal_title：Biophysical journal

authors： Nanavati C,Markin VS,Oberhauser AF,Fernandez JM

更新日期：1992-10-01 00:00:00

abstract：:The role of distance-dependent anisotropic reactivity and molecular geometry in the vicinity of localized reaction centers in influencing the rate of bimolecular diffusion-controlled reactions is analyzed in detail, both analytically and numerically. The effect of local molecular shape is considered within the model o...

journal_title：Biophysical journal

authors： Shushin AI,Barzykin AV

更新日期：2001-12-01 00:00:00

abstract：:We have used the elastic neutron scattering technique to investigate the dynamics of the two main saccharidic components of starch: amylose and amylopectin. The measurements were carried out in the temperature range of 20 to 320 K and at different hydration levels from the dry state up to 0.47 g saccharide/g D(2)O. In...

journal_title：Biophysical journal

authors： Di Bari M,Cavatorta F,Deriu A,Albanese G

更新日期：2001-08-01 00:00:00

abstract：:Computational methods that predict three-dimensional structures from amino acid sequences have become increasingly accurate and have provided insights into structure-function relationships for proteins in the absence of structural data. However, the accuracy of computational structural models requires experimental app...

journal_title：Biophysical journal

authors： Tateyama M,Liu H,Yang AS,Cormier JW,Kass RS

更新日期：2004-03-01 00:00:00

abstract：:Cytochrome c3 (cyt c3) can mediate electron transport across phosphatidylcholine (PC)/cardiolipin (CL) and PC/phosphatidylglycerol (PG) membranes. A two-molecule process is involved in the electron transport across PC/CL membranes in the liquid-crystalline state. In contrast, a single-molecule process dominates the el...

journal_title：Biophysical journal

authors： Kim SM,Yamamoto T,Todokoro Y,Takayama Y,Fujiwara T,Park JS,Akutsu H

更新日期：2006-01-15 00:00:00

abstract：:Mechanical and two-dimensional (2D) x-ray diffraction studies suggest that during isometric steady-state contraction, strongly bound cross-bridges mostly occupy early states in the power stroke, whereas rigor or rigor-like cross-bridges could not be detected. However, it remained unclear whether cross-bridges accumula...

journal_title：Biophysical journal

authors： Radocaj A,Weiss T,Helsby WI,Brenner B,Kraft T

更新日期：2009-02-18 00:00:00

abstract：:Recently, our understanding of the structural basis of troponin-tropomyosin's Ca2+-triggered regulation of striated muscle contraction has advanced greatly, particularly via cryo-electron microscopy data. Compelling atomic models of troponin-tropomyosin-actin were published for both apo- and Ca2+-saturated states of t...

journal_title：Biophysical journal

authors： Tobacman LS

更新日期：2021-01-05 00:00:00

abstract：:We present a truly quantitative fluorescence recovery after photobleaching (FRAP) model for use with the confocal laser scanning microscope based on the photobleaching of a long line segment. The line FRAP method is developed to complement the disk FRAP method reported before. Although being more subject to the influe...

journal_title：Biophysical journal

authors： Braeckmans K,Remaut K,Vandenbroucke RE,Lucas B,De Smedt SC,Demeester J

更新日期：2007-03-15 00:00:00

abstract：:Branched cable voltage recording and voltage clamp analytical solutions derived in two previous papers are used to explore practical issues concerning voltage clamp. Single exponentials can be fitted reasonably well to the decay phase of clamped synaptic currents, although they contain many underlying components. The ...

journal_title：Biophysical journal

authors： Major G

更新日期：1993-07-01 00:00:00

abstract：:Slow stretch ramps (velocity: 0.17 fiber lengths s-1) were imposed during fused tetanic contractions of intact muscle fibers of the frog (1.4-3.0 degrees C; sarcomere length: 2.12-2.21 microns). Instantaneous force-extension relations were derived both under isometric conditions and during slow stretch by applying fas...

journal_title：Biophysical journal

authors： Månsson A

更新日期：1989-08-01 00:00:00