Abstract:
:The centromeric histone H3 variant centromeric protein A (CENP-A), whose sequence is the least conserved among all histone variants, is responsible for specifying the location of the centromere. Here, we present a comprehensive study of CENP-A nucleosome arrays by cryo-electron tomography. We see that CENP-A arrays have different biophysical properties than canonical ones under low ionic conditions, as they are more condensed with a 20% smaller average nearest-neighbor distance and a 30% higher nucleosome density. We find that CENP-A nucleosomes have a predominantly crossed DNA entry/exit site that is narrowed on average by 8°, and they have a propensity to stack face to face. We therefore propose that CENP-A induces geometric constraints at the nucleosome DNA entry/exit site to bring neighboring nucleosomes into close proximity. This specific property of CENP-A may be responsible for generating a fundamental process that contributes to increased chromatin fiber compaction that is propagated under physiological conditions to form centromeric chromatin.
journal_name
Biophys Jjournal_title
Biophysical journalauthors
Geiss CP,Keramisanou D,Sekulic N,Scheffer MP,Black BE,Frangakis ASdoi
10.1016/j.bpj.2014.01.005subject
Has Abstractpub_date
2014-02-18 00:00:00pages
875-82issue
4eissn
0006-3495issn
1542-0086pii
S0006-3495(14)00063-0journal_volume
106pub_type
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