当前位置： SCI文献检索 > BIOPHYSICAL JOURNAL期刊下所有文献 > Importance of residue 13 and the C-terminus for the structure and activity of the antimicrobial peptide aurein 2.2.

Importance of residue 13 and the C-terminus for the structure and activity of the antimicrobial peptide aurein 2.2.

Abstract：

:Previous studies on aurein 2.2 and 2.3 in DMPC/DMPG and POPC/POPG membranes have shown that bilayer thickness and phosphatidylglycerol content have a significant impact on the interaction of these peptides with membrane bilayers. Further examination with the DiSC(3)5 assay has indicated that aurein 2.2 induces greater membrane leakage than aurein 2.3 in Staphylococcus aureus C622. The only difference between these peptides is a Leu to Ile mutation at residue 13. To better understand the importance of this residue, the structure and activity of the L13A, L13F, and L13V mutants were investigated. In addition, we investigated a number of peptides with truncations at the C-terminus to determine whether the C-terminus, which contains residue 13, is crucial for antimicrobial activity. Solution circular dichroism results demonstrated that the L13F mutation and the truncation of the C-terminus by six residues resulted in decreased helical content, whereas the L13A or L13V mutation and the truncation of the C-terminus by three residues showed little to no effect on the structure. Oriented circular dichroism results demonstrated that only an extensive C-terminal truncation reduced the ability of the peptide to insert into lipid bilayers. (31)P NMR spectroscopy showed that all peptides disorder the headgroups. The implications of these results in terms of antimicrobial activity and the ability of these peptides to induce leakage in S. aureus are discussed. The results suggest that the presence of the 13th residue in aurein 2.2 is important for structure and activity, but the exact nature of residue 13 is less important as long as it is a hydrophobic residue.

journal_name

Biophys J

journal_title

Biophysical journal

authors

Cheng JT,Hale JD,Kindrachuk J,Jenssen H,Elliott M,Hancock RE,Straus SK

doi

10.1016/j.bpj.2010.08.077

subject

Has Abstract

pub_date

2010-11-03 00:00:00

pages

2926-35

issue

9

eissn

0006-3495

issn

1542-0086

pii

S0006-3495(10)01165-3

journal_volume

99

pub_type

杂志文章

相关文献

BIOPHYSICAL JOURNAL文献大全
  • Two-step folding of recombinant mitochondrial porin in detergent.

    abstract：:Precise information regarding the transmembrane topology of mitochondrial porin is essential for understanding the mechanisms by which this protein functions. Porin acts as a channel in the outer membrane and interacts with small solutes and proteins to regulate mitochondrial function. The acquisition of high-resoluti...

    journal_title：Biophysical journal

    pub_type： 杂志文章

    doi：10.1529/biophysj.107.115196

    authors： Bay DC,O'Neil JD,Court DA

    更新日期：2008-01-15 00:00:00

  • Dissecting the energies that stabilize sickle hemoglobin polymers.

    abstract：:Sickle hemoglobin forms long, multistranded polymers that account for the pathophysiology of the disease. The molecules in these polymers make significant contacts along the polymer axis (i.e., axial contacts) as well as making diagonally directed contacts (i.e., lateral contacts). The axial contacts do not engage the...

    journal_title：Biophysical journal

    pub_type： 杂志文章

    doi：10.1016/j.bpj.2013.09.032

    authors： Wang Y,Ferrone FA

    更新日期：2013-11-05 00:00:00

  • Electron-spin resonance study of aggregation of gramicidin in dipalmitoylphosphatidylcholine bilayers and hydrophobic mismatch.

    abstract：:The effect of aggregation of gramicidin A' (GA) on the phase structure of dipalmitoylphosphatidylcholine (DPPC) multilamellar vesicles was studied by cw-ESR using a chain-labeled lipid (16PC) at temperatures between 30 degrees and 45 degreesC that span the main phase transition of DPPC. Boundary lipids were observed o...

    journal_title：Biophysical journal

    pub_type： 杂志文章

    doi：10.1016/S0006-3495(99)77195-X

    authors： Ge M,Freed JH

    更新日期：1999-01-01 00:00:00

  • Octopus photoreceptor membranes. Surface charge density and pK of the Schiff base of the pigments.

    abstract：:The chromophore of octopus rhodopsin is 11-cis retinal, linked via a protonated Schiff base to the protein backbone. Its stable photoproduct, metarhodopsin, has all-trans retinal as its chromphore. The Schiff base of acid metarhodopsin (lambda max = 510 nm) is protonated, whereas that of alkaline metarhodopsin (lambda...

    journal_title：Biophysical journal

    pub_type： 杂志文章

    doi：10.1016/S0006-3495(90)82394-8

    authors： Koutalos Y,Ebrey TG,Gilson HR,Honig B

    更新日期：1990-08-01 00:00:00

  • Photoperturbation of the heme a3-CuB binuclear center of cytochrome c oxidase CO complex observed by Fourier transform infrared spectroscopy.

    abstract：:Purified cytochrome c oxidase CO complex from beef heart has been studied by Fourier transform infrared absorbance difference spectroscopy. Photolysis at 10-20 Kelvin results in dissociation of a3FeCO, formation of CuBCO, and perturbation of the a3-heme and CuB complex. The vibrational perturbation spectrum between 90...

    journal_title：Biophysical journal

    pub_type： 杂志文章

    doi：10.1016/S0006-3495(96)79304-9

    authors： Park S,Pan LP,Chan SI,Alben JO

    更新日期：1996-08-01 00:00:00

  • On the wobbling-in-cone analysis of fluorescence anisotropy decay.

    abstract：:Interpretation of fluorescence anisotropy decay for the case of restricted rotational diffusion often requires a model. To investigate the extent of model dependence, two models are compared: a strict cone model, in which a fluorescent probe wobbles uniformly within a cone, and a Gaussian model, where the stationary d...

    journal_title：Biophysical journal

    pub_type： 杂志文章

    doi：10.1016/S0006-3495(82)84692-4

    authors： Kinosita K Jr,Ikegami A,Kawato S

    更新日期：1982-02-01 00:00:00

  • Studies of impedance in cardiac tissue using sucrose gap and computer techniques. II. Circuit simulation of passive electrical properties and cell-to-cell transmission.

    abstract：:The impedance measured in a strip of heart tissue from the moth Hyalophora cecropia is fitted by circuit models of several configurations. The circuits include: (a) a single R-C circuit (b) a double R-C circuit (c) terminated transmission lines, and (d) a pattern of cells with cell-to-cell transmission paths. The last...

    journal_title：Biophysical journal

    pub_type： 杂志文章

    doi：10.1016/S0006-3495(74)70001-7

    authors： Stibitz GR,McCann FV

    更新日期：1974-02-01 00:00:00

  • Compressibilities and volume fluctuations of archaeal tetraether liposomes.

    abstract：:Bipolar tetraether lipids (BTLs) are abundant in crenarchaeota, which thrive in both thermophilic and nonthermophilic environments, with wide-ranging growth temperatures (4-108°C). BTL liposomes can serve as membrane models to explore the role of BTLs in the thermal stability of the plasma membrane of crenarchaeota. I...

    journal_title：Biophysical journal

    pub_type： 杂志文章

    doi：10.1016/j.bpj.2010.09.061

    authors： Chong PL,Sulc M,Winter R

    更新日期：2010-11-17 00:00:00

  • Binding of anionic lipids to at least three nonannular sites on the potassium channel KcsA is required for channel opening.

    abstract：:In addition to the annular or boundary lipids that surround the transmembrane surface of the potassium channel KcsA from Streptomyces lividans, x-ray crystallographic studies have detected one anionic lipid molecule bound at each protein-protein interface in the homotetrameric structure, at sites referred to as nonann...

    journal_title：Biophysical journal

    pub_type： 杂志文章

    doi：10.1529/biophysj.107.117507

    authors： Marius P,Zagnoni M,Sandison ME,East JM,Morgan H,Lee AG

    更新日期：2008-03-01 00:00:00

  • Penetratin-membrane association: W48/R52/W56 shield the peptide from the aqueous phase.

    abstract：:Using molecular dynamics simulations, we studied the mode of association of the cell-penetrating peptide penetratin with both a neutral and a charged bilayer. The results show that the initial peptide-lipid association is a fast process driven by electrostatic interactions. The homogeneous distribution of positively c...

    journal_title：Biophysical journal

    pub_type： 杂志文章

    doi：10.1529/biophysj.104.052787

    authors： Lensink MF,Christiaens B,Vandekerckhove J,Prochiantz A,Rosseneu M

    更新日期：2005-02-01 00:00:00

  • The electrical conductance of semipermeable membranes. I. A formal analysis.

    abstract：:A kinetic analysis of membrane conductance under conditions of stationary flow is presented. The semipermeable membrane is idealized as a homogeneous laminar phase separating ionic solutions on either side. It is assumed, without consideration of the mechanisms involved, that some ion species permeate the membrane whi...

    journal_title：Biophysical journal

    pub_type： 杂志文章

    doi：10.1016/S0006-3495(65)86757-1

    authors： Bruner LJ

    更新日期：1965-11-01 00:00:00

  • Changes in quantum yield of photosynthesis in the red alga Porphyridium cruentum caused by stepwise reduction in the intensity of light preferentially absorbed by the phycobilins.

    abstract：:This paper describes the relation between the quantum yield of photosynthesis in the red alga Porphyridium cruentum, and the spectral composition of light, changed by filtering white light through aqueous phycobilin solutions of increasing optical density. At sufficiently high densities of the filter solution, no meas...

    journal_title：Biophysical journal

    pub_type： 杂志文章

    doi：10.1016/s0006-3495(60)86876-2

    authors： THOMAS JB,GOVINDJEE

    更新日期：1960-09-01 00:00:00

  • Triphasic force dependence of E-selectin/ligand dissociation governs cell rolling under flow.

    abstract：:During inflammation, flowing leukocytes tether to and roll on vascular surfaces through the association and dissociation of selectin/ligand bonds. The interactions of P- and L- selectins with their respective ligands exhibit catch-slip bonds, such that increasing force initially prolongs and then shortens bond lifetim...

    journal_title：Biophysical journal

    pub_type： 杂志文章

    doi：10.1016/j.bpj.2010.05.040

    authors： Wayman AM,Chen W,McEver RP,Zhu C

    更新日期：2010-08-09 00:00:00

  • Prediction of solution structures of the Ca2+-bound gamma-carboxyglutamic acid domains of protein S and homolog growth arrest specific protein 6: use of the particle mesh Ewald method.

    abstract：:The solution structures of the N-terminal domains of protein S, a plasma vitamin K-dependent glycoprotein, and its homolog growth arrest specific protein 6 (Gas6) were predicted by molecular dynamics computer simulations. The initial structures were based on the x-ray crystallographic structure of the corresponding re...

    journal_title：Biophysical journal

    pub_type： 杂志文章

    doi：10.1016/S0006-3495(97)78215-8

    authors： Perera L,Li L,Darden T,Monroe DM,Pedersen LG

    更新日期：1997-10-01 00:00:00

  • Coarse-grained simulation studies of peptide-induced pore formation.

    abstract：:We investigate the interactions between lipid bilayers and amphiphilic peptides using a solvent-free coarse-grained simulation technique. In our model, each lipid is represented by one hydrophilic and three hydrophobic beads. The amphiphilic peptide is modeled as a hydrophobic-hydrophilic cylinder with hydrophilic cap...

    journal_title：Biophysical journal

    pub_type： 杂志文章

    doi：10.1529/biophysj.108.131300

    authors： Illya G,Deserno M

    更新日期：2008-11-01 00:00:00

  • Thermodynamics of phospholipid self-assembly.

    abstract：:Negatively charged phospholipids are an important component of biological membranes. The thermodynamic parameters governing self-assembly of anionic phospholipids are deduced here from isothermal titration calorimetry. Heats of demicellization were determined for dioctanoyl phosphatidylglycerol (PG) and phosphatidylse...

    journal_title：Biophysical journal

    pub_type： 杂志文章

    doi：10.1016/j.bpj.2012.01.049

    authors： Marsh D

    更新日期：2012-03-07 00:00:00

  • Quantifying the importance of protein conformation on ligand migration in myoglobin.

    abstract：:Myoglobin (Mb) is a model system for ligand binding and migration. The energy barriers (ΔG) for ligand migration in Mb have been studied in the past by experiment and theory and significant differences between different approaches were found. From experiment, it is known that Mb can assume a large number of conformati...

    journal_title：Biophysical journal

    pub_type： 杂志文章

    doi：10.1016/j.bpj.2011.10.058

    authors： Plattner N,Meuwly M

    更新日期：2012-01-18 00:00:00

  • Inactivation of L-type Ca channels in embryonic chick ventricle cells: dependence on the cytoskeletal agents colchicine and taxol.

    abstract：:This article shows that colchicine and taxol strongly influence the kinetics of L-type Ca channels in intact cardiac cells, and it suggests a mechanism for this action. It is known that colchicine disassociates microtubules into tubulin, and that taxol stabilizes microtubules. We have found that colchicine increases t...

    journal_title：Biophysical journal

    pub_type： 杂志文章

    doi：10.1016/S0006-3495(94)80715-5

    authors： Galli A,DeFelice LJ

    更新日期：1994-12-01 00:00:00

  • Refinement of OprH-LPS Interactions by Molecular Simulations.

    abstract：:The outer membrane (OM) of Gram-negative bacteria is composed of lipopolysaccharide (LPS) in the outer leaflet and phospholipids in the inner leaflet. The outer membrane protein H (OprH) of Pseudomonas aeruginosa provides an increased stability to the OMs by directly interacting with LPS. Here we report the influence ...

    journal_title：Biophysical journal

    pub_type： 杂志文章

    doi：10.1016/j.bpj.2016.12.006

    authors： Lee J,Patel DS,Kucharska I,Tamm LK,Im W

    更新日期：2017-01-24 00:00:00

  • Surface charge movements of purple membrane during light-dark adaptation.

    abstract：:The difference in the surface charge distribution between light-adapted and dark-adapted purple membranes was investigated with electric dichroism measurements from approximately pH 5 to pH 11. Purple membrane sheets in solution are oriented in a weak electric field by their permanent dipole moment, which is due to th...

    journal_title：Biophysical journal

    pub_type： 杂志文章

    doi：10.1016/S0006-3495(86)83454-3

    authors： Otomo J,Ohno K,Takeuchi Y,Ikegami A

    更新日期：1986-08-01 00:00:00

  • Influence of Ganglioside GM1 Concentration on Lipid Clustering and Membrane Properties and Curvature.

    abstract：:Gangliosides are a class of glycosphingolipids (GSLs) with amphiphilic character that are found at the outer leaflet of the cell membranes, where their ability to organize into special domains makes them vital cell membrane components. However, a molecular understanding of GSL-rich membranes in terms of their clustere...

    journal_title：Biophysical journal

    pub_type： 杂志文章

    doi：10.1016/j.bpj.2016.09.021

    authors： Patel DS,Park S,Wu EL,Yeom MS,Widmalm G,Klauda JB,Im W

    更新日期：2016-11-01 00:00:00

  • Temperature dependence of IP3-mediated local and global Ca2+ signals.

    abstract：:We examined the effect of temperature (12-40°C) on local and global Ca2+ signals mediated by inositol trisphosphate receptor/channels (IP3R) in human neuroblastoma (SH-SY5Y) cells. The amplitudes and spatial spread of local signals arising from single IP3R (blips) and clusters of IP3R (puffs) showed little temperature...

    journal_title：Biophysical journal

    pub_type： 杂志文章

    doi：10.1016/j.bpj.2012.12.024

    authors： Dickinson GD,Parker I

    更新日期：2013-01-22 00:00:00

  • Lipid-Conjugated Rigidochromic Probe Discloses Membrane Alteration in Model Cells of Krabbe Disease.

    abstract：:The plasma membrane of cells has a complex architecture based on the bidimensional liquid-crystalline bilayer arrangement of phospho- and sphingolipids, which in turn embeds several proteins and is connected to the cytoskeleton. Several studies highlight the spatial membrane organization into more ordered (Lo or lipid...

    journal_title：Biophysical journal

    pub_type： 杂志文章

    doi：10.1016/j.bpj.2018.11.3141

    authors： Abbandonato G,Storti B,Tonazzini I,Stöckl M,Subramaniam V,Montis C,Nifosì R,Cecchini M,Signore G,Bizzarri R

    更新日期：2019-02-05 00:00:00

  • Intestinal Villus Structure Contributes to Even Shedding of Epithelial Cells.

    abstract：:In the intestinal epithelium, proliferated epithelial cells ascend the crypts and villi and shed at the villus tips into the gut lumen. In this study, we theoretically investigate the roles of the villi on cell turnover. We present a stochastic model that focuses on the duration over which cells migrate the shortest p...

    journal_title：Biophysical journal

    pub_type： 杂志文章

    doi：10.1016/j.bpj.2021.01.003

    authors： Kai Y

    更新日期：2021-01-13 00:00:00

  • Intrinsic cytosolic calcium buffering properties of single rat cardiac myocytes.

    abstract：:Intracellular passive Ca2+, buffering was measured in voltage-clamped rat ventricular myocytes. Cells were loaded with indo-1 (K+ salt) to an estimated cytosolic concentration of 44 +/- 5 microM (Mean +/- SEM, n = 5), and accessible cell volume was estimated to be 24.5 +/- 3.6 pl. Ca2+ transport by the sarcoplasmic re...

    journal_title：Biophysical journal

    pub_type： 杂志文章

    doi：10.1016/S0006-3495(94)80652-6

    authors： Berlin JR,Bassani JW,Bers DM

    更新日期：1994-10-01 00:00:00

  • The role of diffusion in enzyme kinetics.

    abstract：:A discussion is given of the general role of diffusion in enzyme kinetics based upon a rigorous theory for bimolecular association and dissociation steps that was presented previously, and criteria are formulated for the dependence of the over-all rate on medium viscosity. With these criteria it is possible to conclud...

    journal_title：Biophysical journal

    pub_type： 杂志文章

    doi：10.1016/S0006-3495(70)86331-7

    authors： Schurr JM

    更新日期：1970-08-01 00:00:00

  • The exocytotic fusion pore modeled as a lipidic pore.

    abstract：:Freeze-fracture electron micrographs from degranulating cells show that the lumen of the secretory granule is connected to the extracellular compartment via large (20 to 150 nm diameter) aqueous pores. These exocytotic fusion pores appear to be made up of a highly curved bilayer that spans the plasma and granule membr...

    journal_title：Biophysical journal

    pub_type： 杂志文章

    doi：10.1016/S0006-3495(92)81679-X

    authors： Nanavati C,Markin VS,Oberhauser AF,Fernandez JM

    更新日期：1992-10-01 00:00:00

  • Native ligands change integrin sequestering but not oligomerization in raft-mimicking lipid mixtures.

    abstract：:Distinct lipid environments, including lipid rafts, are increasingly recognized as a crucial factor affecting membrane protein function in plasma membranes. Unfortunately, an understanding of their role in membrane protein activation and oligomerization has remained elusive due to the challenge of characterizing these...

    journal_title：Biophysical journal

    pub_type： 杂志文章

    doi：10.1016/j.bpj.2011.08.040

    authors： Siegel AP,Kimble-Hill A,Garg S,Jordan R,Naumann CA

    更新日期：2011-10-05 00:00:00

  • Mechanics and Buckling of Biopolymeric Shells and Cell Nuclei.

    abstract：:We study a Brownian dynamics simulation model of a biopolymeric shell deformed by axial forces exerted at opposing poles. The model exhibits two distinct, linear force-extension regimes, with the response to small tensions governed by linear elasticity and the response to large tensions governed by an effective spring...

    journal_title：Biophysical journal

    pub_type： 杂志文章

    doi：10.1016/j.bpj.2017.08.034

    authors： Banigan EJ,Stephens AD,Marko JF

    更新日期：2017-10-17 00:00:00

  • Theoretical analysis of membrane tension in moving cells.

    abstract：:Lateral tension in cell plasma membranes plays an essential role in regulation of a number of membrane-related intracellular processes and cell motion. Understanding the physical factors generating the lateral tension and quantitative determination of the tension distribution along the cell membrane is an emerging top...

    journal_title：Biophysical journal

    pub_type： 杂志文章

    doi：10.1016/j.bpj.2013.11.009

    authors： Schweitzer Y,Lieber AD,Keren K,Kozlov MM

    更新日期：2014-01-07 00:00:00